The ArT\'eMiS wide-field submillimeter camera: preliminary on-sky performances at 350 microns

ArTeMiS is a wide-field submillimeter camera operating at three wavelengths simultaneously (200, 350 and 450 microns). A preliminary version of the instrument equipped with the 350 microns focal plane, has been successfully installed and tested on APEX telescope in Chile during the 2013 and 2014 austral winters. This instrument is developed by CEA (Saclay and Grenoble, France), IAS (France) and University of Manchester (UK) in collaboration with ESO. We introduce the mechanical and optical design, as well as the cryogenics and electronics of the ArTeMiS camera. ArTeMiS detectors are similar to the ones developed for the Herschel PACS photometer but they are adapted to the high optical load encountered at APEX site. Ultimately, ArTeMiS will contain 4 sub-arrays at 200 microns and 2x8 sub-arrays at 350 and 450 microns. We show preliminary lab measurements like the responsivity of the instrument to hot and cold loads illumination and NEP calculation. Details on the on-sky commissioning runs made in 2013 and 2014 at APEX are shown. We used planets (Mars, Saturn, Uranus) to determine the flat-field and to get the flux calibration. A pointing model was established in the first days of the runs. The average relative pointing accuracy is 3 arcsec. The beam at 350 microns has been estimated to be 8.5 arcsec, which is in good agreement with the beam of the 12 m APEX dish. Several observing modes have been tested, like On-The-Fly for beam-maps or large maps, spirals or raster of spirals for compact sources. With this preliminary version of ArTeMiS, we concluded that the mapping speed is already more than 5 times better than the previous 350 microns instrument at APEX. The median NEFD at 350 microns is 600 mJy.s1/2, with best values at 300 mJy.s1/2. The complete instrument with 5760 pixels and optimized settings will be installed during the first half of 2015.Comment: 11 pages, 11 figures. Presented at SPIE Millimeter, Submillimeter, and Far-Infrared Detectors and Instrumentation for Astronomy VII, June 24, 2014. To be published in Proceedings of SPIE Volume 915

Pharmacological targeting of the protein synthesis mTOR/4E-BP1 pathway in cancer-associated fibroblasts abrogates pancreatic tumourchemoresistance

International audiencePancreatic ductal adenocarcinoma (PDAC) is extremely stroma-rich. Cancer-associated fibroblasts (CAFs) secrete proteins that activate survival and promote chemoresistance of cancer cells. Our results demonstrate that CAF secretome-triggered chemoresistance is abolished upon inhibition of the protein synthesis mTOR/4E-BP1 regulatory pathway which we found highly activated in primary cultures of -SMA-positive CAFs, isolated from human PDAC resections. CAFs selectively express the sst1 somatostatin receptor. The SOM230 analogue (Pasireotide) activates the sst1 receptor and inhibits the mTOR/4E-BP1 pathway and the resultant synthesis of secreted proteins including IL-6. Consequently, tumour growth and chemoresistance in nude mice xenografted with pancreatic cancer cells and CAFs, or with pieces of resected human PDACs, are reduced when chemotherapy (gemcitabine) is combined with SOM230 treatment. While gemcitabine alone has marginal effects, SOM230 is permissive to gemcitabine-induced cancer cell apoptosis and acts as an antifibrotic agent. We propose that selective inhibition of CAF protein synthesis with sst1-directed pharmacological compounds represents an anti-stromal-targeted therapy with promising chemosensitization potential

Pacific food systems The role of fish and other aquatic foods for nutrition and health

This report is intended for actors, policymakers and funders concerned with improving food and nutrition security with fisheries as an entry point. It is equally valuable for food system, agricultural, health and nutrition actors who are seeking to protect and enhance the benefits that fish and other aquatic foods provide to a diversity of nutrition goals. This report has been developed from an extensive review of published scientific articles and technical reports, as well as interviews and consultations with 17 experts. It also draws upon insights from the Pacific Food System regional dialogues, which were held in the lead-up to the United Nations Food Systems Summit 2021

The Sharing and Consumption of Dugong and Turtle Meat Outside Torres Strait: management implications and options

[Preamble] This report is associated with Project # 11/6 funded by the Australian Marine Mammal Centre (AMMC), the primary aim of which was to generate information that could be used to support the long-term sustainability of the Torres Strait dugong fishery (already strongly underwritten by the numerous community-based management plans developed during the last 5 years and other research funded by AMMC and NERP). The project was initially conceptualised as an exploration of the sharing and consumption of dugong meat between Torres Strait Islander families. However, it became apparent at the onset of the project (see Section 2.5) that discussions regarding the sharing of dugong meat could not be separated from discussions about the sharing of turtle meat or seafood in general. Dugongs and green turtles are often hunted together and their meat is often shared along with other seafood. Thus, we could not have separated the consumption of these foods without compromising the objectives of the project. This report along with its accompanying documentation thus deals with the sharing of dugong meat, turtle meat and other seafood between Torres Strait Islanders who live in the Torres Strait (TS) and those living on the mainland. Particular attention is, however, paid to issues associated with dugong meat

Socio-Economic Activity and Water Use in Australia's Tropical Rivers: a case study in the Mitchell and Daly River catchments: final report for The Tropical Rivers and Coastal Knowledge Research Consortium

Using an extensive array of data from several different sources, this report describes how water-use input-output models were firstly built and secondly used to explore the way in which Indigenous and Non-Indigenous incomes, employment, and consumptive water demand changes in response to the growth of different industries in two, remote, river catchments in Northern Australia (the Mitchell and the Daly). It finds that:\ud 1) There is an asymmetric divide between Indigenous and Non-Indigenous economic systems in Northern Australia. Given the lack of employment and business opportunities, workplace skills, and the other infrastructure prerequisites for development, local Indigenous people are very unlikely to benefit from the stimulus of any of the north’s existing industries. This situation is likely to persist unless, or until, there is structural change.\ud 2) Some industries may be able to generate significant business income and/or incomes for some householders, but will not necessarily deliver localised benefit in terms of, for example, employment (be it Indigenous or otherwise).\ud 3) Water multipliers differ by orders of magnitude depending upon assumptions made about the numbers of litres of water consumed per dollar output particularly in the Agricultural sector. This clearly highlights the fact that water-saving technologies are vitally important.\ud 4) Both Agriculture and mining are capable of generating significant income flows. But, unlike growth in the government, health or educational sectors, growth in the agricultural sector is associated with significant growth in consumptive water demand. \ud \ud Development strategists and/or policy makers may thus need to explicitly acknowledge both the monetary and non-monetary impacts of different development options (not all of which will be negative), seeking to identify ways in which to exploit synergies and redress tradeoffs. This will allow policy makers to capitalise on opportunities that do not place un-due strain upon the region’s natural resources (water being but one of many important examples). Where tradeoffs exist, policy makers may need to make conscious decisions about what it is they wish to 'develop' (e.g. Regional income or regional employment? Regional income or Australian income?). Moreover, they may need to think about innovative methods of redressing some of the potential problems arising from tradeoffs, ensuring that the methods take account of the structural idiosyncrasies of these small northern economies (i.e. the asymmetric divide noted above)

Motivations for sharing bushmeat with an urban diaspora in indigenous Australia

The hunting and trade of bushmeat is a significant issue. The sharing of marine bushmeat between Australian Torres Strait Islanders and their mainland urban diaspora was documented from a diaspora perspective by collecting quantitative and qualitative data from communities in three mainland cities. Motivations for sharing dugong and turtle meat were almost exclusively cultural and mostly occurred when a diaspora member visited Torres Strait, when Torres Strait Islanders visited their mainland family, during Islander ceremonies, or when goods were exchanged as gifts. Each respondent consumed relatively little dugong and turtle meat (<1–2% of annual meat consumption, or < 1 kg per person per annum). Sharing bushmeat strengthened social capital and reinforced cultural identity. Harnessing the social capital generated from the sharing of bushmeat to engage the urban diaspora in dugong and turtle management activities in the Torres Strait could enhance the legitimacy and effectiveness of such initiatives

Setting the table: indigenous engagement on environmental issues in a politicized context

Environmental issues are often highly politicized. Indigenous peoples may be reluctant to participate in research due to a history of colonization and negative experiences with researchers—described as a "chronic conflict". We present a case where an acute conflict (an event that creates intense mistrust among stakeholders) occurred during a research project. The project studied a contentious issue: the sharing of dugong and turtle meat by Indigenous Australians. The chronic conflict of colonial history and Western research was exacerbated by media coverage of related issues when the project was starting, making participants unwilling to participate in our research. We adapted established Indigenous engagement protocols and — both literally and figuratively — set the table in response to the sociopolitical context of the research. This engagement process enabled us to establish trusting relationships with participants, and could benefit most researchers seeking to engage people, irrespective of Indigeneity or context

The socio-cultural benefits and costs of the traditional hunting of dugongs Dugong dugon and green turtles Chelonia mydas in Torres Strait, Australia

Signatory states of the Convention on Biological Diversity must 'protect and encourage the customary use of biological resources in accordance with traditional cultural practices that are compatible with conservation or sustainable use requirements'. Thus the management of traditional hunting of wildlife must balance the sustainability of target species with the benefits of hunting to traditional communities. Conservation policies usually define the values associated with wild meats in terms of income and nutrition, neglecting a wide range of social and cultural values that are important to traditional hunting communities. We elicited the community-defined benefits and costs associated with the traditional hunting of dugongs Dugong dugon and green turtles Chelonia mydas from communities on two islands in Torres Strait, Australia. We then used cognitive mapping and multidimensional scaling to identify separable groups of benefits (cultural services, provisioning services, and individual benefits) and demonstrate that traditional owners consider the cultural services associated with traditional hunting to be significantly more important than the provisioning services. Understanding these cultural values can inform management actions in accordance with the Convention on Biological Diversity. If communities are unable to hunt, important cultural benefits are foregone. Based on our results, we question the appropriateness of conservation actions focused on prohibiting hunting and providing monetary compensation for the loss of provisioning services only

Developmental and evolutionary comparative analysis of a regulatory landscape in mouse and chicken

Modifications in gene regulation are driving forces in the evolution of organisms. Part of these changes involve cis-regulatory elements (CREs), which contact their target genes through higher-order chromatin structures. However, how such architectures and variations in CREs contribute to transcriptional evolvability remains elusive. We use Hoxd genes as a paradigm for the emergence of regulatory innovations, as many relevant enhancers are located in a regulatory landscape highly conserved in amniotes. Here, we analysed their regulation in murine vibrissae and chicken feather primordia, two skin appendages expressing different Hoxd gene subsets, and compared the regulation of these genes in these appendages with that in the elongation of the posterior trunk. In the two former structures, distinct subsets of Hoxd genes are contacted by different lineage-specific enhancers, probably as a result of using an ancestral chromatin topology as an evolutionary playground, whereas the gene regulation that occurs in the mouse and chicken embryonic trunk partially relies on conserved CREs. A high proportion of these non-coding sequences active in the trunk have functionally diverged between species, suggesting that transcriptional robustness is maintained, despite considerable divergence in enhancer sequences.</p

Context-dependent enhancer function revealed by targeted inter-TAD relocation

The expression of some genes depends on large, adjacent regions of the genome that contain multiple enhancers. These regulatory landscapes frequently align with Topologically Associating Domains (TADs), where they integrate the function of multiple similar enhancers to produce a global, TAD-specific regulation. We asked if an individual enhancer could overcome the influence of one of these landscapes, to drive gene transcription. To test this, we transferred an enhancer from its native location, into a nearby TAD with a related yet different functional specificity. We used the biphasic regulation of Hoxd genes during limb development as a paradigm. These genes are first activated in proximal limb cells by enhancers located in one TAD, which is then silenced when the neighboring TAD activates its enhancers in distal limb cells. We transferred a distal limb enhancer into the proximal limb TAD and found that its new context suppresses its normal distal specificity, even though it is bound by HOX13 transcription factors, which are responsible for the distal activity. This activity can be rescued only when a large portion of the surrounding environment is removed. These results indicate that, at least in some cases, the functioning of enhancer elements is subordinated to the host chromatin context, which can exert a dominant control over its activity.</p