201 research outputs found

    FisHook -- An Optimized Approach to Marine Specie Classification using MobileNetV2

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    Marine ecosystems are vital for the planet's health, but human activities such as climate change, pollution, and overfishing pose a constant threat to marine species. Accurate classification and monitoring of these species can aid in understanding their distribution, population dynamics, and the impact of human activities on them. However, classifying marine species can be challenging due to their vast diversity and the complex underwater environment. With advancements in computer performance and GPU-based computing, deep-learning algorithms can now efficiently classify marine species, making it easier to monitor and manage marine ecosystems. In this paper, we propose an optimization to the MobileNetV2 model to achieve a 99.83% average validation accuracy by highlighting specific guidelines for creating a dataset and augmenting marine species images. This transfer learning algorithm can be deployed successfully on a mobile application for on-site classification at fisheries

    Characterizing the normal proteome of human ciliary body

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    BACKGROUND: The ciliary body is the circumferential muscular tissue located just behind the iris in the anterior chamber of the eye. It plays a pivotal role in the production of aqueous humor, maintenance of the lens zonules and accommodation by changing the shape of the crystalline lens. The ciliary body is the major target of drugs against glaucoma as its inhibition leads to a drop in intraocular pressure. A molecular study of the ciliary body could provide a better understanding about the pathophysiological processes that occur in glaucoma. Thus far, no large-scale proteomic investigation has been reported for the human ciliary body. RESULTS: In this study, we have carried out an in-depth LC-MS/MS-based proteomic analysis of normal human ciliary body and have identified 2,815 proteins. We identified a number of proteins that were previously not described in the ciliary body including importin 5 (IPO5), atlastin-2 (ATL2), B-cell receptor associated protein 29 (BCAP29), basigin (BSG), calpain-1 (CAPN1), copine 6 (CPNE6), fibulin 1 (FBLN1) and galectin 1 (LGALS1). We compared the plasma proteome with the ciliary body proteome and found that the large majority of proteins in the ciliary body were also detectable in the plasma while 896 proteins were unique to the ciliary body. We also classified proteins using pathway enrichment analysis and found most of proteins associated with ubiquitin pathway, EIF2 signaling, glycolysis and gluconeogenesis. CONCLUSIONS: More than 95% of the identified proteins have not been previously described in the ciliary body proteome. This is the largest catalogue of proteins reported thus far in the ciliary body that should provide new insights into our understanding of the factors involved in maintaining the secretion of aqueous humor. The identification of these proteins will aid in understanding various eye diseases of the anterior segment such as glaucoma and presbyopia

    Deposition fluxes of chemical components of fog water at a rural site in North East India

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    The north-eastern part of India has not been investigated for atmospheric deposition studies so far. In this study, deposition fluxes on an artificial surface of chemical components due to fog have been calculated during winter from December 2002 to January 2003 at Jorhat, a rural site in north-east India. As the land in this area is covered with vegetation, the site is representative of rural characteristics in this region. The average pH of fog water is 5.6. Among chemical components, NH+ 4 was observed to be dominating ion. Soil pH in this region is acidic (4.9). Unlike other parts of the country, chemical analysis of soil in this region revealed that influence of suspended soil dust on fog deposition was insignificant. A comparison of fluxes of fog with rain water during December–January months showed that deposition fluxes due to rain water were higher by almost one order of magnitude on an artificial surface but on natural surfaces, the fluxes may be comparabl

    Major and minor fisheries harbours of India. 8. Fisherries harbours along the coasts of North Andhra Pradesh, Orissa and West Bengal

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    The present account gives information about the three major and five minor fisheries harbours along the coasts of north Andhra Pradesh, Orissa and West Benga

    Prospective nutritional, therapeutic, and dietary benefits of camel milk making it a viable option for human consumption: Current state of scientific knowledge

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    For over five thousand years, people in Asia and Africa have known about the health benefits of camel milk. Thus, it is used not only as a food source but also as a medicine. The similarities between camel milk and human milk have been scientifically proven. Camel milk is unique among ruminant milk because it is high in vitamins C and E and low in sugar and cholesterol. Still, it contains a wide variety of beneficial minerals (including sodium, potassium, iron, copper, zinc, and magnesium), besides being rich in several nutrients, including monounsaturated and polyunsaturated fatty acids, serum albumin, lactoferrin, immunoglobulins, lysozyme and the hormone insulin. Because of these components, many medical professionals now recommend camel milk as a treatment for various human ailments. It has been demonstrated to be effective in treating gastrointestinal issues, Type 1 diabetes, and food allergies. As a bonus, camel milk has been utilized to cure autism, lower cholesterol, prevent psoriasis, heal inflammation, aid tuberculosis patients, boost the body's natural defences, and impede the spread of cancer cells. Those who have problems digesting lactose may still be able to tolerate it. Conversely, camel milk can also help reduce an excessively high bilirubin, globulin, and granulocyte count. Drinking camel milk does not affect the erythrocyte sedimentation rate, hemoglobin concentration, and leukocyte count. The proteins in camel milk have an adequate ratio of critical amino acids. Immunoglobulins, which fight disease, are contained inside, and their small size allows antigens to penetrate and boosts the immune system's efficacy. This article highlights the health benefits and medicinal uses of camel milk

    Beneficial impacts of biochar as a potential feed additive in animal husbandry

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    In the last decade, biochar production and use have grown in popularity. Biochar is comparable to charcoal and activated charcoal because it is a pyrogenic carbonaceous matter made by pyrolyzing organic carbon-rich materials. There is a lack of research into the effects of adding biochar to animal feed. Based on the reviewed literature, including its impact on the adsorption of toxins, blood biochemistry, feed conversion rate, digestion, meat quality, and greenhouse gas emissions, adding biochar to the diet of farm animals is a good idea. This study compiles the most important research on biochar's potential as a supplement to the diets of ruminants (including cows and goats), swine, poultry, and aquatic organisms like fish. Biochar supplementation improves animal growth, haematological profiles, meat, milk and egg yield, resistance to illnesses (especially gut pathogenic bacteria), and reduced ruminant methane emission. Biochar's strong sorption capacity also helps efficiently remove contaminants and poisons from the animals' bodies and the farm surroundings where they are raised. Animal farmers are predicted to make greater use of biochar in the future. Biochar could potentially be of value in the healthcare and human health fields; hence research into this area is encouraged. The present review highlights the potential benefits of biochar as an additive to animal feed and demonstrates how, when combined with other environmentally friendly practices, biochar feeding can extend the longevity of animal husbandry

    Elevated Oestrogen Receptor Splice Variant ERαΔ5 Expression in Tumour-adjacent Hormone-responsive Tissue

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    Susceptibility to prostate or endometrial cancer is linked with obesity, a state of oestrogen excess. Oestrogen receptor (ER) splice variants may be responsible for the tissue-level of ER activity. Such micro-environmental regulation may modulate cancer initiation and/or progression mechanisms. Real-time reverse transcriptase (RT) polymerase chain reaction (PCR) was used to quantitatively assess the levels of four ER splice variants (ERαΔ3, ERαΔ5, ERβ2 and ERβ5), plus the full-length parent isoforms ERα and ERβ1, in high-risk [tumour-adjacent prostate (n = 10) or endometrial cancer (n = 9)] vs. low-risk [benign prostate (n = 12) or endometrium (n = 9)], as well as a comparison of UK (n = 12) vs. Indian (n = 15) benign prostate. All three tissue groups expressed the ER splice variants at similar levels, apart from ERαΔ5. This splice variant was markedly raised in all of the tumour-adjacent prostate samples compared to benign tissues. Immunofluorescence analysis for ERβ2 in prostate tissue demonstrated that such splice variants are present in comparable, if not greater, amounts as the parent full-length isoform. This small pilot study demonstrates the ubiquitous nature of ER splice variants in these tissue sites and suggests that ERαΔ5 may be involved in progression of prostate adenocarcinoma

    Dynamics of Hot QCD Matter -- Current Status and Developments

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    The discovery and characterization of hot and dense QCD matter, known as Quark Gluon Plasma (QGP), remains the most international collaborative effort and synergy between theorists and experimentalists in modern nuclear physics to date. The experimentalists around the world not only collect an unprecedented amount of data in heavy-ion collisions, at Relativistic Heavy Ion Collider (RHIC), at Brookhaven National Laboratory (BNL) in New York, USA, and the Large Hadron Collider (LHC), at CERN in Geneva, Switzerland but also analyze these data to unravel the mystery of this new phase of matter that filled a few microseconds old universe, just after the Big Bang. In the meantime, advancements in theoretical works and computing capability extend our wisdom about the hot-dense QCD matter and its dynamics through mathematical equations. The exchange of ideas between experimentalists and theoreticians is crucial for the progress of our knowledge. The motivation of this first conference named "HOT QCD Matter 2022" is to bring the community together to have a discourse on this topic. In this article, there are 36 sections discussing various topics in the field of relativistic heavy-ion collisions and related phenomena that cover a snapshot of the current experimental observations and theoretical progress. This article begins with the theoretical overview of relativistic spin-hydrodynamics in the presence of the external magnetic field, followed by the Lattice QCD results on heavy quarks in QGP, and finally, it ends with an overview of experiment results.Comment: Compilation of the contributions (148 pages) as presented in the `Hot QCD Matter 2022 conference', held from May 12 to 14, 2022, jointly organized by IIT Goa & Goa University, Goa, Indi

    Integrating transcriptomic and proteomic data for accurate assembly and annotation of genomes

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    © 2017 Wong et al.; Published by Cold Spring Harbor Laboratory Press. Complementing genome sequence with deep transcriptome and proteome data could enable more accurate assembly and annotation of newly sequenced genomes. Here, we provide a proof-of-concept of an integrated approach for analysis of the genome and proteome of Anopheles stephensi, which is one of the most important vectors of the malaria parasite. To achieve broad coverage of genes, we carried out transcriptome sequencing and deep proteome profiling of multiple anatomically distinct sites. Based on transcriptomic data alone, we identified and corrected 535 events of incomplete genome assembly involving 1196 scaffolds and 868 protein-coding gene models. This proteogenomic approach enabled us to add 365 genes that were missed during genome annotation and identify 917 gene correction events through discovery of 151 novel exons, 297 protein extensions, 231 exon extensions, 192 novel protein start sites, 19 novel translational frames, 28 events of joining of exons, and 76 events of joining of adjacent genes as a single gene. Incorporation of proteomic evidence allowed us to change the designation of more than 87 predicted noncoding RNAs to conventional mRNAs coded by protein-coding genes. Importantly, extension of the newly corrected genome assemblies and gene models to 15 other newly assembled Anopheline genomes led to the discovery of a large number of apparent discrepancies in assembly and annotation of these genomes. Our data provide a framework for how future genome sequencing efforts should incorporate transcriptomic and proteomic analysis in combination with simultaneous manual curation to achieve near complete assembly and accurate annotation of genomes
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