A fountain of droplets

A vessel is plunged upside down into a pool of 50 cSt silicone oil. An air bell is then created. This bell is vertically shaken at 60 Hz that leads to the oscillation of the air/oil interface. The edges of the immersed vessel generate surface waves that propagate towards the center of the bell. When the amplitude of the oscillation increases, wave amplitude increases. We study the influence of the angle between successive sides on the wave patterns. Two kinds of vessel have been studied: a triangular and a square prism. The shape of the air/oil meniscus depends on the angle between the sides of the considered prism. As the amplitude of the oscillation is increased, the triple line, which is the contact line between the solid and the air/oil interface, moves up and down. Above a given acceleration that depends on the immersion depth and on the shape vessel, wave goes under the corner edge of the bell. During the oscillation, the wave generates at the edges presents a singularity that leads eventually to a jet and a drop ejection. A drop is ejected at each oscillation. More complicated ejection can be produced with further increase of the amplitude. This is a sample arXiv article illustrating the use of fluid dynamics videos.Comment: 3 pages, 2 figures, 2 movies (high-res and low-res

Utilisation de la couleur pour l'appariement et l'indexation d'images.

De nombreux travaux récents ont montré que l'utilisation directe du signal des images en niveaux de gris permet de développer des systèmes puissants d'appariement, d'indexation et de reconnaissance d'images. Le présent document explore l'utilisation d'images en couleur pour ces mêmes objectifs. Les principaux points en sont le choix du modèle de variation de la couleur, l'utilisation d'invariants colorimétriques, et la normalisation des images

Capillary Bridge Formation and Breakage: A Test to Characterize Antiadhesive Surfaces

In order to characterize very weak adhesive surfaces, we have developed a quantitative test inspired by the Johnson, Kendall, and Roberts adhesion test for soft adhesives, which relies on the formation and then the rupture of a capillary bridge between the surface to be tested and a liquid bath. Both the shape and the kinetics of breakage of the capillary bridge for various coatings put into contact with liquids of various viscosities and surface tensions have been studied. Several pull off regimes can be distinguished. For low pull off velocities, a quasi-static regime is observed, well described by capillary equations and sensitive to the hysteresis of the contact angle of the fluid on the coating. Above a critical pull off velocity that depends on the fluid viscosity, a dynamic regime is observed, characterized by the formation of a flat pancake of fluid on the coating that recedes more slowly than the capillary bridge itself. After the breakage of the capillary bridge, a small drop can remain attached to the surface. The volume of this drop depends on the dynamical regime and is strongly affected by very small differences between the coatings. The aptitude of this test in characterizing very weakly adhesive surfaces is exemplified by a comparison between three different perfluorinated coatings

Analyzing symmetry breaking within a chaotic quantum system via Bayesian inference

Bayesian inference is applied to the level fluctuations of two coupled microwave billiards in order to extract the coupling strength. The coupled resonators provide a model of a chaotic quantum system containing two coupled symmetry classes of levels. The number variance is used to quantify the level fluctuations as a function of the coupling and to construct the conditional probability distribution of the data. The prior distribution of the coupling parameter is obtained from an invariance argument on the entropy of the posterior distribution.Comment: Example from chaotic dynamics. 8 pages, 7 figures. Submitted to PR

Fam65b is a new transcriptional target of FOXO1 that regulates RhoA signaling for T lymphocyte migration

Forkhead box Os (FOXOs) transcription factors favor both T cell quiescence and trafficking through their control of the expression of genes involved in cell cycle progression, adhesion and homing. Here, we report that the product of the fam65b gene is a new transcriptional target of FOXO1 that regulates RhoA activity. We show that Fam65b binds the small GTPase RhoA via a non canonical domain and represses its activity by decreasing its GTP loading. As a consequence, Fam65b negatively regulates chemokine-induced responses such as adhesion, morphological polarisation and migration. Therefore, these results show the existence of a new functional link between FOXO1 and RhoA pathways, through which the FOXO1 target Fam65b tonically dampens chemokine-induced migration by repressing RhoA activity

Atypical strain of Toxoplasma gondii causing fatal reactivation after hematopoietic stem cell transplantion in a patient with an underlying immunological deficiency

International audienceIn immunocompromized patients, including hematopoietic stem cell transplant (HSCT) recipients, life-threatening toxoplasmosis may result from reactivation of previous infection. We report a case of severe disseminated toxoplasmosis that developed early after allogeneic HSCT for T-cell lymphoblastic leukemia/lymphoma in a 15-year-old Toxoplasma gondii-seropositive boy with Nijmegen breakage syndrome, a rare genetic DNA repair disorder associated with immunodeficiency. The donor was the patient's HLA-identical brother. Prophylaxis with cotrimoxazole was discontinued a day before the HSCT procedure. Signs of lung infection appeared as early as day 14 post-HSCT. The presence of tachyzoite-like structures on Giemsa-stained bronchoalveolar lavage (BAL) fluid smears suggested toxoplasmosis. Real-time PCR targeted at the T. gondii AF146527 gene revealed extremely high parasite burdens in both blood and BAL fluid. Although immediate introduction of specific treatment resulted in a marked reduction of the parasite load and transient clinical improvement, the patient deteriorated and died of multiple organ failure on day 39 post-HSCT. Direct genotyping of T. gondii DNA from blood and BAL fluid with the PCR-restriction fragment length polymorphism method revealed type II alleles with SAG1, SAG2, and GRA6 markers but alleles of both type I and type II with GRA7. Additional analysis with 15 microsatellite markers showed that the T. gondii DNA was atypical and genetically divergent from that of the clonal type I, II, and III strains. This is the first report of increased clinical severity of toxoplasmosis associated with an atypical strain in the setting of immunosuppression, which emphasizes the need to diagnose and monitor toxoplasmosis by quantitative molecular methods in cases of reactivation risk

Intercellular Transfer of Oncogenic H-Ras at the Immunological Synapse

Immune cells establish dynamic adhesive cell–cell interactions at a specific contact region, termed the immunological synapse (IS). Intriguing features of the IS are the formation of regions of plasma membrane fusion and the intercellular exchange of membrane fragments between the conjugated cells. It is not known whether upon IS formation, intact intracellular proteins can transfer from target cells to lymphocytes to allow the transmission of signals across cell boundaries. Here we show by both FACS and confocal microscopy that human lymphocytes acquire from the cells they scan the inner-membrane protein H-Ras, a G-protein vital for common lymphocyte functions and a prominent participant in human cancer. The transfer was cell contact-dependent and occurred in the context of cell-conjugate formation. Moreover, the acquisition of oncogenic H-RasG12V by natural killer (NK) and T lymphocytes had important biological functions in the adopting lymphocytes: the transferred H-RasG12V induced ERK phosphorylation, increased interferon-γ and tumor necrosis factor-α secretion, enhanced lymphocyte proliferation, and augmented NK-mediated target cell killing. Our findings reveal a novel mode of cell-to-cell communication—allowing lymphocytes to extend the confines of their own proteome—which may moreover play an important role in natural tumor immunity