Using the Fishes of Texas Project Databases and Recent Collections to Detect Range Expansions by Four Fish Species on the Lower Coastal Plain of Texas

The Fishes of Texas project online database is a large, freely available quality controlled fish occurrence database of museum vouchered specimens. We used data from it, the same project’s separate database of occurrences extracted from published literature and our own recent survey data to examine range stability for four fish species inhabiting the Texas Lower Coastal Plain: Fundulus chrysotus, Fundulus jenkinsi, Heterandria formosa and Poecilia formosa. A weakness of our data is that they consist of presences only and species absences can only rarely be inferred. To help adjust for this we used common widespread species as proxies for the four target species by using captures of these proxy species as indicators that the collecting methods used were appropriate to capture the target species, assuming then that large numbers of occurrences of the proxies with contemporaneous absence of the target species in the same samples supports inferences of probable absence of target species. We here report new and previously unpublished occurrences for these species and document westward range expansions for H. formosa and F. chrysotus, an eastward range expansion for P. formosa, and a pattern of possible range contraction and expansion for F. jenkinsi

Verification of Identifications of Cyprinid Specimens from the Colorado River Basin, Texas

Numerous published reports indicate that records of occurrence of Sharpnose Shiner, Notropis oxyrhynchus, in the Colorado River basin of Texas are the result of an introduction, though the species is clearly native in the adjacent Brazos River basin. We discovered previously mis-identified specimens of N. oxyrhynchus that extend the record of presence of the species in the Colorado basin much further back in time than previous authors realized, and conclude that the species was almost certainly native there. However, lack of the species in any of the many collections made in the basin over the last half century indicates a low probability that it still persists there.U.S. Fish & Wildlife Service, Arlington, Texas. FWS FBMS Agreement #: F12AP00622; CFDA Number/Title: 15.650; non-Profit, AcademicTexas Natural Science Cente

Lunar Surface Operations with Dual Rovers

Lunar Electric Rovers (LER) are currently being developed that are substantially more capable than the Apollo vehicle (LRN ,"). Unlike the LRV, the new LERs provide a pressurized cabin that serves as short-sleeve environment for the crew of two, including sleeping accommodations and other provisions that allow for long tern stays, possibly up to 60 days, on the hear surface, without the need to replenish consumables from some outside source, such as a lander or outpost. As a consequence, significantly larger regions may be explored in the future and traverse distances may be measured in a few hundred kilometers (1, 2). However, crew safety remains an overriding concern, and methods other than "walk back", the major operational constraint of all Apollo traverses, must be implemented to assure -at any time- the safe return of the crew to the lander or outpost. This then causes current Constellation plans to envision long-tern traverses to be conducted with 2 LERs exclusively, each carrying a crew of two: in case one rover fails, the other will rescue the stranded crew and return all 4 astronauts in a single LER to base camp. Recent Desert Research and Technology Studies (DRATS) analog field tests simulated a continuous 14 day traverse (3), covering some 135 km, and included a rescue operation that transferred the crew and diverse consumables from one LER to another these successful tests add substantial realism to the development of long-term, dual rover operations. The simultaneous utilization of 2 LERs is of course totally unlike Apollo and raises interesting issues regarding science productivity and mission operations, the thrust of this note

Field Test Performance of Junior Competitive Surf Athletes following a Core Strength Training Program

International Journal of Exercise Science 11(6): 696-707, 2018. Lower body and core muscular strength are essential for optimal performance in many sports and competitive surfers have similar strength demands when maneuvering a surfboard to achieve competition success. Presently, the use of unstable surfaces is excessively utilized by surf coaches and trainers and to date, research does not support this as an effective training method for long-term improvements. Therefore, the purpose of this study was to determine the effectiveness of an 8-week Core Strength Training Program (CSTP) on a battery of field tests specific to assessing core musculature and lower body strength for junior competitive surf athletes. Nineteen American junior competitive surf athletes (age:15.7±1.01yrs, height:1.77±0.007m, mass:64.67±9.08kg) completed pre- and post-tests with a transitional pre-season to in-season 8-week CSTP intervention. The battery of tests included: rotational power (RP), time to peak acceleration (TP), maximal acceleration (Ma), maximal countermovement jump (CMJ), estimated peak power (PP), core strength (CS), core endurance (CE), and rotational flexibility (RF). Means, standard deviations, RMANOVA with a significance level of p \u3c 0.05, and effect sizes were computed. Results demonstrated significant improvements in L.RP, TP, CMJ, PP, CS, and RF. Based on the results, the CSTP is an effective training program for surf coaches and strength and conditioning professionals to improve strength in the core musculature and lower body. In addition, we conclude implementation of the CSTP enhances athletic performance measurements which will likely increase competition success

Altered patterns of gene duplication and differential gene gain and loss in fungal pathogens

<p>Abstract</p> <p>Background</p> <p>Duplication, followed by fixation or random loss of novel genes, contributes to genome evolution. Particular outcomes of duplication events are possibly associated with pathogenic life histories in fungi. To date, differential gene gain and loss have not been studied at genomic scales in fungal pathogens, despite this phenomenon's known importance in virulence in bacteria and viruses.</p> <p>Results</p> <p>To determine if patterns of gene duplication differed between pathogens and non-pathogens, we identified gene families across nine euascomycete and two basidiomycete species. Gene family size distributions were fit to power laws to compare gene duplication trends in pathogens <it>versus </it>non-pathogens. Fungal phytopathogens showed globally altered patterns of gene duplication, as indicated by differences in gene family size distribution. We also identified sixteen examples of gene family expansion and five instances of gene family contraction in pathogenic lineages. Expanded gene families included those predicted to be important in melanin biosynthesis, host cell wall degradation and transport functions. Contracted families included those encoding genes involved in toxin production, genes with oxidoreductase activity, as well as subunits of the vacuolar ATPase complex. Surveys of the functional distribution of gene duplicates indicated that pathogens show enrichment for gene duplicates associated with receptor and hydrolase activities, while euascomycete pathogens appeared to have not only these differences, but also significantly more duplicates associated with regulatory and carbohydrate binding functions.</p> <p>Conclusion</p> <p>Differences in the overall levels of gene duplication in phytopathogenic species <it>versus </it>non-pathogenic relatives implicate gene inventory flux as an important virulence-associated process in fungi. We hypothesize that the observed patterns of gene duplicate enrichment, gene family expansion and contraction reflect adaptation within pathogenic life histories. These adaptations were likely shaped by ancient, as well as contemporary, intimate associations with monocot hosts.</p

A decade of monitoring Atlantic cod Gadus morhua spawning aggregations in Massachusetts Bay using passive acoustics

© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Caiger, P. E., Dean, M. J., DeAngelis, A. I., Hatch, L. T., Rice, A. N., Stanley, J. A., Tholke, C., Zemeckis, D. R., & Van Parijs, S. M. A decade of monitoring Atlantic cod Gadus morhua spawning aggregations in Massachusetts Bay using passive acoustics. Marine Ecology Progress Series, 635, (2020): 89-103, doi:10.3354/meps13219.Atlantic cod Gadus morhua populations in the northeast USA have failed to recover since major declines in the 1970s and 1990s. To rebuild these stocks, managers need reliable information on spawning dynamics in order to design and implement control measures; discovering cost-effective and non-invasive monitoring techniques is also favorable. Atlantic cod form dense, site-fidelic spawning aggregations during which they vocalize, permitting acoustic detection of their presence at such times. The objective of this study was to detect spawning activity of Atlantic cod using multiple fixed-station passive acoustic recorders to sample across Massachusetts Bay during the winter spawning period. A generalized linear modeling approach was used to investigate spatio-temporal trends of cod vocalizing over 10 consecutive winter spawning seasons (2007-2016), the longest such timeline of any passive acoustic monitoring of a fish species. The vocal activity of Atlantic cod was associated with diel, lunar, and seasonal cycles, with a higher probability of occurrence at night, during the full moon, and near the end of November. Following 2009 and 2010, there was a general decline in acoustic activity. Furthermore, the northwest corner of Stellwagen Bank was identified as an important spawning location. This project demonstrated the utility of passive acoustic monitoring in determining the presence of an acoustically active fish species, and provides valuable data for informing the management of this commercially, culturally, and ecologically important species.Thanks to Eli Bonnell, Genevieve Davis, Julianne Bonell, Samara Haver, and Eric Matzen for assistance in MARU deployments, Dana Gerlach and Heather Heenehan for help in passive acoustic data analysis, and the NEFSC passive acoustics group for useful discussions. Funding for 2007−2012 passive acoustic surveys was provided by Excelerate Energy and Neptune LNG to Cornell University. Fieldwork for 2013−2015 was funded through the 2013−2014 NOAA Saltonstall-Kennedy grant program (Award No. NA14NMF4270027), and jointly funded by The Nature Conservancy, Massachusetts Division of Marine Fisheries, and the Cabot Family Charitable Foundation. Funding for 2016 SoundTrap data was provided by NOAA’s Ocean Acoustics Program (4 Sanctuaries Project)

American Eel in Texas – what we do, don’t, and need to, know

Copy of the oral presentation made by the first author to the January 2016 meeting of the Texas Chapter of American Fisheries Society in Kerrville, Texas. Presentation is provided in several file formats. Data mapped in the presentation are also included in kml format (Google Earth).American Eel is undoubtedly one of the most studied freshwater fishes of North America. Many recent discoveries have added new insights that re-write important aspects of the “text book” knowledge of the species’ complex life history in ways that could have significant impacts on management. Despite all of this new information, debate about the species’ conservation status continues, and new threats, such as continued habitat loss and major clandestine fisheries driven by extremely high value in the global market, have further complicated management. Though USFWS recently decided that the species does not merit listing as “Endangered,” in 2012 Canada changed that country’s assessment of the species’ status from “Special Concern” (since 2006) to “Threatened” and IUCN upped its classification in 2013 to “Endangered.” Ontario has considered it “Endangered” since 2007. All U.S. Atlantic states vowed to work together to produce, in 1999, the American Eel Benchmark Stock Assessment, which mandated each state conduct standardized monitoring of recruitment and later, mandatory catch and effort monitoring. Given all that activity and data generation, it is remarkable that still so little is known about the populations of the Gulf of Mexico (GOM) and its tributary rivers that making any management decisions in that large, neglected part of the species’ range is virtually impossible. The Fishes of Texas Project team has been collating and improving the limited and scattered data on occurrences of the species in the region and concludes it important to promote a broad scale (Gulf of Mexico) collaborative community effort to acquire and share data and carefully curated specimens and, hopefully, develop a GOM-wide collaborative research and management plan like that implemented by Atlantic states. Here we’ll review the literature and state of knowledge about the species in Texas and GOM, and suggest ways to begin work toward such an effort.University of Texas at AustinIntegrative Biolog

HOXA13 Is Essential for Placental Vascular Patterning and Labyrinth Endothelial Specification

In eutherian mammals, embryonic growth and survival is dependent on the formation of the placenta, an organ that facilitates the efficient exchange of oxygen, nutrients, and metabolic waste between the maternal and fetal blood supplies. Key to the placenta's function is the formation of its vascular labyrinth, a series of finely branched vessels whose molecular ontogeny remains largely undefined. In this report, we demonstrate that HOXA13 plays an essential role in labyrinth vessel formation. In the absence of HOXA13 function, placental endothelial cell morphology is altered, causing a loss in vessel wall integrity, edema of the embryonic blood vessels, and mid-gestational lethality. Microarray analysis of wild-type and mutant placentas revealed significant changes in endothelial gene expression profiles. Notably, pro-vascular genes, including Tie2 and Foxf1, exhibited reduced expression in the mutant endothelia, which also exhibited elevated expression of genes normally expressed in lymphatic or sinusoidal endothelia. ChIP analysis of HOXA13–DNA complexes in the placenta confirmed that HOXA13 binds the Tie2 and Foxf1 promoters in vivo. In vitro, HOXA13 binds sequences present in the Tie2 and Foxf1 promoters with high affinity (Kd = 27–42 nM) and HOXA13 can use these bound promoter regions to direct gene expression. Taken together, these findings demonstrate that HOXA13 directly regulates Tie2 and Foxf1 in the placental labyrinth endothelia, providing a functional explanation for the mid-gestational lethality exhibited by Hoxa13 mutant embryos as well as a novel transcriptional program necessary for the specification of the labyrinth vascular endothelia