Interpreting Neuroendocrine Hormones, Corticosterone, and Blood Glucose to Assess the Wellbeing of Anesthetized Rats during Euthanasia

Current recommendations for assessing animal wellbeing during euthanasia suggest that measuring neuroendocrine hormones-such as ACTH, noradrenaline, and adrenaline-is preferable to measuring corticosterone and blood glucose because of the sensitivity of neuroendocrine hormones to the acute stress associated with rapid methods of euthanasia. However, theseneuroendocrine hormones can be stimulated in ways that confound interpretation of welfare assessment in euthanasia studies.Although this property does not negate the usefulness of neuroendocrine hormones as tools of assessment, it is importantto differentiate the stress associated with the induction of anesthesia before the loss of consciousness (an animal wellbeingconcern) with the physiologic responses that occur after the loss of consciousness (not an animal wellbeing concern). In thisstudy, rats were anesthetized by using a ketamine-xylazine combination. Once the rats achieved a surgical plane of anesthesia,they were exposed to O2, CO2, or isoflurane, followed by terminal blood collection to assess concentrations of ACTH,noradrenaline, corticosterone, and blood glucose. Compared with animals exposed to O2 or isoflurane, rats exposed to CO2had significant increases in their serum concentrations of ACTH and noradrenaline, but blood glucose and corticosteronedid not differ between groups. These findings indicate that noradrenaline and ACTH should be used with caution to assessanimal wellbeing when the method of euthanasia might confound that assessment

Home Cage Compared with Induction Chamber for Euthanasia of Laboratory Rats

This study compared behavioral and physiologic changes in Sprague-Dawley and Brown Norway rats that were euthanizedby using a 30% volume displacement rate of CO2 in either their home cage or an induction chamber; rats euthanized in thehome cage were hypothesized to demonstrate a higher level of animal wellbeing. No significant differences were detectedin the physiologic responses to home cage versus induction chamber euthanasia groups. A few strain-related behavioraldifferences occurred. The number of digs per second was higher in Brown Norway compared with Sprague-Dawley rats when in the home cage, where a digging substrate was present. Rearing frequency was higher in both Brown Norway and Sprague-Dawley rats in the induction chamber compared with the home cage. This study demonstrated that although strainspecific differences were associated with the process of euthanasia, there were no significant differences between the treatment groups of home cage compared with induction chamber. This finding suggests that-from the perspective of a rat-either the home cage or an induction chamber can be used for euthanasia, with likely extension of this conclusion to use of either method to the induction of anesthesia

Memory and Justice: Confronting Past Atrocity and Human Rights Abuse

This report examines the development of the movement to deal with the past from approximately 1983 to 2008 with an emphasis on the impact of Ford Foundation support, particularly from the Andean Region and Southern Cone office since the early 1990s. How has this support to various organizations mattered? How has it made a difference? Moving beyond the contribution of the Ford Foundation, the report also examines the ways in which dealing with the past has become characterized by a proliferation of activities and initiatives, as well as the creation of new institutions.The report draws on more than a dozen interviews, written correspondence with a selection of key actors, Ford Foundation grant files, an earlier consultancy report written by Professor Peter Winn, and eight commissioned papers on dealing with the past in specific countries or areas of interest

First Class: Pioneering Students at San José State University’s School of Library and Information Science, 1928-1940

This article examines the backgrounds, education, and careers of the first group of students in San José State University’s School of Library and Information Science. It finds that the 1928-1929 cohort were typical of the students attending teacher’s colleges in the early 1900s and represented the first generation of women pursuing higher education and professional careers following the passage of the 19th amendment in 1920. The study also explores the challenges working women faced during the 1930s, particularly the Great Depression’s impact California librarians

Wellbeing of Alcohol-preferring Rats Euthanized with Carbon Dioxide at Very Low and Low Volume Displacement Rates

The 2013 AVMA Guidelines on Euthanasia recommend the use of very-low or low flow rates of 100% carbon dioxide to euthanize small rodents. Although inhalation of high concentrations of carbon dioxide are generally recognized as painful in humans, whether the use of these low-flow methods of euthanasia increase potential distress for rats is unclear. This study compared physiologic and behavioral markers of animal wellbeing for rats euthanized by using 10% volume displacement per minute (VD/min), 30% VD/min, and 70% VD/min of 100% carbon dioxide. Rats were recorded during euthanasia for subsequent behavioral scoring, and blood samples were taken after euthanasia for assessment of blood glucose and serum corticosterone levels. In this study, rats euthanized with 10% or 30% VD/min of 100% carbon dioxide demonstrated increases in various behaviors, such as rearing and standing, concurrent with increases in serum corticosterone. Rats euthanized with 70% VD/min of 100% carbon dioxide did not exhibit these changes. The results suggest that a euthanasia method of 70% VD/min of 100% carbon dioxide may minimize potential pain and distress and thus be more humane for rats, as compared with very-low- and low-flow methods of carbon dioxide euthanasia

“Me Too”: Epistemic Injustice and the Struggle for Recognition

Congdon (2017), Giladi (2018), and McConkey (2004) challenge feminist epistemologists and recognition theorists to come together to analyze epistemic injustice. I take up this challenge by highlighting the failure of recognition in cases of testimonial and hermeneutical injustice experienced by victims of sexual harassment and sexual assault. I offer the #MeToo movement as a case study to demonstrate how the process of mutual recognition makes visible and helps overcome the epistemic injustice suffered by victims of sexual harassment and sexual assault. I argue that in declaring “me too,” the epistemic subject emerges in the context of a polyphonic symphony of victims claiming their status as agents who are able to make sense of their own social experiences and able to convey their knowledge to others

Constraints to school effectiveness: what prevents poor schools from delivering results?

The poor state of quality education in South Africa is confirmed by the weak performance of South African students on international tests, even when compared to countries with comparatively poorer education systems. This paper aims to shed light on this issue through the use of the PIRLS 2006 dataset and education production function techniques. A unique feature of this dataset is that schools were able to choose the language in which the test was conducted. This provided a proxy for former school department, a feature that has not been captured in international survey datasets. A clear distinction between the historically black and the historically white, coloured and Indian school systems is needed in order to identify the different data generating processes at work. The regression model results reveal that family and student characteristics are undoubtedly important for performance within both school samples. At the level of the school, quite divergent school factors and classroom processes were found to have significant impacts on student performance across the two school systems. It is concluded that a lack of enabling conditions such as effective leadership, flexibility and autonomy, and a capable teaching force may contribute to certain school and classroom processes not playing a significant role in determining performance in the less affluent black school system.South Africa, Education, Education production function, Educational Achievement, Educational Inequality

Effects of Music Enrichment on Individually Housed Male New Zealand White Rabbits

The beneficial effect of music has been demonstrated in many species. Although commercially available music CDs intended for use with rabbits are available, these enrichments have not been critically evaluated to determine whether they reduce distress. In this study, we used an aging colony of male rabbits to evaluate how the provision of music enrichment affected the wellbeing of the colony. After 6 mo of music enrichment, the rabbits in the colony demonstrated a significant decrease in fecal cortisol, suggesting that their stress was reduced. Six months after removal of the music enrichment, the rabbits demonstrated a significant increase in fecal cortisol and the heterophil:lymphocyte ratio, suggesting that they werestressed. These findings suggest that music enrichment with a commercially available music discs for rabbits can be used toimprove the wellbeing of animals used in biomedical research

Integrating testing techniques through process programming

Integration of multiple testing techniques is required to demonstrate high quality of software. Technique integration has three basic goals: incremental testing capabilities, extensive error detection, and cost-effective application. We are experimenting with the use of process programming as a mechanism of integrating testing techniques. Having set out to integrate DATA FLOW testing and RELAY, we proposed synergistic use of these techniques to achieve all three goals. We developed a testing process program much as we would develop a software product from requirements through design to implementation and evaluation. We found process programming to be effective for explicitly integrating the techniques and achieving the desired synergism. Used in this way, process programming also mitigates many of the other problems that plague testing in the software development process