A comprehensive study of personal and social information use in female brown-headed cowbirds, Molothrus ater

Brood parasites face considerable cognitive challenges when locating and selecting host nests for their young. One aspect of this challenge is determining how to use different sources of information to make decisions regarding the quality of a prospective nest. Here we investigate how female-brown-headed cowbirds, Molothrus ater, use information when prospecting for nests, and then expand upon this to investigate decisions related to foraging. In chapter 1, we demonstrated female could use social information acquired from observing the nest prospecting patterns of conspecifics to influence their own patterns of nest selection. Furthermore, we found a negative relationship between a female’s accuracy at using personal information and her tendency to copy others. In chapter 2, we found the females were able to use social information in a foraging setting as well. The female’s accuracy using personal information remained consistent across nest prospecting and foraging contexts however, the relationship between accuracy and tendency to copy others drastically reversed. A follow up experiment revealed the likely possibility that the differing relationship between personal and social information use depended on the degree of conflict that existed between the two types of information. In chapter 3, we redeveloped and implemented a new RFID tracking technology allowing us to investigate how the cognitive strategies from chapters 1 and 2 translated to a naturalistic, socially complex breeding environment. We found female cowbirds who spent more time prospecting, produced a greater quantity of eggs and demonstrated high accuracy scores during chapter 1 and 2, whereas females who relied on copying others spent significantly less time prospecting and demonstrated lower laying accuracy scores. By demonstrating how individuals’ cognitive strategies relate across context and translate to a socially complex setting, we have demonstrated the importance of examining behaviour in both of these settings and our RFID tracking technology provides researchers with the framework to effectively study this in the future

Environmental Implications of the Foodservice and Food Retail Industries

The growing size and importance of service sector industries in the U.S. economy raises questions about the suitability of the current environmental management system to deal with perhaps a changing set of environmental concerns. This paper analyzes the environmental impacts associated with the activities undertaken and influenced by two service sector industries—foodservice (e.g., restaurants) and food retail (e.g., grocery stores). This paper is not a definitive analysis of the magnitude of the environmental effects of these industries, but is intended to be a comprehensive survey of the types of environmental implications—positive and negative—of these two service sectors. The foodservice and food retail industries are components of a larger industrial system, the food marketing system, that extends from the production of food to the marketing of food products to consumers. The U.S. foodservice industry comprises an estimated 831,000 individual establishments, employs an estimated 11 million people (about 8.6% of the U.S. workforce), and is expected to have total sales of $376 billion in 2000. The U.S. food retail industry encompasses approximately 126,000 grocery stores, employs approximately 3.5 million people (about 2.7$449 billion in 1998. For this analysis, we use a simple conceptual framework that segregates the environmental impacts of these industries into three categories: direct, upstream, and downstream. We conclude that, while the direct environmental impacts (e.g., energy use, solid waste generation; air and water emissions; food safety concerns; refrigerants) of these industries are important to recognize and address, opportunities also exist for these industries to address their upstream and downstream environmental impacts.

Measurement of substrate thermal resistance using DNA denaturation temperature

Heat Transfer and Thermal Management have become important aspects of the developing field of uTAS systems particularly in the application of the the uTAS philosophy to thermally driven analysis techniques such as PCR. Due to the development of flowing PCR thermocyclers in the field of uTAS, the authors have previously developed a melting curve analysis technique that is compatible with these flowing PCR thermocyclers. In this approach a linear temperature gradient is induced along a sample carrying microchannel. Any flow passing through the microchannel is subject to linear heating. Fluorescent monitoring of DNA in the flow results in the generation of DNA melting curve plots. This works presents an experimental technique where DNA melting curve analysis is used to measure the thermal resistance of microchannel substrates. DNA in solution is tested at a number of different ramp rates and the di®erent apparent denaturation temperatures measured are used to infer the thermal resistance of the microchannel substrates. The apparent variation in denaturation temperature is found to be linearly proportional to flow ramp rate. Providing knowledge of the microchannel diameter and a non-varying cross-section in the direction of heat flux the thermal resistance measurement technique is independent of knowledge of substrate dimensions, contact surface quality and substrate composition/material properties. In this approach to microchannel DNA melting curve analysis the difference between the measured and actual denaturation temperatures is proportional to the substrate thermal resistance and the ramp-rate seen by the sample. Therefore quantitative knowledge of the substrate thermal resistance is required when using this technique to measure accurately DNA denaturation temperatur

Off-resonance magnetisation transfer contrast (MTC) MRI using fast field-cycling (FFC)

Peer reviewedPostprin

A hierarchical Bayesian model for predicting ecological interactions using scaled evolutionary relationships

Identifying undocumented or potential future interactions among species is a challenge facing modern ecologists. Recent link prediction methods rely on trait data, however large species interaction databases are typically sparse and covariates are limited to only a fraction of species. On the other hand, evolutionary relationships, encoded as phylogenetic trees, can act as proxies for underlying traits and historical patterns of parasite sharing among hosts. We show that using a network-based conditional model, phylogenetic information provides strong predictive power in a recently published global database of host-parasite interactions. By scaling the phylogeny using an evolutionary model, our method allows for biological interpretation often missing from latent variable models. To further improve on the phylogeny-only model, we combine a hierarchical Bayesian latent score framework for bipartite graphs that accounts for the number of interactions per species with the host dependence informed by phylogeny. Combining the two information sources yields significant improvement in predictive accuracy over each of the submodels alone. As many interaction networks are constructed from presence-only data, we extend the model by integrating a correction mechanism for missing interactions, which proves valuable in reducing uncertainty in unobserved interactions.Comment: To appear in the Annals of Applied Statistic

Nitric oxide production by Biomphalaria glabrata haemocytes: effects of Schistosoma mansoni ESPs and regulation through the extracellular signal-regulated kinase pathway

BACKGROUND: Schistosoma mansoni uses Biomphalaria glabrata as an intermediate host during its complex life cycle. In the snail, the parasite initially transforms from a miracidium into a mother sporocyst and during this process excretory-secretory products (ESPs) are released. Nitric oxide (NO) and its reactive intermediates play an important role in host defence responses against pathogens. This study therefore aimed to determine the effects of S. mansoni ESPs on NO production in defence cells (haemocytes) from schistosome-susceptible and schistosome-resistant B. glabrata strains. As S. mansoni ESPs have previously been shown to inhibit extracellular signal-regulated kinase (ERK) phosphorylation (activation) in haemocytes from susceptible, but not resistant, B. glabrata the regulation of NO output by ERK in these cells was also investigated. RESULTS: Haemocytes from resistant snails challenged with S. mansoni ESPs (20 mug/ml) over 5 h displayed an increase in NO production that was 3.3 times greater than that observed for unchallenged haemocytes; lower concentrations of ESPs (0.1-10 mug/ml) did not significantly increase NO output. In contrast, haemocytes from susceptible snails showed no significant change in NO output following challenge with ESPs at any concentration used (0.1-20 mug/ml). Western blotting revealed that U0126 (1 muM or 10 muM) blocked the phosphorylation (activation) status of ERK in haemocytes from both snail strains. Inhibition of ERK signalling by U0126 attenuated considerably intracellular NO production in haemocytes from both susceptible and resistant B. glabrata strains, identifying ERK as a key regulator of NO output in these cells. CONCLUSION: S. mansoni ESPs differentially influence intracellular NO levels in susceptible and resistant B. glabrata haemocytes, possibly through modulation of the ERK signalling pathway. Such effects might facilitate survival of S. mansoni in its intermediate host

Are Recent Peculiar Velocity Surveys Consistent?

We compare the bulk flow of the SMAC sample to the predictions of popular cosmological models and to other recent large-scale peculiar velocity surveys. Both analyses account for aliasing of small-scale power due to the sparse and non-uniform sampling of the surveys. We conclude that the SMAC bulk flow is in marginal conflict with flat COBE-normalized Lambda-CDM models which fit the cluster abundance constraint. However, power spectra which are steeper shortward of the peak are consistent with all of the above constraints. When recent large-scale peculiar velocity surveys are compared, we conclude that all measured bulk flows (with the possible exception of that of Lauer & Postman) are consistent with each other given the errors, provided the latter allow for `cosmic covariance'. A rough estimate of the mean bulk flow of all surveys (except Lauer & Postman) is ~400 km/s towards l=270, b=0.Comment: 8 pages, 3 figures. To appear in Proceedings of the Cosmic Flows Workshop, Victoria, B. C., Canada, July 1999, eds. S. Courteau, M. Strauss, and J. Willic

Making asset investment decisions for wastewater systems that include sustainability

Effective integrated water management is a key component of the World Water Vision and the way in which aspirations for water equity may be realized. Part of the vision includes the promotion of sustainability of water systems and full accountability for their interaction with other urban systems. One major problem is that “sustainability” remains an elusive concept, although those involved with the provision of urban wastewater systems now recognize that decisions involving asset investment should use the “triple bottom line” approach to society, the economy, and the environment. The Sustainable Water Industry Asset Resource Decisions project has devised a flexible and adaptable framework of decision support processes that can be used to include the principles of sustainability more effectively. Decision mapping conducted at the outset of the project has shown that only a narrow range of criteria currently influence the outcome of asset investment decisions. This paper addresses the concepts of sustainability assessment and presents two case studies that illustrate how multicriteria decision support systems can enhance the assessment of the relative sustainability of a range of options when decisions are being made about wastewater asset investment

Physical model of source region of subduction zone volcanics

The thermal structure of a generic subduction zone is investigated to elucidate the source region of subduction zone volcanics. The steady state thermal field is evaluated for a model subduction zone where the plates are prescribed by kinematic boundary conditions, such that the subducting slab induces a flow in the mantle wedge. The resulting model suggests that the oceanic crust of the downgoing slab is not melted extensively, if at all, and hence is not the source of subduction zone magmatism (with the possible exception of the special case of very young oceanic crust). The temperature in the mantle wedge is high enough to produce melting at the amphibole-buffered peridotite solidus. It is proposed that the combination of vertical motion of water as a free phase and the transport of hydrous phases (e.g., amphiboles) by the slab-induced mantle wedge flow lead to the net transport of water being horizontal, across the mantle wedge from the slab. Provided the subducting oceanic crust enters the asthenosphere at a velocity > 6(±2) cm/yr, the mantle wedge will be hot enough at the limit of the lateral water transport mechanism to generate melting at the amphibole-buffered solidus. The model was then extended to include the effect of localized sources of buoyancy (melt, residue, etc.) as a stationary body force, to investigate the possibility of reversing the slab-induced flow. Best estimates of the buoyancy sources and appropriate viscosity in the wedge suggest that there is likely to be only a weak modulation of the slab-induced flow unless the slab and wedge are locally decoupled, for instance by shear heating, the presence of water, or dehydration/hydration reactions. If there is decoupling, then it is possible for there to be an appreciable reversal of the slab-induced flow. Such an appreciable reversal of flow, if it persists, leads to cooling of the mantle wedge. Hence flow reversal cannot be a steady state mechanism. Instead it would lead to a cycle in the melting with a period of O(1 m.y.). The time dependence of a model with appreciable flow reversal would be reinforced by the need to clear the wedge of infertile material