Verminderung gefährlicher Alkaloide in der Nahrungskette durch die züchterische Verbesserung der Mutterkorn-Resistenz von Roggen

Roggen ist als Fremdbefruchter besonders anfällig für einen Befall mit Mutterkorn, der durch eine Infektion mit dem Schadpilz Claviceps purpurea (Fr.) Tul. hervorgerufen wird. Ziele dieser Studie waren die Suche nach effektiven Resistenzträgern gegen Mutterkorn aus genetischen Ressourcen, die Untersuchung der Vererbung von selbstfertilen Roggenmaterialien sowie die Analyse der Wechselwirkungen von Wirtsgenotyp und Mutterkornpilz hinsichtlich der Zusammensetzung und Toxizität der Alkaloide. Zur Untersuchung der Anfälligkeit, von Wirtspflanzen war es notwendig mit CMS (Cytoplasmatic Male-Sterile) Material zu arbeiten. Über drei Jahre wurden an zwei bzw. drei Standorten 75 selbstfertile CMS-Linien und 126 CMS-Einfachkreuzungen sowie 90 Testkreuzungen mit Genetischen Ressourcen deutscher, polnischer und russischer Herkunft. unter ökologischen Bedingungen angebaut, künstlich inokuliert und auf Mutterkornresistenz geprüft. Die Genetischen Ressourcen zeigten nicht in allen Fällen signifikante genotypische Unterschiede. Vor allem im deutschen geprüften Material war keine Signifikanz nachweisbar. Die Linien hatten geringere Mutterkorngewichte als die CMS-Einfachkreuzungen und zeigten immer signifikante genotypische Varianzen, die Einfachkreuzungen in den meisten Fällen. Die Korrelation zwischen beiden Materialgruppen war nur in den ersten beiden Jahren signifikant (r=0,6-0,7; P=0,01). Der mittlere Gesamtalkaloidgehalt der Linien war bei niedrigeren Mutterkorngewichten um ca. 25 % höher als bei den Einfachkreuzungen. Die genotypischen Varianzen für Alkaloide waren bei allen Experimenten und Materialien nicht oder nur schwach signifikant. Die Alkaloidgehalte erwiesen sich als kaum vom geprüften Roggentyp abhängig; aber zeigten starke umweltbedingte Unterschiede. Die Ergebnisse zeigen dass auch im selbstfertilen Material eine gezielte Resistenzselektion möglich ist. Aufgrund quantitativer Vererbung der Resistenz und hoher Genotyp x Umwelt-Wechselwirkungen ist dies allerdings noch ein langer Weg

Habitat selection, facilitation, and biotic settlement cues affect distribution and performance of coral recruits in French Polynesia

Habitat selection can determine the distribution and performance of individuals if the precision with which sites are chosen corresponds with exposure to risks or resources. Contrastingly, facilitation can allow persistence of individuals arriving by chance and potentially maladapted to local abiotic conditions. For marine organisms, selection of a permanent attachment site at the end of their larval stage or the presence of a facilitator can be a critical determinant of recruitment success. In coral reef ecosystems, it is well known that settling planula larvae of reef-building corals use coarse environmental cues (i.e., light) for habitat selection. Although laboratory studies suggest that larvae can also use precise biotic cues produced by crustose coralline algae (CCA) to select attachment sites, the ecological consequences of biotic cues for corals are poorly understood in situ. In a field experiment exploring the relative importance of biotic cues and variability in habitat quality to recruitment of hard corals, pocilloporid and acroporid corals recruited more frequently to one species of CCA, Titanoderma prototypum, and significantly less so to other species of CCA; these results are consistent with laboratory assays from other studies. The provision of the biotic cue accurately predicted coral recruitment rates across habitats of varying quality. At the scale of CCA, corals attached to the “preferred” CCA experienced increased survivorship while recruits attached elsewhere had lower colony growth and survivorship. For reef-building corals, the behavioral selection of habitat using chemical cues both reduces the risk of incidental mortality and indicates the presence of a facilitator

Diabetes mellitus and oral lichen planus: A systematic review and meta-analysis

Objective: To undertake a meta-analysis of the association of Oral Lichen Planus (OLP) with diabetes, two diseases with an important impact on public health and the economy, but the evidence of which about their association is inconsistent. Methods: Relevant studies were localized by searching MEDLINE, EMBASE, Conference Proceedings, and other databases from inception to October 2020, without restrictions. The reference lists of included studies and of related reviews were also inspected. Global pooled odds ratios were calculated, and predefined subgroup analyses were performed. The heterogeneity between studies and publication bias was assessed and sensitivity analysis was carried out. Results: Thirty-two studies were included in the meta-analysis. Pooled ORs showed a moderate association between diabetes and OLP [OR: 1.87 (95%CI: 1.57, 2.34)]. The association is limited to studies carried out on adults only [OR: 2.12 (95%CI: 1.75, 2.57)] and is observed in all study designs. Globally, the heterogeneity was low to moderate. Studies carried out in European populations show a stronger association of diabetes and OLP than Asiatic studies [OR: 2.49 (95%CI: 1.87, 3.32) and 1.60 (95%CI: 1.25, 2.03), respectively]. Conclusions: Diabetes and OLP are moderately associated. Systematic diagnosis of diabetes in OLP patients could prove usefulS

Addressing vulnerability, building resilience:community-based adaptation to vector-borne diseases in the context of global change

Abstract Background The threat of a rapidly changing planet – of coupled social, environmental and climatic change – pose new conceptual and practical challenges in responding to vector-borne diseases. These include non-linear and uncertain spatial-temporal change dynamics associated with climate, animals, land, water, food, settlement, conflict, ecology and human socio-cultural, economic and political-institutional systems. To date, research efforts have been dominated by disease modeling, which has provided limited practical advice to policymakers and practitioners in developing policies and programmes on the ground. Main body In this paper, we provide an alternative biosocial perspective grounded in social science insights, drawing upon concepts of vulnerability, resilience, participation and community-based adaptation. Our analysis was informed by a realist review (provided in the Additional file 2) focused on seven major climate-sensitive vector-borne diseases: malaria, schistosomiasis, dengue, leishmaniasis, sleeping sickness, chagas disease, and rift valley fever. Here, we situate our analysis of existing community-based interventions within the context of global change processes and the wider social science literature. We identify and discuss best practices and conceptual principles that should guide future community-based efforts to mitigate human vulnerability to vector-borne diseases. We argue that more focused attention and investments are needed in meaningful public participation, appropriate technologies, the strengthening of health systems, sustainable development, wider institutional changes and attention to the social determinants of health, including the drivers of co-infection. Conclusion In order to respond effectively to uncertain future scenarios for vector-borne disease in a changing world, more attention needs to be given to building resilient and equitable systems in the present

Final Report for FRDC Project 1998/306

Early life history of abalone (Haliotis rubra, H. laevigata): settlement, survival and early growt

Growth and survival of juvenile Greenlip Abalone (Halitosis laevigata) feeding on germlings of the macroalgae Ulva sp

Germlings of the green alga Ulva sp. were developed as a diet for juvenile Haliotis laevigata ("653.5 mm shell length) and compared with a current commercial diet consisting of Ulvella lens plus the diatom species Navicula cf. jeffreyi. The utilization of macroalgae germlings (juvenile gametophyte and sporophyte) allowed 3-dimensional growth and subsequently provided greater feed biomass in comparison with the current 2-dimensional commercial feed for the later nursery phase consisting of 5 1310 mm (shell length) juvenile abalone. The juvenile abalone showed active feeding on both the Ulva germling diet and the current commercial diet. The Ulvella lens/Navicula cf. jeffreyi diet resulted in abalone of significantly larger shell length at the end of the 14-wk feeding trial. However, the Ulva germling diet recorded significantly larger abalone for the first 4 135 wk, whereas the commercial diet produced significantly larger abalone from week 6 to the end of the trial. The growth rate on both diets exceeded 100 ;Cm.day"121 and the specific growth rates were maintained above 1%.day"121 for the duration of the feeding trial with neither measure portraying significant differences between diets. There was no significant difference in juvenile abalone mortality feeding on the two diets. The Ulva germling consumption exhibited a spike (500 germling blades.abalone"121.day"121) in consumption at week three then, once reduced, a gradual increase occurred until the end of the trial. Ulvella lens consumption demonstrated a similar pattern to Ulva germlings consumption and was significantly, positively correlated. Consumption rates for the two green algae both correlated with juvenile abalone growth. The diatom (Navicula cf. jeffreyi) consumption was affected by plate rotation (light intensity and grazing pressure) rather than juvenile abalone

Changes in amino acid content of an algal feed species (Navicula sp.) and their effect on growth and survival of juvenile abalone (Haliotis rubra)

The growth and survival of juvenile Haliotis rubra, when fed with the diatom Navicula sp. cultured in f/2 medium containing combined nitrogen at 24.71 mg NO3-N L-1 (high), 12.35 mg NO3-N L-1 (standard) or 2.47 mg NO3-N L-1 (low), were compared in a 33-day trial. The alga in the low nitrogen medium contained 37% less total amino acid than that in the high and standard nitrogen media. There was a slightly greater reduction in essential amino acids (40%) compared to non-essential amino acids (35%). Juvenile abalone feeding on Navicula grown in medium with low nitrate and lower total amino acid content grew more slowly than when fed on the same species grown in standard or higher nitrogen medium with a higher amino acid content. The growth rate of juveniles was highest (43 μm d-1) in the high nitrate treatment followed (40 μm d-1) by the standard nitrate treatment and lowest (31 μm d-1) in the low nitrate treatment. The survival of the juveniles was also effected by the diet. Survival was better in the high and standard nitrogen media (88%) than the low nitrogen medium (75%). The results suggest that in order to achieve uniformity in nutritional quality of diatoms and good growth of abalone juveniles in commercial abalone nurseries, the nitrogen concentration in tanks should be monitored and additional nitrate added to provide an optimum concentration of between 2 and 12 mg NO3-N L-1

Changes in amino acid content of an algal feed species (Navicula sp.) and their effect on growth and survival of juvenile abalone (Haliotis rubra)

The growth and survival of juvenile Haliotis rubra, when fed with the diatom Navicula sp. cultured in f/2 medium containing combined nitrogen at 24.71 mg NO3-N L-1 (high), 12.35 mg NO3-N L-1 (standard) or 2.47 mg NO3-N L-1 (low), were compared in a 33-day trial. The alga in the low nitrogen medium contained 37% less total amino acid than that in the high and standard nitrogen media. There was a slightly greater reduction in essential amino acids (40%) compared to non-essential amino acids (35%). Juvenile abalone feeding on Navicula grown in medium with low nitrate and lower total amino acid content grew more slowly than when fed on the same species grown in standard or higher nitrogen medium with a higher amino acid content. The growth rate of juveniles was highest (43 μm d-1) in the high nitrate treatment followed (40 μm d-1) by the standard nitrate treatment and lowest (31 μm d-1) in the low nitrate treatment. The survival of the juveniles was also effected by the diet. Survival was better in the high and standard nitrogen media (88%) than the low nitrogen medium (75%). The results suggest that in order to achieve uniformity in nutritional quality of diatoms and good growth of abalone juveniles in commercial abalone nurseries, the nitrogen concentration in tanks should be monitored and additional nitrate added to provide an optimum concentration of between 2 and 12 mg NO3-N L-1