L∞-norm and energy quantization for the planar Lane–Emden problem with large exponent

For any smooth bounded domain (Formula presented.), we consider positive solutions to (Formula presented.)which satisfy the uniform energy bound (Formula presented.)for (Formula presented.). We prove convergence to (Formula presented.) as (Formula presented.) of the (Formula presented.)-norm of any solution. We further deduce quantization of the energy to multiples of (Formula presented.), thus completing the analysis performed in De Marchis et al. (J Fixed Point Theory Appl 19:889–916, 2017)

Implementation of pressure reduction valves in a dynamic water distribution numerical model to control the inequality in water supply

The analysis of water distribution networks has to take into account the variability of users’ water demand and the variability of network boundary conditions. In complex systems, e.g. those characterized by the presence of local private tanks and intermittent distribution, this variability suggests the use of dynamic models that are able to evaluate the rapid variability of pressures and flows in the network. The dynamic behavior of the network also affects the performance of valves that are used for controlling the network. Pressure Reduction Valves (PRVs) are used for controlling pressure and reducing leakages. Highly variable demands can produce significant fluctuation of the PRV set point, causing related transient phenomena that propagate through the network and may result in water quality problems, unequal distribution of resources among users, and premature wear of the pipe infrastructure. A model was developed in previous studies and an additional module for pressure control was implemented able to analyze PRVs in a fully dynamic numerical framework. The model was demonstrated to be robust and reliable in the implementation of pressure management areas in the network. The model was applied to a district of the Palermo network (Italy). The district was monitored and pressure as well as flow data were available for model calibration

Three-dimensional numerical simulations on wind- and tide-induced currents: The case of Augusta Harbour (Italy)

The hydrodynamic circulation in the coastal area of the Augusta Bay (Italy), located in the eastern part of Sicily, is analysed. Due to the heavy contamination generated by the several chemical and petrochemical industries active in the zone, the harbour was declared a Contaminated Site of National Interest. To mitigate the risks connected with the industrial activities located near the harbour, it is important to analyse the hydrodynamic circulation in the coastal area. To perform such analysis, a parallel 3D numerical model is used to solve the Reynolds-averaged momentum and mass balance, employing the k-? turbulence model for the Reynolds stresses. The numerical model is parallelized using the programing technology - Message Passing Interface (MPI) and applying the domain decomposition procedure.The Augusta Bay circulation is mainly due to the relative contribution of the wind force acting over the free surface and the tidal motion through the mouths. Due to the geometric complexity of the domain and the presence of several piers along the coast, a curvilinear boundary-fitted computational grid was used, where cells corresponding to land areas or to wharfs were excluded from the computation. Comparisons between numerical results and field measurements were performed. Three different simulations were performed to selectively isolate the effect of each force, wind and tide, acting in the considered domain. The current in the basin was successfully estimated on the basis of the numerical results, demonstrating the specific role of wind and tidal oscillation in the hydrodynamic circulation inside the harbour

Phaseolin expression in tobacco chloroplast reveals an autoregulatory mechanism in heterologous protein translation

Plastid DNA engineering is a well-established research area of plant biotechnology, and plastid transgenes often give high expression levels. However, it is still almost impossible to predict the accumulation rate of heterologous protein in transplastomic plants, and there are many cases of unsuccessful transgene expression. Chloroplasts regulate their proteome at the post-transcriptional level, mainly through translation control. One of the mechanisms to modulate the translation has been described in plant chloroplasts for the chloroplast-encoded subunits of multiprotein complexes, and the autoregulation of the translation initiation of these subunits depends on the availability of their assembly partners [control by epistasy of synthesis (CES)]. In Chlamydomonas reinhardtii, autoregulation of endogenous proteins recruited in the assembly of functional complexes has also been reported. In this study, we revealed a self-regulation mechanism triggered by the accumulation of a soluble recombinant protein, phaseolin, in the stroma of chloroplast-transformed tobacco plants. Immunoblotting experiments showed that phaseolin could avoid this self-regulation mechanism when targeted to the thylakoids in transplastomic plants. To inhibit the thylakoid-targeted phaseolin translation as well, this protein was expressed in the presence of a nuclear version of the phaseolin gene with a transit peptide. Pulse-chase and polysome analysis revealed that phaseolin mRNA translation on plastid ribosomes was repressed due to the accumulation in the stroma of the same soluble polypeptide imported from the cytosol. We suggest that translation autoregulation in chloroplast is not limited to heteromeric protein subunits but also involves at least some of the foreign soluble recombinant proteins, leading to the inhibition of plastome-encoded transgene expression in chloroplast

Blood and sputum biomarkers in COPD and asthma: a review

Chronic obstructive pulmonary disease (COPD) and asthma are lung inflammatory diseases that represent major public health problems. The primary, and often unique, method to evaluate lung function is spirometry, which reflects disease severity rather than disease activity. Moreover, its measurements strictly depend on patient's compliance, physician's expertise and data interpretation. The limitations of clinical history and pulmonary function tests have encouraged focusing on new possible tracers of diseases. The increase of the inflammatory response in the lungs represents an early pathological event, so biological markers related to inflammation may play key roles in earlier diagnosis, evaluation of functional impairment and prognosis. Biomarkers are measurable indicators associated with the presence and/or severity of a biological or pathogenic process, which may predict functional impairment, prognosis and response to therapy. The traditional approach based on invasive techniques (bronchoalveolar lavage and biopsies) may be replaced, at least in part, by using less invasive methods to collect specimens (sputum and blood), in which biomarkers could be measured. Proteomics, by the association between different protein profiles and pathogenic processes, is gaining an important role in pulmonary medicine allowing a more precise discrimination between patients with different outcomes and response to therapy. The aim of this review was to evaluate the use of biomarkers of airway inflammation in the context of both research and clinical practice

Wind- and tide-induced currents in the Stagnone Lagoon (Sicily)

The hydrodynamic circulation is analyzed in the coastal lagoon of Stagnone di Marsala, a natural reserve located in the north-western part of Sicily, using both experimental measurements and numerical simulations. Field measurements of velocities and water levels, carried out using an ultrasound sensor (3D), are used to validate the numerical model. A 3D finite-volume model is used to solve the Reynolds-averaged momentum and mass balance differential equations on a curvilinear structured grid, employing the k–ε turbulence model for the Reynolds stresses. The numerical analysis allows to identify the relative contribution of the forces affecting the hydrodynamic circulation inside the lagoon. In the simulations only wind and tide forces are considered, neglecting the effects of water density changes. Two different conditions are considered. In the first both the wind stress over the free-surface and the tidal motion are imposed. In the second the wind action is neglected, to separately analyze the tide-induced circulation. The comparison between the two test cases highlights the fundamental role of the wind on the hydrodynamics of the Stagnone lagoon, producing a strong vertical recirculation pattern that is not observed when the flow is driven by tides only