Altitude and vegetation affect soil organic carbon, basal respiration and microbial biomass in apennine forest soils

Both altitude and vegetation are known to affect the amount and quality of soil organic matter (SOM) and the size and activity of soil microbial biomass. However, when altitude and vegetation changes are combined, it is still unclear which one has a greater effect on soil chemical and biochemical properties. With the aim of clarifying this, we tested the effect of altitude (and hence temperature) and vegetation (broadleaf vs pine forests) on soil organic carbon (SOC) and soil microbial biomass and its activity. Soil sampling was carried out in two adjacent toposequences ranging from 500 to 1000ma.s.l. on a calcareous massif in central Italy: one covered only by Pinus nigra J.F. Arnold forests, while the other covered by Quercus pubescens Willd., Ostrya carpinifolia Scop. and Fagus sylvatica L. forests, at 500, 700 and 1000 m a.s.l., respectively. The content of SOC and water-extractable organic carbon (WEOC) increased with altitude for the pine forests, while for the broadleaf forests no trend along the slope occurred, and the highest SOC and WEOC contents were observed in the soil at 700 m under the Ostrya carpinifolia forest. With regard to the soil microbial community, although the size of the soil microbial biomass (Cmic) generally followed the SOC contents along the slope, both broadleaf and pine forest soils showed similar diminishing trends with altitude of soil respiration (\u3a3CO2-C), and \u3a3CO2-C:WEOC and \u3a3CO2-C:Cmic ratios. The results pointed out that, although under the pine forests' altitude was effective in affecting WEOC and SOC contents, in the soils along the broadleaf forest toposequence this effect was absent, indicating a greater impact of vegetation than temperature on SOC amount and pool distribution. Conversely, the similar trend with altitude of the microbial activity indexes would indicate temperature to be crucial for the activity of the soil microbial community

Assessment of water quality and soil salinity in the agricultural coastal plain (Ravenna, North Italy)

To improve knowledge on salt leaching suitability on different soils, in Arenosols and Cambisols croplands in the coastal area of Ravenna (Italy), soil samples were collected in the non-irrigation winter period and irrigation summer period. Concurrently, waters of the canal network were also investigated. Soil samples were analyzed for pH, carbonate, total organic carbon (TOC), particle size distribution, electrical conductivity (EC), bulk density (BD) and water content at field capacity (FC). Water samples were investigated for pH, EC, biological and chemical oxygen demand, sodium adsorption ratio, phosphorus, nitrogen, sulfates and chlorides. All soils had low TOC concentrations and Arenosols showed the lowest clay content, BD and FC. Soils had similar EC values in winter, but in summer the lowest ones were observed in Arenosols, suggesting that irrigation mitigated salinization in Arenosols, while the high clay content, BD and FC prevented or limited the salt leaching in Cambisols. In summer, the increase of total nitrogen and biological oxygen demand, especially in drainage channels, might suggest the leaching of soluble nutrients and organic matter from soils due to the high irrigation water volumes. Finally, our findings stress the need to consider soil type and properties to contrast soil salinization without negative effects on soil C leaching caused by salt leaching practice

Pedodiversity and Organic Matter Stock of Soils Developed on Sandstone Formations in the Northern Apennines (Italy)

Pedodiversity is considered the cornerstone of biodiversity. This work aimed to (1) assess pedodiversity according to vegetation, topographic factors, and lithology and to (2) identify the major soil-forming factors on soil organic matter (SOM) stock at a 0-30 cm depth. These goals were reached using data from 147 georeferenced soil profiles distributed along 400-1000 m (<= 1000) and 1000-2134 m (>1000) altitudinal gradients in the northern part of the Apennine chain in Italy. Soils showed mainly weak or incipient development (i.e., Entisols and Inceptisols), which could be attributed to sand-based lithology, high slope gradients, and low SOM accumulation rates, which promote soil erosion processes. However, higher pedodiversity was observed at >1000 m than at <= 1000 m, likely due to the higher vegetation cover diversity and climate variability; Spodosols and Mollisols were also found. A greater SOM stock was found at >1000 than <= 1000 m, and vegetation seemed to not affect SOM amounts, suggesting a greater influence of climate on SOM content compared to vegetation. Considering ecosystem conservation, the observed spatial pedodiversity could be considered a critical basis for the protection of soil resources and pedodiversity itself in mountain regions

Exploring the canal environment in terms of water, bed sediments and vegetation in a reclaimed floodplain of Northern Italy

The Po plain (Italy) is one of the largest floodplains in Europe that needs environmental restoration. To achieve this goal, the knowledge of the 'environment' (water, bed sediments and vegetation) of the canals crossing such floodplain is necessary. The water flow of the canals was kept low for hydraulic safety purposes from October to March (NIR), and high for irrigation purposes from April to September (IR). Within this framework, this study aimed to assess in 9 sites of the east part of Po plain 1) the canals' environment quality in terms of vegetation diversity, and water and bed sediment physicochemical properties; and 2) how these features are influenced by canal managements and landscape properties. Water was monthly sampled both in NIR and IR periods, the bed sediments were sampled in summer and winter pe-riods, while the vegetation was recorded in spring and autumn. The low water flow during NIR worsened the water quality by increasing the concentrations of nutrients and salts. A higher salt and nutrient concentrations were observed both in water and bed sediments of canals crossing areas with fne texture alluvial deposits than in those flowing through medium texture alluvial deposits. Further, higher nutrient and salt concentrations were observed for the ca-nals used as collectors of the water coming from other canals. Despite the differences observed for the bed sediments and water quality, the vegetation type and biodiversity did not show differences among the study sites probably be-cause affected by the land use of the surrounding landscape. Indeed, the canals cross agricultural land which limit the developments of natural vegetation and do not promote plant biodiversity. Overall, the present study found out the key role of landscape properties and canal managements on 'canal environment' quality which need to be consid-ered to perform an appropriate reclamation of such environments

Performance of the diamond active target prototype for the PADME experiment at the DAΦ\PhiNE BTF

The PADME experiment at the DA$\Phi$NE Beam-Test Facility (BTF) is designed to search for the gauge boson of a new $\rm U(1)$ interaction in the process e$^+$e$^-\rightarrow\gamma$+$\rm A'$, using the intense positron beam hitting a light target. The $\rm A'$, usually referred as dark photon, is assumed to decay into invisible particles of a secluded sector and it can be observed by searching for an anomalous peak in the spectrum of the missing mass measured in events with a single photon in the final state. The measurement requires the determination of the 4-momentum of the recoil photon, performed by a homogeneous, highly segmented BGO crystals calorimeter. A significant improvement of the missing mass resolution is possible using an active target capable to determine the average position of the positron bunch with a resolution of less than 1 mm. This report presents the performance of a real size $\rm (2x2 cm^2)$ PADME active target made of a thin (50 $\mu$m) diamond sensor, with graphitic strips produced via laser irradiation on both sides. The measurements are based on data collected in a beam test at the BTF in November 2015.Comment: 7 pages, 10 figure

The importance of incorporating soil in the life cycle assessment procedure to improve the sustainability of agricultural management

The formidable ability of soil to store carbon has attracted an increasing number of studies, but few of them included soil organic carbon (SOC) sequestration as part of a carbon balance assessment in the agroecosystem. This raises some interesting questions: 1) how orchards conversion increase soil capacity to mitigate the green–house gases (GHG) emissions by storing C? 2) can it be considered in life cycle assessment (LCA)? 3) can SOC pools and soil biochemical properties determination improve LCA interpretation? To answer these questions, this study selected a ten– and fifteen–years–old peach orchards, a twenty–years–old pear orchard, a thirty–years–old kiwi orchard in south-east part of Emilia–Romagna Region (Italy), and a cereals’ field as reference. Soil samples were collected from 0 to 15 and 15–30 cm depths, and the SOC pool amounts (i.e., labile and recalcitrant) determined. LCA was used to estimate the GHG emissions (CO2eq) from the orchards. Results showed that the conversion from cereals to orchard production increased OC stock (+82 % on average) suggesting that orchards cultivation systems have the capacity to enrich soil organic matter. Fertilization had the greatest impact on CO2eq emission accounting for at least 40 % of total CO2eq emissions. Kiwi cultivation had the highest impact on GHG emissions mainly due to the high water and nutrient demand (0.045 and 0.149 kg CO2eq kg−1 fruit yr−1, respectively). When taking into account the C–CO2eq loss by fruit cultivation and C storage in soils, results would indicate that peach and pear orchard agroecosystems promote C sequestration. Conversely, kiwi cultivation showed large CO2eq emissions only partly counterbalanced by SOC sequestration. This study highlights the importance of including soils in LCA: if made mandatory this would allow a wider, yet more detailed, picture of the impact of agricultural practices on C budget. This simple step could help optimise resource management and at the same time improve agroecosystem sustainability

Diamond graphitization by laser-writing for all-carbon detector applications

The surface of a detector grade CVD polycrystalline diamond sample (5 × 5 × 0.05 mm3) was irradiated by an ArF excimer laser (λ = 193 nm, τ = 20 ns) to produce graphitic conductive layers. In particular, two sets of four parallel graphitic strip-like contacts, with 1 mm pitch, were created along the whole sample on the top and on the rear surfaces of the sample respectively. The two series of stripes lie normally to each other. Such a grid allows to obtain a segmented all-carbon device capable of giving bi-dimensional information on particle detection processes in nuclear applications. Afterwards, an extensive characterization of the samples was performed: SEM and micro-Raman investigations to study the morphological and structural evolution of the irradiated areas, EDS measurements to individuate any absorption phenomena from environment associated to laser treatment, and nanoindentation mapping to understand how the hard-soft transformation occurred depending on the locally transferred energy. Finally, current-voltage analyses were carried out checking the ohmic behavior of the diamond-graphite contact. By comparing the results of the different characterization analyses, a strong periodicity of the modified surface properties was found, confirming the reliability and reproducibility of the laser-induced graphitization process. The results demonstrate that the laser-writing technique is a good and fast solution to produce graphitic contacts on diamond surface and therefore represents a promising way to fabricate segmented all-carbon devices

NOXA-Induced Alterations in the Bax/Smac Axis Enhance Sensitivity of Ovarian Cancer Cells to Cisplatin

Ovarian cancer is the most common cause of death from gynecologic malignancy. Deregulation of p53 and/or p73-associated apoptotic pathways contribute to the platinum-based resistance in ovarian cancer. NOXA, a pro-apoptotic BH3-only protein, is identified as a transcription target of p53 and/or p73. In this study, we found that genetic variants of Bcl-2 proteins exist among cisplatin-sensitive and -resistant ovarian cancer cells, and the responses of NOXA and Bax to cisplatin are regulated mainly by p53. We further evaluated the effect of NOXA on cisplatin. NOXA induced apoptosis and sensitized A2780s and SKOV3 cells to cisplatin in vitro and in vivo. The effects were mediated by elevated Bax expression, enhanced caspase activation, release of Cyt C and Smac into the cytosol. Furthermore, gene silencing of Bax or Smac significantly attenuated NOXA and/or cisplatin-induced apoptosis in chemosensitive A2780s cells, whereas overexpression of Bax or addition of Smac-N7 peptide significantly increased NOXA and/or cisplatin-induced apoptosis in chemoresistant SKOV3 cells. To our knowledge, these data suggest a new mechanism by which NOXA chemosensitized ovarian cancer cells to cisplatin by inducing alterations in the Bax/Smac axis. Taken together, our findings show that NOXA is potentially useful as a chemosensitizer in ovarian cancer therapy

Frequency of left ventricular hypertrophy in non-valvular atrial fibrillation

Left ventricular hypertrophy (LVH) is significantly related to adverse clinical outcomes in patients at high risk of cardiovascular events. In patients with atrial fibrillation (AF), data on LVH, that is, prevalence and determinants, are inconsistent mainly because of different definitions and heterogeneity of study populations. We determined echocardiographic-based LVH prevalence and clinical factors independently associated with its development in a prospective cohort of patients with non-valvular (NV) AF. From the "Atrial Fibrillation Registry for Ankle-brachial Index Prevalence Assessment: Collaborative Italian Study" (ARAPACIS) population, 1,184 patients with NVAF (mean age 72 \ub1 11 years; 56% men) with complete data to define LVH were selected. ARAPACIS is a multicenter, observational, prospective, longitudinal on-going study designed to estimate prevalence of peripheral artery disease in patients with NVAF. We found a high prevalence of LVH (52%) in patients with NVAF. Compared to those without LVH, patients with AF with LVH were older and had a higher prevalence of hypertension, diabetes, and previous myocardial infarction (MI). A higher prevalence of ankle-brachial index 640.90 was seen in patients with LVH (22 vs 17%, p = 0.0392). Patients with LVH were at significantly higher thromboembolic risk, with CHA2DS2-VASc 652 seen in 93% of LVH and in 73% of patients without LVH (p <0.05). Women with LVH had a higher prevalence of concentric hypertrophy than men (46% vs 29%, p = 0.0003). Logistic regression analysis demonstrated that female gender (odds ratio [OR] 2.80, p <0.0001), age (OR 1.03 per year, p <0.001), hypertension (OR 2.30, p <0.001), diabetes (OR 1.62, p = 0.004), and previous MI (OR 1.96, p = 0.001) were independently associated with LVH. In conclusion, patients with NVAF have a high prevalence of LVH, which is related to female gender, older age, hypertension, and previous MI. These patients are at high thromboembolic risk and deserve a holistic approach to cardiovascular prevention

Adherence to antibiotic treatment guidelines and outcomes in the hospitalized elderly with different types of pneumonia

Background: Few studies evaluated the clinical outcomes of Community Acquired Pneumonia (CAP), Hospital-Acquired Pneumonia (HAP) and Health Care-Associated Pneumonia (HCAP) in relation to the adherence of antibiotic treatment to the guidelines of the Infectious Diseases Society of America (IDSA) and the American Thoracic Society (ATS) in hospitalized elderly people (65 years or older). Methods: Data were obtained from REPOSI, a prospective registry held in 87 Italian internal medicine and geriatric wards. Patients with a diagnosis of pneumonia (ICD-9 480-487) or prescribed with an antibiotic for pneumonia as indication were selected. The empirical antibiotic regimen was defined to be adherent to guidelines if concordant with the treatment regimens recommended by IDSA/ATS for CAP, HAP, and HCAP. Outcomes were assessed by logistic regression models. Results: A diagnosis of pneumonia was made in 317 patients. Only 38.8% of them received an empirical antibiotic regimen that was adherent to guidelines. However, no significant association was found between adherence to guidelines and outcomes. Having HAP, older age, and higher CIRS severity index were the main factors associated with in-hospital mortality. Conclusions: The adherence to antibiotic treatment guidelines was poor, particularly for HAP and HCAP, suggesting the need for more adherence to the optimal management of antibiotics in the elderly with pneumonia