Generalized Parameter Estimation-based Observers: Application to Power Systems and Chemical-Biological Reactors

In this paper we propose a new state observer design technique for nonlinear systems. It consists of an extension of the recently introduced parameter estimation-based observer, which is applicable for systems verifying a particular algebraic constraint. In contrast to the previous observer, the new one avoids the need of implementing an open loop integration that may stymie its practical application. We give two versions of this observer, one that ensures asymptotic convergence and the second one that achieves convergence in finite time. In both cases, the required excitation conditions are strictly weaker than the classical persistent of excitation assumption. It is shown that the proposed technique is applicable to the practically important examples of multimachine power systems and chemical-biological reactors.Comment: 13 pages, 8 figure

Characterization of mouse Dactylaplasia mutations: a model for human ectrodactyly SHFM3

SHFM3 is a limb malformation characterized by the absence of central digits. It has been shown that this condition is associated with tandem duplications of about 500 kb at 10q24. The Dactylaplasia mice display equivalent limb defects and the two corresponding alleles (Dac 1j and Dac 2j ) map in the region syntenic with the duplications in SHFM3. Dac 1j was shown to be associated with an insertion of an unspecified ETn-like mouse endogenous transposon upstream of the Fbxw4 gene. Dac 2j was also thought to be an insertion or a small inversion in intron 5 of Fbxw4, but the breakpoints and the exact molecular lesion have not yet been characterized. Here we report precise mapping and characterization of these alleles. We failed to identify any copy number differences within the SHFM3 orthologous genomic locus between Dac mutant and wild-type littermates, showing that the Dactylaplasia alleles are not associated with duplications of the region, in contrast with the described human SHFM3 cases. We further show that both Dac 1j and Dac 2j are caused by insertions of MusD retroelements that share 98% sequence identity. The differences between the nature of the human and mouse genomic abnormalities argue against models proposed so far that either envisioned SHFM3 as a local trisomy or Dac as a mutant allele of Fbxw4. Instead, both genetic conditions might lead to complex alterations of gene regulation mechanisms that would impair limb morphogenesis. Interestingly, the Dac 2j mutation occurs within a highly conserved element that may represent a regulatory sequence for a neighboring gen

Research on lymphedema by method of high-resolution multiphoton microscopy

In this paper results of iv-vivo measurements for healthy volunteers and people, with verified diagnosis of lymphedema, obtained with two-photon tomography with fluorescence lifetime imaging microscopy (FLIM) are presented. The papillary layer of the skin was analyzed at a depth of about 100 μm. The purpose of this study is to evaluate the lifetime of autofluorescence in the papillary dermis of healthy tissue and with the initial stage of lymphedema. In the course of the study, a small redistribution of autofluorescence lifetimes was observed for healthy volunteers and people with lymphedema disease. The research was carried out on the equipment of MPTflex (JenLab GmbH)