Predictive role of node-rads score in patients with prostate cancer candidates for radical prostatectomy with extended lymph node dissection: comparative analysis with validated nomograms

Background and objectives: The Reporting and Data System (RADS) have been used in the attempts to standardize the results of oncological scans in different scenarios, such as lymph nodes, adding configuration criteria to size determination. We analyze the predictive value of preoperative Node-RADS determination at imaging for pelvic lymph node (PLN) involvement in cases of prostate cancer (PC) considered for radical prostatectomy (RP) with extended lymph node dissection (eLND) and we compare it with validate predictive nomograms (MSKCC, Briganti and Gandaglia). Methods: 150 patients with a histological diagnosis of PC (high risk or intermediate with an estimated risk for pN+ higher than 5% using the Briganti or 7% using the Gandaglia nomogram) submitted for RP with an ePLND from 2018 and 2021 were retrospectively examined. Node-RADS determination was performed in all cases using the preoperative magnetic resonance (MR), performed by a radiologist blinded for pathologic results and compared with the MSKCC, Briganti 2012, Gandaglia 2017 and Gandaglia 2019 nomograms. Results: PLN involvement at final pathology (pN+) was found in 36/150 (24.0%) of cases and the mean percentage of positive LNs in pN+ cases was 15.90 ± 13.40. The mean number of PLNs removed at RP was similar (p = 0.188) between pN0 (23.9 ± 8.0) and pN+ (25.3 ± 8.0) cases. Considering a Node RADS 4-5 positive and a Node RADS 1-2 negative, the PPV was 100% and the NPV was 79.6%. A Node RADS score 4-5 showed a lower sensitivity (0.167 versus 0.972, 1.000, 0.971, 0.960 respectively), a higher specificity (1.000 versus 0.079, 0.096, 0.138, 0.186 respectively) and a similar AUC (0.583 versus 0.591, 0.581, 0.574, 0.597 respectively) when compared to MSKCC, Briganti 2012, Gandaglia 2017 and Gandaglia 2019 nomograms. Conclusions: Our evaluation suggests that Node RADS score, combining configuration criteria to size determination could improve specificity in terms of pathologic PLN prediction but a very low sensitivity has been also described

Findings of the WMT 2021 Biomedical Translation Shared Task: Summaries of Animal Experiments as New Test Set

In the sixth edition of the WMT Biomedical Task, we addressed a total of eight language pairs, namely English/German, English/French, English/Spanish, English/Portuguese, English/Chinese, English/Russian, English/Italian, and English/Basque. Further, our tests were composed of three types of textual test sets. New to this year, we released a test set of summaries of animal experiments, in addition to the test sets of scientific abstracts and terminologies. We received a total of 107 submissions from 15 teams from 6 countries

Digoxin reveals a functional connection between HIV-1 integration preference and T-cell activation

HIV-1 integrates more frequently into transcribed genes, however the biological significance of HIV-1 integration targeting has remained elusive. Using a selective high-throughput chemical screen, we discovered that the cardiac glycoside digoxin inhibits wild-type HIV-1 infection more potently than HIV-1 bearing a single point mutation (N74D) in the capsid protein. We confirmed that digoxin repressed viral gene expression by targeting the cellular Na+/K+ ATPase, but this did not explain its selectivity. Parallel RNAseq and integration mapping in infected cells demonstrated that digoxin inhibited expression of genes involved in T-cell activation and cell metabolism. Analysis of >400,000 unique integration sites showed that WT virus integrated more frequently than N74D mutant within or near genes susceptible to repression by digoxin and involved in T-cell activation and cell metabolism. Two main gene networks down-regulated by the drug were CD40L and CD38. Blocking CD40L by neutralizing antibodies selectively inhibited WT virus infection, phenocopying digoxin. Thus the selectivity of digoxin depends on a combination of integration targeting and repression of specific gene networks. The drug unmasked a functional connection between HIV-1 integration and T-cell activation. Our results suggest that HIV-1 evolved integration site selection to couple its early gene expression with the status of target CD4+ T-cells, which may affect latency and viral reactivation

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Compressive sensing reconstruction for complex system: A hardware/software approach

Today, a number of applications need to process large bandwidth signals. These applications frequently require the use of fast ADCs and very efficient DSP structures that are difficult to design. An interesting solution for facing these issues is the Compressive Sensing (CS) method, which, assuming to know some properties of the signal, allows to reduce the sampling rate well below the Nyquist rate. A negative aspect of CS is the need to introduce an additional element for the reconstruction the sampled signal. This reconstruction requires techniques that generally have an high computational cost, representing a critical element for a real-time implementation of CS systems. In this work we present the implementation of one of these reconstruction algorithms, named Orthogonal Matching Pursuit (OMP). This algorithm involves heavy computational cost (in particular for the matrix computation), which limits its use in the case of a strictly real-time applications, as in the case of radar systems. To overcome this limitation authors propose a solution that uses for the implementation a mixed software/hardware approach. The proposed architecture was implemented on the Xilinx ZYNQ FPGA. The experimental results show a significant speed-up of the algorithm

2AIRTC: The Amharic Adhoc Information Retrieval Test Collection

International audienceEvaluation is highly important for designing, developing, and maintaining information retrieval (IR) systems. The IR community has developed shared tasks where evaluation framework, evaluation measures and test collections have been developed for different languages. Although Amharic is the official language of Ethiopia currently having an estimated population of over 110 million, it is one of the under-resourced languages and there is not yet Amharic adhoc IR test collection. In this paper, we promote the monolingual Amharic IR test collection that we built for the IR community. Following the framework of Cranfield project and TREC, the collection that we named 2AIRTC consists of 12,583 documents, 240 topics and the corresponding relevance judgments

Extended CLEF eHealth 2013-2015 IR Test Collection

This package contains an extended version of the test collection used in the CLEF eHealth Information Retrieval tasks in 2013--2015. Compared to the original version, it provides complete query translations into Czech, French, German, Hungarian, Polish, Spanish and Swedish and additional relevance assessment