Effect of Pulsed Electromagnetic Fields (PEMFs) on Muscular Activation during Cycling: A Single-Blind Controlled Pilot Study

Purpose: PEMF stimulation results in a higher O2 muscle supply during exercise through increased O2 release and uptake. Given the importance of oxygen uptake in sport activity, especially in aerobic disciplines such as cycling, we sought to investigate the influence of PEMF on muscle activity when subjects cycled at an intensity between low and severe. Methods: Twenty semi-professional cyclists performed a constant-load exercise with randomized active (ON) or inactive (OFF) PEMF stimulation. Each subject started the recording session with 1 min of cycling without load (warm-up), followed by an instantaneous increase in power, as the individualized workload (constant-load physical effort). PEMF loops were applied on the vastus medialis and biceps femoris of the right leg. We recorded the electromyographic activity from each muscle and measured blood lactate prior the exercise and during the constant-load physical effort. Results: PEMF stimulation caused a significant increase in muscle activity in the warm-up condition when subjects cycled without load (p < 0.001). The blood lactate concentration was higher during PEMF stimulation (p < 0.001), a possible consequence of PEMF's influence on glycolytic metabolism. Conclusion: PEMF stimulation augmented the activity and the metabolism of muscular fibers during the execution of physical exercise. PEMF stimulation could be used to raise the amplitude of muscular responses to physical activity, especially during low-intensity exercise

EFFECTS OF QIGONG ON CELL-FREE MYOSIN PHOSPHORYLATION: PRELIMINARY EXPERIMENTS

This work examines the effect of Qigong from two experienced practitioners on in vitro cellfree myosin phosphorylation. This system has a demonstrated sensitivity to variations in static magnetic fields above and below ambient values. The results show that both Qigong practitioners were able to consistently yield results similar to those observed for variations in applied magnetic fields near the ambient level. Qigong treatment with the myosin reaction mixture in the ambient magnetic field reduced phosphorylation in each experiment by an average of approximately 15% (p < .05). For trials with the myosin samples in a magnetic shield, two of four (one for each practitioner) Qi treatments yielded significant reductions in phosphorylation (average approximately 10%, p < .05). These effects were somewhat lower and not as consistent as those obtained under ambient field conditions. The results obtained in this study demonstrate that Qigong practice can consistently affect a biologically relevant enzyme system. requiring no physical contact between the practitioner and the sample. The mechanisms of the Qigong effects observed in this study are as yet unclear

Non-Thermal Radio Frequency and Static Magnetic Fields Increase Rate of Hemoglobin Deoxygenation in a Cell-Free Preparation

The growing body of clinical and experimental data regarding electromagnetic field (EMF) bioeffects and their therapeutic applications has contributed to a better understanding of the underlying mechanisms of action. This study reports that two EMF modalities currently in clinical use, a pulse-modulated radiofrequency (PRF) signal, and a static magnetic field (SMF), applied independently, increased the rate of deoxygenation of human hemoglobin (Hb) in a cell-free assay. Deoxygenation of Hb was initiated using the reducing agent dithiothreitol (DTT) in an assay that allowed the time for deoxygenation to be controlled (from several min to several hours) by adjusting the relative concentrations of DTT and Hb. The time course of Hb deoxygenation was observed using visible light spectroscopy. Exposure for 10–30 min to either PRF or SMF increased the rate of deoxygenation occurring several min to several hours after the end of EMF exposure. The sensitivity and biochemical simplicity of the assay developed here suggest a new research tool that may help to further the understanding of basic biophysical EMF transduction mechanisms. If the results of this study were to be shown to occur at the cellular and tissue level, EMF-enhanced oxygen availability would be one of the mechanisms by which clinically relevant EMF-mediated enhancement of growth and repair processes could occur

Influence of Electromagnetic Fields On Biological Signalling: An Experimental and Theoretical Approach

The primary goals of this study were to develop a cell-free in vitro assay for the assessment of nonthermal electromagnetic (EMF) bioeffects and to develop theoretical models in accord with current experimental observations. Based upon the hypothesis that EMF effects operate by modulating Ca2+/CaM binding, an in vitro nitric oxide (NO) synthesis assay was developed to assess the effects of a pulsed radiofrequency (PRF) signal used for treatment of postoperative pain and edema. No effects of PRF on NO synthesis were observed. Effects of PRF on Ca2+/CaM binding were also assessed using a Ca2+-selective electrode, also yielding no EMF Ca2+/CaM binding. However, a PRF effect was observed on the interaction of hemoglobin (Hb) with tetrahydrobiopterin, leading to the development of an in vitro Hb deoxygenation assay, showing a reduction in the rate of Hb deoxygenation for exposures to both PRF and a static magnetic field (SMF). Structural studies using pyranine fluorescence, Gd3+ vibronic sideband luminescence and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy were conducted in order to ascertain the mechanism of this EMF effect on Hb. Also, the effect of SMF on Hb oxygen saturation (SO2) was assessed under gas-controlled conditions. These studies showed no definitive changes in protein/solvation structure or SO2 under equilibrium conditions, suggesting the need for real-time instrumentation or other means of observing out-of-equilibrium Hb dynamics. Theoretical models were developed for EMF transduction, effects on ion binding, neuronal spike timing, and dynamics of Hb deoxygenation. The EMF sensitivity and simplicity of the Hb deoxygenation assay suggest a new tool to further establish basic biophysical EMF transduction mechanisms. If an EMF-induced increase in the rate of deoxygenation can be demonstrated in vivo, then enhancement of oxygen delivery may be a new therapeutic method by which clinically relevant EMF-mediated enhancement of growth and repair processes can occur

Inter-Rater Agreement of Biofield Tuning: Testing a Novel Health Assessment Procedure

Objectives: Practitioners of Biofield Tuning assess health status of their clients by detecting off-the-body biofield perturbations using tuning fork (TF) vibrations. This study tested inter-rater agreement (IRA) on location of these perturbations. Design: Three Biofield Tuning practitioners, in randomized order, identified locations of the 4–5 “strongest” perturbations along each of 4 sites for the same series of 10 research subjects. Setting/Location: An Integrative Health and Medicine Center in La Jolla, CA. Subjects: Adult volunteers with no serious current illness and no prior experience of a Biofield Tuning session. Interventions: Practitioners used an activated 174 Hz unweighted TF to “comb” the same four sites per subject, located on the left and right sides of the base of the spine and the heart. Outcome Measures: Practitioners identified and vocalized the distance from the body of perturbations along each site. Distances were recorded by a research assistant in the clinic room. No health information related to perturbation sites was discussed with the subjects. Results: Practitioners reported 6.3 ± 0.6 (mean ± standard deviation) perturbations per combed site per subject, with no significant difference among the raters. The overall level of IRA was low based initially on a first-pass, nonstatistical, analysis of results, with “agreement” defined within a tolerance of ±2 inches. In this approach agreement was 33%. More rigorous statistical analysis, including a statistical test using a Monte Carlo approach, strongly supported the conclusion of poor IRA. Conclusions: IRA was low despite attempts to balance the real-world practice of Biofield Tuning with the constraints of research. For example, while IRA necessitates multiple assessments of the same subject, no information exists as to whether an initial assessment may affect subsequent assessments. Our study exemplifies the challenges faced when attempting to fit interventions with incompletely understood procedures and mechanisms into conventional research designs

Timing of Pulsed Electromagnetic Field Stimulation Does Not Affect the Promotion of Bone Cell Development

Pulsed electromagnetic field (PEMF) devices have been used clinically to promote the healing of surgically resistant fractures in vivo. However, there is a sparsity of data on how the timing of an applied pulsed electromagnetic field effects the osteogenic cells that would be present within the fracture gap. The purpose of this study was to examine the response of osteoblast-like cells to a PEMF stimulus, mimicking that of a clinically available device, using four protocols for the timing of the stimulus. The PEMF signal consisted of a 5ms pulse burst (containing 20 pulses) repeated at 15Hz. Cultures of a human osteosarcoma cell line, SaOS-2, were exposed to the four timing protocols, each conducted over three days. Protocol one stimulated the cells for eight hours each day, protocol two stimulated the cells for 24 hours on the first day, protocol three stimulated the cells for 24 hours on the second day and protocol four stimulated the cells for 24 hours on the third day. Cells were seeded with either 25,000 or 50,000 cells/well (24 well cell culture plates). All assays showed reduced proliferation and increased differentiation (alkaline phosphatase activity) in the PEMF stimulated cultures compared with the control cultures, except for protocol four alkaline phosphatase measurements. No clear trend was observed between the four protocols, however this may be due to cell density. The results indicated that an osteoblast-like cell line is responsive to a 15Hz PEMF stimulus, which will stimulate the cell line to into an increasing state of maturity

Effect of PEMF on Muscle Oxygenation during Cycling: A Single-Blind Controlled Pilot Study

Pulsed electromagnetic fields (PEMFs) are used as non-invasive tools to enhance microcirculation and tissue oxygenation, with a modulatory influence on the microvasculature. This study aimed to measure the acute effect of PEMF on muscle oxygenation and its influence on pulmonary oxygen kinetics during exercise. Eighteen male cyclists performed, on different days, a constant-load exercise in both active (ON) and inactive (OFF) PEMF stimulations while deoxyhemoglobin and pulmonary oxygen kinetics, total oxygenation index, and blood lactate were collected. PEMF enhanced muscle oxygenation, with higher values of deoxyhemoglobin both at the primary component and at the steady-state level. Moreover, PEMF accelerated deoxyhemoglobin on-transition kinetic, with a shorter time delay, time constant, and mean response time than the OFF condition. Lactate concentration was higher during stimulation. No differences were found for total oxygenation index and pulmonary oxygen kinetics. Local application of a precise PEMF stimulation can increase the rate of the muscle O-2 extraction and utilization. These changes were not accompanied by faster oxygen kinetics, reduced oxygen slow component, or reduced blood lactate level. It seems that oxygen consumption is more influenced by exercise involving large muscle mass like cycling, whereas PEMF might only act at the local level

Non-thermal electromagnetic fields increase rate of hemoglobin deoxygenation in a cell-free preparation

The reducing agent dithiothreitol was used to deoxygenate human haemoglobin, and the time course observed via visible light spectroscopy. A decrease in the time required for deoxygenation was found for exposures to both a pulsed radiofrequency signal currently in clinical use for treatment of pain and edema, and a 150 mT static magnetic field