Helminth communities of wood mouse (Apodemus sylvaticus) on the river Avena (Calabria, southern Italy)

Helminth parasites of woodmouse Apodemus sylvaticus were studied from May 1993 through to June 1994 on the River Avena (Calabria, southern Italy), which, as other Mediterranenan rivers, is dry for most of the year. Trapping sites were located in three different habitats of the river: slopes, shores and the riverbed. A total of 106 animals was captured and screened for the presence of helminths. Five endoparasitic species were detected: Corrigia vitta, Rictularia proni, Heligmosomoides polygyrus, Syphacia stroma and S. frederici. Four helminth species were recorded from the riverbed and shore sections, whereas only H. polygyrus and S. frederici were found on the slopes. Syphacia frederici occurred at every trapping site. The total prevalence was 25.5% and total mean intensity was 27.2 parasites/mouse. No statistical significant difference was found for both the prevalence and mean intensity of infection between either habitats or sexes, suggesting that, from the point of view of the helminth community, the study area should be considered as a single habitat

Preparazione di bifenoli naturali funzionalizzati con unità fotosensibili reversibili

Il lavoro di ricerca ha voluto investigare la funzionalizzazione di bifenili naturali al fine di una loro applicazione nel campo dei materiali organici fotosensibili reversibili, appartenenti alla classe delle molecole switches

Validation of a Commercial Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Anisakis spp. DNA in Processed Fish Products

Parasites belonging to the Anisakis genera are organisms of interest for human health because they are responsible for the Anisakiasis zoonosis, caused by the ingestion of raw or undercooked fish. Furthermore, several authors have reported this parasite to be a relevant inducer of acute or chronic allergic diseases. In this work, a rapid commercial system based on Loop-Mediated Isothermal Amplification (LAMP) was optimised and validated for the sensitive and rapid detection of Anisakis spp. DNA in processed fish products. The specificity and sensitivity of the LAMP assay for processed fish samples experimentally infected with Anisakis spp. larvae and DNA were determined. The LAMP system proposed in this study was able to give positive amplification for all the processed fish samples artificially contaminated with Anisakis spp., giving sensitivity values equal to 100%. Specificity tests provided no amplification for the Contracaecum, Pseudoterranova, or Hysterothylacium genera and uninfected samples. The limit of detection (LOD) of the LAMP assay proposed was 102 times lower than the real-time PCR method compared. To the best of our knowledge, this is the first report regarding the application of the LAMP assay for the detection of Anisakis spp. in processed fish products. The results obtained indicate that the LAMP assay validated in this work could be a reliable, easy-to-use, and convenient tool for the rapid detection of Anisakis DNA in fish product inspection

Residues of 165 pesticides in citrus fruits using LC-MS/MS: a study of the pesticides distribution from the peel to the pulp

Abstract A sensitive LC–ESI-MS/MS method was developed for the determination of 165 pesticides in 50 citrus fruit samples collected in Sicily. Moreover, an evaluation of pesticides levels in the citrus layers (peel, albedo, and pulp) was carried out. The method presented acceptable trueness, precision, and linearity with LOQ of 5 μg/kg. The results obtained showed a high frequency of fungicides class pesticides in all the citrus samples examined (>95%) with the highest concentrations in the peel (4468 µg/Kg). A significant difference of concentrations was found between the layers of the citrus fruits analysed (p < 0.05). In particular, the peel and albedo present higher pesticides significantly higher than the pulp. Our findings confirming the widespread use of these substances in citrus cultivation and suggesting the importance of pesticides analysis in all the citrus fruit layers separately, considering the different interactions between the physicochemical characteristics of the matrices and the pesticides

Fisheries and aquaculture by-products modulate growth, body composition, and omega-3 polyunsaturated fatty acid content in black soldier fly (Hermetia illucens) larvae

The black soldier fly (BSF) (Hermetia illucens L.) is one of the most promising species for the production of ingredients, mainly protein, useful for animal feed formulation, owing to its ability to convert organic waste into biomass with a high nutritional value. However, the low percentage of n-3 series polyunsaturated fatty acids (PUFAs) in its fatty acid profile is a limiting factor for the utilization of BSF in fish feed. Recent studies have highlighted that wastes derived from different agro-food value chains could affect the nutritional composition of BSF larvae, depending on the composition of the wastes used as larvae-growing substrate. Due to the significant amount of n-3 PUFA in marine products, both in edible produce and in by-products, in this study, different sources of fish by-products were included in experimental diets for BSF to evaluate the effect of its addition on the final nutritional profile of BSF pre-pupae. One control diet and five experimental diets were prepared to feed the BSF larvae: wheat bran as the control diet (diet B), bycatch from Mediterranean trawl fisheries (diet F), Parapenaeus longirostris processing by-products (diet S), aquaculture processing by-products (diet R), Thunnus albacares processing by-products (diet T), and Engraulis encrasicolus processing by-products (diet A). In this study, the effects of the different diets were analyzed on the growth, body composition, and fatty acid profile of BSF larvae and pre-pupae. The obtained results showed that the different experimental diets affected total lipids content and fatty acids composition, when compared with the control. A significant increase in eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) in BSF larvae and pre-pupae fed with all fish by-products was observed when compared with those fed with diet B, in particular in larvae and pre-pupae fed with diet A, demonstrating that the utilization of fish processing by-products is a suitable solution for improving the nutritional value of insects as ingredients in aqua feeds. The reuse of marine by-products can contribute to the industry’s “zero waste” goal, increasing the sustainability of the fishery value chain and the formulation of new valuable products

Indagine statistica su acquisti e vendite dei principali prodotti alimentari locali nei Siti di Interesse Nazionale (SIN)

L’indagine nasce dalla collaborazione tra l’Istituto Zooprofilattico Sperimentale della Sicilia (IZS Sicilia) e l’Istituto per lo Studio degli Impatti Antropici e Sostenibilità in Ambiente Marino del Consiglio Nazionale delle Ricerche (IAS-CNR) nell’ambito del progetto CISAS (Centro Internazionale di Studi Avanzati su Ambiente, Ecosistema e Salute Umana). In CISAS sono state svolte attività indirizzate alla valutazione del rischio sanitario per le popolazioni residenti nelle aree dei Siti di Interesse Nazionale (SIN) di Augusta-Melilli-Priolo (SIN 1), di Milazzo (SIN 2) e di Crotone (SIN 3) caratterizzati da un forte degrado ambientale e da fenomeni di impatto umano di varia origine, sostanzialmente causati dallo sviluppo di importanti attività industriali (Giosuè et al., 2020). In questo scenario, considerati anche i risultati emersi da diversi rapporti sia italiani che Europei (ISMEA, 2019), i quali evidenziano una crescita di vendite dei prodotti di origine locale (certificati con marchi quali ad es. DOP, IGP, ecc..), spesso a km zero, si è ritenuto di fondamentale importanza indagare sul mercato dei prodotti di origine vegetale e animale provenienti dai tre SIN e destinati al mercato locale. Inoltre, tenendo in considerazione che negli ultimi anni la Grande Distribuzione Organizzata (GDO) ha avviato processi di acquisto e vendita di prodotti locali, prevedendo dei disciplinari di produzione, spesso particolarmente rigidi nel garantire la sicurezza alimentare, l’IZS Sicilia ha ritenuto di notevole importanza realizzare un’indagine esplorativa sui flussi di vendita e acquisti di prodotti freschi locali proprio nell’ambito della GDO, al fine di reperire informazioni più puntuali per implementare le valutazioni sul rischio delle popolazioni residenti nel SIN o nelle aree limitrofe. L’indagine è stata condotta attraverso interviste ai responsabili commerciali delle principali insegne della GDO, identificati su base campionaria nei SIN di Augusta Melilli Priolo, di Milazzo e di Crotone

Helminth communities of wood mouse (<em>Apodemus sylvaticus</em>) on the river Avena (Calabria, southern Italy)

Helminth parasites of woodmouse <em>Apodemus sylvaticus</em> were studied from May 1993 through to June 1994 on the River Avena (Calabria, southern Italy), which, as other Mediterranenan rivers, is dry for most of the year. Trapping sites were located in three different habitats of the river: slopes, shores and the riverbed. A total of 106 animals was captured and screened for the presence of helminths. Five endoparasitic species were detected: <em>Corrigia vitta</em>, <em>Rictularia proni</em>, <em>Heligmosomoides polygyrus</em>, <em>Syphacia stroma</em> and <em>S. frederici</em>. Four helminth species were recorded from the riverbed and shore sections, whereas only <em>H. polygyrus</em> and <em>S. frederici</em> were found on the slopes. <em>Syphacia frederici</em> occurred at every trapping site. The total prevalence was 25.5% and total mean intensity was 27.2 parasites/mouse. No statistical significant difference was found for both the prevalence and mean intensity of infection between either habitats or sexes, suggesting that, from the point of view of the helminth community, the study area should be considered as a single habitat

Helminth communities of wood mouse (Apodemus sylvaticus) on the river Avena (Calabria, southern Italy)

Helminth parasites of woodmouse Apodemus sylvaticus were studied from May 1993 through to June 1994 on the River Avena (Calabria, southern Italy), which, as other Mediterranenan rivers, is dry for most of the year. Trapping sites were located in three different habitats of the river: slopes, shores and the riverbed. A total of 106 animals was captured and screened for the presence of helminths. Five endoparasitic species were detected: Corrigia vitta, Rictularia proni, Heligmosomoides polygyrus, Syphacia stroma and S. frederici. Four helminth species were recorded from the riverbed and shore sections, whereas only H. polygyrus and S. frederici were found on the slopes. Syphacia frederici occurred at every trapping site. The total prevalence was 25.5% and total mean intensity was 27.2 parasites/mouse. No statistical significant difference was found for both the prevalence and mean intensity of infection between either habitats or sexes, suggesting that, from the point of view of the helminth community, the study area should be considered as a single habitat