The Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus

The cell wall teichuronic acid (TUA) of Micrococcus luteus is a long-chain polysaccharide composed of disaccharide repeating units [-4-β-D-ManNAcAp-(1→6)α-D-Glcp−1-]n, which is covalently anchored to the peptidoglycan on the inner cell wall and extended to the outer surface of the cell envelope. An enzyme complex responsible for the TUA chain biosynthesis was purified and characterized. The 440kDa enzyme complex, named teichuronic acid synthetase (TUAS), is an octomer composed of two kinds of glycosyltransferases, Glucosyltransferase, and ManNAcA-transferase, which is capable of catalyzing the transfer of disaccharide glycosyl residues containing both glucose and the N-acetylmannosaminuronic acid residues. TUAS displays hydrophobic properties and is found primarily associated with the cytoplasmic membrane. The purified TUAS contains carotinoids and lipids. TUAS activity is diminished by phospholipase digestion. We propose that TUAS serves as a multitasking polysaccharide assembling station on the bacterial membrane.National Institute of Allergy and Infectious Diseases (Public Health Service Grants AI-08295); American Lung Association (RG-107-N

Numerical investigations of low-density nozzle flow by solving the Boltzmann equation

A two-dimensional finite-difference code to solve the BGK-Boltzmann equation has been developed. The solution procedure consists of three steps: (1) transforming the BGK-Boltzmann equation into two simultaneous partial differential equations by taking moments of the distribution function with respect to the molecular velocity u(sub z), with weighting factors 1 and u(sub z)(sup 2); (2) solving the transformed equations in the physical space based on the time-marching technique and the four-stage Runge-Kutta time integration, for a given discrete-ordinate. The Roe's second-order upwind difference scheme is used to discretize the convective terms and the collision terms are treated as source terms; and (3) using the newly calculated distribution functions at each point in the physical space to calculate the macroscopic flow parameters by the modified Gaussian quadrature formula. Repeating steps 2 and 3, the time-marching procedure stops when the convergent criteria is reached. A low-density nozzle flow field has been calculated by this newly developed code. The BGK Boltzmann solution and experimental data show excellent agreement. It demonstrated that numerical solutions of the BGK-Boltzmann equation are ready to be experimentally validated

The Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus

The cell wall teichuronic acid (TUA) of Micrococcus luteus is a long-chain polysaccharide composed of disaccharide repeating units [-4-β-D-ManNAcAp-(1→6)α-D-Glcp−1-]n, which is covalently anchored to the peptidoglycan on the inner cell wall and extended to the outer surface of the cell envelope. An enzyme complex responsible for the TUA chain biosynthesis was purified and characterized. The 440 kDa enzyme complex, named teichuronic acid synthetase (TUAS), is an octomer composed of two kinds of glycosyltransferases, Glucosyltransferase, and ManNAcA-transferase, which is capable of catalyzing the transfer of disaccharide glycosyl residues containing both glucose and the N-acetylmannosaminuronic acid residues. TUAS displays hydrophobic properties and is found primarily associated with the cytoplasmic membrane. The purified TUAS contains carotinoids and lipids. TUAS activity is diminished by phospholipase digestion. We propose that TUAS serves as a multitasking polysaccharide assembling station on the bacterial membrane

The Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus

The cell wall teichuronic acid (TUA) of Micrococcus luteus is a long-chain polysaccharide composed of disaccharide repeating units [-4-β-D-ManNAcAp-(1→6)α-D-Glcp−1-]n, which is covalently anchored to the peptidoglycan on the inner cell wall and extended to the outer surface of the cell envelope. An enzyme complex responsible for the TUA chain biosynthesis was purified and characterized. The 440 kDa enzyme complex, named teichuronic acid synthetase (TUAS), is an octomer composed of two kinds of glycosyltransferases, Glucosyltransferase, and ManNAcA-transferase, which is capable of catalyzing the transfer of disaccharide glycosyl residues containing both glucose and the N-acetylmannosaminuronic acid residues. TUAS displays hydrophobic properties and is found primarily associated with the cytoplasmic membrane. The purified TUAS contains carotinoids and lipids. TUAS activity is diminished by phospholipase digestion. We propose that TUAS serves as a multitasking polysaccharide assembling station on the bacterial membrane

Modeling of Non-Spherical Droplet Dynamics

A two-dimensional time-dependent computer code based on the modified Arbitrary Lagrangian Eulerian (ALE) technique, has been developed to simulate non-spherical droplet dynamics and evaporation under convective flows at real rocket combustion chamber conditions. The equations of mass, momentum, energy and species are simultaneously solved for both liquid and gas phases with an accurate dynamic interface tracking. The jump boundary conditions across the deforming droplet surface are obtained by applying the integral forms of conservation of mass, momentum, and energy. At each time step, the interface geometry and flow properties at the droplet surface are implicitly solved by satisfying the interface boundary conditions. A Lagrangian technique was developed to track the arbitrarily moving interface between the liquid droplet and the external gas. An elliptic grid generator is adopted to dynamically reconstruct grids both inside and outside the droplet surface. This code has been used to study droplet oscillation, droplet deformation/breakup, nonspherical droplet evaporation in both low and high pressure convective flows. This presentation briefly describes the numerical algorithm for modeling of the nonspherical droplet dynamics and demonstrates the representative simulation results of nonspherical droplet evaporation at low and high pressure convective flows. Potential applications of this code to rocket combustor design and performance predictions are discussed

Study of DX center in Cd0.8Zn0.2Te:CI by positron annihilation

Variable energy positron beam and positron annihilation lifetime experiments have been carried out to study the DX center in Cd0.8Zn0.2Te:Cl at 50 K. A short positron effective diffusion length of 275±25 Å and a large intensity of 79.0%±0.3% for the long lifetime component indicate a strong trapping effect at DX centers. A trapping rate of κ=1.53±0.05×109 s-1 and a positron lifetime of 335±2 ps at the DX center were obtained. The concentration of DX centers is found to be 5.9 ±0.7×1016 cm-3, which is in good agreement with the results obtained using Hall effect and thermo-electric effect measurements. © 1998 American Institute of Physics.published_or_final_versio

Exhausted Plume Flow Field Prediction Near the Afterbody of Hypersonic Flight Vehicles in High Altitudes

A two-dimensional computer code to solve the Burnett equations has been developed which computes the flow interaction between an exhausted plume and hypersonic external flow near the afterbody of a flight vehicle. This Burnett-2D code extends the capability of Navier-Stokes solver (RPLUS2D code) to include high-order Burnett source terms and slip-wall conditions for velocity and temperature. Higher-order Burnett viscous stress and heat flux terms are discretized using central-differencing and treated as source terms. Blocking logic is adopted in order to overcome the difficulty of grid generation. The computation of exhaust plume flow field is divided into two steps. In the first step, the thruster nozzle exit conditions are computed which generates inflow conditions in the base area near the afterbody. Results demonstrated that at high altitudes, the computations of nozzle exit conditions must include the effects of base flow since significant expansion exists in the base region. In the second step, Burnett equations were solved for exhaust plume flow field near the afterbody. The free stream conditions are set at an altitude equal to 80km and the Mach number is equal to 5.0. The preliminary results show that the plume expansion, as altitude increases, will eventually cause upstream flow separation