Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot

Cell signaling governs the basic functions of cells by molecular interactions that involve of many proteins. The abundance of signaling proteins can directly influence cellular responses to external signal, contributing to cellular heterogeneity. Absolute quantification of proteins is important for modeling and understanding the complex signaling network. Here, we introduce how to measure the amount of TGF-β signaling proteins using quantitative immunoblotting. In addition, we discuss how to convert the measurements of protein abundance to the quantities of absolute molecules per cell. This method is generally applicable to the absolute quantification of other proteins

Effect of water vapor on the spallation of thermal barrier coating systems during laboratory cyclic oxidation testing.

The effect of water and water vapor on the lifetime of Ni-based superalloy samples coated with a typical thermal barrier coating system—b-(Ni,Pt)Al bond coat and yttria stabilized zirconia (YSZ) top coat deposited by electron beam physical vapor deposition (EB-PVD) was studied. Samples were thermally cycled to 1,150 C and subjected to a water-drop test in order to elucidate the effect of water vapor on thermal barrier coating (TBC) spallation. It was shown that the addition of water promotes spallation of TBC samples after a given number of cycles at 1,150 C. This threshold was found to be equal to 170 cycles for the present system. Systems based on b-NiAl bond coat or on Pt-rich c/c0 bond coat were also sensitive to the water-drop test. Moreover, it was shown that water vapor in ambient air after minutes or hours at room temperature, promotes also TBC spallation once the critical number of cycles has been reached. This desktop spalling (DTS) can be prevented by locking up the cycled samples in a dry atmosphere box. These results for TBC systems confirm and document Smialek’s theory about DTS and moisture induced delayed spalling (MIDS) being the same phenomenon. Finally, the mechanisms implying hydrogen embrittlement or surface tension modifications are discussed

The Physical Connections Among IR QSOs, PG QSOs and Narrow-Line Seyfert 1 Galaxies

We study the properties of infrared-selected QSOs (IR QSOs), optically-selected QSOs (PG QSOs) and Narrow Line Seyfert 1 galaxies (NLS1s). We compare their properties from the infrared to the optical and examine various correlations among the black hole mass, accretion rate, star formation rate and optical and infrared luminosities. We find that the infrared excess in IR QSOs is mostly in the far infrared, and their infrared spectral indices suggest that the excess emission is from low temperature dust heated by starbursts rather than AGNs. The infrared excess is therefore a useful criterion to separate the relative contributions of starbursts and AGNs. We further find a tight correlation between the star formation rate and the accretion rate of central AGNs for IR QSOs. The ratio of the star formation rate and the accretion rate is about several hundred for IR QSOs, but decreases with the central black hole mass. This shows that the tight correlation between the stellar mass and the central black hole mass is preserved in massive starbursts during violent mergers. We suggest that the higher Eddington ratios of NLS1s and IR QSOs imply that they are in the early stage of evolution toward classical Seyfert 1's and QSOs, respectively.Comment: 32 pages, 6 figures, accepted by Ap

Multiwavelength Observations of one Galaxy in Marano Field

We report the multiwavelength observations of one intermediate redshift (z=0.3884) galaxy in the Marano Field. These data include ISOCAM middle infrared, VLT/FORS2 spectroscopic and photometric data, associated with the ATCA 1.4 GHz radio and ROSAT PSPC X-ray observations from literature. The Spectral Energy Distribution obtained by VLT spectroscopy exhibits its early-type galaxy property, while, in the same time, it has obvious [OIII]5007 emission line. The diagnostic diagram from the optical emission line ratios shows its Seyfert galaxy property. Its infrared-radio relation follows the correlation of sources detected at 15 \mu and radio. It has a high X-ray luminosity of 1.26*10^{43} ergs/s, which is much higher than the general elliptical galaxies s with the similar B band luminosity, and is about 2 orders of magnitude higher than the derived value from the star forming tracer, the FIR luminosity. This means that the X-ray sources of this galaxy are not stellar components, but the AGN is the dominant component.Comment: 6 pages, 1 PS figure and 4 tables. Publication in ChJAA, Suppl., the Special Issue for The Fifth Microquasar Workshop 2004: http://chjaa.bao.ac.cn/, 2005, Vol.5, 335-34

Sequential Recommendation Based on Multivariate Hawkes Process Embedding With Attention.

Recommender systems are important approaches for dealing with the information overload problem in the big data era, and various kinds of auxiliary information, including time and sequential information, can help improve the performance of retrieval and recommendation tasks. However, it is still a challenging problem how to fully exploit such information to achieve high-quality recommendation results and improve users' experience. In this work, we present a novel sequential recommendation model, called multivariate Hawkes process embedding with attention (MHPE-a), which combines a temporal point process with the attention mechanism to predict the items that the target user may interact with according to her/his historical records. Specifically, the proposed approach MHPE-a can model users' sequential patterns in their temporal interaction sequences accurately with a multivariate Hawkes process. Then, we perform an accurate sequential recommendation to satisfy target users' real-time requirements based on their preferences obtained with MHPE-a from their historical records. Especially, an attention mechanism is used to leverage users' long/short-term preferences adaptively to achieve an accurate sequential recommendation. Extensive experiments are conducted on two real-world datasets (lastfm and gowalla), and the results show that MHPE-a achieves better performance than state-of-the-art baselines

Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis

AKT3 gene is a constituent of the serine/threonine protein kinase family and plays a crucial role in synthesis of milk fats and cholesterol by regulating activity of the sterol regulatory element binding protein (SREBP). AKT3 is highly conserved in mammals and its expression levels during the lactation periods of cattle are markedly increased. AKT3 is highly expressed in the intestine followed by mammary gland and it is also expressed in immune cells. It is involved in the TLR pathways as effectively as proinflammatory cytokines. The aims of this study were to investigate the sequences differences between buffalo and cow. Our results showed that there were substantial differences between buffalo and cow in some exons and noteworthy differences of the gene size in different regions. We also identified the important consensus sequence motifs, variation in 2000 upstream of ATG, substantial difference in the “3′UTR” region, and miRNA association in the buffalo sequences compared with the cow. In addition, genetic analyses, such as gene structure, phylogenetic tree, position of different motifs, and functional domains, were performed to establish their correlation with other species. This may indicate that a buffalo breed has potential resistance to disease, environment changes, and airborne microorganisms and some good production and reproductive traits

The damping properties of the foam‑filled shaft of primary feathers of the pigeon Columba livia

The avian feather combines mechanical properties of robustness and flexibility while maintaining a low weight. Under periodic and random dynamic loading, the feathers sustain bending forces and vibrations during flight. Excessive vibrations can increase noise, energy consumption, and negatively impact flight stability. However, damping can alter the system response, and result in increased stability and reduced noise. Although the structure of feathers has already been studied, little is known about their damping properties. In particular, the link between the structure of shafts and their damping is unknown. This study aims at understanding the structure-damping relationship of the shafts. For this purpose, laser Doppler vibrometry (LDV) was used to measure the damping properties of the feather shaft in three segments selected from the base, middle, and tip. A combination of scanning electron microscopy (SEM) and micro-computed tomography (μCT) was used to investigate the gradient microstructure of the shaft. The results showed the presence of two fundamental vibration modes, when mechanically excited in the horizontal and vertical directions. It was also found that the base and middle parts of the shaft have higher damping ratios than the tip, which could be attributed to their larger foam cells, higher foam/cortex ratio, and higher percentage of foam. This study provides the first indication of graded damping properties in feathers