Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis

The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 A using an in-house Cu Kalpha source and belong to either space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 72.4, c = 241.2 A, and have two molecules of YckF in the asymmetric unit

Ability of an Arterial Waveform Analysis-Derived Hypotension Prediction Index to Predict Future Hypotensive Events in Surgical Patients

BACKGROUND: Intraoperative hypotension is associated with worse perioperative outcomes for patients undergoing major noncardiac surgery. The Hypotension Prediction Index is a unitless number that is derived from an arterial pressure waveform trace, and as the number increases, the risk of hypotension occurring in the near future increases. We investigated the diagnostic ability of the Hypotension Prediction Index in predicting impending intraoperative hypotension in comparison to other commonly collected perioperative hemodynamic variables. METHODS: This is a 2-center retrospective analysis of patients undergoing major surgery. Data were downloaded and analyzed from the Edwards Lifesciences EV1000 platform. Receiver operating characteristic curves were constructed for the Hypotension Prediction Index and other hemodynamic variables as well as event rates and time to event. RESULTS: Two hundred fifty-five patients undergoing major surgery were included in the analysis yielding 292,025 data points. The Hypotension Prediction Index predicted hypotension with a sensitivity and specificity of 85.8% (95% CI, 85.8%-85.9%) and 85.8% (95% CI, 85.8%-85.9%) 5 minutes before a hypotensive event (area under the curve, 0.926 [95% CI, 0.925-0.926]); 81.7% (95% CI, 81.6%-81.8%) and 81.7% (95% CI, 81.6%-81.8%) 10 minutes before a hypotensive event (area under the curve, 0.895 [95% CI, 0.894-0.895]); and 80.6% (95% CI, 80.5%-80.7%) and 80.6% (95% CI, 80.5%-80.7%) 15 minutes before a hypotensive event (area under the curve, 0.879 [95% CI, 0.879-0.880]). The Hypotension Prediction Index performed superior to all other measured hemodynamic variables including mean arterial pressure and change in mean arterial pressure over a 3-minute window. CONCLUSIONS: The Hypotension Prediction Index provides an accurate real time and continuous prediction of impending intraoperative hypotension before its occurrence and has superior predictive ability than the commonly measured perioperative hemodynamic variables

On the Radial and Longitudinal Variation of a Magnetic Cloud: ACE, Wind, ARTEMIS and Juno Observations, Solar Physics

We present observations of the same magnetic cloud made near Earth by the Advance Composition Explorer (ACE), Wind, and the Acceleration, Reconnection, Turbulence and Electrodynamics of the Moon’s Interaction with the Sun (ARTEMIS) mission comprising the Time History of Events and Macroscale Interactions during Substorms (THEMIS) B and THEMIS C spacecraft, and later by Juno at a distance of 1.2 AU. The spacecraft were close to radial alignment throughout the event, with a longitudinal separation of 3.6∘ between Juno and the spacecraft near Earth. The magnetic cloud likely originated from a filament eruption on 22 October 2011 at 00:05 UT, and caused a strong geomagnetic storm at Earth commencing on 24 October. Observations of the magnetic cloud at each spacecraft have been analysed using minimum variance analysis and two flux rope fitting models, Lundquist and Gold–Hoyle, to give the orientation of the flux rope axis. We explore the effect different trailing edge boundaries have on the results of each analysis method, and find a clear difference between the orientations of the flux rope axis at the near-Earth spacecraft and Juno, independent of the analysis method. The axial magnetic field strength and the radial width of the flux rope are calculated using both observations and fitting parameters and their relationship with heliocentric distance is investigated. Differences in results between the near-Earth spacecraft and Juno are attributed not only to the radial separation, but to the small longitudinal separation which resulted in a surprisingly large difference in the in situ observations between the spacecraft. This case study demonstrates the utility of Juno cruise data as a new opportunity to study magnetic clouds beyond 1 AU, and the need for caution in future radial alignment studies

On the Radial and Longitudinal Variation of a Magnetic Cloud : ACE, Wind, ARTEMIS and Juno Observations

We present observations of the same magnetic cloud made near Earth by the Advance Composition Explorer (ACE), Wind, and the Acceleration, Reconnection, Turbulence and Electrodynamics of the Moon's Interaction with the Sun (ARTEMIS) mission comprising the Time History of Events and Macroscale Interactions during Substorms (THEMIS) B and THEMIS C spacecraft, and later by Juno at a distance of 1.2 AU. The spacecraft were close to radial alignment throughout the event, with a longitudinal separation of 3.6 degrees between Juno and the spacecraft near Earth. The magnetic cloud likely originated from a filament eruption on 22 October 2011 at 00:05 UT, and caused a strong geomagnetic storm at Earth commencing on 24 October. Observations of the magnetic cloud at each spacecraft have been analysed using minimum variance analysis and two flux rope fitting models, Lundquist and Gold-Hoyle, to give the orientation of the flux rope axis. We explore the effect different trailing edge boundaries have on the results of each analysis method, and find a clear difference between the orientations of the flux rope axis at the near-Earth spacecraft and Juno, independent of the analysis method. The axial magnetic field strength and the radial width of the flux rope are calculated using both observations and fitting parameters and their relationship with heliocentric distance is investigated. Differences in results between the near-Earth spacecraft and Juno are attributed not only to the radial separation, but to the small longitudinal separation which resulted in a surprisingly large difference in the in situ observations between the spacecraft. This case study demonstrates the utility of Juno cruise data as a new opportunity to study magnetic clouds beyond 1 AU, and the need for caution in future radial alignment studies.Peer reviewe

Trends in adult cardiovascular disease risk factors and their socio-economic patterning in the Scottish population 1995–2008: cross-sectional surveys

<p>Objectives To examine secular and socio-economic changes in cardiovascular disease risk factor prevalences in the Scottish population. This could contribute to a better understanding of why the decline in coronary heart disease mortality in Scotland has recently stalled along with a widening of socio-economic inequalities.</p> <p>Design Four Scottish Health Surveys 1995, 1998, 2003 and 2008 (6190, 6656, 5497 and 4202 respondents, respectively, aged 25–64 years) were used to examine gender-stratified, age-standardised prevalences of smoking, alcohol consumption, physical activity, fruit and vegetable consumption, discretionary salt use and self-reported diabetes or hypertension. Prevalences were determined according to education and social class. Inequalities were assessed using the slope index of inequality, and time trends were determined using linear regression.</p> <p>Results There were moderate secular declines in the prevalence of smoking, excess alcohol consumption and physical inactivity. Smoking prevalence declined between 1995 and 2008 from 33.4% (95% CI 31.8% to 35.0%) to 29.9% (27.9% to 31.8%) for men and from 36.1% (34.5% to 37.8%) to 27.4% (25.5% to 29.3%) for women. Adverse trends in prevalence were noted for self-reported diabetes and hypertension. Over the four surveys, the diabetes prevalence increased from 1.9% (1.4% to 2.4%) to 3.6% (2.8% to 4.4%) for men and from 1.7% (1.2% to 2.1%) to 3.0% (2.3% to 3.7%) for women. Socio-economic inequalities were evident for almost all risk factors, irrespective of the measure used. These social gradients appeared to be maintained over the four surveys. An exception was self-reported diabetes where, although inequalities were small, the gradient increased over time. Alcohol consumption was unique in consistently showing an inverse gradient, especially for women.</p> <p>Conclusions There has been only a moderate decline in behavioural cardiovascular risk factor prevalences since 1995, with increases in self-reported diabetes and hypertension. Adverse socio-economic gradients have remained unchanged. These findings could help explain the recent stagnation in coronary heart disease mortalities and persistence of related inequalities.</p&gt

Field Observations on the Effect of a Mannan Oligosaccharide on Mortality and Intestinal Integrity of Sole (Solea senegalensis, Kaup) Infected by Photobacterium damselae subsp. piscicida

This study was conducted in order to investigate the effect of a mannan oligosaccharide (MOS) on the intestinal morphology of sole (Solea senegalensis, Kaup) reared under commercial conditions. The dietary inclusion rate for MOS was 0.4% and it was used either alone or in combination with a vaccination regime against bacterial diseases (Pasteurella spp. and Vibrio spp.). One week after the start of the experimental period, a natural outbreak of pasteurellosis, caused by Photobacterium damselae subsp.piscicida,occurred in all the groups of fish.A two-way ANOVA showed that only MOS supplementation reduced fish mortality by ca. 8% (P = 0.050). Additionally, light microscopy examination of the intestine revealed that MOS supplementation significantly increased the mucosal folding by 29% (P = 0.016) in the anterior intestinal region and by 33% (P = 0.002) in the posterior intestinal region. Scanning electron microscopy demonstrated that both MOS supplementation and vaccination significantly increased microvilli density on the enterocyte surfaces in the anterior intestinal regionby 13% (P = 0.028) and 30% (P = 0.001) respectively. In the posterior intestinal region neither MOS supplementation nor vaccination significantly affected the microvilli density (P = 0.005).The present study suggests that dietary MOS supplementation protects the intestinal morphology of infected sole and hinders the development of pathogenic infection, possibly by binding with Photobacterium damselae subsp. piscicida, resulting in reduced mortality of infected fish

Longitudinal Growth Following Treatment for Osteosarcoma

Purpose. The purpose of this study was to analyse the height at diagnosis and growth in 72 skeletally immature children who had been treated for osteosarcoma in the area of the knee

{The Noise Handling Properties of the Talbot Algorithm for Numerically Inverting the Laplace Transform}

This paper examines the noise handling properties of three of the most widely used algorithms for numerically inverting the Laplace Transform. After examining the genesis of the algorithms, the regularization properties are evaluated through a series of standard test functions in which noise is added to the inverse transform. Comparisons are then made with the exact data. Our main finding is that the Talbot inversion algorithm is very good at handling noisy data and is more accurate than the Fourier Series and Stehfest numerical inversion schemes as they are outlined in this paper. This offers a considerable advantage for it's use in inverting the Laplace Transform when seeking numerical solutions to time dependent differential equations.Peer reviewedFinal Published versio

Bound States in Mildly Curved Layers

It has been shown recently that a nonrelativistic quantum particle constrained to a hard-wall layer of constant width built over a geodesically complete simply connected noncompact curved surface can have bound states provided the surface is not a plane. In this paper we study the weak-coupling asymptotics of these bound states, i.e. the situation when the surface is a mildly curved plane. Under suitable assumptions about regularity and decay of surface curvatures we derive the leading order in the ground-state eigenvalue expansion. The argument is based on Birman-Schwinger analysis of Schroedinger operators in a planar hard-wall layer.Comment: LaTeX 2e, 23 page

Hamiltonian thermodynamics of a Lovelock black hole

We consider the Hamiltonian dynamics and thermodynamics of spherically symmetric spacetimes within a one-parameter family of five-dimensional Lovelock theories. We adopt boundary conditions that make every classical solution part of a black hole exterior, with the spacelike hypersurfaces extending from the horizon bifurcation three-sphere to a timelike boundary with fixed intrinsic metric. The constraints are simplified by a Kucha\v{r}-type canonical transformation, and the theory is reduced to its true dynamical degrees of freedom. After quantization, the trace of the analytically continued Lorentzian time evolution operator is interpreted as the partition function of a thermodynamical canonical ensemble. Whenever the partition function is dominated by a Euclidean black hole solution, the entropy is given by the Lovelock analogue of the Bekenstein-Hawking entropy; in particular, in the low temperature limit the system exhibits a dominant classical solution that has no counterpart in Einstein's theory. The asymptotically flat space limit of the partition function does not exist. The results indicate qualitative robustness of the thermodynamics of five-dimensional Einstein theory upon the addition of a nontrivial Lovelock term.Comment: 22 pages, REVTeX v3.