7,302 research outputs found
Antioxidant activities in vitro of water and liposoluble extracts obtained by different species of edible insects and invertebrates
A new global interest in entomophagy, the practice of eating insects, and invertebrates, arise from the impellent necessity of preserving agriculture resources and to obtain a drastic reduction of the ecological impact of animal food on the planet. The composite nutritional content, direct consequences of a plant-based feeding, associated with the undoubtedly ecological properties, suggest for insects a role as sustainable and functional foods. We aim to investigate the ability of water and liposoluble extracts, obtained by 12 commercially available edible insects and two invertebrates, to display an antioxidant effect in vitro. Results show that water-soluble extracts of grasshoppers, silkworm, and crickets display the highest values of antioxidant capacity (TEAC), 5-fold higher than fresh orange juice, while evening cicada, giant water bugs, Thai zebra tarantula, and black scorpions have negligible values. Grasshoppers, African caterpillars, and crickets have the highest levels of reducing power (FRAP), double than fresh orange juice. Grasshoppers, black ants, and mealworms contain the highest levels of total polyphenols, while Thai zebra tarantula, black scorpions, and giant water bugs are positioned at the bottom of the ranking. The liposoluble fraction of silkworm, evening cicada, and African caterpillars shows highest level of TEAC, twice than olive oil, while Thai zebra tarantula, palm worm, and black ants are placed at the bottom of the ranking. Edible insects and invertebrates represent a potential source of antioxidant ingredients with an efficiency related to their taxonomy and eating habits. More evidences are needed in order to understand if the practice of eating insects and invertebrates might contribute to modulate oxidative stress in humans
Functional properties of edible insects: a systematic review
: Consumption of edible insects has been widely suggested as an environmentally sustainable substitute for meat to reduce GHG emissions. However, the novel research field for edible insects rely on the content of bioactive ingredients and on the ability to induce a functional effect in humans. The goal of this manuscript was to review the available body of evidence on the properties of edible insects in modulating oxidative and inflammatory stress, platelet aggregation, lipid and glucose metabolism and weight control. A search for literature investigating the functional role of edible insects was carried out in the PUBMED database using specific keywords. A total of 55 studies, meeting inclusion criteria after screening, were divided on the basis of the experimental approach: in vitro studies, cellular models/ex vivo studies or in vivo studies. In the majority of the studies, insects demonstrated the ability to reduce oxidative stress, modulate antioxidant status, restore the impaired activity of antioxidant enzymes and reduce markers of oxidative damage. Edible insects displayed anti-inflammatory activity reducing cytokines and modulating specific transcription factors. Results from animal studies suggest that edible insects can modulate lipid and glucose metabolism. The limited number of studies focused on the assessment of anticoagulation activity of edible insects make it difficult to draw conclusions. More evidence from dietary intervention studies in humans is needed to support the promising evidence from in vitro and animal models about the functional role of edible insects consumption
Radical scavenging activity of olive oil phenolic antioxidants in oil or water phase during the oxidation of O/W emulsions: An oxidomics approach
Omics approaches are recently being applied also in food lipid oxidation, to increase knowledge of oxidation and antioxidation mechanisms. The so-called oxidomics throws a wider spot of light on the complex patterns of reactions taking place in food lipids, especially in dispersed systems. This research aimed to investigate the radical scavenging activity of olive oil phenolic antioxidants (OPAs) in O/W emulsions, as affected by the phase in which they were added. This allowed one to assess whether different behaviors could be expected from antioxidants originally present in phenolic-rich olive oils compared to natural antioxidants added in the water phase during emulsion production. Hydroperoxide decomposition kinetics and the analysis of volatile pattern provided an outline of antioxidation mechanisms. Though being effective in slowing down oxidation when added both in the oil and water phase, OPAs interfered in different ways with oxidation pathways, based on the phase in which they were added. OPAs added to the water phase were more effective in slowing down hydroperoxide decomposition due to the hydrophilic radical initiator. On the other hand, OPAs present in the oil were more effective in preventing radical propagation, with relevant consequences on the volatile pattern
Food-borne Lactiplantibacillus plantarum protect normal intestinal cells against inflammation by modulating reactive oxygen species and IL-23/IL-17 axis
Food-associated Lactiplantibacillus plantarum (Lpb. plantarum) strains, previously classified as Lactobacillus plantarum, are a promising strategy to face intestinal inflammatory diseases. Our study was aimed at clarifying the protective role of food-borne Lpb. plantarum against inflammatory damage by testing the scavenging microbial ability both in selected strains and in co-incubation with normal mucosa intestinal cells (NCM460). Here, we show that Lpb. plantarum endure high levels of induced oxidative stress through partially neutralizing reactive oxygen species (ROS), whereas they elicit their production when co-cultured with NCM460. Moreover, pre-treatment with food-borne Lpb. plantarum significantly reduce pro-inflammatory cytokines IL-17F and IL-23 levels in inflamed NCM460 cells. Our results suggest that food-vehicled Lpb. plantarum strains might reduce inflammatory response in intestinal cells by directly modulating local ROS production and by triggering the IL-23/IL-17 axis with future perspectives on health benefits in the gut derived by the consumption of functional foods enriched with selected strains
Bioactive potential of minor italian olive genotypes from apulia, sardinia and abruzzo
This research focuses on the exploration, recovery and valorization of some minor Italian olive cultivars, about which little information is currently available. Autochthonous and unexplored germplasm has the potential to face unforeseen changes and thus to improve the sustainability of the whole olive system. A pattern of nine minor genotypes cultivated in three Italian regions has been molecularly fingerprinted with 12 nuclear microsatellites (SSRs), that were able to unequivocally identify all genotypes. Moreover, some of the principal phenolic compounds were determined and quantified in monovarietal oils and the expression levels of related genes were also investigated at different fruit developmental stages. Genotypes differed to the greatest extent in the content of oleacein (3,4-DHPEA-EDA) and total phenols. Thereby, minor local genotypes, characterized by stable production and resilience in a low-input agro-system, can provide a remarkable contribution to the improvement of the Italian olive production chain and can become very profitable from a socio-economic point of view
Infants hospitalized for Bordetella pertussis infection commonly have respiratory viral coinfections
Background: Whether viral coinfections cause more severe disease than Bordetella pertussis (B. pertussis) alone remains
unclear. We compared clinical disease severity and sought clinical and demographic differences between infants with
B. pertussis infection alone and those with respiratory viral coinfections. We also analyzed how respiratory infections
were distributed during the 2 years study.
Methods: We enrolled 53 infants with pertussis younger than 180 days (median age 58 days, range 17â109 days, 64.
1% boys), hospitalized in the Pediatric Departments at âSapienzaâ University Rome and Bambino GesĂč Childrenâs
Hospital from August 2012 to November 2014. We tested in naso-pharyngeal washings B. pertussis and 14 respiratory
viruses with real-time reverse-transcriptase-polymerase chain reaction. Clinical data were obtained from hospital
records and demographic characteristics collected using a structured questionnaire.
Results: 28/53 infants had B. pertussis alone and 25 viral coinfection: 10 human rhinovirus (9 alone and 1 in coinfection
with parainfluenza virus), 3 human coronavirus, 2 respiratory syncytial virus. No differences were observed in clinical
disease severity between infants with B. pertussis infection alone and those with coinfections. Infants with B. pertussis
alone were younger than infants with coinfections, and less often breastfeed at admission.
Conclusions: In this descriptive study, no associations between clinical severity and pertussis with or without
co-infections were found
In Vitro Innovation of Tendon Tissue Engineering Strategies.
Tendinopathy is the term used to refer to tendon disorders. Spontaneous adult tendon healing results in scar tissue formation and fibrosis with suboptimal biomechanical properties, often resulting in poor and painful mobility. The biomechanical properties of the tissue are negatively affected. Adult tendons have a limited natural healing capacity, and often respond poorly to current treatments that frequently are focused on exercise, drug delivery, and surgical procedures. Therefore, it is of great importance to identify key molecular and cellular processes involved in the progression of tendinopathies to develop effective therapeutic strategies and drive the tissue toward regeneration. To treat tendon diseases and support tendon regeneration, cell-based therapy as well as tissue engineering approaches are considered options, though none can yet be considered conclusive in their reproduction of a safe and successful long-term solution for full microarchitecture and biomechanical tissue recovery. In vitro differentiation techniques are not yet fully validated. This review aims to compare different available tendon in vitro differentiation strategies to clarify the state of art regarding the differentiation process
A comprehensive interpretative model of slow slip events on Mt. Etnaâs eastern flank
Starting off from a review of previous literature on kinematic models of the unstable eastern
flank of Mt. Etna, we propose a new model. The model is based on our analysis of a large quantity of multidisciplinary
data deriving from an extensive and diverse network of INGV monitoring devices deployed
along the slopes of the volcano. Our analysis had a twofold objective: first, investigating the origin of the
recently observed slow-slip events on the eastern flank of Mt. Etna; and second, defining a general kinematic
model for the instability of this area of the volcano. To this end, we investigated the 2008â2013
period using data collected from different geochemical, geodetic, and seismic networks, integrated with
the tectonic and geologic features of the volcano and including the volcanic activity during the observation
period. The complex correlations between the large quantities of multidisciplinary data have given us the
opportunity to infer, as outlined in this work, that the fluids of volcanic origin and their interrelationship
with aquifers, tectonic and morphological features play a dominant role in the large scale instability of the
eastern flank of Mt. Etna. Furthermore, we suggest that changes in the strain distribution due to volcanic
inflation/deflation cycles are closely connected to changes in shallow depth fluid circulation. Finally, we propose
a general framework for both the short and long term modeling of the large flank displacements
observed.Published635â6581IT. Reti di monitoraggio e OsservazioniJCR Journalrestricte
FFAT motif phosphorylation controls formation and lipid transfer function of interâorganelle contacts
Organelles are physically connected in membrane contact sites. The endoplasmic reticulum possesses three major receptors, VAPâA, VAPâB, and MOSPD2, which interact with proteins at the surface of other organelles to build contacts. VAPâA, VAPâB, and MOSPD2 contain an MSP domain, which binds a motif named FFAT (two phenylalanines in an acidic tract). In this study, we identified a nonâconventional FFAT motif where a conserved acidic residue is replaced by a serine/threonine. We show that phosphorylation of this serine/threonine is critical for nonâconventional FFAT motifs (named PhosphoâFFAT) to be recognized by the MSP domain. Moreover, structural analyses of the MSP domain alone or in complex with conventional and PhosphoâFFAT peptides revealed new mechanisms of interaction. Based on these new insights, we produced a novel prediction algorithm, which expands the repertoire of candidate proteins with a PhosphoâFFAT that are able to create membrane contact sites. Using a prototypical tethering complex made by STARD3 and VAP, we showed that phosphorylation is instrumental for the formation of ERâendosome contacts, and their sterol transfer function. This study reveals that phosphorylation acts as a general switch for interâorganelle contacts
- âŠ