62 research outputs found

    Stokes settling and particle-laden plumes: implications for deep-sea mining and volcanic eruption plumes.

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    Turbulent buoyant plumes moving through density stratified environments transport large volumes of fluid vertically. Eventually, the fluid reaches its neutral buoyancy level at which it intrudes into the environment. For single-phase plume, the well-known theory of Morton, Taylor and Turner (Morton BR, Taylor GI, Turner JS. 1956 Turbulent gravitational convection from maintained and instantaneous sources. Proc. R. Soc. A 234, 1-23. (doi:10.1098/rspa.1956.0011)) describes the height of the intrusion with great accuracy. However, in multiphase plumes, such as descending particle plumes formed from the surface vessel during deep-sea mining operations, or ascending volcanic plumes, consisting of hot gas and dense ash particles, the sedimentation of particles can change the buoyancy of the fluid very significantly. Even if the plume speed far exceeds the sedimentation speed, the ultimate intrusion height of the fluid may be significantly affected by particle sedimentation. We explore this process, illustrating the phenomena with a series of analogue experiments and some simple modelling, and we discuss the applications in helping to quantify some environmental impacts of deep-sea mining and in helping to assess the eruption conditions leading to the formation of large laterally spreading ash clouds in the atmosphere. This article is part of the theme issue 'Stokes at 200 (part 2)'

    HcRed, a Genetically Encoded Fluorescent Binary Cross-Linking Agent for Cross-Linking of Mitochondrial ATP Synthase in Saccharomyces cerevisiae

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    Genetically encoded fluorescent cross-linking agents represent powerful tools useful both for visualising and modulating protein interactions in living cells. The far-red fluorescent protein HcRed, which is fluorescent only in a dimer form, can be used to promote the homo-dimerisation of target proteins, and thereby yield useful information about biological processes. We have in yeast cells expressed HcRed fused to a subunit of mitochondrial ATP synthase (mtATPase). This resulted in cross-linking of the large multi-subunit mtATPase complex within the inner-membrane of the mitochondrion. Fluorescence microscopy revealed aberrant mitochondrial morphology, and mtATPase complexes isolated from mitochondria were recovered as fluorescent dimers under conditions where complexes from control mitochondria were recovered as monomers. When viewed by electron microscopy normal cristae were absent from mitochondria in cells in which mATPase complexes were cross-linked. mtATPase dimers are believed to be the building blocks that are assembled into supramolecular mtATPase ribbons that promote the formation of mitochondrial cristae. We propose that HcRed cross-links mATPase complexes in the mitochondrial membrane hindering the normal assembly/disassembly of the supramolecular forms of mtATPase

    Search for Resonance Decays to Lepton+jet at DESY HERA and Limits on Leptoquarks

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    A search for narrow-width resonances that decay into electron+jet or neutrino+jet has been performed with the ZEUS detector at the DESY ep collider HERA operating at center-of-mass energies of 300 and 318 GeV. An integrated e+p luminosity of 114.8 pb-1 and e-p luminosity of 16.7 pb-1 were used. No evidence for any resonance was found. Limits were derived on the Yukawa coupling λ as a function of the mass of a hypothetical resonance that has arbitrary decay branching ratios into eq or vq. These limits also apply to squarks predicted by R-parity-violating supersymmetry. Limits for the production of leptoquarks described by the Buchmüller-Rückl-Wyler model were also derived for masses up to 400 GeV. For λ = 0.1, leptoquark masses up to 290 GeV are excluded

    Under pressure: turbulent plumes in a uniform crossflow

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    Direct numerical simulation is used to investigate the integral behaviour of buoyant plumes subjected to a uniform crossflow that are infinitely lazy at the source. Neither a plume trajectory defined by the centre of mass of the plume zc nor a trajectory defined by the central streamline zU is aligned with the average streamlines inside the plume. Both zc and zU are shown to correlate with field lines of the total buoyancy flux, which implies that a model for the vertical turbulent buoyancy flux is required to faithfully predict the plume angle. A study of the volume conservation equation shows that entrainment due to incorporation of ambient fluid with non-zero velocity due to the increase in the surface area (the Leibniz term) is the dominant entrainment mechanism in strong crossflows. The data indicate that pressure differences between the top and bottom of the plume play a leading role in the evolution of the horizontal and vertical momentum balances and are crucial for appropriately modelling plume rise. By direct parameterisation of the vertical buoyancy flux, the entrainment and the pressure, an integral plume model is developed which is in good agreement with the simulations for sufficiently strong crossflow. A perturbation expansion shows that the current model is an intermediate-range model valid for downstream distances up to 100b–1000b, where b is the buoyancy length scale based on the flow speed and plume buoyancy flux

    Results of the eruptive column model inter-comparison study

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    This study compares and evaluates one-dimensional (1D) and three-dimensional (3D) numerical models of volcanic eruption columns in a set of different inter-comparison exercises. The exercises were designed as a blind test in which a set of common input parameters was given for two reference eruptions, representing a strong and a weak eruption column under different meteorological conditions. Comparing the results of the different models allows us to evaluate their capabilities and target areas for future improvement. Despite their different formulations, the 1D and 3D models provide reasonably consistent predictions of some of the key global descriptors of the volcanic plumes. Variability in plume height, estimated from the standard deviation of model predictions, is within ~20% for the weak plume and ~10% for the strong plume. Predictions of neutral buoyancy level are also in reasonably good agreement among the different models, with a standard deviation ranging from 9 to 19% (the latter for the weak plume in a windy atmosphere). Overall, these discrepancies are in the range of observational uncertainty of column height. However, there are important differences amongst models in terms of local properties along the plume axis, particularly for the strong plume. Our analysis suggests that the simplified treatment of entrainment in 1D models is adequate to resolve the general behaviour of the weak plume. However, it is inadequate to capture complex features of the strong plume, such as large vortices, partial column collapse, or gravitational fountaining that strongly enhance entrainment in the lower atmosphere. We conclude that there is a need to more accurately quantify entrainment rates, improve the representation of plume radius, and incorporate the effects of column instability in future versions of 1D volcanic plume models.AC was partially supported by a grant of the International Research Promotion Office Earthquake Research Institute, the University of Tokyo. AC, GM, AN, MdMV, TEO and MC were partially supported by the EU-funded project MEDiterranean SUpersite Volcanoes (MEDSUV) (grant n. 308665). AVE acknowledges NSF Postdoctoral Fellowship EAR1250029 and a USGS Mendenhall Fellowship. MIB was supported partially by NSF-IDR and AFOSR. AJH, MJW, and JCP were partially supported by the NERC-funded project Vanaheim (grant no. NE/I01554X/1) and the EU-funded project FutureVolc (grant no. 308377). FG, GC, ST, and EK were partially supported by INSU-CNRS. We wish to thank T. ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT 43 Koyaguchi, S. Solovitz, and an anonymous reviewer for constructive suggestions that improved the quality of the manuscript.This is the author accepted manuscript. The final version is available from Elsevier via https://doi.org/10.1016/j.jvolgeores.2016.01.01
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