2,600 research outputs found
γ-H2AX foci as in vivo effect biomarker in children emphasize the importance to minimize x-ray doses in paediatric CT imaging
Objectives: Investigation of DNA damage induced by CT x-rays in paediatric patients versus patient dose in a multicentre setting.
Methods: From 51 paediatric patients (median age, 3.8 years) who underwent an abdomen or chest CT examination in one of the five participating radiology departments, blood samples were taken before and shortly after the examination. DNA damage was estimated by scoring gamma-H2AX foci in peripheral blood T lymphocytes. Patient-specific organ and tissue doses were calculated with a validated Monte Carlo program. Individual lifetime attributable risks (LAR) for cancer incidence and mortality were estimated according to the BEIR VII risk models.
Results: Despite the low CT doses, a median increase of 0.13 gamma-H2AX foci/cell was observed. Plotting the induced gamma-H2AX foci versus blood dose indicated a low-dose hypersensitivity, supported also by an in vitro dose-response study. Differences in dose levels between radiology centres were reflected in differences in DNA damage. LAR of cancer mortality for the paediatric chest CT and abdomen CT cohort was 0.08 and 0.13% respectively.
Conclusion: CT x-rays induce DNA damage in paediatric patients even at low doses and the level of DNA damage is reduced by application of more effective CT dose reduction techniques and paediatric protocols
The Abbey of the Dunes (Koksijde) and Noordduinen: the environmental link restored
The Abbey of the Dunes (Ten Duinen) at Koksijde, founded in the 12th century and abandoned around 1600, forms the subject of a land use planning project realized by the Flemish Land Agency (Vlaamse Landmaatschappij). Initially the project focussed on the museum and the integration of the abbey ruins in an open-air museum. However the site presented an opportunity to involve the Noordduinen, bordering on the abbey park, in a nature restoration project. The key intention was to restore the landscape connection between the abbey and the dunes, as it was in the Middle Ages. The proposed actions consist of breaking up a road, deviation of the traffic, upgrading the abbey park and start of a nature restoration project in the Noordduinen. The main bottlenecks were deviation of the traffic, excavation and preservation of archaeological finds and intensive recreational use of the Noordduinen. Continuous consultation and deliberation with all involved authorities and with the local inhabitants of Koksijde resulted in a plan supported by the majority of the community
Inference of the genetic network regulating lateral root initiation in Arabidopsis thaliana
Regulation of gene expression is crucial for organism growth, and it is one of the challenges in Systems Biology to reconstruct the underlying regulatory biological networks from transcriptomic data. The formation of lateral roots in Arabidopsis thaliana is stimulated by a cascade of regulators of which only the interactions of its initial elements have been identified. Using simulated gene expression data with known network topology, we compare the performance of inference algorithms, based on different approaches, for which ready-to-use software is available. We show that their performance improves with the network size and the inclusion of mutants. We then analyse two sets of genes, whose activity is likely to be relevant to lateral root initiation in Arabidopsis, by integrating sequence analysis with the intersection of the results of the best performing methods on time series and mutants to infer their regulatory network. The methods applied capture known interactions between genes that are candidate regulators at early stages of development. The network inferred from genes significantly expressed during lateral root formation exhibits distinct scale-free, small world and hierarchical properties and the nodes with a high out-degree may warrant further investigation
WZW-like Action for Heterotic String Field Theory
We complete the construction of the Neveu-Schwarz sector of heterotic string
field theory begun in hep-th/0406212 by giving a closed-form expression for the
action and gauge transformations. Just as the Wess-Zumino-Witten (WZW) action
for open superstring field theory can be constructed from pure-gauge fields in
bosonic open string field theory, our heterotic string field theory action is
constructed from pure-gauge fields in bosonic closed string field theory. The
construction involves a simple alternative form of the WZW action which is
consistent with the algebraic structures of closed string field theory.Comment: 22 pages, no figures, LaTeX2
San Francisco Bay Area corporate history : a selected annotated bibliography
Human activities, among which dredging and land use change in river basins,
are altering estuarine ecosystems. These activities may result in changes in
sedimentary processes, affecting biodiversity of sediment macrofauna. As
macrofauna controls sediment chemistry and fluxes of energy and matter between
water column and sediment, changes in the structure of macrobenthic
communities could affect the functioning of an entire ecosystem. We assessed
the impact of sediment deposition on intertidal macrobenthic communities and
on rates of an important ecosystem function, i.e. sediment community oxygen
consumption (SCOC). An experiment was performed with undisturbed sediment
samples from the Scheldt river estuary (SW Netherlands). The samples were
subjected to four sedimentation regimes: one control and three with a
deposited sediment layer of 1, 2 or 5 cm. Oxygen consumption was measured
during incubation at ambient temperature. Luminophores applied at the
surface, and a seawater–bromide mixture, served as tracers for bioturbation
and bio-irrigation, respectively. After incubation, the macrofauna was
extracted, identified, and counted and then classified into functional groups
based on motility and sediment reworking capacity. Total macrofaunal
densities dropped already under the thinnest deposits. The most affected
fauna were surficial and low-motility animals, occurring at high densities in
the control. Their mortality resulted in a drop in SCOC, which decreased
steadily with increasing deposit thickness, while bio-irrigation and
bioturbation activity showed increases in the lower sediment deposition
regimes but decreases in the more extreme treatments. The initial increased
activity likely counteracted the effects of the drop in low-motility, surficial
fauna densities, resulting in a steady rather than sudden fall in oxygen
consumption. We conclude that the functional identity in terms of motility
and sediment reworking can be crucial in our understanding of the regulation
of ecosystem functioning and the impact of habitat alterations such as
sediment deposition
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Calcium puffs are generic InsP<sub>3</sub>-activated elementary calcium signals and are downregulated by prolonged hormonal stimulation to inhibit cellular calcium responses
Elementary Ca2+ signals, such as "Ca2+ puffs", which arise from the activation of inositol 1,4,5-trisphosphate receptors, are building blocks for local and global Ca2+ signalling. We characterized Ca2+ puffs in six cell types that expressed differing ratios of the three inositol 1,4,5-trisphosphate receptor isoforms. The amplitudes, spatial spreads and kinetics of the events were similar in each of the cell types. The resemblance of Ca2+ puffs in these cell types suggests that they are a generic elementary Ca2+ signal and, furthermore, that the different inositol 1,4,5-trisphosphate isoforms are functionally redundant at the level of subcellular Ca2+ signalling. Hormonal stimulation of SH-SY5Y neuroblastoma cells and HeLa cells for several hours downregulated inositol 1,4,5-trisphosphate expression and concomitantly altered the properties of the Ca2+ puffs. The amplitude and duration of Ca2+ puffs were substantially reduced. In addition, the number of Ca2+ puff sites active during the onset of a Ca2+ wave declined. The consequence of the changes in Ca2+ puff properties was that cells displayed a lower propensity to trigger regenerative Ca2+ waves. Therefore, Ca2+ puffs underlie inositol 1,4,5-trisphosphate signalling in diverse cell types and are focal points for regulation of cellular responses
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