The use of diagrams in project representation for architecture publications : the Domus case

Orientador: Daniel de Carvalho MoreiraDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Civil, Arquitetura e UrbanismoResumo: O diagrama é considerado importante ferramenta de pensamento e de expressão gráfica para os arquitetos, principalmente na atualidade, em que ganham visibilidade ainda maior em decorrência do aumento da sistematização dos processos projetuais e ampliação da divulgação midiática da arquitetura. A presente pesquisa tem por finalidade proporcionar maior familiaridade com seu uso no panorama das publicações arquitetônicas, ainda um dos principais meios de divulgação da produção da área. A primeira parte do trabalho consiste no levantamento e estudo do material já produzido sobre o tema. Já a segunda parte explora o uso dessa técnica gráfica através da utilização de um procedimento de análise, baseado na sistematização e organização de classificações tipológicas desenvolvidos por autores selecionados. A aplicação foi realizada em uma amostragem de diagramas presentes no periódico de arquitetura italiano Domus. O recorte foi feito através da coletânea realizada pela Editora alemã Taschen, uma antologia histórica da revista em 12 volumes, com destaques selecionados dos anos de 1928 a 1999. Como resultado, obteve-se um mapeamento dos principais comportamentos dos tipos de diagramas levantados, o que auxiliará na compreensão e na tomada de decisões quanto à novos diagramasAbstract: Regarding diagrams as important thinking tools and graphic expression for architects, especially nowadays, diagrams gain even more visibility due to the increase of projects processes systematization and the architecture media diffusion expansion. This research aims to provide greater familiarity with the use of diagrams in the panorama of architectural publications, still one of the main forms of publicizing the area production. The first part of this work consists of survey and study of the material already produced about diagrams. The second part explores the application of this graphic technique through the use of analysis procedures, with reference to typological classification, systematization and organization developed by selected authors. The application was carried out in a selection of diagrams present in the italian architectural periodical Domus. The selection was done over the German Taschen Publishing collection, a 12-volume historical anthology, with selected highlights from the years 1928 to 1999. As a result, the research obtained a mapping of the types of diagrams main behaviors surveyed, which will encourage the understanding and decision-making about new diagramsMestradoArquitetura, Tecnologia e CidadeMestra em Arquitetura, Tecnologia e Cidad

Immunohistochemical expression of SOX9 protein in immature, mature, and neoplastic canine Sertoli cells

Sex-determining region Y box9 gene (SOX9) protein plays a pivotal role in male sexual development. It regulates the transcription of the anti-Müllerian hormone gene promoting development of testis cords, multiplication, and maturation of Sertoli cells (SCs) and maintenance of spermatogenesis in adult testis. The immunohistochemical expression of SOX9 in normal testes has been reported in humans, mice, and rats. The present study aimed to investigate the expression of SOX9 in canine SCs during testicular maturation and neoplastic transformation. Canine testicular samples derived from three fetuses, four newborns, four prepubertal puppies, five adult dogs, 31 Sertoli cell tumors (SCTs) (one metastasizing), and five Leydig cell tumors (LCTs) were selected from departmental archive and tested immunohistochemically with a polyclonal antibody against SOX9 (1:150). All SCs from fetal, neonatal, and adult testes had a strong and exclusively nuclear labeling for SOX9. In SCs from prepubertal testes, SOX9 staining was highly variable with one negative sample (one of four), two samples with exclusively nuclear staining (two of four), and one with both nuclear and cytoplasmic labeling (one of four). Leydig cells (LCs) and LCTs were always negative. All 31 SCTs were positive for SOX9. The expression of SOX9 was nuclear, nuclear and cytoplasmic, and exclusively cytoplasmic in 18 of 31, 11 of 31, and two of 31 SCTs, respectively. This first report on the immunohistochemical expression of SOX9 in canine testes reports that in normal SCs from fetal, neonatal, and adult testes SOX9 labeled the nucleus, as in humans and laboratory animals. The cytoplasmic labeling observed in one prepubertal pairs of testes and in 11 SCTs could reflect SC immaturity or dedifferentiation, paralleling results observed in rat testes. The expression of SOX9 in SCs and SCTs and its absence in LCs and LCTs suggests that SOX9 is a reliable diagnostic marker for both normal and neoplastic SCs