High-resolution macromolecular crystallography at the FemtoMAX beamline with time-over-threshold photon detection

Protein dynamics contribute to protein function on different time scales. Ultrafast X-ray diffraction snapshots can visualize the location and amplitude of atom displacements after perturbation. Since amplitudes of ultrafast motions are small, high-quality X-ray diffraction data is necessary for detection. Diffraction from bovine trypsin crystals using single femtosecond X-ray pulses was recorded at FemtoMAX, which is a versatile beamline of the MAX IV synchrotron. The time-over-threshold detection made it possible that single photons are distinguishable even under short-pulse low-repetition-rate conditions. The diffraction data quality from FemtoMAX beamline enables atomic resolution investigation of protein structures. This evaluation is based on the shape of the Wilson plot, cumulative intensity distribution compared with theoretical distribution, I/σ, Rmerge /Rmeas and CC1/2 statistics versus resolution. The FemtoMAX beamline provides an interesting alternative to X-ray free-electron lasers when studying reversible processes in protein crystals

Composition en acides amin\ue9s d'os de mammif\ue8res fossiles de deux sites du Plio-P\ue9istoc\ue8ne d'Angola. Comparaison avec la conservation de la phase min\ue9rale

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Eco-conversion of two winery lignocellulosic wastes into fillers for biocomposites: Vine Shoots and Wine Pomaces

Two winery residues, namely vine shoots (ViSh) and wine pomace (WiPo), were up-cycled as fillers in PHBV-based biocomposites. Answering a biorefinery approach, the impact of a preliminary polyphenols extraction step using an acetone/water mixture on the reinforcing effect of fillers was assessed. Biocomposites (filler content up to 20 wt%) were prepared by melt-mixing and compared in terms of final performance (thermal, mechanical and barrier). It was shown that the reinforcing effect was slightly better in the case of vine shoots, while it was not significantly affected by the pre-treatment, demonstrating that these two winery residues could be perfectly used as fillers in composite materials even after an extraction process to maximize their potential of valorization

Language familiarity influences own\u2010race face recognition in 9\u2010 and 12\u2010month\u2010old infants

International audienc

Good Practice Guidelines for Participatory Multi-Site Videoconferencing

This report analyzes recent literature and contributes expertise from researchers and practitioners in the field to develop good practice guidelines for multi-site videoconferencing - linking people in multiple sites with videoconferencing. Our goal was to develop effective, empowering and contextually-sensitive good practice guidelines that encourage participation in multi-site videoconferencing.Ce rapport, qui pr\ue9sente une analyse de publications r\ue9centes ainsi que les avis de chercheurs et de praticiens travaillant sur le terrain, a pour but de mener \ue0 l'\ue9tablissement de lignes directrices relatives aux bonnes pratiques pour les vid\ue9oconf\ue9rences multisite - la mise en contact de personnes r\ue9parties dans diff\ue9rents sites par des vid\ue9oconf\ue9rences. Nous visons \ue0 \ue9tablir des lignes directrices efficaces, utiles et bien adapt\ue9es qui encourageront la participation aux vid\ue9oconf\ue9rences multisite.NRC publication: Ye

Successful hematopoietic stem cell mobilization and apheresis collection using plerixafor alone in sickle cell patients

Novel therapies for sickle cell disease (SCD) based on genetically engineered autologous hematopoietic stem and progenitor cells (HSPCs) are critically dependent on a safe and effective strategy for cell procurement. We sought to assess the safety and efficacy of plerixafor when used in transfused patients with SCD for HSC mobilization. Six adult patients with SCD were recruited to receive a single dose of plerixafor, tested at lower than standard (180 \ub5g/kg) and standard (240 \ub5g/kg) doses, followed by CD34+ cell monitoring in peripheral blood and apheresis collection. The procedures were safe and well-tolerated. Mobilization was successful, with higher peripheral CD34+ cell counts in the standard vs the low-dose group. Among our 6 donors, we improved apheresis cell collection results by using a deep collection interface and starting apheresis within 4 hours after plerixafor administration. In the subjects who received a single standard dose of plerixafor and followed the optimized collection protocol, yields of up to 24.5 7 106 CD34+ cells/kg were achieved. Interestingly, the collected CD34+ cells were enriched in immunophenotypically defined long-term HSCs and early progenitors. Thus, we demonstrate that plerixafor can be employed safely in patients with SCD to obtain sufficient HSCs for potential use in gene therapy

Demographic and Treatment Variables Influencing Outcome for Localized Paratesticular Rhabdomyosarcoma: Results From a Pooled Analysis of North American and European Cooperative Groups

Treatment recommendations for localized paratesticular rhabdomyosarcoma (PT RMS) differ in North America and Europe. We conducted a pooled analysis to identify demographic features and treatment choices that affect outcome

ICTV virus taxonomy profile: Virgaviridae

The family Virgaviridae is a family of plant viruses with rod-shaped virions, a ssRNA genome with a 3\uc2\ua2-terminal tRNA-like structure and a replication protein typical of alpha-like viruses. Differences in the number of genome components, genome organization and the mode of transmission provide the basis for genus demarcation. Tobacco mosaic virus (genus Tobamovirus) was the first virus to be discovered (in 1886); it is present in high concentrations in infected plants, is extremely stable and has been extensively studied. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Virgaviridae, which is available at www.ictv.global/report/virgaviridae