Collagen-hyaluronic acid scaffolds for adipose tissue engineering.

Three-dimensional (3-D) in vitro models of the mammary gland require a scaffold matrix that supports the development of adipose stroma within a robust freely permeable matrix. 3-D porous collagen-hyaluronic acid (HA: 7.5% and 15%) scaffolds were produced by controlled freeze-drying technique and crosslinking with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride. All scaffolds displayed uniform, interconnected pore structure (total porosity approximately 85%). Physical and chemical analysis showed no signs of collagen denaturation during the formation process. The values of thermal characteristics indicated that crosslinking occurred and that its efficiency was enhanced by the presence of HA. Although the crosslinking reduced the swelling of the strut material in water, the collagen-HA matrix as a whole tended to swell more and show higher dissolution resistance than pure collagen samples. The compressive modulus and elastic collapse stress were higher for collagen-HA composites. All the scaffolds were shown to support the proliferation and differentiation 3T3-L1 preadipocytes while collagen-HA samples maintained a significantly increased proportion of cycling cells (Ki-67+). Furthermore, collagen-HA composites displayed significantly raised Adipsin gene expression with adipogenic culture supplementation for 8 days vs. control conditions. These results indicate that collagen-HA scaffolds may offer robust, freely permeable 3-D matrices that enhance mammary stromal tissue development in vitro.This was supported by the Biotechnology and Biological Sciences Research Council

Suicide prevention lifeline will listen: a video advertisement

The National Suicide Prevention Lifeline is a 24-hour phone toll-free line primarily dedicated to the prevention of suicide and promoting wellbeing. Perhaps unbeknownst to most of the general public, many callers do not require suicide prevention or crisis intervention, and taking calls from these individuals is still an important aspect of the NSPL. In fact, taking such calls further promotes well-being. The NSPL offers the opportunity for interpersonal connections to everyone, at any time, regardless of whether they are suicidal or not. The NSPL responds to anyone???regardless of gender, age, ethnicity, political beliefs, religion, nationality, or sexual orientation???with the only exception being people who misuse the lifeline in a sexually inappropriate manner. People can call, anytime, for free, and talk to a trained phone counselor who will use active listening skills to make the caller feel heard. This project will be the development of a video advertisement that focuses on the compassionate aspect of the suicide prevention hotline and its broad availability

Parametric face drawings: A demographically diverse and customizable face space model.

We introduce a novel face space model-parametric face drawings (or PFDs)-to generate schematic, though realistic, parameterized line drawings of faces based on the statistical distribution of human facial features. A review of existing face space models (including FaceGen Modeller, Synthetic Faces, MPI, and active appearance model) indicates that current models are constrained by their reliance on ethnically homogeneous face databases. This constraint has led to negative consequences for underrepresented populations, such as impairments in automatized identity recognition of certain demographic groups. Our model is based on a demographically diverse sample of 400 faces (200 female, 200 male; 100 East Asian/Pacific Islander, 100 Latinx/Hispanic, 100 black/African-American, and 100 white/Caucasian) compiled from several face databases (including FERET face recognition technology and the Chicago Face Database). Each front-view face image is manually coded with 85 landmark points that are then normalized and rendered with MATLAB (MathWorks, Natick, MA) tools to produce a smooth, parameterized face line drawing. We present data from two behavioral experiments to validate our model and demonstrate its applicability. In Experiment 1 we show that PFDs produce a reliable "inversion effect" in short-term recognition, a hallmark of holistic processing. In Experiment 2, we conduct a celebrity recognition task, comparing performance on PFDs to performance on untextured renderings from FaceGen Modeller. Participants successfully recognized approximately 50% of celebrity faces based on the PFD models, comparable to performance based on FaceGen Modeler (also 50% correct). We highlight a range of potential applications of our model, list some limitations, and provide MATLAB resources for researchers to utilize our face space, including the ability to customize the demographic makeup of the face space, add new faces, and produce morphs and caricatures

Internal Photoeffect in Films of Poly-N-Epoxypropylcarbazole with High Concentration of Anion Polymethine Dye

Growth of concentration of the anion polymethine dye in the films of poly-N-epoxypropylcarbazole involves increase of quenching of photoluminescence in external electric field as well as appearance of longwave band of the photoluminescence and photoconductivity within visible spectral range. It was ascertained that associates of ionic pairs of the dye arise when the concentration increases. Anomalous for semiconductor materials kinetics of the photocurrent growth and relaxation was observed in the films of these composites: time of the photocurrent growth is much less than the time of its relaxation after the light is switched off. Effect of memory of preliminary illumination with light was observed in the films. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3527

Algorithm of Decision Support in Emergency

Constant increase of human population result in more and more people living in emergency dangerous regions. In order to protect them from possible emergencies we need effective solution for decision taking in case of emergencies, because lack of time for taking decision and possible lack of data. One among possible methods of taking such decisions is shown in this article

A 3-D in vitro co-culture model of mammary gland involution.

Involution is a process whereby the mammary gland undergoes extensive tissue remodelling involving exquisitely coordinated cell death, extracellular matrix degradation and adipose tissue regeneration following the weaning of offspring. These processes are mediated in part through Jak/Stat signalling pathways, which can be deregulated in breast cancer. Synthetic in vitro analogues of the breast could become important tools for studying tumorigenic processes, or as personalized drug discovery platforms and predictors of therapeutic response. Ideally, such models should support 3D neo-tissue formation, so as to recapitulate physiological organ function, and be compatible with high-throughput screening methodologies. We have combined cell lines of epithelial, stromal and immunological origin within engineered porous collagen/hyaluronic acid matrices, demonstrating 3D-specific molecular signatures. Furthermore seeded cells form mammary-like branched tissues, with lobuloalveolar structures that undergo inducible involution phenotypes reminiscent of the native gland under hormonal/cytokine regulation. We confirm that autophagy is mediated within differentiated mammary epithelial cells in a Stat-dependent manner at early time points following the removal of a prolactin stimulus (H/WD). In addition, epithelial cells express markers of an M2 macrophage lineage under H/WD, a process that is attenuated with the introduction of the monocyte/macrophage cell line RAW 264.7. Thus, such 3D models are suitable platforms for studying cell-cell interactions and cell death mechanisms in relation to cancer

Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties.

Chitosan/collagen (Chit/Col) blends have demonstrated great potential for use in tissue engineering (TE) applications. However, there exists a lack of detailed study on the influence of important design parameters (i.e, component ratio or crosslinking methods) on the essential properties of the scaffolds (morphology, mechanical stiffness, swelling, degradation and cytotoxicity). This work entailed a systematic study of these essential properties of three Chit/Col compositions, covering a wide range of component ratios and using different crosslinking methods. Our results showed the possibility of tailoring these properties by changing component ratios, since different interactions occurred between Chit/Col: samples with Chit-enriched compositions showed a hydrogen-bonding type complex (HC), whereas a self-crosslinking phenomenon was induced in Col-enriched scaffolds. Additionally, material and biological properties of the resultant matrices were further adjusted and tuned by changing crosslinking conditions. In such way, we obtained a wide range of scaffolds whose properties were tailored to meet specific needs of TE applications.The authors are grateful to Dr. von Kobbe (Chimera Pharma of Bionostra Group) for the gift of MCF7 cells. The financial support of the Ministerio de Ciencia e Innovación of Spain (FIS PS09/01513), and the FPI grant from UCM to A. Martínez are gratefully acknowledged.This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.carbpol.2015.06.08

Photoconductive Composite Films Based on Cobalt (II) and Nickel (II) Organic Salts Doped with Polymethine Dyes

Photoconducting film compositions based on cobalt (II) and nickel (II) octanoates doped by cationic and(or) anionic polymethine dyes are obtained. Their electric and photoconductive properties are investigated. Anomalous for semiconductor materials peculiarities of the photocurrent were observed in these composites. Effect of memory of preliminary illumination with light was observed in the obtained films. The scheme of non-equilibrium charge carriers photogeneration process in these compositions are suggested. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3528