Complementary analytical methods for the phytochemical investigation of ‘Jardin de Granville’, a rose dedicated to cosmetics

Abstract‘Jardin de Granville’ is a new hybrid rose variety dedicated to cosmetic applications. To perform an exhaustive molecular investigation of the non-volatile secondary metabolites in this cultivar, a global approach was developed, combining four chromatographic techniques (HPTLC, HPLC-DAD-ELSD, UHPLC-HRMS and GC–MS). This approach afforded an on-line phytochemical fingerprinting of four plant organs of ‘Jardin de Granville’. Despite the wide diversity of molecular families and the pronounced differences in polarity between the molecules, this analytical strategy enabled an overview of the molecular composition of each sample to be rapidly obtained by HPTLC and HPLC and the molecular content to be correctly identified thanks to coupling with mass spectrometry. Polyphenols were identified in the EtOH/H2O extracts; triterpenes, chlorophyll derivatives and lipids were characterized in the EtOAc extracts, and the fatty acids squalene, α-tocopherol and β-sitosterol were highlighted in the heptane extracts

Chélation de l'aluminium (III) et fluorescence (de la caractérisation à l'application)

CACHAN-ENS (940162301) / SudocSudocFranceF

Chêne sessile et chêne pédonculé, une nouvelle approche pour les reconnaître ?

Le bois de Chêne jusqu’au XIXème siècle, était la principale essence utilisée en Europe dans la construction navale et la construction des charpentes. C'est aujourd'hui encore une essence très prisée, notamment pour la fabrication des tonneaux dans lesquels les vins et spiritueux sont vieillis.Le fût de chêne n’est pas un simple contenant, il transmet de nombreux composés aux vins qu’il contient et en modifie les propriétés sensorielles. Aujourd’hui, il n’est plus employé pour le transport des boissons mais est devenu un outil essentiel pour l’élevage des vins et spiritueux qui bénéficient d’une complexité et d’une originalité qui leur sont propres, grâce à l’élevage sous-bois

Water-Based Microwave-Assisted Extraction of Pigments from Madder Optimized by a Box–Behnken Design

Water is one of the greenest and most accessible solvents. To harness its potential, the water-based microwave-assisted extraction (MAE) of pigments from madder was optimized by the response surface methodology (RSM) using a Box–Behnken experimental design. The extract that presented both the highest extraction yield and the most intense color was obtained after a 30-s cycle at 1000 W using 100 mg of madder for 20 mL of water. This water-based MAE was more efficient than Soxhlet extraction and proved comparable to hydroalcoholic MAE. The optimized extract was further characterized using UHPLC-HRMS/MS to identify its main compounds. Glycosylated flavonoids and anthraquinones were putatively identified, as well as free anthraquinones, generally found in madder. The microwave-assisted extraction extended the range of polarity of the extracted compounds, making the water more versatile

Optimization of supercritical fluid extraction of polar flavonoids from Robinia pseudoacacia L. heartwood

The black locust, known as an invasive species, represent many advantages, mainly due to its rapid growth rate and its richness with interesting flavonoids. In this study, supercritical fluid extraction of the two main flavonoids dihydrorobinetin and robinetin, was optimized using a three level Box-Behnken response surface design. The conditions investigated to influence the extraction yield of these flavonoids were temperature (40–80 °C), pressure (10–20 MPa) and modifier’s percentage EtOH:H2O 80:20 (10 %−30 %). The response was quantified using an UHPLC-DAD optimized chromatographic method. The results showed the influence of the extraction conditions on the yield of the molecules of interest. The data were fitted into a second-order polynomial equation, the multiple regression analysis showed a high determination coefficient value (R2) of 0.93. The optimal extraction conditions were 10 MPa, 80 °C and extraction phase composition of CO2:EtOH:H2O 80:16:4 for 30 min. These conditions allowed for the recovery 49.2 mg of flavonoids for 1 g of dried wood powder

Valbiodiv.Centre : Protection, Innovation et Valorisation de la flore régionale.

National audienceThe linear economic model is reaching its limits. Our Centre-Val-de-Loire region has so much species of plants, and some of them are not well-known.The Valbiodiv.Centre group is composed of different local economic actors who work on plants. Using their own skills and means, they protect, innovate, and promote this biodiversity. 20 species were investigated in terms of many different fields of knowledge. In the same time, there was a monitoring of the laws, relating to the Nagoya protocol.The group will choose some plants to investigate their phytochemistry and their biological properties, to improve our knowledge.Devant un modèle économique linéaire à bout de souffle, et une richesse botanique avérée et peu connue de notre région Centre-Val-de-Loire, le collectif Valbiodiv.Centre, constitué de différents acteurs locaux dont le cœur de métier est la plante, déploie des combinaisons de compétences et de moyens divers propres pour protéger, innover, et valoriser cette biodiversité végétale. Vingt espèces sont soumises à une étude bibliographique pluridisciplinaire, en parallèle de la veille de la traduction en lois du Protocole de Nagoya. Quelques-unes seront priorisées par le groupe afin de mener des recherches phytochimiques et biologiques qui engendreront les savoirs de demain

Oak Species Quercus robur L. and Quercus petraea Liebl. Identification Based on UHPLC-HRMS/MS Molecular Networks

International audienceTwo species of oak are dominant in French forests: pedunculate oak (Quercus robur L.) and sessile oak (Quercus petraea Liebl.). Their differentiation is not straightforward but is essential to better understand their respective molecular content in order to better valorize them. Thus, to improve oak species identification, an untargeted UHPLC-HRMS/MS method associated with a two-step data treatment was developed to analyze a wide range of specialized metabolites enabling the comparison of both species of oak extracts. Pooled extracts from sessile and pedunculate oaks, composed of extracts from several trees of pure species from various origins, were compared using first the Venn diagram, as a quick way to get an initial idea of how close the extracts are, and then using a molecular network to visualize, on the one hand, the ions shared between the two species and, on the other hand, the compounds specific to one species. The molecular network showed that the two species shared common clusters mainly representative of tannins derivatives and that each species has specific molecules with similar fragmentation patterns, associated in specific clusters. This methodology was then applied to compare these two pooled extracts to unknown individuals in order to determine the species. The Venn diagram allowed for the quick presumption of the species of the individual and then the species could be assigned more precisely with the molecular network, at the level of specific clusters. This method, developed for the first time, has several interests. First, it makes it possible to discriminate the species and to correctly assign the species of unknown samples. Moreover, it gave an overview of the metabolite composition of each sample to better target oak tree utilization and valorization

TLC-MALDI-TOF-MS-based identification of flavonoid compounds using an inorganic matrix

International audienc

Characterization of Corn Silk Extract Using HPLC/HRMS/MS Analyses and Bioinformatic Data Processing

In addition to having different biological activities of interest, corn silks play a role in the defense of plants. While benzoxamines and flavonoids have already been identified as molecules of plant defense and growth mechanisms, knowledge on the phytochemical composition of corn silk is lacking. Such knowledge would make it possible to better select the most effective varieties to improve resistance or bioactive properties. In this article, an approach was implemented to map a corn silk extract in two complementary ways. The first one involved working with UHPLC/HRMS data and Kendrick and van Krevelen plots to highlight a homologous series of compounds, such as lipids from 17 to 23 carbons, monoglycosylated flavonoids from 21 to 24 carbons, diglycosylated flavonoids of 26 to 28 carbons and organic acids of 14 to 19 carbons. The second way was to analyze the sample in UHPLC/HRMS2 and to plot mass spectral similarity networks with the GNPS platform and Cytoscape software to refine identification. By combining the information obtained, we were able to propose an identification for 104 detected molecules, including 7 nitrogenous, 28 lipidic and 67 phenolic compounds, leading to the first detailed phytochemical analysis of corn silk extract