Hippocampal apoptosis, neurogenesis suppression, BDNF, TrkB, CREB expression in depressed female and male rats

TARAMAWOSDepression is one of the most important mental disorders affecting work force and quality of life. In depression, signal transduction pathways are disturbed. It has been shown that the expression of proteins responsible for transmission in brain tissue is increased in rats with long-term antidepressants. Gender-related symptom differences in depression are the focus of attention in clinical studies

Determining the Expression Levels of Circulating Tumour Cell Markers in Canine Mammary Tumours

Detection of the circulating tumour cells (CTC) in dogs with a mammary tumour is a useful tool to reveal the micrometastases long before metastases are recognised clinically. The aim of this study was to evaluate the association of the epidermal growth factor receptor (EGFR), claudin 7 (CLND7) and epithelial cell adhesion molecule (EPCAM) with the clinical indices and to reveal the diagnostic importance of these biomarkers in canine mammary tumours (CMTs). Peripheral blood (PB) samples were collected from 45 bitches (group MT) which had single mass with malignant epithelial tumours and 9 healthy bitches (group H). Real time PCR (rt-PCR) was performed to determine the expression levels of EGFR, CLDN7, and EPCAM. Mean values of EGFR and CLDN7 expressions were significantly higher in group MT compared to group H (P < 0.01 and P < 0.001, respectively). The expression level of CLDN7 was positively correlated with EGFR and EPCAM (P < 0.001 and P < 0.05, respectively). The EPCAM expression was associated with increased tumour size (P < 0.05) and EPCAM tended to decrease in the presence of skin ulceration on tumour (P = 0.05). Furthermore, expression levels of EGFR in intact dogs were significantly higher compared to spayed dogs in group MT (P < 0.01). The EGFR expression was significantly higher in the presence of metastases (P < 0.05). Also, increased EGFR was determined in grade 2 compared to grade 1 (P < 0.05). In conclusion, these results show that EGFR, CLDN7, EPCAM markers are measureable in PB and they may provide valuable information about the clinical pathophysiology of CMT

Investigation of the Levels of Copper, Iron, Zinc, Selenium and Ratios of Cu/Se, Cu/Zn, Fe/Se, Fe/Zn in Patients with Hashimoto Thyroiditis

Bu çalışmanın amacı, Hashimoto tiroiditi (HT) etiyopatogenezinde selenyum (Se), demir (Fe), çinko (Zn) ve bakır (Cu) eser elementlerinin serum düzeylerindeki ve oranlarındaki değişimlerinin belirlenmesi ve bu parametreler arasındaki olası ilişkinin değerlendirilmesidir. Sakarya Tıp Dergisi, 2018, 8(2):285-291 ) Gereç ve Yöntem Çalışma 35 HT ve 28 sağlıklı birey ile gerçekleştirildi. Çalışmaya dahil edilen tüm bireylere ait serum Se, Zn, Cu ve Fe düzeyleri indüktif eşleşmiş plazma optik emisyon spektrofotometresi ile ölçüldü Bulgular Serum Se, Fe, Cu ve Zn düzeylerinin HT grubunda kontrol grubuna göre anlamlı derecede azaldığı saptandı (sırasıyla p<0,001, p<0,001, p<0,05 ve p<0,001). Cu/Zn ve Cu/Se oranlarının HT grubunda arttığı görüldü (p<0,05). Ayrıca, HT grubunda Cu düzeyleri ile Cu/Zn oranı; Fe/Se ile Cu/Se oranları; Fe/Zn ile Fe/Se oranları; Fe düzeyleri ile Fe/Se ve Fe/Zn oranları arasında pozitif korelasyon olduğu belirlendi (p<0,01). Se düzeyleri Cu/Se ve Fe/Se oranları ile negatif korelasyon gösterdi (p<0,01). Zn düzeyleri de Cu/Zn oranı ile negatif korelasyon göstermiştir (p<0,01). Sonuç Çalışmamızın sonuçları, eser element düzeyleri ve oranlarının HT etiyopatogenezinde yer aldığını göstermektedir. Hastalığın altta yatan mekanizmaları ile eser element ve oranlarının etkileşiminin aydınlatılması için daha ileri çalışmalara ihtiyaç duyulmaktadırObjective Our aim was to investigate relationship of serum selenium(Se), zinc(Zn), copper(Cu) and iron(Fe) levels and ratios of Cu/Se, Cu/Zn, Fe/Se and Fe/Zn with etiopathogenesis of Hashimoto Thyroiditis (HT). ( Sakarya Med J, 2018, 8(2):285-291 ). Materials and Methods Thirty-fi ve patients with HT and 28 healthy controls were included. Serum Se, Fe, Cu and Zn were measured by inductively coupled plasma optical emission spectrometry (ICP-OES) and Cu/Se, Cu/Zn, Fe/Se and Fe/Zn ratios were calculated. Results Serum selenium, iron, copper and zinc levels were signifi cantly decreased in HT patients group (p<0,001, p<0,001, p<0,05 and p<0,001, respectively). However, Cu/Zn and Cu/Se ratios were signifi cantly increased in HT patients (p<0,05). In HT patients, there were positive correlations between Cu and Cu/Zn; between Fe/Se and Cu/Se ratios; between Fe/Zn and Fe/Se ratios (p<0,01). In the same group, Fe levels were also positively correlated with Fe/Se and Fe/Zn ratios (p<0,01). However, Se level was negatively correlated with Cu/Se and Fe/ Se ratios (p<0,01). Additionally, Zn was negatively correlated with Cu/Zn ratio (p<0,01). Conclusion Our results showed that together with trace element levels and ratios were involved in HT etiopathogenesis. Details of mechanisms underlying the disease should be clarifi ed with further studies

The role of selenoprotein P and selenium in the etiopathogenesis of gestational diabetes mellitus: Association with selenoprotein P1 gene (rs3877899) polymorphism

WOS: 000444761800003Objective: The aim of this study was to investigate the role of selenoprotein P (SeP) and selenium in the etiopathogenesis of gestational diabetes mellitus (GDM) and their association with a common selenoprotein P1 (rs3877899) single nucleotide polymorphism (SNP) in pregnant women with GDM. Materials and methods: Eighty-six pregnant women with GDM and 90 healthy pregnant women from the same geographic region were included in the study. Fasting glucose, insulin HOMA-IR, and HbA1c were compared. Serum selenium levels were measured by graphite-furnace atomic absorption spectrophotometer. Plasma SeP levels were determined by ELISA. Allele-specific polymerase chain reaction (ASPCR) analysis was used to identify polymorphisms of the selenoprotein P1 gene (SEPP1) (rs3877899). Results: The biochemical parameters of GDM such as fasting glucose, insulin, HOMA-IR, and HbA1c were higher in pregnant women with GDM compared to healthy pregnant women. Maternal selenium levels (mu g/L) were 77.99 +/- 7.21 and 76.04 +/- 7.77 in GDM and healthy pregnant women, respectively (p > 0.05). However, SeP levels (ng/mL) were found to be significantly lower in GDM (35.29 +/- 3.00) compared to control subjects (46.98 +/- 4.59) (p < 0.01). Although there was no significant difference in the distribution of the SEPP1 genotypes and alleles between two groups, SeP levels were higher in the GG genotype of the gene compared to their respective control (p < 0.001). Conclusion: Although frequency of SEPP1 polymorphism and selenium levels did not differ significantly between diabetic and healthy pregnant women, SeP levels increased in pregnant women with GDM suggesting SeP plays a role in the etiopathogenesis of GDM. Moreover, the GG genotype of SEPP1 gene polymorphism may be involved in the development of GDM with a different mechanism. It should be clarified with further studies in larger populations

Effect of Breast Cancer and Brast Cancer Treatment on the Blood Serum Concentrations of Trace Elements and Selenoproteins

SciVal Topics Metrics Funding details Abstract Trace elements (TEs) playing critical roles in chemical events that occur at the cellular level in the body are necessary for biological processes in human health. The role of TEs and seleno-proteins and their relationship with breast cancer (BC) have not been studied thoroughly and therefore remain relatively unknown. Our study aimed to investigate possible changes in the serum selenoproteins (Glutathione Peroxidase 1 (GPX1), Glutathione Peroxidase 6 (GPX6), Selenoprotein F (Sel-F), Selenoprotein H (Sel-H), Selenoprotein S (Sel-S), Selenopro-tein V (Sel-V), Selenoprotein M (Sel-M)), and TEs (Se, Zn, Mn, Cu, and Fe) levels, and TEs ratios (Fe/Se, Fe/Zn, Fe/Mn, Cu/Se, Cu/Zn, and Cu/Mn) in patients with BC before and after treatment (surgery, radiotherapy, and chemotherapy), and to evaluate the results in the patient groups with healthy controls. A total of 35 patients with BC and 25 healthy subjects were included in the study. Blood samples were collected from the patient group on the day prior to treatment, and on the day treatment was completed. Serum GPX1, GPX6, Sel-F, Sel-H, and Sel-S levels were decreased in both before and after treatment groups compared to the control. The treatment of BC resulted in increasing the concentration of Sel-V compared to before treatment levels. The treatment of BC resulted in lowering serum Se, Zn, and Fe concentrations compared to before treatment levels. Also, serum Se, Zn, and Fe levels were decreased in both before and after treatment groups compared to the control. The ratios of Cu/Se, Cu/Zn, and Cu/Mn were increased after treatment compared to the values before treatment. Cu/Se and Cu/Zn ratios were increased, but Fe/Mn ratios were decreased after treatment compared to healthy control. This study indicates that changes in serum levels of TEs such as Zn, Mn, Cu, and Se, as well as their ratios and selenoproteins, may be related to the treatments of BC. Further studies are required to clarify the exact specific mechanisms involved in the status of TEs and selenoproteins in therapeutic strategies of BC. © 2022, Polish Society Magnesium Research. All rights reserved