The effects of dietary fiber level on nutrient digestibility in growing pigs

The objective of this study was to investigate the effects of total dietary fiber level on nutrient digestibility and the relationship between apparent total tract digestibility of total dietary fiber, and soluble dietary fiber, insoluble dietary fiber and available energy. Sugar beet pulp was as the only fiber source. The experiment was designed as a 6 × 6 Latin square with an adaptation period of 7 d followed by a 5-d total collection of feces and urine. Feed intake tended to decrease (P =0.10) as total dietary fiber level increased. The apparent total tract digestibility of dry matter, crude protein and gross energy decreased (P <0.01) when total dietary fiber increased but the digestibility of soluble dietary fiber and insoluble dietary fiber increased (P <0.01). The digestible energy and metabolizable energy content of diets decreased (P <0.01) as the total dietary fiber increased

Effect of conjugated linoleic acid on inhibition of prolyl hydroxylase 1 in hearts of mice

<p>Abstract</p> <p>Background</p> <p>Results from different trails have provided evidence of protective effects of <it>cis-</it>9,<it>trans</it>-11-conjugated linoleic acid (CLA) on cardiovascular diseases. But the inhibition of prolyl hydroxylase 1 (PHD1) associated with induction of hypoxia inducible factors (HIFs) by CLA in these protective effects has never been reported before. The objective of this study was to evaluate if the two predominant <it>cis-</it>9,<it>trans</it>-11 (c9, t11), <it>trans</it>-10,<it>cis</it>-12 (t10, c12) CLA isomers and mixture of these two isomers can inhibit PHD1 with induction of HIFs in myocardium in mice and subsequent effects on myocardium metabolism.</p> <p>Results</p> <p>CLA mixture and c9, t11 CLA inhibited PHD1 protein expression and increased the levels of protein and mRNA in HIF-2α in myocardium in mice. Meanwhile, CLA mixture and c9, t11 CLA also elevated the expression of HIF related transcriptional factors like PDK4 and PPARα. The reprogramming of basal metabolism in myocardium in mice was shown on increasing of GLUT4 gene expression by c9, t11 CLA supplemented group. UCP2 was increased by CLA mixture and c9, t11 CLA for attenuating production of ROS.</p> <p>Conclusion</p> <p>CLA mixture and c9, t11 CLA could inhibit PHD1 and induce HIF-2α in myocardium in mice, which is associated with upregulation of PDK4 by activation of PPARα. This process also implies a reprogramming of basal metabolism and oxidative damage protection in myocardium in mice. All the effects shown in hearts of mice are due to c9, t11 CLA but not t10, c12 CLA.</p

Homologous illegitimate random integration of foreign DNA into the X chromosome of a transgenic mouse line

<p>Abstract</p> <p>Background</p> <p>It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the <it>fad2 </it>gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integration.</p> <p>Results</p> <p>We confirmed that one <it>fad2 </it>line had a sole integration site on the X chromosome according to the inheritance patterns. Mapping of insertion sequences with thermal asymmetric interlaced and conventional PCR revealed that the foreign DNA was inserted into the XC1 region of the X chromosome by a homologous illegitimate replacement of an entire 45,556-bp endogenous genomic region, including the ovarian granulosa cell tumourigenesis-4 allele. For 5' and 3' junction sequences, there were very short (3-7 bp) common sequences in the AT-rich domains, which may mediate the recognition of the homologous arms between the transgene and the host genome. In addition, analysis of gene transcription indicated that the transgene was expressed in all tested <it>fad2 </it>tissues and that its transcription level in homozygous female tissues was about twice as high as in the heterozygous female (p < 0.05).</p> <p>Conclusions</p> <p>Taken together, the results indicated that the foreign <it>fad2 </it>behaved like an X-linked gene and that foreign DNA molecules were inserted into the eukaryotic genome through a homologous illegitimate random integration.</p

RVM Classification of Hyperspectral Images Based on Wavelet Kernel Non-negative Matrix Fractorization

A novel kernel framework for hyperspectral image classification based on relevance vector machine (RVM) is presented in this paper. The new feature extraction algorithm based on Mexican hat wavelet kernel non-negative matrix factorization (WKNMF) for hyperspectral remote sensing images is proposed. By using the feature of multi-resolution analysis, the new method of nonlinear mapping capability based on kernel NMF can be improved. The new classification framework of hyperspectral image data combined with the novel WKNMF and RVM. The simulation experimental results on HYDICE and AVIRIS data sets are both show that the classification accuracy of proposed method compared with other experiment methods even can be improved over 10% in some cases and the classification precision of small sample data area can be improved effectively

Spectroscopic Evidence of Type II Weyl Semimetal State in WTe2

Quantum topological materials, exemplified by topological insulators, three-dimensional Dirac semimetals and Weyl semimetals, have attracted much attention recently because of their unique electronic structure and physical properties. Very lately it is proposed that the three-dimensional Weyl semimetals can be further classified into two types. In the type I Weyl semimetals, a topologically protected linear crossing of two bands, i.e., a Weyl point, occurs at the Fermi level resulting in a point-like Fermi surface. In the type II Weyl semimetals, the Weyl point emerges from a contact of an electron and a hole pocket at the boundary resulting in a highly tilted Weyl cone. In type II Weyl semimetals, the Lorentz invariance is violated and a fundamentally new kind of Weyl Fermions is produced that leads to new physical properties. WTe2 is interesting because it exhibits anomalously large magnetoresistance. It has ignited a new excitement because it is proposed to be the first candidate of realizing type II Weyl Fermions. Here we report our angle-resolved photoemission (ARPES) evidence on identifying the type II Weyl Fermion state in WTe2. By utilizing our latest generation laser-based ARPES system with superior energy and momentum resolutions, we have revealed a full picture on the electronic structure of WTe2. Clear surface state has been identified and its connection with the bulk electronic states in the momentum and energy space shows a good agreement with the calculated band structures with the type II Weyl states. Our results provide spectroscopic evidence on the observation of type II Weyl states in WTe2. It has laid a foundation for further exploration of novel phenomena and physical properties in the type II Weyl semimetals.Comment: 16 Pages, 4 Figure