New records of Tephritidae (Diptera) from Great Smoky Mountains National Park - 2

Thirty additional species of tephritid flies (Diptera: Tephritidae) from Great Smoky Mountains National Park (GSMNP), including historical records, are presented together with information on host(s), if known, distributions, and life histories. This brings the total number of tephritid flies recorded from GSMNP to 46

Scaling of Incidence Variations With Inlet Distortion for a Transonic Axial Compressor

This paper numerically explores the manner in which blade row inlet incidence variation scales with various distortion patterns and intensities. The objectives are to (1) identify the most appropriate parameter whose circumferential variation can be used to assess scaling relationships of a transonic compressor and (2) use this parameter to evaluate two types of non-uniform inflow patterns, vertically stratified total pressure distortions and radially stratified total enthalpy and total pressure distortions. A body force model of the blade rows is employed to reduce computational cost; the approach has been shown to capture distortion transfer and to be able to predict upstream flow redistribution with inlet distortion. Diffusion factor is shown to be an inadequate proxy for streamline loss generation in non-uniform flow, leading to the choice of incidence angle variations as the metric for which we assess scaling relationships. Posteriori scaling of circumferential flow angle variation based on the maximum incidence excursion for varying distortion intensity yields an accurate method for prediction of the impact for other distortion intensities; linear regression of the maximum variation in incidence around the annulus as a function of distortion intensity had R2 \u3e 0.97 for all spanwise locations examined in both the rotor and stator for both vertically and radially stratified distortions. However, changes to far upstream distortion shape yield highly non-linear incidence variation scaling; the results suggest that the pitchwise gradients of far upstream total pressure govern the degree of linearity for incidence variation scaling

Fan Performance Scaling With Inlet Distortions

Applications such as boundary-layer-ingesting fans, and compressors in turboprop engines require continuous operation with distorted inflow. A low-speed axial fan with incompressible flow is studied in this paper. The objectives are to (1) identify the physical mechanisms which govern the fan response to inflow distortions and (2) determine how fan performance scales as the type and severity of inlet distortion varies at the design flow coefficient. A distributed source term approach to modeling the rotor and stator blade rows is used in numerical simulations in this paper. The model does not include viscous losses so that changes in diffusion factor are the primary focus. Distortions in stagnation pressure and temperature as well as swirl are considered. The key findings are that unless sharp pitchwise gradients in the diffusion response, strong radial flows, or very large distortion magnitudes are present, the response of the blade rows for strong distortions can be predicted by scaling up the response to a weaker distortion. In addition, the response to distortions which are composed of non-uniformities in several inlet quantities can be predicted by summing up the responses to the constituent distortions

The co-development of friends’ delinquency with adolescents’ delinquency and short-term mindsets:The moderating role of co-offending

The companions in crime hypothesis suggests that co-offending moderates the link between peer delinquency and adolescent delinquency. However, this hypothesis has rarely been investigated longitudinally. Hence, this study investigated the co-development of friends’ delinquency and adolescents’ delinquency, as well as the co-development of friends’ delinquency and short-term mindsets (impulsivity and lack of school future orientation). Whether this co-development is stronger when adolescents engage in co-offending was also investigated. Three data waves with two year lags from an ethnically-diverse adolescent sample (at wave 1: N = 1365; 48.6% female; M(age) = 13.67; age range = 12.33–15.09 years) in Switzerland were used. The results from parallel process latent growth modeling showed that the co-development between friends’ delinquency and adolescents’ delinquency was stronger when adolescents engaged in co-offending. Thus co-offending likely provides direct access to a setting in which adolescents continue to model the delinquency they learned with their peers

Accelerated Turnover of Taste Bud Cells in Mice Deficient for the Cyclin-Dependent Kinase Inhibitor p27\u3csup\u3ekip1\u3c/sup\u3e

Background: Mammalian taste buds contain several specialized cell types that coordinately respond to tastants and communicate with sensory nerves. While it has long been appreciated that these cells undergo continual turnover, little is known concerning how adequate numbers of cells are generated and maintained. The cyclin-dependent kinase inhibitor p27Kip1 has been shown to influence cell number in several developing tissues, by coordinating cell cycle exit during cell differentiation. Here, we investigated its involvement in the control of taste cell replacement by examining adult mice with targeted ablation of the p27Kip1 gene.Results: Histological and morphometric analyses of fungiform and circumvallate taste buds reveal no structural differences between wild-type and p27Kip1-null mice. However, when examined in functional assays, mutants show substantial proliferative changes. In BrdU incorporation experiments, more S-phase-labeled precursors appear within circumvallate taste buds at 1 day post-injection, the earliest time point examined. After 1 week, twice as many labeled intragemmal cells are present, but numbers return to wild-type levels by 2 weeks. Mutant taste buds also contain more TUNEL-labeled cells and 50% more apoptotic bodies than wild-type controls. In normal mice, p27 Kip1 is evident in a subset of receptor and presynaptic taste cells beginning about 3 days post-injection, correlating with the onset of taste cell maturation. Loss of gene function, however, does not alter the proportions of distinct immunohistochemically-identified cell types.Conclusions: p27Kip1 participates in taste cell replacement by regulating the number of precursor cells available for entry into taste buds. This is consistent with a role for the protein in timing cell cycle withdrawal in progenitor cells. The equivalence of mutant and wild-type taste buds with regard to cell number, cell types and general structure contrasts with the hyperplasia and tissue disruption seen in certain developing p27Kip1-null sensory organs, and may reflect a compensatory capability inherent in the regenerative taste system

A Non-Canonical Role for p27\u3csup\u3eKip1\u3c/sup\u3e in Restricting Proliferation of Corneal Endothelial Cells During Development

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The cell cycle regulator p27Kip1 is a critical factor controlling cell number in many lineages. While its anti-proliferative effects are well-established, the extent to which this is a result of its function as a cyclin-dependent kinase (CDK) inhibitor or through other known molecular interactions is not clear. To genetically dissect its role in the developing corneal endothelium, we examined mice harboring two loss-of-function alleles, a null allele (p27−) that abrogates all protein function and a knockin allele (p27CK−) that targets only its interaction with cyclins and CDKs. Whole-animal mutants, in which all cells are either homozygous knockout or knockin, exhibit identical proliferative increases (~0.6-fold) compared with wild-type tissues. On the other hand, use of mosaic analysis with double markers (MADM) to produce infrequently-occurring clones of wild-type and mutant cells within the same tissue environment uncovers a roughly three- and six-fold expansion of individual p27CK−/CK− and p27−/− cells, respectively. Mosaicism also reveals distinct migration phenotypes, with p27−/− cells being highly restricted to their site of production and p27CK−/CK− cells more widely scattered within the endothelium. Using a density-based clustering algorithm to quantify dispersal of MADM-generated clones, a four-fold difference in aggregation is seen between the two types of mutant cells. Overall, our analysis reveals that, in developing mouse corneal endothelium, p27 regulates cell number by acting cell autonomously, both through its interactions with cyclins and CDKs and through a cyclin-CDK-independent mechanism(s). Combined with its parallel influence on cell motility, it constitutes a potent multi-functional effector mechanism with major impact on tissue organization

Trait urgency and gambling problems in young people by age: The mediating role of decision-making processes

Although the personality trait of urgency has been linked to problem gambling, less is known about psychological mechanisms that mediate the relationship between urgency and problem gambling. One individual variable of potential relevance to impulsivity and addictive disorders is age. The aims of this study were to examine: (i) a theoretical model associating urgency and gambling problems, (ii) the mediating effects of decision-making processes (operationalized as preference for small/immediate rewards and lower levels of deliberative decision-making); and (iii) age differences in these relationships. Participants comprised 986 students (64% male; mean age=19.51 years; SD=2.30) divided into three groups: 16-17 years, 18-21 years, and 22-25 years. All participants completed measures of urgency, problem gambling, and a delay-discounting questionnaire involving choices between a smaller amount of money received immediately and a larger amount of money received later. Participants were also asked to reflect on their decision-making process. Compared to those aged 16-17 years and 22-25 years, participants aged 18-21 years had a higher level of gambling problems and decreased scores on lower levels of deliberative decision-making. Higher levels of urgency were associated with higher levels of gambling problems. The association was mediated by a lower level of deliberative decision-making and preference for an immediate/small reward. A distinct pathway was observed for lower levels of deliberative decision-making. Young people who tend to act rashly in response to extreme moods, had lower levels of deliberative decision-making, that in turn were positively related to gambling problems. This study highlights unique decision-making pathways through which urgency trait may operate, suggesting that those developing prevention and/or treatment strategies may want to consider the model’s variables, including urgency, delay discounting, and deliberative decision-making