synergistic anticancer potential of water garlic extract and copper in a human hepatocarcinoma cell line

Garlic extract in water, supplemented with copper, exerts an enhanced anti-proliferative and a caspase-independent apoptotic activity in a human hepatocarcinoma HepG2 cancer cell line. Furthermore, a DNA double strand break downstream, likely responsible for the observed cell death in HepG2 cell, was evidenced by an early over expression of p53 and p21. This observed synergistic antiproliferative and antitumor capability of water garlic extract, in the presence of copper, might be useful as new tool in cancer prevention and therapy

Tariffazione dell’uso delle infrastrutture stradali da parte dei veicoli pesanti: la valutazione d’impatto di politiche a scala europea

La Direttiva Eurovignetta (1999/62/EC e sue successive modifiche) definisce a livello europeo la politica di tariffazione stradale attraverso la regolazione dell’applicazione di tasse, pedaggi e diritti di utenza ai veicoli commerciali che utilizzano la rete di trasporto trans-europea e altre autostrade. Nonostante la Direttiva si collochi lontano nel tempo, il panorama europeo delle tariffe autostradali è ancora oggi discordante e necessita di essere armonizzato. Quest’articolo illustra i principali risultati di un’analisi ex-post sugli impatti della Direttiva Eurovignetta e descrive la metodologia di valutazione seguita per l’analisi ex-ante d’impatti derivanti da future proposte legislative europee

Polarimetric imaging for cancer diagnosis and staging

A medical imaging technique that relies on light polarization could become a fast and accurate optical method for detecting cancer and determining the stage of the disease

Antimicrobial Resistant Staphylococcus Species Colonization in Dogs, Their Owners, and Veterinary Staff of the Veterinary Teaching Hospital of Naples, Italy

This study aimed to identify Staphylococcus species isolated from nasal swabs of both healthy and diseased dogs, and those of human origin, obtained from nasal swabs of both owners and veterinary staff. Firstly, pet owners were requested to complete a questionnaire relating to the care and relationship with their pets, whose results mainly showed a statistically significant higher frequency of hand washing in diseased dogs’ owners than in healthy dogs’ owners. Canine nasal swabs were obtained from 43 diseased dogs and 28 healthy dogs, while human nasal swabs were collected from the respective dogs’ owners (71 samples) and veterinary staff (34 samples). The isolation and identification of Staphylococcus spp. were followed by disk diffusion method to define the antimicrobial resistance profiles against 18 different molecules. Staphylococcus pseudintermedius was the most frequent isolated strain in both diseased (33.3%) and healthy (46.1%) dogs. Staphylococcus epidermidis was the most frequent isolated bacterium in diseased dogs’ owners (66.6%), while in nasal samples of healthy dogs’ owners, the same frequency of isolation (38.4%) was observed for both Staphylococcus epidermidis and Staphylococcus aureus. All the isolated strains showed good susceptibility levels to the tested antimicrobials; however, the carriage of oxacillin-resistant strains was significantly higher in diseased dogs than in healthy ones (71% and 7.7%, respectively). Only in three cases the presence of the same bacterial species with similar antimicrobial resistance profiles in dogs and their owners was detected, suggesting the potential bacterial transmission. In conclusion, this study suggests potential transmission risk of staphylococci from dogs to humans or vice versa, and highlights that the clinical relevance of Staphylococcus pseudintermedius transmission from dog to human should not be underestimated, as well as the role of Staphylococcus aureus from human to dog transmission

Buckling capacity model for timber screws loaded in compression: Experimental, analytical and FE investigations

This paper investigates the buckling of screws loaded in compression inserted into timber members. Screws are often used as a reinforcement in timber structures. However, under compression forces, they are prone to axial buckling. The current model for the screw buckling, enclosed in the EC5 proposal, is based on the general framework of EC3 for the instability of compressed steel members. The main shortcomings of the current formulation for the buckling of screws are the following. (1) The analytical expression for calculating the theoretical buckling load does not follow the observed modes. (2) Due to the need for dedicated studies, the value of the imperfection coefficient is arbitrarily chosen. This paper fills the above gaps. Firstly, a simple analytical expression for predicting the buckling of screws is proposed and validated against experimental and finite element (FE) findings. Furthermore, the formulation adopts a more accurate expression for lateral deformation based on experimental observation. Secondly, a FE model calibrated on experimental tests is used to estimate the defect coefficients of the instability curves as a function of the amplitude of the geometric defects of the screw, expressed as a fraction of its length. Finally, a Markov chain Monte Carlo analysis is carried out to simulate the capacity of screws with different sizes, assuming the uncertainty of all input parameters sampled from suitable probability distributions. The results are used to validate the proposed deterministic capacity model and estimate the uncertainty factors of the design equation

Evaluación mediante teledetección del efecto de canalizaciones sobre el humedal del Saladillo, Argentina

Los humedales son ecosistemas naturales cuya dinámica está determinada principalmente por la presencia de agua. Generalmente se los identifica como áreas que se inundan temporariamente donde el agua subterránea aflora en la superficie, o con áreas con suelos de baja permeabilidad que permanecen cubiertos por agua poco profunda. El agua, por lo tanto, juega un rol fundamental en la determinación de su estructura y funciones ecológicas. Los datos provistos por imágenes satelitales son una herramienta apropiada para analizar humedales y los cambios ocurridos en ellos a lo largo del tiempo. Los Bañados del Saladillo son un complejo de humedales en el centro de Argentina, de gran importancia para la preservación de aves acuáticas. Estos bañados han sufrido profundos cambios por el avance de la agricultura y las canalizaciones realizadas para mitigar el efecto de inundaciones en el sudeste de la provincia de Córdoba de este país. El objetivo del trabajo fue conocer la condición histórica y presente de los Bañados del Saladillo, y analizar los efectos de las canalizaciones sobre los mismos. Se estudiaron 6 imágenes satelitales que se clasificaron con ENVI 4.8 (ITT, 2010), representando la estación seca y húmeda en tres períodos: previo a las canalizaciones (1996); posterior a las canalizaciones (2004-2006); período reciente (2010-2011). Éstas fueron seleccionadas de acuerdo a las precipitaciones acumuladas en 6 meses previos para que fueran comparables. El área de lagunas se redujo entre un 70% (estación húmeda) y un 33% (estación seca). Los mayores cambios observados en la estación húmeda se reflejaron también en la reducción del número de lagunas (40%), la variabilidad de su tamaño (66%), el perímetro de las mismas (28%) y el incremento de la distancia entre ellas. Más allá de la mitigación de las inundaciones, las canalizaciones favorecieron el avance de la agricultura sobre áreas históricamente inundadas en la estación lluviosa, poniendo en riesgo la persistencia de este importante humedal del sudeste de Córdoba en el centro de Argentina. Los resultados obtenidos representan una herramienta útil al momento de tomar decisiones para el manejo y conservación de estos relictos de hábitat natural y su biodiversidad.Wetlands are natural ecosystems whose dynamics is mainly determined by the presence of water. They are generally identified as temporarily flooded areas where groundwater emerges at the surface, or areas with low permeability soils that remain covered by shallow water. The water, therefore, plays a key role in determining the structure and ecological functions of wetlands. Data provided by satellite images are an appropriate tool for analyzing wetlands and their changes over time. The Saladillo Wetlands are a complex of marshlands in the centre of Argentina, of great importance to the preservation of birds. These marshes have suffered profound changes caused by the advancement of agriculture and channels made to mitigate the effects of flooding in the southeast of the province of Cordoba in this country. The objective of our work was to know the historical and present condition of the Saladillo Wetlands, and to analyze the effects of the channelization on them. We studied six satellite images classified with ENVI 4.8. These images represented the dry and the wet season into three periods: prior to the channelization (1996), after the channelization (2004-2006); and recent period (2010- 2011). They were selected according to the accumulated rainfall during the previous 6 months to make them comparable. The pond area was reduced by 70% (wet season) and 33% (dry season). The major changes observed in the wet season also reflected in the reduction in the number of ponds (40%), the variability of their size (66%), the perimeter of flooded areas (28%) and an increasing the distance between ponds. Beyond the flood mitigation, the channelization favoured the advancement of agriculture on areas historically flooded in the rainy season, threatening the persistence of this important wetland of the southeast of Cordoba in central Argentina. Our results represent a useful tool for the management and conservation of these remnants of natural habitat and biodiversity.Fil: Lopez, C.. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; ArgentinaFil: Brandolin, Pablo Germán. Asociación Civil Amigos para la Conservación de las Aves, Córdoba; ArgentinaFil: Campanella, O. R.. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Geologia; ArgentinaFil: Martino, A. L.. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; ArgentinaFil: de Angelo, Carlos Daniel. Universidad Nacional de Misiones. Facultad de Ciencias Forestales. Instituto de Biologia Subtropical - Sede Puerto Iguazu; Argentin

The origins of polarimetric image contrast between healthy and cancerous human colon tissue

International audienceExperimentally measured spectral Mueller matrix images of ex vivo human colon tissue revealed the contrast enhancement between healthy and cancerous zones of colon specimen compared to unpolarized intensity images. Cancer development starts with abnormal changes which being not yet visible macroscopically may alter the polarization of reflected light. We have shown with experiments and modeling that light scattering by small (sub wavelength) scatterers and light absorption (mainly due to blood hemoglobin) are the key factors for observed polarimetric image contrast. These findings can pave the way for the alternative optical technique for the monitoring and early detection of cancer

Genotypic and phenotypic characterization of Escherichia coli isolates recovered from the uterus of mares with fertility problems

Escherichia coli is the bacterial pathogen most frequently associated with mare infertility. Here, we characterized 24 E. coli strains isolated from mares which presented signs of endometritis and infertility from a genotypic and phenotypic point of view. The majority of the isolates belonged to phylogenetic group B1 (9/24, 37.5%). Regarding antibiotic resistance profiles, 10 out of 24 (41.7%) were multidrug-resistant (MDR). Moreover, 17 out of 24 (70.8%) were strong or moderate biofilm producers, and of these eight were MDR strains. Interestingly, 21 out of 24 (87.5%) E. coli strains were phenotypically resistant to ampicillin and 10 of them were also resistant to amoxicillin with clavulanic acid. Regarding the presence of selected virulence factors, 50% of the examined strains carried at least three of them, with fimH detected in all strains, and followed by kpsMTII (11/24, 45.9%). No strain was able to invade HeLa cell monolayers. No relevant differences for all the investigated characteristics were shown by strains that grew directly on plates versus strains requiring the broth-enrichment step before growing on solid media. In conclusion, this work provides new insight into E. coli strains associated with mares' infertility. These results broaden the knowledge of E. coli and, consequently, add useful information to improve prevention strategies and therapeutic treatments contributing to a significant increase in the pregnancy rate in mares

Targeting monocyte chemotactic protein-1 synthesis with bindarit induces tumor regression in prostate and breast cancer animal models

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