Protective role of carbonic anhydrases III and VII in cellular defense mechanisms upon redox unbalance

Under oxidative stress conditions, several constitutive cellular defense systems are activated, which involve both enzymatic systems and molecules with antioxidant properties such as glutathione and vitamins. In addition, proteins containing reactive sulfhydryl groups may eventually undergo reversible redox modifications whose products act as protective shields able to avoid further permanent molecular oxidative damage either in stressful conditions or under pathological circumstances. After the recovery of normal redox conditions, the reduced state of protein sulfhydryl groups is restored. In this context, carbonic anhydrases (CAs) III and VII, which are human metalloenzymes catalyzing the reversible hydration of carbon dioxide to bicarbonate and proton, have been identified to play an antioxidant role in cells where oxidative damage occurs. Both proteins are mainly localized in tissues characterized by a high rate of oxygen consumption, and contain on their molecular surface two reactive cysteine residues eventually undergoing S-glutathionylation. Here, we will provide an overview on the molecular and functional features of these proteins highlighting their implications into molecular processes occurring during oxidative stress conditions

Insights into the role of reactive sulfhydryl groups of Carbonic Anhydrase III and VII during oxidative damage

Carbonic anhydrases (CAs) III and VII are two cytosolic isoforms of the α-CA family which catalyze the physiological reaction of carbon dioxide hydration to bicarbonate and proton. Despite these two enzymes share a 49% sequence identity and present a very similar three-dimensional structure, they show profound differences when comparing the specific activity for CO2 hydration reaction, with CA VII being much more active than CA III. Recently, CA III and CA VII have been proposed to play a new role as scavenger enzymes in cells where oxidative damage occurs. Here, we will examine functional and structural features of these two isoforms giving insights into their newly proposed protective role against oxidative stress

Wireless resonant energy link for pulse generators implanted in the chest

This study investigates the feasibility of using the MedRadio band for wirelessly transmitting power to a rechargeable medical device implanted in the chest. In particular, a wireless energy link operating at 403 MHz and based on an inductive coupling between two planar resonators is considered, and its performance is assessed through simulations and experimental tests. Results show that a maximum value of the measured power transfer efficiency of ~23% is achieved. Furthermore, the possibility of using the link for recharging a lithium-ion battery of a pulse generator implanted in the chest at a depth of 5 mm is discussed, and the compliance with safety regulations is verified. Reported results demonstrate that the proposed link can be used to charge a lithium-ion battery with a capacity up to 7.2 mAh, while inducing a peak of the specific absorption rate averaged over 1 g of tissue < 1.6 W/kg

Endoscopic Resection of Esophageal Lymphangioma Incidentally Discovered

A pedunculated lymphangioma of the esophagus was unexpectedly discovered during an endoscopic investigation performed for epigastric pain in a patient affected by diabetic arteriopathy treated with antiplatelet drugs. The patient neither complained of dysphagia nor other symptoms related to the presence of the lymphangioma which therefore can be considered as an endoscopic “incidentaloma”

Functional assessment of cancer therapy questionnaire for breast cancer (FACT-B+4): Italian version validation

BACKGROUND: Improvements in breast cancer diagnosis and treatment led to an increased incidence of survivors' rate. The healthcare system has to face new problems related not only to the treatment of the disease, but also to the management of the quality of life after the diagnosis. The aim of this study was to validate the Italian version of the Functional Assessment of Cancer Therapy - Breast (FACT-B+4) questionnaire and to evaluate its reliability. METHODS: The questionnaire was administered twice, with an interval of three days between each administration, to a cohort of women of the Breast Surgical Unit, PoliclincoUmberto I. Cronbach's alpha was used as a measure of the internal consistency of the Italian version. RESULTS: The Italian version of the tool was administered to 55 subjects. The Cronbach's alpha for most scores registered values >0.7, both at baseline and at the follow-up analysis, therefore the subscale showed good internal consistency. CONCLUSIONS: The Italian version of FACT-B+4 demonstrated acceptable reliability properties in the Breast Unit patients. The use of this questionnaire seemed to be effective and in line with the results derived from the English and Spanishversions. Internal consistency and validity had similar performance results

Eddy diffusivity derived from drifter data for dispersion model applications

Ocean transport and dispersion processes are at the present time simulated using Lagrangian stochastic models coupled with Eulerian circulation models that are supplying analyses and forecasts of the ocean currents at unprecedented time and space resolution. Using the Lagrangian approach, each particle displacement is described by an average motion and a fluctuating part. The first one represents the advection associated with the Eulerian current field of the circulation models while the second one describes the sub-grid scale diffusion. The focus of this study is to quantify the sub-grid scale diffusion of the Lagrangian models written in terms of a horizontal eddy diffusivity. Using a large database of drifters released in different regions of the Mediterranean Sea, the Lagrangian sub-grid scale diffusion has been computed, by considering different regimes when averaging statistical quantities. In addition, the real drifters have been simulated using a trajectory model forced by OGCM currents, focusing on how the Lagrangian properties are reproduced by the simulated trajectories

Performance evaluation of an ORC unit integrated to a waste heat recovery system in a steel mill

Waste heat revalorization creates interesting opportunities to energy intensive industries. In the present project, a large-scale ORC pilot plant along with a waste heat recovery unit (WHRU) in a steel mill has been designed, commissioned and operated. The plant is part of the European Commission funded PITAGORAS project and it has been installed at ORI MARTIN in Brescia (Italy). Waste heat is recovered from the fumes of the Electric Arc Furnace (EAF) to produce saturated steam which is then delivered to a district heating (DH) network during heating season and to the ORC for electricity generation during the rest of the year. The main challenge was the integration of these systems in a single plant since the heat source is highly unstable and steady heat load is preferable for the DH and ORC for their safe operation. A steam accumulator of 150m3 volume was implemented between the WHRU and the ORC/DH systems to maintain a steady discharge pressure, to reduce the fast transients and to extend the supply over longer periods. The ORC has a nominal power output of 1,8MW and the preliminary results of the first weeks of operation of the ORC unit resulted in a net efficiency of 21.7%. Currently the plant is undergoing monitoring campaign which will provide additional data to further evaluate and optimize the system.The research leading to these results has received funding from the European Union Seventh Framework Programme FP7/2007-2013 under grant agreement n° ENER / FP7EN / 314596 / PITAGORAS

Interaction data are identifiable even across long periods of time

Fine-grained records of people’s interactions, both offline and online, are collected at large scale. These data contain sensitive information about whom we meet, talk to, and when. We demonstrate here how people’s interaction behavior is stable over long periods of time and can be used to identify individuals in anonymous datasets. Our attack learns the profile of an individual using geometric deep learning and triplet loss optimization. In a mobile phone metadata dataset of more than 40k people, it correctly identifies 52% of individuals based on their 2-hop interaction graph. We further show that the profiles learned by our method are stable over time and that 24% of people are still identifiable after 20 weeks. Our results suggest that people with well-balanced interaction graphs are more identifiable. Applying our attack to Bluetooth close-proximity networks, we show that even 1-hop interaction graphs are enough to identify people more than 26% of the time. Our results provide strong evidence that disconnected and even re-pseudonymized interaction data can be linked together making them personal data under the European Union’s General Data Protection Regulation

Direct interactions among Ret, GDNF and GFRalpha1 molecules reveal new insights into the assembly of a functional three-protein complex.

The glial-cell-line-derived neurotrophic factor (GDNF) ligand activates the Ret receptor through the assembly of a multiprotein complex, including the GDNF family receptor α1 (GFRα1) molecule. Given the neuroprotective role of GDNF, there is an obvious need to precisely identify the structural regions engaged in direct interactions between the three molecules. Here, we combined a functional approach for Ret activity (in PC12 cells) to cross-linking experiments followed by MS-MALDI to study the interactions among the purified extracellular region of the human Ret, GDNF and GFRα1 molecules. This procedure allowed us to identify distinct regions of Ret that are physically engaged in the interaction with GDNF and GFRα1. The lack of these regions in a recombinant Ret form results in the failure of both structural and functional binding of Ret to GFRα1/GDNF complex. Furthermore, a model for the assembly of a transducing-competent Ret complex is suggeste