Regeneration and Healing of Bone and Cartilage in Type-1 and Type-2 Diabetes: The Effects of Insulin

Increased fragility of long bones and delay in fracture healing in adults as well as skeletal development disorders in children are thought to be associated with endochondral ossification problems. The extracellular matrix (ECM) of cartilage is absorbed during endochondral ossification. During the recovery process from damage, chondrocytes undergo hypertrophy and increase their cell volume; on the other hand, it is also necessary to digest the ECM elements around the cells. Recent research on the proteolytic enzymes in ECM assumed to be responsible for the digestion is connected to proteinases digesting type II collagen and aggrecan. ADAMTS1, 4, and 5 are known as major aggrecanases within the matrix metalloproteinase (MMP) enzymes in human cartilage. These are regarded as main determiners among MMPs of bone growth, healing, and remodeling. In this mini review article, the remodeling and restoration of osseous tissue in type-1 and -2 diabetes mellitus (DM) and the effect of insulin on these processes are discussed briefly in the light of the current literature.WoSScopu

Hla-G ve kanser İlişkisi

The importance of HLA antigens on immunity and tissue compatibility is known. The suppressor role of a non classical HLA antigen HLA-G is being clearer with the results of last studies. On normal situations its being is restricted in a few type of tissue. It can be found on fetal tissues like trophoblasts, tymic medulla, cornea, pancreatic islets and precursors of erithroid and endothelial cells. The expression of HLA-g is increased in the situations like malignities, transplantation, multiple sclerosis, inflammatory diseases and viral infections. In this review it is aimed to give short information on HLA system and to introduce the studies pronouncing the relation of HLA-G and malignities. © 2012 Düzce Medical Journal

Relationship of maternal serum resistin and visfatin levels with gestational diabetes mellitus

WOS: 000334743400007PubMed: 24512558Introduction: Adiponectin, resistin and visfatin are thought to play role in the pathophysiology of gestational diabetes (GDM). In this study, we aimed to investigate the association of maternal second trimester serum resistin and visfatin levels with GDM. Materials and methods: Screening and diagnosis for GDM was performed between the 24-28th gestational weeks. About 40 women diagnosed with GDM and 40 non-diabetic women constituted the study and control groups, respectively. Groups were compared for second trimester maternal serum resistin, visfatin and HbA1c levels, HOMA-IR and postpartum 75 g OGTT results. Results: Mean serum resistin (p = 0.071) and visfatin (p = 0.194) levels were similar between the groups. However, mean BMI (p = 0.013), HOMA-IR (p = 0.019), HbA1c (p<0.0001) and birth weight (p = 0.037) were significantly higher in GDM group compared to controls. Type 2 diabetes and impaired glucose tolerance were detected in 2 (5%) and 7 (20%) women in the GDM group, respectively, with 75 g OGTT performed at the postpartum 6th week. Resistin levels of patients with GDM and postpartum glucose intolerance were higher than those with GDM but no postpartum glucose intolerance (p = 0.012). Visfatin levels in the GDM group showed a positive correlation with biparietal diameter, head circumference, abdominal circumference and femur length (p<0.05). Conclusion: Maternal serum resistin and visfatin levels are unchanged in GDM. In patients with GDM, second trimester resistin levels may be predictive for postpartum glucose intolerance and second trimester visfatin levels may be related with fetal biometric measurements. Further larger studies are needed.Duzce University, Medical Faculty Scientific Study Research CommissionDuzce University [2012.04.HD.060]The authors report no declarations of interest. This study was financially endorsed by Duzce University, Medical Faculty Scientific Study Research Commission (registration number 2012.04.HD.060)

The Relation Between Hla-G And Cancer

HLA antijenlerinin doku uyumu ve immünite açısından önemi bilinmektedir. HLA sınıf 1 antijenlerinden non-klasik gruba dâhil olan HLA-Gnin kanser immunitesindeki baskılayıcı rolü son zamanlarda yapılan çalışmalarda daha da açığa çıkarılmıştır. HLA-G normal durumlarda çok kısıtlı dokularda bulunmaktadır. Trofoblastlar gibi fetal dokularda, timik medulla, kornea, pankreatik adacıklarda ve eritroid ve endotelial hücre prekürsörlerinde bulunmaktadır. HLA-G ekspresyonu bu fizyolojik durumların dışında kanserler, transplantasyon, multiple scleroz, inflamatuar hastalıklar ve viral enfeksiyonlar gibi patolojik durumlarda da artmış seviyelerdedir. Bu derlemede HLA sistemi hakkında kısaca bilgi verilerek HLA-G ile kanser ilişkisini vurgulayan farklı kanser türleri üzerinde yapılmış çalışmaları ortaya koymak amaçlanmıştır.The importance of HLA antigens on immunity and tissue compatibility is known. The suppressor role of a non classical HLA antigen HLA-G is being clearer with the results of last studies. On normal situations its being is restricted in a few type of tissue. It can be found on fetal tissues like trophoblasts, tymic medulla, cornea, pancreatic islets and precursors of erithroid and endothelial cells. The expression of HLA-g is increased in the situations like malignities, transplantation, multiple sclerosis, inflammatory diseases and viral infections. In this review it is aimed to give short information on HLA system and to introduce the studies pronouncing the relation of HLA-G and malignities

Phototherapy, Hyperbilirubinemia and Genotoxicity in Newborns

Tatli, Mansur/0000-0003-2767-3734WOS: 000276873000011PubMed: 20096372

Ankilozan spondilit hastalarında nötrofil- lenfosit oranı

Amaç: Bu çalışmanın amacı, ankilozan spondilit (AS) hastalarındaki inflamasyon ile nötrofil -lenfosit oranı (NLO) arasındaki ilişkiyi belirlemektir. Yöntem: Ankilozan spondilit tanılı 40 hasta ve 30 sağlıklı gönüllü çalışmaya dahil edildi. Tüm veriler yatan ve poliklinik hasta kayıt veritabanından elde edildi. Tüm olguların Bath Ankilozan Spondilit Hastalık Aktivite İndeksi (BA S- DAI), tam kan sayımı (TKS), CRP ve ESHı da kapsıyan ayrı ntılı fizik muayene ve laboratuvar bulguları kaydedildi. Lenfosit ve nötrofil sayısı otomatik TKSnin bir parçası olarak ölçüldü. Bulgular : Akut-faz reaktanı olan CRP ve ESH kontrol gr ubuna göre hasta grubunda anlamlı olarak daha yüksekti (p 0.05). Sonuç : Bu çalışmada NLO düzeylerinin AS hastalarında sağlıklı bireylere göre anlamlı olarak yüksek olmadığı görüldü. Bu sonuç NLOnun AS hastalarında sistemik in flamasyonu değerlendirmede uygun bir ölçü birimi olmad ığını götermektedir. Bizim elde ettiğimiz bu ön sonuçların daha ileri çalışmalarla tamamlanması gerektiğine inanıyoruz.Objective : The purpose of the present study is to determine the association between neutrophil -lymphocyte ratio (NLR) and inflammation in ankylosing spondylitis (AS). Method : Forty patients with ankylosing spondylitis and 30 healthy volunteers were included in the study. All data were obtained from patient registry database from in -patient and out-patient clinics. Detailed physical examination, and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) were record ed. Complete blood count (CBC), CRP and ESR were performed recorded as laboratory tests in all participants. Lymphocyte and neutrophil counts were measured as part of the automated CBC. Results : The CRP, which is an acute -phase reactant, and ESR were signi ficantly higher in the patient group than those in the control group (p0.05). Conclusion : We observed that NLR levels werent significantly higher in AS patients compared to healthy individuals. NLR hasnt seem a reasonable measure to detect systemic infla m- mation in AS patients. We believe that our preliminary results need to be complemented with further studies

Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C

We have investigated the effect of subchronic administration of methidathion (MD) on ovary evaluated ameliorating effects of vitamins E and C against MD toxicity. Experimental groups were as follows: control group; a group treated with 5 mg/kg body weight MD (MD group); and a group treated with 5 mg/kg body weight MD plus vitamin E and vitamin C (MD + Vit group). MD and MD + Vit groups were given MD by gavage five days a week for four weeks at a dose level of 5 mg/kg/day by using corn oil as the vehicle. Serum malondialdehyde (MDA: an indicator of lipid peroxidation) concentration, serum activity of cholinesterase (ChE), and ovary histopathology were studied. The level of MDA increased significantly in the MD group compared with the control (P < 0.005). Serum MDA decreased significantly in the MD + Vit group compared with the MD group (P < 0.05). The activities of ChE decreased significantly both in the MD and MD + Vit groups compared with the controls (P < 0.05). However, the decrease in the MD + Vit groups was less than in the MD group; the ChE activity in the MD + Vit group was significantly higher compared with MD group (P < 0.05). Number of ovarian follicles were significantly lower in the MD group compared to the controls (P < 0.05). Number of atretic follicles were significantly higher in the MD group than in the controls (P < 0.05). Follicle counts in MD + Vit group showed that all types of ovarian follicles were significantly higher, and a significant decrease in the number of atretic follicles compared with the MD group (P < 0.05). In conclusion, subchronic MD administration caused an ovarian damage, in addition, LPO may be one of the molecular mechanisms involved in MD-induced toxicity. Treatment with vitamins E and C after the administration of MD reduced LPO and ovarian damage

Serum visfatin and omentin levels in slow coronary flow

WOS: 000347270500006PubMed: 25481776Objective: The adipocytokines visfatin and omentin have a direct effect on inflammation and endothelial injury. The expression of visfatin is closely associated with the expression of proin-flam matory cytokines. Omentin has an anti-inflammatory effect and is inversely associated with coronary artery disease (CAD). The slow coronary flow phenomenon is an angiographic finding characterized by delayed distal vessel opacification in the absence of significant epicardial coronary disease. The pathophysiology of SCF has not been clearly identified, although multiple abnormalities including endothelial dysfunction, atherothrombosis and inflammation have been reported. However, the relationship between visfatin, omentin and SCF is still unknown. In this study, we aimed to investigate the relationship of these adipocytokines with SCF. Methods: The study included slow coronary flow (n=45) and normal coronary flow (n=55) subjects, according to the corrected TIMI frame count, who underwent angiography in the catheterization laboratory of Duzce University. Statistical analyses were performed with SPSS version 12. Results: Visfatin levels were significantly higher in patients with SCF than in controls (p<0.001). Plasma omentin levels were lower in the SCF group than in controls, although without statistical significance. Visfatin, gender and platelet count were significant predictors of SCF in multivariate logistic regression analysis (OR 0.748, 95% CI 0.632-0.886, p=0.01; OR 30.016, 95% CI 4.355-206.8, p=0.01; OR1.028, 95% CI 1.006-1.050, p=0.011, respectively). Conclusion: Adipocytokines such as visfatin and omentin may play a role in the pathogenesis of coronary slow flow. (C) 2014 Sociedade Portuguesa de Cardiologia. Published by Elsevier Espana, S.L.U. All rights reserved

High sensitive CRP and sICAM-1 can predict Major Adverse Cardiovascular Events: MELEN Study: A Large Turkish Population Based Study

WOS: 000372103700004Objective: We aimed to evaluate the predictors of major adverse cardiovascular events (MACE) in a prospective population based study. Methods: This study included 153 participants aged>40 years with high and very high cardiovascular risk, and 50 participants aged>40 years with low cardiovascular risk according to the SCORE risk assessment. All the participants underwent a doppler ultrasound examination of carotid intima media thickness (CIMT), echocardiographic examination, ECG recording and various biochemical analyses. High-sensitivity C-reactive protein (hsCRP) was measured with chemiluminescent immunometric assay, serum amyloid-A (SAA) protein, soluble intercellular adhesion molecule-1 (sICAM), apolipoprotein-B (ApoB) were measured with ELISA method. eNOS single-nucleotide polymorphism was detected using polymerase chain reaction and restriction fragment length polymorphism methods. The follow-up was done 36 months after the baseline admission. MACE was defined as cardiovascular mortality or myocardial infarction or stroke. Results: Frequency of MACE was higher in high and very high risk group according to low risk group. There were no significant differences in eNOS gene polymorphisms between the risk groups and control subjects, and no significant association between eNOS gene polymorphisms and MACE was detected. Age, ejection fraction (EF), CIMT, hsCRP, ApoB, sICAM-1, and SAA protein levels were all significantly associated with MACE in univariate logistic regression analyses. Multivariate analyses revealed that age (OR: 1.08, CI: 1.02-1.15, p=0.013), EF (OR: 0.94, CI: 0.89-0.99, p=0.016), hsCRP (OR: 1.36, CI: 1.12-1.67, p=0.003) and sICAM-1 (OR: 81.0, CI: 1.04-6320, p=0.048) levels were the only independent predictors of MACE. Conclusion: Higher age, hsCRP and sICAM-1 levels and lower EF were independent predictors of MACE

Oxidative Stress and Decrease of Paroxonase Activity in Patients whit Prostate Cancer

WOS: 000363103100008Objective: Prostate cancer is the leading cause of cancer-related deaths. Oxidative DNA damage may contribute to the prostate cancer. The paraoxonase (PON1) is an endogenous antioxidant in the human body. The aim of our study was to determine whether lipid parameters, total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSG), serum paraoxonase (PON1) and arylesterase (ARE) levels and phenotypes distribution alter new diagnosis in patients with prostate cancer and to compare the values with those of healthy controls. Methods: The study was performed prospective which consist of the prostate cancer group (PC) and healthy control group. Serum PON1, ARE activities, and other parameters were measured in 40 subjects in both groups. The PON1 phenotypes were defined according to the ratio of serum PON1/ARE activity. In statistical evaluation of data was performed by Student t test and Pearson's correlation analysis. Results: TKOL and LDL-K levels were found to be lower in the patients compared to controls (p=0,044; p=0,026). OSI levels in patients was higher than the controls (p=0,029). PON1 and ARE activities were found to be lower in patients compared to the controls (p=0,040; p=0,027). PON1 enzyme activity was determined as three different phenotypes in both groups. In PC group, significant deviation of PON1 phenotype frequencies from Hardy-Weinberg equilibrium was found. Conclusion: The results of our study suggest that oxidative stress, through lipid peroxidation may play an important role for the development of prostate cancer and that PON1, and PON1 phenotyping may be predictive for prostate cancer