Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species

BACKGROUND: Flow cytometry is a powerful tool for the multiparameter analysis of leukocyte subsets on the single cell level. Recent advances have greatly increased the number of fluorochrome-labeled antibodies in flow cytometry. In particular, an increase in available fluorochromes with distinct excitation and emission spectra combined with novel multicolor flow cytometers with several lasers have enhanced the generation of multidimensional expression data for leukocytes and other cell types. However, these advances have mainly benefited the analysis of human or mouse cell samples given the lack of reagents for most animal species. The flow cytometric analysis of important veterinary, agricultural, wildlife, and other animal species is still hampered by several technical limitations, even though animal species other than the mouse can serve as more accurate models of specific human physiology and diseases. RESULTS: Here we present time-tested approaches that our laboratory regularly uses in the multiparameter flow cytometric analysis of ovine leukocytes. The discussed approaches will be applicable to the analysis of cells from most animal species and include direct modification of antibodies by covalent conjugation or Fc-directed labeling (Zenon™ technology), labeled secondary antibodies and other second step reagents, labeled receptor ligands, and antibodies with species cross-reactivity. CONCLUSIONS: Using refined technical approaches, the number of parameters analyzed by flow cytometry per cell sample can be greatly increased, enabling multidimensional analysis of rare samples and giving critical insight into veterinary and other less commonly analyzed species. By maximizing information from each cell sample, multicolor flow cytometry can reduce the required number of animals used in a study

Perspective acceleration and gravitational redshift. Measuring masses of individual white dwarfs using Gaia + SIM astrometry

According to current plans, the SIM/NASA mission will be launched just after the end of operations for the Gaia/ESA mission. This is a new situation which enables long term astrometric projects that could not be achieved by either mission alone. Using the well-known perspective acceleration effect on astrometric measurements, the true heliocentric radial velocity of a nearby star can be measured with great precision if the time baseline of the astrometric measurements is long enough. Since white dwarfs are compact objects, the gravitational redshift can be quite large (40-80 km/s), and is the predominant source of any shift in wavelength. The mismatch of the true radial velocity with the spectroscopic shift thus leads to a direct measure of the Mass--Radius relation for such objects. Using available catalog information about the known nearby white dwarfs, we estimate how many masses/gravitational redshift measurements can be obtained with an accuracy better than 2%. Nearby white dwarfs are relatively faint objects (10 < V < 15), which can be easily observed by both missions. We also briefly discuss how the presence of a long period planet can mask the astrometric signal of perspective acceleration.Comment: 3 pages, 2 Figures. Proceedings of the IAU Symposium 261 : Relativity in Fundamental Astronomy. 27 April - 1 May 2009, Virginia Beach, VA, USA. refereed and accepted versio

The Survival Rate of Ejected Terrestrial Planets with Moons

During planet formation, a gas giant will interact with smaller protoplanets that stray within its sphere of gravitational influence. We investigate the outcome of interactions between gas giants and terrestrial-sized protoplanets with lunar-sized companions. An interaction between a giant planet and a protoplanet binary may have one of several consequences, including the delivery of volatiles to the inner system, the capture of retrograde moons by the giant planet, and the ejection of one or both of the protoplanets. We show that an interesting fraction of terrestrial-sized planets with lunar sized companions will likely be ejected into interstellar space with the companion bound to the planet. The companion provides an additional source of heating for the planet from tidal dissipation of orbital and spin angular momentum. This heat flux typically is larger than the current radiogenic heating of the Earth for up to the first few hundred million years of evolution. In combination with an atmosphere of sufficient thickness and composition, the heating can provide the conditions necesary for liquid water to persist on the surface of the terrestrial mass planet, making it a potential site for life. We also determine the possibility for directly detecting such systems through all-sky infrared surveys or microlensing surveys. Microlensing surveys in particular will directly measure the frequency of this phenomenon.Comment: 4 pages, 2 figures, Accepted to ApJ

Color Gradients Detected in the HD 15115 Circumstellar Disk

We report HST/NICMOS coronagraphic images of the HD 15115 circumstellar disk at 1.1\micron. We find a similar morphology to that seen in the visible and at H band--an edge-on disk that is asymmetric in surface brightness. Several aspects of the 1.1\micron data are different, highlighting the need for multi-wavelength images of each circumstellar disk. We find a flattening to the western surface brightness profile at 1.1\micron interior to 2\arcsec (90 AU) and a warp in the western half of the disk. We measure the surface brightness profiles of the two disk lobes and create a measure of the dust scattering efficiency between 0.55-1.65\micron at 1\arcsec, 2\arcsec, and 3\arcsec. At 2\arcsec the western lobe has a neutral spectrum up to 1.1\micron and a strong absorption or blue spectrum $>$1.1\micron, while a blue trend is seen in the eastern lobe. At 1\arcsec the disk has a red F110W-H color in both lobes.Comment: 4 pages, 4 figures, emulateapj; accepted to ApJ

The WIRED Survey. IV. New Dust Disks from the McCook & Sion White Dwarf Catalog

We have compiled photometric data from the Wide-field Infrared Survey Explorer All Sky Survey and other archival sources for the more than 2200 objects in the original McCook & Sion Catalog of Spectroscopically Identified White Dwarfs. We applied color-selection criteria to identify 28 targets whose infrared spectral energy distributions depart from the expectation for the white dwarf photosphere alone. Seven of these are previously known white dwarfs with circumstellar dust disks, five are known central stars of planetary nebulae, and six were excluded for being known binaries or having possible contamination of their infrared photometry. We fit white dwarf models to the spectral energy distributions of the remaining ten targets, and find seven new candidates with infrared excess suggesting the presence of a circumstellar dust disk. We compare the model dust disk properties for these new candidates with a comprehensive compilation of previously published parameters for known white dwarfs with dust disks. It is possible that the current census of white dwarfs with dust disks that produce an excess detectable at K-band and shorter wavelengths is close to complete for the entire sample of known WDs to the detection limits of existing near-IR all-sky surveys. The white dwarf dust disk candidates now being found using longer wavelength infrared data are drawn from a previously underrepresented region of parameter space, in which the dust disks are overall cooler, narrower in radial extent, and/or contain fewer emitting grains.Comment: accepted for publication in The Astrophysical Journal; 34 pages, 5 figures, 5 tables; added missing reference in Section 2 (p. 7

Cool Customers in the Stellar Graveyard IV: Spitzer Search for Mid-IR excesses Around Five DAs

Hydrogen atmosphere white dwarfs with metal lines, so-called DAZs, require external accretion of material to explain the presence of weak metal line absorption in their photospheres. The source of this material is currently unknown, but could come from the interstellar medium, unseen companions, or relic planetesimals from asteroid belt or Kuiper belt analogues. Accurate mid-infrared photometry of these white dwarfs provide additional information to solve the mystery of this accretion and to look for evidence of planetary systems that have survived post main sequence evolution. We present {\em Spitzer} IRAC photometry accurate to $\sim$3% for four DAZs and one DA with circumstellar absorption lines in the UV. We search for excesses due to unseen companions or circumstellar dust disks. We use {\em Hubble Space Telescope} NICMOS imaging of these white dwarfs to gauge the level of background contamination to our targets as well as rule out common proper motion companions to WD 1620-391. All of our targets show no excesses due to companions $>$20 M$_{J}$, ruling out all but very low mass companions to these white dwarfs at all separations. No excesses due to circumstellar disks are observed, and we place limits on what types of disks may still be present.Comment: 18 pages, 8 figures, Accepted to A

Optical Coronagraphic Spectroscopy of AU Mic: Evidence of Time Variable Colors?

We present coronagraphic long slit spectra of AU Mic's debris disk taken with the STIS instrument aboard the Hubble Space Telescope (HST). Our spectra are the first spatially resolved, scattered light spectra of the system's disk, which we detect at projected distances between approximately 10 and 45 AU. Our spectra cover a wavelength range between 5200 and 10200 angstroms. We find that the color of AU Mic's debris disk is bluest at small (12-35 AU) projected separations. These results both confirm and quantify the findings qualitatively noted by Krist et al. (2005), and are different than IR observations that suggested a uniform blue or gray color as a function of projected separation in this region of the disk. Unlike previous literature that reported the color of AU Mic's disk became increasingly more blue as a function of projected separation beyond approximately 30 AU, we find the disk's optical color between 35-45 AU to be uniformly blue on the southeast side of the disk and decreasingly blue on the northwest side. We note that this apparent change in disk color at larger projected separations coincides with several fast, outward moving "features" that are passing through this region of the southeast side of the disk. We speculate that these phenomenon might be related, and that the fast moving features could be changing the localized distribution of sub-micron sized grains as they pass by, thereby reducing the blue color of the disk in the process. We encourage follow-up optical spectroscopic observations of the AU Mic to both confirm this result, and search for further modifications of the disk color caused by additional fast moving features propagating through the disk.Comment: Accepted by AJ, 13 pages, 8 figures, 1 tabl