Visualdrives Forensic Tool

Visualdrives is a tool for visualising files stored on a hard drive. The application combines a 3D interactive game-style visualisation combined with a LeapMotion input device providing gesture control over the interface. The purpose of the tool is to understand the potential for abstract digital forensics data to be visualised in a richer, more interactive environment than has traditionally been the case

Observational Evidence of Wave Ducting and Evanescence in the Mesosphere

A collaborative radar and imaging study of gravity waves over the Hawaiian Islands was performed during October 1993 as part of the Airborne Lidar and Observations of Hawaiian Airglow 1993/Coupling and Dynamics of Regions Equatorial (ALOHA-93/CADRE) campaign to investigate the propagation characteristics of short-period (\u3c1 hour) waves at nightglow altitudes. The horizontal wavelengths and apparent phase speeds of quasi-monochromatic wave events were measured in four separate nightglow emissions using data obtained by a high-resolution CCD imager. This information was correlated with simultaneous MF radar wind measurements over the same height interval (∼80–100 km) to infer intrinsic wave parameters in each case. Correlating the two data sets allowed the determination of the local vertical wavenumber for each event, in particular whether it be real (indicative of freely propagating waves) or imaginary (indicative of ducted or evanescent waves). The results of this study indicate a preponderance of ducted or evanescent waves at 80–100 km during the time of the observations, with up to ∼75% of the events recorded exhibiting ducted or evanescent behavior. Also noted was a tendency for ducted behavior to be more prevalent among waves with shorter horizontal wavelengths, in agreement with Doppler ducting theory. These results suggest that ducted waves are relatively common in the upper mesosphere and lower thermosphere region, at least over the mid-Pacific Ocean. As small-scale waves which are ducted have the potential to travel much longer horizontal distances than freely propagating waves, the frequency of their occurrence should be taken into account in efforts to quantify gravity wave effects at these altitudes

Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2

<p>Abstract</p> <p>Background</p> <p>The <it>Arabidopsis thaliana dgat1 </it>mutant, <it>AS11</it>, has an oil content which is decreased by 30%, and a strongly increased ratio of 18:3/20:1, compared to wild type. Despite lacking a functional DGAT1, <it>AS11 </it>still manages to make 70% of WT seed oil levels. Recently, it was demonstrated that in the absence of <it>DGAT1</it>, <it>PDAT1 </it>was essential for normal seed development, and is a dominant determinant in <it>Arabidopsis </it>TAG biosynthesis.</p> <p>Methods</p> <p>Biochemical, metabolic and gene expression studies combined with genetic crossing of selected <it>Arabidopsis </it>mutants have been carried out to demonstrate the contribution of <it>Arabidopsis </it>PDAT1 and LPCAT2 in the absence of DGAT1 activity.</p> <p>Results</p> <p>Through microarray and RT-PCR gene expression analyses of <it>AS11 </it>vs. WT mid-developing siliques, we observed consistent trends between the two methods. <it>FAD2 </it>and <it>FAD3 </it>were up-regulated and <it>FAE1 </it>down-regulated, consistent with the <it>AS11 </it>acyl phenotype. <it>PDAT1 </it>expression was up-regulated by <it>ca </it>65% while <it>PDAT2 </it>expression was up-regulated only 15%, reinforcing the dominant role of <it>PDAT1 </it>in <it>AS11 </it>TAG biosynthesis. The expression of <it>LPCAT2 </it>was up-regulated by 50-75%, while <it>LPCAT1 </it>expression was not significantly affected. <it>In vitro </it>LPCAT activity was enhanced by 75-125% in microsomal protein preparations from mid-developing <it>AS11 </it>seed <it>vs </it>WT. Co-incident homozygous knockout lines of <it>dgat1</it>/<it>lpcat2 </it>exhibited severe penalties on TAG biosynthesis, delayed plant development and seed set, even with a functional PDAT1; the double mutant <it>dgat1/lpcat1 </it>showed only marginally lower oil content than <it>AS11</it>.</p> <p>Conclusions</p> <p>Collectively, the data strongly support that in <it>AS11 </it>it is <it>LPCAT2 </it>up-regulation which is primarily responsible for assisting in PDAT1-catalyzed TAG biosynthesis, maintaining a supply of PC as co-substrate to transfer <it>sn</it>-2 moieties to the <it>sn</it>-3 position of the enlarged <it>AS11 </it>DAG pool.</p

Development of a patient-centred electronic review template to support self-management in primary care

BACKGROUND: Electronic templates are frequently used in long-term condition (LTC) reviews (for example, asthma) to act as reminders and improve documentation; however, they can restrict patient-centred care and opportunities for patients to discuss concerns and self-management. AIM: The IMPlementing IMProved Asthma self-management as RouTine (IMP2ART) programme aimed to develop a patient-centred asthma review template that encourages supported self-management. DESIGN & SETTING: This was a mixed-methods study, which integrated qualitative and systematic review data, primary care Professional Advisory Group feedback, and qualitative data from clinician interviews. METHOD: Aligned with the Medical Research Council complex intervention framework, a template was developed in the following three phases: (1) development phase, which consisted of a qualitative exploration with clinicians and patients, a systematic review, and prototype template development; (2) feasibility pilot phase, which involved feedback from clinicians (n = 7); and (3) pre-piloting phase, which consisted of delivering the template within the IMP2ART implementation strategy (incorporating the template with patient and professional resources) and eliciting clinician feedback (n = 6). RESULTS: Template development was guided by the preliminary qualitative work and the systematic review. A prototype template was developed with an opening question to establish patient agendas, and a closing prompt to confirm agendas have been addressed and an asthma action plan provided. The feasibility pilot identified refinements needed, including focusing the opening question on asthma. Pre-piloting ensured integration with the IMP2ART strategy. CONCLUSION: Following the multi-stage development process, the implementation strategy, including the asthma review template, is now being tested in a cluster randomised controlled trial

Agribusiness management series: Oklahoma Wheat Stocker Purchase Planner

The Oklahoma Cooperative Extension Service periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311

Agribusiness management series: Oklahoma Wheat Stocker Graze Out Decision Aid

The Oklahoma Cooperative Extension Service periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311

Medieval Irish Chronicles Reveal Persistent Volcanic Forcing of Severe Winter Cold Events, 431–1649 CE

Explosive volcanism resulting in stratospheric injection of sulfate aerosol is a major driver of regional to global climatic variability on interannual and longer timescales. However, much of our knowledge of the climatic impact of volcanism derives from the limited number of eruptions that have occurred in the modern period during which meteorological instrumental records are available. We present a uniquely long historical record of severe short-term cold events from Irish chronicles, 431–1649 CE, and test the association between cold event occurrence and explosive volcanism. Thirty eight (79%) of 48 volcanic events identified in the sulfate deposition record of the Greenland Ice Sheet Project 2 ice-core correspond to 37 (54%) of 69 cold events in this 1219 year period. We show this association to be statistically significant at the 99.7% confidence level, revealing both the consistency of response to explosive volcanism for Ireland's climatically sensitive Northeast Atlantic location and the large proportional contribution of volcanism to historic cold event frequencies here. Our results expose, moreover, the extent to which volcanism has impacted winter-season climate for the region, and can help to further resolve the complex spatial patterns of Northern Hemisphere winter-season cooling versus warming after major eruptions.Earth and Planetary Science

Agribusiness management series: Oklahoma Wheat Stocker Spreadsheet Decision Aid

The Oklahoma Cooperative Extension Service periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311

Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses

Viruses have evolved means to manipulate the host's ubiquitin-proteasome system, in order to down-regulate antiviral host factors. The Vpx/Vpr family of lentiviral accessory proteins usurp the substrate receptor DCAF1 of host Cullin4-RING ligases (CRL4), a family of modular ubiquitin ligases involved in DNA replication, DNA repair and cell cycle regulation. CRL4DCAF1 specificity modulation by Vpx and Vpr from certain simian immunodeficiency viruses (SIV) leads to recruitment, poly-ubiquitylation and subsequent proteasomal degradation of the host restriction factor SAMHD1, resulting in enhanced virus replication in differentiated cells. To unravel the mechanism of SIV Vpr-induced SAMHD1 ubiquitylation, we conducted integrative biochemical and structural analyses of the Vpr protein from SIVs infecting Cercopithecus cephus (SIVmus). X-ray crystallography reveals commonalities between SIVmus Vpr and other members of the Vpx/Vpr family with regard to DCAF1 interaction, while cryo-electron microscopy and cross-linking mass spectrometry highlight a divergent molecular mechanism of SAMHD1 recruitment. In addition, these studies demonstrate how SIVmus Vpr exploits the dynamic architecture of the multi-subunit CRL4DCAF1 assembly to optimise SAMHD1 ubiquitylation. Together, the present work provides detailed molecular insight into variability and species-specificity of the evolutionary arms race between host SAMHD1 restriction and lentiviral counteraction through Vpx/Vpr proteins