Age and sex-associated variation in the multi-site microbiome of an entire social group of free-ranging rhesus macaques

Background: An individual’s microbiome changes over the course of its lifetime, especially during infancy, and again in old age. Confounding factors such as diet and healthcare make it difficult to disentangle the interactions between age, health, and microbial changes in humans. Animal models present an excellent opportunity to study age- and sex-linked variation in the microbiome, but captivity is known to influence animal microbial abundance and composition, while studies of free-ranging animals are typically limited to studies of the fecal microbiome using samples collected non-invasively. Here, we analyze a large dataset of oral, rectal, and genital swabs collected from 105 free-ranging rhesus macaques (Macaca mulatta, aged 1 month-26 years), comprising one entire social group, from the island of Cayo Santiago, Puerto Rico. We sequenced 16S V4 rRNA amplicons for all samples. Results: Infant gut microbial communities had significantly higher relative abundances of Bifidobacterium and Bacteroides and lower abundances of Ruminococcus, Fibrobacter, and Treponema compared to older age groups, consistent with a diet high in milk rather than solid foods. The genital microbiome varied widely between males and females in beta-diversity, taxonomic composition, and predicted functional profiles. Interestingly, only penile, but not vaginal, microbiomes exhibited distinct age-related changes in microbial beta-diversity, taxonomic composition, and predicted functions. Oral microbiome composition was associated with age, and was most distinctive between infants and other age classes. Conclusions: Across all three body regions, with notable exceptions in the penile microbiome, while infants were distinctly different from other age groups, microbiomes of adults were relatively invariant, even in advanced age. While vaginal microbiomes were exceptionally stable, penile microbiomes were quite variable, especially at the onset of reproductive age. Relative invariance among adults, including elderly individuals, is contrary to findings in humans and mice. We discuss potential explanations for this observation, including that age-related microbiome variation seen in humans may be related to changes in diet and lifestyle. 4_dARqKdohA9mAZyu7q9YNVideo abstrac

Understanding the Correlates Between Care-Recipient Age and Caregiver Burden, Work-Family Conflict, Job Satisfaction, and Turnover Intentions

Although the caregiver literature has explored a wide array of different variables, there is a gap in research on how demographics of the care recipient affect their caregiver. Using data from a diverse sample of 1007 unpaid caregivers, individuals were separated into four groups based on care recipient age; childcare, adultcare, eldercare, and those with care recipients from multiple age categories. Then, following previous literature, childcare was split into four groups based on the age of the youngest child cared for (0–2 years; 3–5 years; 6–12 years; and 13–17 years). Group differences were found in work-family conflict (time) and five types of caregiver burden. Specifically, time-dependence burden differed most between groups with caregivers of children (0–2 years) having the highest levels and caregivers of children (13–17) having the lowest levels. There were no differences found between groups for job satisfaction, turnover intentions, or family-work conflict. Additional differences were found when considering caregivers’ gender. This research is an exploratory step in understanding how age of care recipient relates to different outcomes for caregivers. Implications are discussed