Shrimp by-product hydrolysate induces intestinal myotropic activity in European seabass (Dicentrarchus labrax)

International audienceThe transit time of feed through the digestive tract of fish is very important in fish culture because it can impact fish feeding rates and growth rates, and feed utilization. We identified a myotropic peptide in a shrimp (Litopenaeus vannamei) hydrolysate used in aquaculture fish feeds. It is a new pentapeptide (KNPEQ) cleaved from crustacean hemocyanin, that does not share sequence homology with peptides known to have myotropic activity in fish. We monitored the effect of this hydrolysate in European seabass (Dicentrarchus labrax), (i) in vivo during a feeding trial with low fish meal diets including shrimp hydrolysate, and (ii) in vitro on isolated intestine perfused with hydrolysate extracts. The dietary shrimp hydrolysate accelerated feces emission and led to contractions in perfused intestine. Different steps of purification of shrimp hydrolysate by reverse phase high performance liquid chromatography coupled with intestine in vitro assay were applied in order to purify the myotropic peptide. The sequence of the peptide was determined using both Edman degradation and mass spectrometry fragmentation. We confirmed then in vitro that the KNPEQ-mimetic peptide stimulated the contraction of the intestine in a transient manner at concentrations of 1 and 10 μM, and for >10 h at a concentration of 100 μM

Does equipment change impact blood contamination with irinotecan and its two major metabolites in a centralized cytotoxic pharmacy unit?

International audienceBackground Antineoplastic drugs exposure is a major problem for caregivers’ health. The aim of this study is to assess blood contamination with irinotecan and its two metabolites in a centralized pharmacy unit for cytotoxic drug preparations workers before and after protective equipment changes. Methods The study took place in a university hospital centralized pharmacy unit for cytotoxic drug and was performed in two parts, before (Round 1: R1) and after equipment changes (Round 2: R2). Collection of pharmacy staff blood samples was performed in UHPLC-MS/MS. Plasma and red blood cell irinotecan and its metabolites (SN38; APC) were determined with a validated analytical method detection test. Results A total of 15/36 (41.6%) assays were positive in R1 and 16/72 (22.2%) in R2 with a significant decrease between periods ( P = 0.035). For plasma dosages, no difference between the two periods was found ( P = 0.71); respectively 4/18 (22.2%) assays were positive in R1 and 6/36 (16.6%) in R2. For red blood cells dosages, a significant decrease between periods was found ( P = 0.01); respectively 11/18 (61%) were positive in R1 and 10/36 (27.8%) in R2. Conclusions These dosages make it possible to have the very first evaluation for plasma and red blood cell contamination with irinotecan and its metabolites in the context of equipment changes, both at individual and collective levels. This work would help to protect health workers from the potential risks represented by these molecules, especially by revealing a contamination of workers in order to objectify the results of exposure

Study of Gaseous Compounds Adsorption with a Love Wave Sensor Based on Molecularly Imprinted Polymeric Thin Film

This work exhibits a Love wave sensor combined with molecularly imprinted polymer (MIP). The final aim is for specific detection of colon cancer biomarkers. This paper deals with the validation of a MIP thin film’s coating process, allowing high sensitivity of the resulting microsensor. After a brief description of the acoustic sensor and of the MIP coating process, the functionality of the resulting device is proved with electrical characterization. Deep characterization of the thin film - morphology and print effect - is proposed, based on dynamical responses observed under exposure to different vapors. Experimental results point out a good reproducibility of polymeric films. MIP layers increased responses of sensors to vapors by a factor 3 to 4 compared to bare devices or to devices coated with non-imprinted polymer (NIP). For example, exposure to 4000 mg/m3 (2126 ppm) of ethanol in nitrogen induced a frequency shift of -1.4/-0.4 kHz with MIP/NIP-coated sensor, respectively

Love Wave Sensor Based on Thin Film Molecularly Imprinted Polymer: Study of VOCs Adsorption

ABSTRACT The overall objective of this work is to develop and to validate a quantitative, non-invasive diagnosis tool to monitor the efficiency of colorectal cancer chemotherapy. This paper deals with the validation of a molecularly imprinted polymer (MIP) thin film's coating process, allowing high sensitivity of the resulting microsensor. After description of the Love wave sensor and of the MIP coating process, the functionality of the resulting device is proved with electrical characterization. Deep characterization of the thin film -morphology and print effect -is proposed, based on dynamical responses observed under exposure to different vapors. Results point out a good reproducibility of polymeric films. MIP layers increased responses of sensors to vapors by a factor 3 to 4 compared to bare devices or to devices coated with non-imprinted polymer (NIP). For example, exposure to 4000mg/m 3 (2126ppm) of ethanol in nitrogen induced a frequency shift of -1.4/-0.4kHz with MIP/NIP-coated sensor, respectively

Rapid measurement of 13C-enrichment of acetic, propionic and butyric acids in plasma with solid phase microextraction coupled to gas chromatography–mass spectrometry

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Urban air pollution fine particulate matter exposure: Biomarkers of carcinogenesis modulated by people ageing

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Urban air pollution fine particulate matter exposure: Biomarkers of carcinogenesis modulated by people ageing

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