Ovarian responses and conception rates in response to gNrh, Hcg, and progesterone

Dairy Research, 2006 is known as Dairy Day, 2006We hypothesized that increasing concentrations of progesterone after artificial insemination (AI) would increase fertility. Our objective was to assess changes in ovarian structures, incidence of ovulation, and change in serum progesterone in response to GnRH, human chorionic gonadotropin (hCG), or exogenous progesterone (controlled internal drug release; CIDR insert) treatment, beginning 4 to 9 days after AI (d 0) and again 7 days later (Exp. 1). Blood was collected from 753 cows in 3 herds on days 0 and 7. Ovaries of 162 cows in 1 herd were scanned and mapped to confirm the presence a corpus luteum (CL), and cows were assigned randomly to serve as control (n = 41) or to receive a CIDR insert for 7 days (n = 41), 100 μg of GnRH (n = 40), or 3,300 IU of hCG (n = 40). More cows were induced to ovulate in response to GnRH (60%) and hCG (78%), compared with control (2.4%). Compared with control, cows treated with GnRH or hCG had more induced CL (d 7) and more total CL (d 7), but serum progesterone was increased only in response to hCG. Volume of the original luteal structures was increased by hCG, but tended to be reduced by CIDR and GnRH, compared with luteal volume in control. Total CL volume was increased by hCG, but reduced by CIDR, compared with CL volume of control. In Exp. 2, cows in 5 herds were used to assess conception rates in response to the same treatments described in Exp. 1: control (n = 708), CIDR (n = 711), GnRH (n = 719), and hCG (n = 714). Tendencies for interactions of treatment × herd and treatment × lactation group were detected, but no 3-way interactions were found. Treatment with hCG increased conception rates in second-lactation cows. The CIDR tended to increase, and hCG increased, conception rates in 2 herds, whereas the CIDR decreased conception rates in 1 herd. We concluded that GnRH and hCG effectively induced ovulation, and increased number of CL, but only hCG increased serum progesterone. Further, treatment with the CIDR or hCG increased conception rates, but only in some herds

Effect of Simultaneous Thawing of Multiple 0.5-mL Straws of Semen and Sequence of Insemination on Conception Rate in Dairy Cattle

The objectives of this study were to determine 1) the effect of simultaneous thawing of multiple 0.5-mL straws of semen and sequence of insemination (first, second, third, or fourth) on conception rates in dairy cattle, 2) whether the conception rates achieved following AI by professional AI (PAI) technicians and herdsman-inseminators (HI) differed, and 3) the effect of elapsed time from initiation of thawing straws of semen to seminal deposition on conception rates in dairy cattle. Four dairies with PAI and four with HI participated in the study. Initial data recorded included beginning thaw time, cow identification number, and time of seminal deposition. Herd records were retrieved following pregnancy diagnosis. Conception rates of dairy cows (n=1025) were not affected by sequence of insemination (first, second, third, or fourth). Conception rates for herds using PAI were 40, 47, 41, and 50%. Conception rates for herds using HI were 24, 20, 33, and 30%. Average conception rates of dairy cows differed between PAI and HI (45 vs. 27%, respectively). The difference in mean conception rate achieved by PAI and HI was not attributable to milk production, parity, service number or stage of lactation. The elapsed time from initial thaw to completion of fourth AI was shorter for PAI than for HI, 7.6 vs. 10.9min, respectively. Although the average conception rate differed between PAI and HI, elapsed time from initial thaw to completion of fourth AI and sequence of insemination (first, second, third, or fourth) had no effect on conception rate within inseminator group

Estrus synchronization of replacement beef heifers by using GnRH, prostaglandin F2α (PGF), and progesterone (CIDR): a multi-location study

Our objectives were to determine whether a fixed-timed artificial insemination (TAI) protocol could yield similar fertility rates to a protocol requiring detection of estrus and whether an injection of gonadotropin hormone-releasing hormone (GnRH) at CIDR (vaginal insert containing progesterone) insertion enhances pregnancy rates. Replacement beef heifers (n=2,077) from 12 locations were assigned randomly to each of four estrussynchronization protocols. All heifers received a CIDR for 7 days, and an injection of prostaglandin F2α (PGF) on the day of CIDR removal. For treatment EAI, heifers were observed for estrus for 84 hours after PGF administration and were inseminated 6 to 12 hours after observed estrus. Any heifer not detected in estrus was injected with GnRH, followed by TAI. For treatment GnRH+EAI, heifers were treated as those for EAI, but also received GnRH at the time of CIDR insertion. For treatment TAI, heifers received a single TAI at 60 hours after PGF administration. For treatment GnRH+TAI, heifers were treated as those for TAI, but also received GnRH at CIDR insertion. The percentage of heifers cycling at the initiation of estrus-synchronization was 91%; the percentage of cycling heifers among locations ranged from 78 to 100%. Overall pregnancy rates among locations ranged from 38 to 74%. Pregnancy rates were 57.3, 54.5, 53.1, and 49.1% for GnRH+EAI, EAI, GnRH+TAI, and TAI, respectively. Although no statistically significant differences in pregnancy rates among treatments were observed, the GnRH+EAI treatment achieved the numerically greatest pregnancy rates. In addition, the GnRH+TAI protocol provides an alternative that allows producers to synchronize heifers without detection of estrus

Estrus synchronization of suckled beef cows by using GnRH, prostaglandin F2α (PGF), and progesterone (CIDR): a multi-location study

Our objectives were to determine whether a fixed-time artificial insemination (TAI) protocol could yield pregnancy rates similar to a protocol requiring detection of estrus and whether inclusion of a CIDR (a vaginal insert containing progesterone) in protocols using gonadotropin-releasing hormone (GnRH) and prostaglandin F2α (PGF) would enhance fertility. Postpartum suckled beef cows (n = 2,630) from 14 locations were assigned randomly to each of five estrus-synchronization protocols using PGF with GnRH and(or) a CIDR. Protocols were Control, CO-Synch, COSynch+ CIDR, Hybrid-Synch, and Hybrid-Synch+CIDR. The percentage of cows cycling at the initiation of estrus synchronization was 66.8%, the percentage of cycling cows ranging from 38 to 90% among locations. Overall pregnancy among locations ranged from 39% to 67%. Pregnancy rates were greatest for the Hybrid-Synch+CIDR (57.9%) treatment, although not significantly different from the CO-Synch+CIDR (53.6%) and Hybrid-Synch (53.0%) treatments, but greater than the Control (52.3%) and CO-Synch (43.4%), which yielded the poorest pregnancy rates. Overall, the Hybrid-Synch+CIDR protocol (AI after detected estrus for 3 days, and then a clean-up TAI) achieved the greatest pregnancy rates, but CO-Synch+CIDR is a reliable, fixed-time AI protocol that gives producers the option to eliminat