Impact of Initial Spacing on Plantation Black Spruce Lumber Grade Yield, Bending Properties, and MSR Yield

For decades, initial spacing of 2 m X 2 m has been used for black spruce (Picea mariana) reforestation in eastern Canada. In recent years, however, wider spacings for black spruce are being advocated to reduce establishment costs and accelerate tree growth. Wider spacings will affect not only return on investment but also the quality of products from the plantations, both of which are critical to the success of reforestation programs. As part of a multidisciplinary project, this study evaluated and quantified the impact of initial spacing on lumber grade yield, bending properties, and MSR yield in this species. Furthermore, visual grades of the plantation-grown lumber were compared for their bending properties and their compliance to the current grade requirements for bending stiffness. A total of 139 sample trees were collected from 4 different spacings (3,086, 2,500, 2,066, 1,372 trees/ha) in a 48-year-old initial spacing trial, and 849 pieces of 2-in.-thick lumber from the 4 spacings were graded visually and tested for bending strength and stiffness.With decreasing initial stand density from 3,086 to 2,066 trees/ha, branch diameter showed a steady increase. However, the 3 higher stand densities (3,086, 2,500, and 2,066 trees/ha) had a comparable Select Structural (SS) grade yield thanks to the relatively small branches in this species. Lumber strength and stiffness in those 3 spacings were also quite comparable. When the initial stand density was further reduced to 1,372 tree/ha, however, a remarkable decrease in the SS grade yield due to knots occurred, and lumber strength and stiffness also decreased significantly. The real concern occurred when the plantation-grown lumber was compared to that from natural stands currently being processed in eastern Canada. On average, the plantation-grown black spruce lumber stiffness was 28.9% lower than that of lumber from the natural stands. As a result, a high percentage of the plantation-grown lumber did not meet the bending design values. However, the percentage of the compliance to the design values tended to increase with increasing initial stand density. This article discusses the possible causes for the significantly lower bending properties of the plantation-grown lumber, and potential solutions for increasing lumber properties and the percentage of the compliance

Multi-scale spectrally resolved quantitative fluorescence imaging system: Towards neurosurgical guidance in glioma resection

In glioma resection surgery, the detection of tumour is often guided by using intraoperative fluorescence imaging notably with 5-ALA-PpIX, providing fluorescent contrast between normal brain tissue and the gliomas tissue to achieve improved tumour delineation and prolonged patient survival compared with the conventional white-light guided resection. However, the commercially available fluorescence imaging system relies on surgeon’s eyes to visualise and distinguish the fluorescence signals, which unfortunately makes the resection subjective. In this study, we developed a novel multi-scale spectrally-resolved fluorescence imaging system and a computational model for quantification of PpIX concentration. The system consisted of a wide-field spectrally-resolved quantitative imaging device and a fluorescence endomicroscopic imaging system enabling optical biopsy. Ex vivo animal tissue experiments as well as human tumour sample studies demonstrated that the system was capable of specifically detecting the PpIX fluorescent signal and estimate the true concentration of PpIX in brain specimen

Soft optically-tuneable fluorescence phantoms based on gel wax and quantum dots: a tissue surrogate for fluorescence imaging validation

Fluorescence-guided brain tumour resection, notably using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) for high-grade gliomas, has been demonstrated to provide better tissue differentiation, thereby improving patient outcomes when compared to white-light guidance. Novel fluorescence imaging devices aiming to increase detection specificity and sensitivity and targeting applications beyond high-grade gliomas are typically assessed by measurements using tissue-mimicking optical phantoms. The field currently lacks adequate phantoms with well-characterised tuneable optical properties. In this study, we developed soft tissue-mimicking fluorescence phantoms (TMFP) highly suitable for this purpose. We investigated: 1) the ability to independently tune optical and fluorescent properties; 2) the stability of the fluorescence signal over time; and 3) the potential of the proposed phantoms for imaging device validation. The TMFP is based on gel-wax which is an optically transparent mineral-oil based soft material. We embedded TiO2 as scattering material, carbon black oil-paint as background absorber, and CdTe Quantum Dots (QDs) as fluorophore because of its similar fluorescence spectrum to PpIX. Scattering and absorption properties were measured by a spectrophotometer, while the fluorescence was assessed by a wide-field fluorescence imaging system (WFFI) and a spectrometer. We demonstrated that: 1) the addition of QDs didn’t alter the phantom’s scattering which was only defined by the concentration of TiO2, whereas its absorption was defined by both QDs and colour oil paint; 2) the measured fluorescence intensity was linearlyproportional to the concentration of QDs; 3) the fluorescence intensity was stable over time (up to eight months); and 4) the fluorescence signal measured by the WFFI were strongly correlated to spectrometer measurements

A Blow-Up Criterion for Classical Solutions to the Compressible Navier-Stokes Equations

In this paper, we obtain a blow up criterion for classical solutions to the 3-D compressible Naiver-Stokes equations just in terms of the gradient of the velocity, similar to the Beal-Kato-Majda criterion for the ideal incompressible flow. In addition, initial vacuum is allowed in our case.Comment: 25 page

Mycobacterium tuberculosis phagosome

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75241/1/j.1365-2958.1999.01279.x.pd

Real-Time Ultrasonic Tracking of an Intraoperative Needle Tip with Integrated Fibre-Optic Hydrophone

We have developed a real-time ultrasound needle tracking system based on a fibre-optic hydrophone integrated into an intraoperative needle. The system is intended to track the location of a needle tip during minimally invasive surgical procedures that rely on handheld ultrasound guidance. During these procedures, clear visibility of the needle tip is essential to reach the procedure target and avoid adverse events due to erroneous needle placement. The ultrasound emitted by the handheld probe is detected by the hydrophone and used to draw a cursor marking the position of the needle tip within the B-mode ultrasound image. The tracking accuracy was assessed by comparison to manual labelling of the location of the needle tip within the ultrasound images and found to be ±2.2 mm

Real-Time, Video-Rate and Depth-Resolved Imaging of Radio-Frequency Ablation Using All-Optical Ultrasound

Radio-frequency (RF)ablation is an effective minimally invasive treatment for atrial fibrillation, where RF fields are delivered by a catheter within the heart. In current clinical practice, this procedure is performed without real-time feedback about the viability of tissue beneath the ablation lesion. As a result, it can be challenging to perform complete ablation of the target tissue whilst avoiding damage to adjacent structures. Recently, all-optical ultrasound imaging, where ultrasound is both generated and detected using light, has been demonstrated as a viable imaging modality exhibiting high image resolution, strong miniaturisation potential, and resilience to interfererence by electromagnetic (EM)fields from the RF ablation catheter. In this work, the suitability of all-optical ultrasound to monitoring RF ablation lesion delivery is investigated using a bench-top imaging system. This system is capable of real-time, video-rate 2D imaging at an image depth of 15 mm, a resolution of 100 μm, a dynamic range of 35 dB, and a sustained frame rate of 9 Hz. In this paper we present the first real-time, video-rate all-optical ultrasound images acquired during RF ablation, and demonstrate how the imaging system was able to clearly visualise the lesion growth at a contrast of up to 30 dB. In addition, we demonstrated how lesion growth in inhomogeneous tissue can progress in an erratic fashion. High-quality imaging during treatment will enable optimisation of the ablation parameters during treatment, and will minimise both treatment times and complication rates

Phosphoenolpyruvate carboxylase dentified as a key enzyme in erythrocytic Plasmodium falciparum carbon metabolism

Phospoenolpyruvate carboxylase (PEPC) is absent from humans but encoded in thePlasmodium falciparum genome, suggesting that PEPC has a parasite-specific function. To investigate its importance in P. falciparum, we generated a pepc null mutant (D10Δpepc), which was only achievable when malate, a reduction product of oxaloacetate, was added to the growth medium. D10Δpepc had a severe growth defect in vitro, which was partially reversed by addition of malate or fumarate, suggesting that pepc may be essential in vivo. Targeted metabolomics using 13C-U-D-glucose and 13C-bicarbonate showed that the conversion of glycolytically-derived PEP into malate, fumarate, aspartate and citrate was abolished in D10Δpepc and that pentose phosphate pathway metabolites and glycerol 3-phosphate were present at increased levels. In contrast, metabolism of the carbon skeleton of 13C,15N-U-glutamine was similar in both parasite lines, although the flux was lower in D10Δpepc; it also confirmed the operation of a complete forward TCA cycle in the wild type parasite. Overall, these data confirm the CO2 fixing activity of PEPC and suggest that it provides metabolites essential for TCA cycle anaplerosis and the maintenance of cytosolic and mitochondrial redox balance. Moreover, these findings imply that PEPC may be an exploitable target for future drug discovery

Wide-field spectrally resolved quantitative fluorescence imaging system: toward neurosurgical guidance in glioma resection

In high-grade glioma surgery, tumor resection is often guided by intraoperative fluorescence imaging. 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) provides fluorescent contrast between normal brain tissue and glioma tissue, thus achieving improved tumor delineation and prolonged patient survival compared with conventional white-light-guided resection. However, commercially available fluorescence imaging systems rely solely on visual assessment of fluorescence patterns by the surgeon, which makes the resection more subjective than necessary. We developed a wide-field spectrally resolved fluorescence imaging system utilizing a Generation II scientific CMOS camera and an improved computational model for the precise reconstruction of the PpIX concentration map. In our model, the tissue’s optical properties and illumination geometry, which distort the fluorescent emission spectra, are considered. We demonstrate that the CMOS-based system can detect low PpIX concentration at short camera exposure times, while providing high-pixel resolution wide-field images. We show that total variation regularization improves the contrast-to-noise ratio of the reconstructed quantitative concentration map by approximately twofold. Quantitative comparison between the estimated PpIX concentration and tumor histopathology was also investigated to further evaluate the system

Physical activity to improve cognition in older adults: can physical activity programs enriched with cognitive challenges enhance the effects? A systematic review and meta-analysis

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