Fermentation of animal components in strict carnivores: a comparative study with cheetah fecal inoculum

The natural diet of felids contains highly digestible animal tissues but also fractions resistant to small intestinal digestion, which enter the large intestine where they may be fermented by the resident microbial population. Little information exists on the microbial degradability of animal tissues in the large intestine of felids consuming a natural diet. This study aimed to rank animal substrates in their microbial degradability by means of an in vitro study using captive cheetahs fed a strict carnivorous diet as fecal donors. Fresh cheetah fecal samples were collected, pooled, and incubated with various raw animal substrates (chicken cartilage, collagen, glucosamine-chondroitin, glucosamine, rabbit bone, rabbit hair, and rabbit skin; 4 replicates per substrate) for cumulative gas production measurement in a batch culture technique. Negative (cellulose) and positive (casein and fructo-oligosaccharides; FOS) controls were incorporated in the study. Additionally, after 72 h of incubation, short-chain fatty acids (SCFA), including branched-chain fatty acids (BCFA), and ammonia concentrations were determined for each substrate. Glucosamine and glucosamine-chondroitin yielded the greatest OM cumulative gas volume (OMCV) among animal substrates (P < 0.05), whereas total SCFA production was greatest for collagen (P < 0.05). Collagen induced an acetate production comparable to FOS and a markedly high acetate-to-propionate ratio (8.41:1) compared to all other substrates (1.67:1 to 2.97:1)

Effect of diet type on serum and faecal concentration of S100/calgranulins in the captive cheetah

Gastrointestinal disease is omnipresent in captive cheetahs (Acinonyx jubatus), in contrast to its freeranging populations. The current study aimed to evaluate the effect of diet type (meat-only whole prey) on gastrointestinal health in captive cheetahs by measuring faecal and serum concentrations of S100/calgranulins. This paper reports faecal S100A12 and calprotectin concentrations in 12 captive cheetahs fed supplemented beef versus whole rabbit for one month in a cross-over design. Also, serum S100A12 and calprotectin concentrations were determined in four cheetahs fed whole rabbit and six cheetahs fed supplemented beef, and these were compared to the faecal concentrations of the respective marker proteins. Both the immunoassay for canine calprotectin and canine S100A12 were precise and reproducible for use with serum samples and faecal extracts. Whereas the assay for S100A12 was linear and accurate, an inconsistent linearity of the canine calprotectin assay was observed and could be indicative of an insufficient cross-reactivity of the specific antibody used for this assay. Serum concentrations of S100A12 and calprotectin were not altered by diet type, and were not correlated with the respective concentrations in faeces. Numerically (P=0.241) greater calprotectin concentrations and greater (P=0.041) faecal S100A12 concentrations were detected in cheetahs fed supplemented beef compared with whole rabbit. These findings demonstrate that whole prey feeding may decrease intestinal inflammation in the captive cheetah. Consequently, the relation between diet type and intestinal inflammatory conditions in the captive cheetah warrants further investigation

Body Image and Cosmesis after Percutaneous Transforaminal Endoscopic Discectomy versus Conventional Open Microdiscectomy for Sciatica

Study Design: Randomized controlled trial Objective: Percutaneous transforaminal endoscopic discectomy (PTED) was introduced as a less invasive procedure to treat sciatica. Even though the PTED has a small scar size, it is unknown if PTED also leads to better scar-related patient-reported outcomes. Therefore, we aimed to compare scar-related outcomes between patients undergoing PTED vs open microdiscectomy. Methods: Patients with at least 6 weeks of radiating leg pain were randomized in a 1:1 ratio to PTED or open microdiscectomy. Scar-related patient-reported outcomes were measured using the Body Image Score (BIS), Cosmesis Scale (CS) and a 0-10 numeric rating scale (NRS) on scar esthetic. Results: Of the 530 included patients, 286 patients underwent PTED and 244 underwent open microdiscectomy as allocated. At 12 months of follow-up, 95% of the patients had data available. At 12 months, the BIS was 6.2 ± 1.7 in the PTED-group and 6.6 ± 1.9 in the open microdiscectomy group (between-group difference.4, 95% CI.2 to.7). CS was 21.3 ± 3.0 in the PTED-group and 18.6 ± 3.4 in the open microdiscectomy group (between-group difference −2.7, 95% CI −3.1 to −2.3). Average NRS for scar esthetic was 9.2 ± 1.3 and 7.8 ± 1.6 in the PTED and open microdiscectomy groups, respectively (between-group difference −1.4, 95% CI −1.6 to −1.2) Conclusions: PTED leads to a higher self-rated scar esthetic as compared to open microdiscectomy, while self-reported body image seems to be comparable between both groups. Therefore, from an esthetic point, PTED seems to be the preferred technique to treat sciatica.</p

CLUSTER FORMATION BY FAST HEAVY ION IMPACT ON METAL ACETATES

No abstract availabl

Heavy gold cluster beams production and identification

NIM ACCIt is shown that beams of very heavy gold clusters can be produced by a liquid metal ion source (LMIS). An experimental method is described for defining the LMIS source and the Wien filter parameters that must be set to extract and select large Aun clusters. This method is based on the acceleration of the clusters to high energy (MeV) and on the measurement, after their passage through a thin foil, of their number of constituents and velocity. Only an average mass over charge value is obtained for a given set of source and Wien filter parameters. These parameters can then be used to select heavy Aun cluster beams for applications at low energy (keV) in mass spectrometry

Energy loss and angular distributions of gold cluster constituents

Heavy gold cluster beams are accelerated to high energy (hundreds of keV/atom) and break up when going through a thin foil. The energy and angular distributions of the constituents are then measured and very well reproduced by a SRIM code calculation, which takes into account atomic interactions only. These distributions do not depend on the number of constituents in the cluster and are found to be the same as those of single gold atoms at the same velocity, in the studied energy range

Steady nearly incompressible vector fields in 2D: chain rule and renormalization

Given bounded vector field $bcolon RR^d o RR^d$, scalar field $ucolon RR^d o RR$ and a smooth function $eta colon RR o RR$ we study the characterization of the distribution $div(eta(u)b)$ in terms of $div b$ and $div(u b)$. In the case of $BV$ vector fields $b$ (and under some further assumptions) such characterization was obtained by L. Ambrosio, C. De Lellis and J. Mal'y, up to an error term which is a measure concentrated on so-called emph{tangential set} of $b$. We answer some questions posed in their paper concerning the properties of this term. In particular we construct a nearly incompressible $BV$ vector field $b$ and a bounded function $u$ for which this term is nonzero. For steady nearly incompressible vector fields $b$ (and under some further assumptions) in case when $d=2$ we provide complete characterization of $div(eta(u) b)$ in terms of $div b$ and $div(u b)$. Our approach relies on the structure of level sets of Lipschitz functions on $RR^2$ obtained by G. Alberti, S. Bianchini and G. Crippa. Extending our technique we obtain new sufficient conditions when any bounded weak solution $u$ of $d_t u + b cdot abla u=0$ is emph{renormalized}, i.e. also solves $d_t eta(u) + b cdot abla eta(u)=0$ for any smooth function $eta colonRR o RR$. As a consequence we obtain new uniqueness result for this equation

Very large gold and silver sputtering yields induced by keV to MeV energy Aun_n clusters (n = 1-13)

CAS, BIA