Design and test of the Digital Opto Hybrid Module for the CMS Tracker Inner Barrel and Disks.

One of the most important tasks to be performed in the CMS Tracker detector is the communication between hundreds of silicon modules and the central Control System under the supervision of the Data Acquisition System. To manage such complexity, modules are grouped in a hierarchical structure. Each group is controlled by a Communication and Control Unit (CCU). Several CCU form a ring with a Front End Controller as master. The entire Tracker Inner Barrel and Disks detector contains roughly 100 such rings called Control Rings. A description is given here of the implemented ring architecture for the detector and of the solutions found to provide a reliable and easy way to interconnect these groups

The Resolved Narrow Line Region in NGC4151

We present slitless spectra of the Narrow Line Region (NLR) in NGC4151 from the Space Telescope Imaging Spectrograph (STIS) on HST, and investigate the kinematics and physical conditions of the emission line clouds in this region. Using medium resolution (~0.5 Angstrom) slitless spectra at two roll angles and narrow band undispersed images, we have mapped the NLR velocity field from 1.2 kpc to within 13 pc (H_o=75 km/s/Mpc) of the nucleus. The inner biconical cloud distribution exhibits recessional velocities relative to the nucleus to the NE and approaching velocities to the SW of the nucleus. We find evidence for at least two kinematic components in the NLR. One kinematic component is characterized by Low Velocities and Low Velocity Dispersions (LVLVD clouds: |v| < 400 km/s, and Delta_v < 130 km/s). This population extends through the NLR and their observed kinematics may be gravitationally associated with the host galaxy. Another component is characterized by High Velocities and High Velocity Dispersions (HVHVD clouds: 400 130 km/s). This set of clouds is located within 1.1 arcsec (~70pc) of the nucleus and has radial velocities which are too high to be gravitational in origin, but show no strong correlation between velocity or velocity dispersion and the position of the radio knots. Outflow scenarios will be discussed as the driving mechanism for these HVHVD clouds.Comment: 38 pages, 14 figures, accepted by ApJ. For higher resolution images see http://www.pha.jhu.edu/~kaiser

Spectrophotometry of HII Regions, Diffuse Ionized Gas and Supernova Remnants in M31: The Transition from Photo- to Shock-Ionization

We present results of KPNO 4-m optical spectroscopy of discrete emission-line nebulae and regions of diffuse ionized gas (DIG) in M31. Long-slit spectra of 16 positions in the NE half of M31 were obtained over a 5-15 kpc range in radial distance from the center of the galaxy. The spectra have been used to confirm 16 supernova remnant candidates from the Braun & Walterbos (1993) catalog. The slits also covered 46 HII regions which show significant differences among the various morphological types (center-brightened, diffuse, rings). Radial gradients in emission-line ratios such as [OIII]/H$\beta$ and [OII]/[OIII] are observed most prominently in the center-brightened HII regions. These line ratio trends are either much weaker or completely absent in the diffuse and ring nebulae. The line ratio gradients previously seen in M31 SNRs (Blair, Kirshner, & Chevalier 1981; 1982) are well reproduced by our new data. The spectra of center-brightened HII regions and SNRs confirm previous determinations of the radial abundance gradient in M31. We use diagnostic diagrams which separate photoionized gas from shock-ionized gas to compare the spectral properties of HII regions, SNRs and DIG. This analysis strengthens earlier claims (Greenawalt, Walterbos, & Braun 1997) that the DIG in the disk of M31 is photoionized by a dilute radiation field.Comment: 45 pages, 9 figures, 7 tables, to appear in the Astronomical Journal (December 1999

Discovery of a nuclear gas bar feeding the active nucleus in Circinus

We report the discovery of gas inflow motions towards the active nucleus of the Circinus galaxy caused by the non-axisymmetric potential of a nuclear gas bar. Evidence for dust associated with the bar comes from the HST/NICMOS H-K color map, whereas the streaming motions along the gas bar are seen in the velocity field of the H2 S(1)(1-0) emission line. The gas bar is about 100 pc long with a visual extinction in excess of 10 mag. Indication for the gaseous nature of this bar comes from the lack of a stellar counterpart even in the K band where the extinction is greatly reduced. We also use the NICMOS emission line images (Pa-alpha, [SiVI], and [FeII]) to study the innermost region of the ionization cones and the nuclear star forming activity. We discuss the possible relationship of these components with the gaseous bar.Comment: 14 pages, 7 figures (3 color plates), accepted for publication in Ap

The He II Emitting Nebula N44C in the LMC: Optical/UV Spectroscopy of the Nebula and its Ionizing Star

We present HST spectroscopy and imaging, along with new ground-based spectroscopy and ROSAT HRI imaging, of the He II emitting nebula N44C and its ionizing star. A GHRS spectrogram of the ionizing star yields a spectral type of about O7 for the star. The lack of P Cygni profiles for Si IV and C IV indicates that the star is not a supergiant. The nebular abundances in the ionized gas are consistent with average abundances for LMC H II regions, with the possible exception that nitrogen may be enhanced. Enrichment by a former evolved companion star is not evident. A long-slit echelle spectrogram in H-alpha + [N II] shows no evidence for high-velocity gas in N44C. This rules out high-velocity shocks as the source of the nebular He II emission. A 108 ks ROSAT HRI image of N44C shows no X-ray point source to a 3-sigma upper limit L(X) < 10^34 erg s^-1 in the 0.1-2.0 keV band. Based on new measurements of the electron density in the He II emitting region, we derive recombination timescales of approximately 20 yrs for He^+2 and approximately 4 yrs for Ne^+4. If N44C is a fossil X-ray ionized nebula, this places severe constraints on when the putative X-ray source could have turned off. The presence of strong [Ne IV] emission in the nebula is puzzling if the ionizing source has turned off. It is possible the system is related to the Be X-ray binaries, although the O star in N44C does not show Be characteristics at the present time. Monitoring of X-rays and He II emission from the nebula, as well as a radial velocity study of the ionizing star, are needed to fully understand the emission line spectrum of N44C.Comment: 37 pages, 7 figures (1 color .gif image); accepted for publication in the 10 Dec 2000 Astrophysical Journal. Complete PostScript and PDF versions can also be obtained at http://ocotillo.as.arizona.edu/~dgarnet

Roles of Major Facilitator Superfamily Transporters in Phosphate Response in Drosophila

The major facilitator superfamily (MFS) transporter Pho84 and the type III transporter Pho89 are responsible for metabolic effects of inorganic phosphate in yeast. While the Pho89 ortholog Pit1 was also shown to be involved in phosphate-activated MAPK in mammalian cells, it is currently unknown, whether orthologs of Pho84 have a role in phosphate-sensing in metazoan species. We show here that the activation of MAPK by phosphate observed in mammals is conserved in Drosophila cells, and used this assay to characterize the roles of putative phosphate transporters. Surprisingly, while we found that RNAi-mediated knockdown of the fly Pho89 ortholog dPit had little effect on the activation of MAPK in Drosophila S2R+ cells by phosphate, two Pho84/SLC17A1–9 MFS orthologs (MFS10 and MFS13) specifically inhibited this response. Further, using a Xenopus oocyte assay, we show that MSF13 mediates uptake of [³³P]-orthophosphate in a sodium-dependent fashion. Consistent with a role in phosphate physiology, MSF13 is expressed highest in the Drosophila crop, midgut, Malpighian tubule, and hindgut. Altogether, our findings provide the first evidence that Pho84 orthologs mediate cellular effects of phosphate in metazoan cells. Finally, while phosphate is essential for Drosophila larval development, loss of MFS13 activity is compatible with viability indicating redundancy at the levels of the transporters.National Institutes of Health (U.S.) (NIDDK 5K08DK078361)Harvard Catalys

Genetic Determinants of Phosphate Response in Drosophila

Phosphate is required for many important cellular processes and having too little phosphate or too much can cause disease and reduce life span in humans. However, the mechanisms underlying homeostatic control of extracellular phosphate levels and cellular effects of phosphate are poorly understood. Here, we establish Drosophila melanogaster as a model system for the study of phosphate effects. We found that Drosophila larval development depends on the availability of phosphate in the medium. Conversely, life span is reduced when adult flies are cultured on high phosphate medium or when hemolymph phosphate is increased in flies with impaired Malpighian tubules. In addition, RNAi-mediated inhibition of MAPK-signaling by knockdown of Ras85D, phl/D-Raf or Dsor1/MEK affects larval development, adult life span and hemolymph phosphate, suggesting that some in vivo effects involve activation of this signaling pathway by phosphate. To identify novel genetic determinants of phosphate responses, we used Drosophila hemocyte-like cultured cells (S2R+) to perform a genome-wide RNAi screen using MAPK activation as the readout. We identified a number of candidate genes potentially important for the cellular response to phosphate. Evaluation of 51 genes in live flies revealed some that affect larval development, adult life span and hemolymph phosphate levels

S -Adenosyl- l -homocysteine in brain

Administration of methionine sulfoximine (MSO) to rats and mice significantly decreased cerebral levels of S -adenosyl- l -homocysteine (AdoHcy). Concurrent administration of methionine prevented this decrease and, when methionine was given alone, significantly elevated AdoHcy levels resulted in both species. Regionally, AdoHcy levels varied from 20 nmol/g in rat cerebellum and spinal cord to about 60 nmol/g in hypothalamus and midbrain. MSO decreased AdoHcy in all regions tested except striatum, midbrain, and spinal cord. AdoMet/AdoHcy ratios (methylation index) varied from 0.48 in hypothalamus to 2.4 in cerebellum, and MSO administration decreased these ratios in all regions except hypothalamus. AdoHcy hydrolase activity was lowest in hypothalamus, highest in brainstem and, generally, varied inversely with regional AdoHcy levels. MSO decreased AdoHcy hydrolase activity in all regions except hypothalamus and spinal cord. Cycloleucine administration resulted in significantly decreased levels of mouse brain AdoHcy, whereas the administration of dihydroxyphenylalanine (DOPA) failed to affect AdoHcy levels. It is concluded that (a) cerebral AdoHcy levels are more tightly regulated than are those of AdoMet after MSO administration, (b) slight fluctuations of AdoHcy levels may be important in regulating AdoHcy hydrolase activity and hence AdoHcy catabolism in vivo, (c) the AdoMet/AdoHcy ratio reflects the absolute AdoMet concentration rather than the transmethylation flux, (d) the decreased AdoMet levels in midbrain, cortex, and striatum after MSO with no corresponding decrease in AdoHcy suggest an enhanced AdoMet utilization, hence an increased transmethylation in the MSO preconvulsant state.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/45411/1/11064_2004_Article_BF00966019.pd