Archive - A Data Management Program

To meet funding agency requirements, a portable data management solution is presented for small research groups. The database created is simple, searchable, robust, and can reside across multiple hard drives. Employing a standard metadata schema for all data, the database ensures a high level of standardization, findability, and organization. The software is written in Perl, runs on UNIX, and presents a web-based user interface. It uses a fast, portable log-in scheme, making it easy to export to other locations. As research continues to move towards more open data sharing and reproducibility, this database solution is agile enough to accommodate external participants, while satisfying the unique needs of the internal research group

Layer-Specific Noradrenergic Modulation of Inhibition in Cortical Layer II/III

Norepinephrine (NE) is released in the neocortex after activation of the locus coeruleus of the brain stem in response to novel, salient, or fight-or-flight stimuli. The role of adrenergic modulation in sensory cortices is not completely understood. We investigated the possibility that NE modifies the balance of inhibition acting on 2 different γ-aminobutyric acid (GABA)ergic pathways. Using patch-clamp recordings, we found that the application of NE induces an α1 adrenergic receptor–mediated decrease of the amplitude of inhibitory postsynaptic currents (IPSCs) evoked by stimulation of layer I (LI-eIPSCs) and a β and α2 receptor–mediated increase in the amplitude of IPSCs evoked by stimulation of layer II/III (LII/III-eIPSCs). Analysis of minimal stimulation IPSCs, IPSC kinetics, and sensitivity to the GABAA receptor subunit–selective enhancer zolpidem corroborated the functional difference between LI- and LII/III-eIPSCs, suggestive of a distal versus somatic origin of LI- and LII/III-eIPSCs, respectively. These findings suggest that NE shifts the balance between distal and somatic inhibition to the advantage of the latter. We speculate that such shift modifies the balance of sensory-specific and emotional information in the integration of neural input to the upper layers of the auditory cortex