Mapping of QTLs for leaf area and the association with winter hardiness in fall-sown lentil

Variations in plant architecture are often associated with the ability of plants to survive cold stress during winter. In studies of winter hardiness in lentil, it appeared that small leaf area was associated with improved winter survival. Based on this observation, the inheritance of leaf area and the relationship with winter hardiness using an F6 - derived recombinant inbred line (RIL) population from the cross of WA8649090 x Precoz was investigated. The WA8649090 parent was winter hardy and had small leaves, while the Precoz parent was non-winter hardy and had large leaves. The 106 RILs and the parents were planted in a field in a randomized complete block design with three replications. Leaf area(cm2) of leaves from the fourth node was measured using a flatbed scanner and WinRHIZO software. Average leaf area for WA8649090 was 0.46 cm2, while leaf area for Precoz was 0.89 cm2. Average leaf area of the RILs was 0.63 cm2, and the frequency distribution was continuous, indicating the effects of more than one gene. Quantitative trait locus (QTL) analysis using a 130-point linkage map revealed one major QTL on linkage group 6 which explained 20.45% of the phenotypic variation for leaf area. The location of QTL for leaf area mapped the same region where one of the QTL for winter hardiness was mapped and significant association (r2 = 0.750, P< 0.01) was found between leaf area and winter hardiness. These results indicated an association between winter hardiness and leaf area that provides information applicable to lentil breeding.Keywords: Leaf area, lentil, recombinant inbred line (RIL), QTL mapping, winter hardines

Effect of different defoliants and application times on the yield and quality components of cotton in semi-arid conditions

This study was conducted to determine the effect of different defoliants (Dropp ultra® (DU): thidiazuron+diuron and Roundup (RU): glyphosate) and application times [60, 75 and 90 days after flowering (DAF)] on cotton. The research was carried out at the Harran University, Faculty of Agriculture Research and Application Center in 2001 and 2002 using cotton variety cv. Stoneville-453. Experiments were arranged as split plot design with three replications. Defoliations were at the main plots and application times at the subplots. Experimental plots were consisted of six rows, 10 m in length, interrow was 0.70 m and intra-row spacing was 0.20 m. The results of the study indicated that the application of DU defoliant at 60 days after flowering reduced seed cotton yield, number of bolls, boll weight and lint index. With this, seed cotton yield, number of bolls, boll weight and lint index increased with delayed defoliation time in both years. Also, there were no statistically significant differences between the treatments in terms of ginning outturn, fiber length, fiber strength and fiber fineness. It was also found that the application of 2000 cc ha-1 RU was not enough as a dose to affect leaf defoliation and other investigated components

Diurnal gradual heat stress affects antioxidant enzymes, proline accumulation and some physiological components in cotton (Gossypium hirsutum L.)

Even though high temperatures significantly reduce both vegetative growth and yield in cotton, very little is known about the effects of heat stress on cotton antioxidant system. Thus, the effects of gradual heat stress on cotton growth in controlled conditions were investigated in the present study. At squaring stage, cotton plants were subjected to two different temperatures, 38 and 45°C to determine the influence of heat stress on the plants. The results of the present study showed that heat stress did not significantly altered the levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in the leaves, whereas there was a remarkable decline in proline quantity of the leaves of plants subjected to 45°C heat stress. As for the amount of total chlorophyll content, a slight increase at plants treated with 38°C temperature was observed. Furthermore, the activities of some enzymes such as superoxide dismutase (SOD), which were associated with heat stress response in other plants was also investigated. For example, there was decline in the activitity of SOD in the plants exposed to high temperatures. On the contrary, catalase (CAT) activity increased at 45°C; peroxidase (POX) activity increased at 38°C and ascorbate peroxidase (APX) activity increased at 38 and 45°C. The results from this study suggest a potential role for CAT, POX and APX in the reduction of elevated levels of H2O2 in cotton plants grown under heat stress condition. To sum up, it could be concluded that, diurnal gradual heat stress caused a low oxidative injury in cotton

Estimation of some stand parameters using digital aerial photographs for conservation and service oriented forests

Forest inventory, which is the first step of forest management planning, is the most difficult stage that requires much time and a lot of efforts. To reduce fieldworks that are considered time consuming and expensive methods of ground measurements, remote sensing data are widely used. Aerial photographs have been an integral part of forest inventory data in Turkey since 1963. Panchromatic and RGBI (Red, Green, Blue, Infrared) aerial photographs acquired by digital aerial cameras proved to be very important in forest inventory. They have maintained their importance for forest management planning process. The aim of this study is to construct a fast and practical inventory model that requires least fieldwork for forest management planning process. Pixel values and vegetation indices (NDVI, DVI, IPVI, RVI and PCA), obtained from remote sensing data, and stand parameters (stand volume, volume increment and number of trees) have been compared statistically. Black pine Pinus nigra J. F. Arnold plantations located in the south-east region of Turkey, Çelikhan Forest Planning Unit, was chosen as a research area. 0.5 meter spacing and 8 bit radiometric resolution Ultracam-X Digital Aerial Photos were used as remote sensing data. According to statistical analysis, IPVI and Green Band values provided the highest evaluation coefficient compared to the models developed for the estimation of stand parameters. Adjusted R square of stand volume, volume increment and the number of tree in the models were found to yield 0.74, 0.73 and 0.50 respectively. It was concluded that stand characteristics estimated by statistical models can be used for forest areas managed for conservation and service purposes

Pregnancy and Crimean-Congo haemorrhagic fever

AbstractCrimean-Congo Hemorrhagic fever (CCHF) is a potentially fatal viral infection with reported case fatality rates of 5–30%. Humans become infected through tick bites, by contact with a patient with CCHF during the acute phase of infection, or by contact with blood or tissues from viraemic livestock. In this first report in the literature, we present the characteristics of three pregnant women with CCHF infection and the outcome of their babies. Transmission of the CCHF infection could be either intrauterine or perinatal. In endemic regions, CCHF infection should be considered in the differential diagnosis of HELLP syndrome (haemolytic anaemia, elevated liver enzymes, low platelet count), and obstetricians should be familiar with the characteristics of CCHF infection. In the aetiology of necrotising enterocolitis, CCHF should be considered

Spatial Analysis of Urban Form and Pedestrian Exposure to Traffic Noise

In the Macao Peninsula, the high population density (49,763 inhabitants/km2) and the lack of control over the number of vehicles (460 vehicles/km) have led to an increase in urban pollution. To provide useful information to local government and urban planners, this paper investigates the spatial distribution of traffic noise in the Macao Peninsula. The interactions among urban form, traffic flow and traffic noise are addressed. Considering the spatial nature of urban geometry and traffic, a high-resolution GIS-based traffic noise model system is applied. Results indicate that the Macao Peninsula has fallen into a situation of serious traffic noise pollution. About 60% of traffic noise levels along the major pedestrian sidewalks in the evening peak hour exceed the National Standard of 70 dB(A) in China. In particular, about 21% of traffic noise levels along the pedestrian sidewalks are above the National Standard by 5 dB(A). Noticeably, the high pedestrian exposure to traffic noise in the historical urban area reduces the comfort of tourists walking in the historic centre and is ruining the reputation of the area as a World Cultural Heritage site

Boundary Lubrication: Squeeze-out Dynamics of a Compressible 2D Liquid

The expulsion dynamics of the last liquid monolayer of molecules confined between two surfaces has been analyzed by solving the two-dimensional (2D) Navier-Stokes equation for a compressible liquid. We find that the squeeze-out is characterized by the parameter g0 ~ P0/(rho c^2), where P0 is the average perpendicular (squeezing) pressure, rho the liquid (3D) density and c the longitudinal sound velocity in the monolayer film. When g0 << 1 the result of the earlier incompressible treatment is recovered. Numerical results for the squeeze-out time, and for the time-dependence of the radius of the squeezed-out region, indicate that compressibility effects may be non-negligible both in time and in space. In space, they dominate at the edge of the squeeze-out region. In time, they are strongest right at the onset of the squeeze-out process, and just before its completion.Comment: revtex4, 6 pages, 4 figures. Published on PRB on December 31, 200

Thermodynamic analysis of regulation in metabolic networks using constraint-based modeling

<p>Abstract</p> <p>Background</p> <p><it>Geobacter sulfurreducens </it>is a member of the <it>Geobacter </it>species, which are capable of oxidation of organic waste coupled to the reduction of heavy metals and electrode with applications in bioremediation and bioenergy generation. While the metabolism of this organism has been studied through the development of a stoichiometry based genome-scale metabolic model, the associated regulatory network has not yet been well studied. In this manuscript, we report on the implementation of a thermodynamics based metabolic flux model for <it>Geobacter sulfurreducens</it>. We use this updated model to identify reactions that are subject to regulatory control in the metabolic network of <it>G. sulfurreducens </it>using thermodynamic variability analysis.</p> <p>Findings</p> <p>As a first step, we have validated the regulatory sites and bottleneck reactions predicted by the thermodynamic flux analysis in <it>E. coli </it>by evaluating the expression ranges of the corresponding genes. We then identified ten reactions in the metabolic network of <it>G. sulfurreducens </it>that are predicted to be candidates for regulation. We then compared the free energy ranges for these reactions with the corresponding gene expression fold changes under conditions of different environmental and genetic perturbations and show that the model predictions of regulation are consistent with data. In addition, we also identify reactions that operate close to equilibrium and show that the experimentally determined exchange coefficient (a measure of reversibility) is significant for these reactions.</p> <p>Conclusions</p> <p>Application of the thermodynamic constraints resulted in identification of potential bottleneck reactions not only from the central metabolism but also from the nucleotide and amino acid subsystems, thereby showing the highly coupled nature of the thermodynamic constraints. In addition, thermodynamic variability analysis serves as a valuable tool in estimating the ranges of Δ_rG' of every reaction in the model leading to the prediction of regulatory sites in the metabolic network, thereby characterizing the regulatory network in both a model organism such as <it>E. coli </it>as well as a non model organism such as <it>G. sulfurreducens</it>.</p