Effect of <i>Excoecaria agallocha</i> leaves against <i>Aeromonas hydrophila</i> in marine ornamental fish, <i>Amphiprion sebae</i>

76-82Present study was performed to validate the potential of mangrove, Excoecaria agallocha leaves against Aeromonas hydrophila isolated from infected marine ornamental fish, Amphiprion sebae. Infected ten fish samples were collected from the marine ornamental fish hatchery and the bacteria was isolated and identified as Aeromonas hydrophila. Antibiotic resistance characterization was performed with commercial antibiotics. In vitro antibacterial activity of methanol and acetone extract of Excoecaria agallocha and their minimum inhibitory concentration (MIC) were assessed. Healthy Amphiprion sebae when challenged with the Aeromonas hydrophila, resulted in the development of clinical signs, those observed in the hatchery. The methanol extract of Excoecaria agallocha leaf was administrated with live A. hydrophila, decreased the percentage of mortality in the experimental groups. The phytochemical screening of the extract showed the presence of alkaloids, phytosterols, fixed oils and fats, tannins, phenolic compounds, proteins, free amino acids, gums, mucilage, flavonoids and lignin. The results revealed that the Excoecaria agallocha leaves have the potential to control the Aeromonas hydrophila diseases in marine ornamental fish, Amphiprion sebae

Chiral separation of carboprost isomers by normal phase LC using amylose chiral stationary phase

LC separation of carboprost diastereomers in bulk drug was developed and validated using normal-phase amylose stationary phase Chiralpak AD-H. The effect of the organic modifiers, namely 2-propanol and ethanol in the mobile phase was optimized in order to obtain the best separation. The retention time of (R)-carboprost and (S)-carboprost were 15.3 and 17.1 min, respectively. Calibration curves were linear over the range of 0.2–1.0%, with the regression coefficient (R 2) of 0.9997. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.07 and 0.2%, respectively. The method was accurate, precise and suitable to use for the purpose of controlling unwanted (R)-isomer in the carboprost active pharmaceutical ingredient. This method can be successfully applied to the analysis of chiral purity of carboprost in pharmaceutical bulk drug samples

Development and Validation of Chiral Hplc Method for Quantitation of Enantiomer in Rosuvastatin Calcium

A new, simple, precise, rapid and accurate normal phase enantioselective high performance liquid chromatographic method was developed for enantiomeric resolution of Rosuvastatin, which is used for the treatment of hypercholesterolemia. This is a fourth highest selling drug in the United States, accounting approximately $5.2 billion in the year of 2013. The enantiomer of Rosuvastatin and lactone impurity of Rosuvastatin were resolved on a CHIRALPAK IB (250 x 4.6mm, 5?m) column using a simple mobile phase system containing nheptane, 2-propanol and trifluoroaceticacid (85:15:01v/v). The resolution between Rosuvastatin and enantiomer, Rosuvastatin and lactone impurity was good with resolution factors more than 2.0 and 4.0, respectively. The effect of organic modifier, namely 2-propanol in the mobile phase was optimized in order to obtain the best separation.The Limit of Detection and Limit of Quantitation of enantiomer were found to be 0.07?g/mL and 0.2?g/mL, respectively, for 10?L injection volume. The sample solution and mobile phase were stable for at least 48 hours. The proposed reproducible and accurate method can be useful for quantification of enantiomer of Rosuvastatin in the bulk drug substance.&nbsp