Evolution-guided functional analyses reveal diverse antiviral specificities encoded by IFIT1 genes in mammals.

IFIT (interferon-induced with tetratricopeptide repeats) proteins are critical mediators of mammalian innate antiviral immunity. Mouse IFIT1 selectively inhibits viruses that lack 2'O-methylation of their mRNA 5' caps. Surprisingly, human IFIT1 does not share this antiviral specificity. Here, we resolve this discrepancy by demonstrating that human and mouse IFIT1 have evolved distinct functions using a combination of evolutionary, genetic and virological analyses. First, we show that human IFIT1 and mouse IFIT1 (renamed IFIT1B) are not orthologs, but are paralogs that diverged >100 mya. Second, using a yeast genetic assay, we show that IFIT1 and IFIT1B proteins differ in their ability to be suppressed by a cap 2'O-methyltransferase. Finally, we demonstrate that IFIT1 and IFIT1B have divergent antiviral specificities, including the discovery that only IFIT1 proteins inhibit a virus encoding a cap 2'O-methyltransferase. These functional data, combined with widespread turnover of mammalian IFIT genes, reveal dramatic species-specific differences in IFIT-mediated antiviral repertoires

Invertebrate carcasses as a resource for competing Aedes albopictus and Aedes aegypti (Diptera : Culicidae)

However, little is known of the role of these carcasses in other containers, which also receive leaf fall and stemflow inputs. This experiment investigated effects of accumulated invertebrate carcasses as a resource for two competing mosquitoes, Aedes albopictus (Skuse) and Aedes aegypti (L.) whether either species differentially benefited from accumulated carcasses, and if such a benefit affected interspecific competition. First, we measured accumulation of invertebrate carcasses in standard containers at a field site. We then used a replacement series with five different species ratios at the same total density: and varied the input of invertebrate carcasses [dead Drosophila melanogaster (Meigen) ] in three levels: none, the average input from our field site, or the maximum input recorded at our field site. Survivorship, development time, and mass were measured for each mosquito species as correlates of population growth, and were used to calculate a population performance index, lambda\u27. There were strong positive effects of invertebrate carcass additions on all growth correlates and lambda\u27. Differences in performance between species were pronounced in small or no carcass additions and absent in large inputs of invertebrate carcasses, but there was little evidence that inputs of invertebrate carcasses altered the competitive advantage in this system. These results suggest that terrestrial invertebrate carcasses may be an important resource for many types of container communities, and large accumulations of dead invertebrates may: reduce resource competition between these mosquitoes. thus favoring coexistence. We propose that the total amount of resource, including accumulated invertebrate carcasses, may explain observed patterns of replacement involving these mosquitoes

Cornering characteristics of the main-gear tire of the space shuttle orbiter

An experimental investigation was conducted at the NASA Langley Research Center to study the effects of various vertical load and yaw angle conditions on the cornering behavior of the Space Shuttle Orbiter main gear tire. Measured parameters included side and drag force, side and drag force coefficients, aligning torque, and overturning torque. Side force coefficient was found to increase as yaw angle was increased, but decreased as the vertical load was increased. Drag force was found to increase as vertical load was increased at constant yaw angles. Aligning torque measurements indicated that the tire is stable in yaw

Electron-Positron Jets from a Critically Magnetized Black Hole

The curved spacetime surrounding a rotating black hole dramatically alters the structure of nearby electromagnetic fields. The Wald field which is an asymptotically uniform magnetic field aligned with the angular momentum of the hole provides a convenient starting point to analyze the effects of radiative corrections on electrodynamics in curved spacetime. Since the curvature of the spacetime is small on the scale of the electron's Compton wavelength, the tools of quantum field theory in flat spacetime are reliable and show that a rotating black hole immersed in a magnetic field approaching the quantum critical value of $B_k=m^2 c^3/(e\hbar) \approx 4.4 \times 10^{13}$~G $\approx 1.3\times10^{-11}$ cm$^{-1}$ is unstable. Specifically, a maximally rotating three-solar-mass black hole immersed in a magnetic field of $2.3 \times 10^{12}$~G would be a copious producer of electron-positron pairs with a luminosity of $3 \times 10^{52}$ erg s$^{-1}$.Comment: 10 pages, 6 figures, submitted to Phys. Rev.

Very-high-energy gamma radiation associated with the unshocked wind of the Crab pulsar

We show that the relativistic wind in the Crab pulsar, which is commonly thought to be invisible in the region upstream of the termination shock at R < 0.1 pc, in fact could be directly observed through its inverse Compton gamm-ray emission. The search for such specific component of radiation in the gamma-ray spectrum of the Crab can provide unique information about the unshocked pulsar wind that is not accessible at other wavelengths.Comment: 11 pages, 11 figures, to appear in one of the April issues of MNRA

Subjective cognitive decline predicts lower cingulo-opercular network functional connectivity in individuals with lower neurite density in the forceps minor

Cognitive complaints of attention/concentration problems are highly frequent in older adults with subjective cognitive decline (SCD). Functional connectivity in the cingulo-opercular network (CON-FC) supports cognitive control, tonic alertness, and visual processing speed. Thus, those complaints in SCD may reflect a decrease in CON-FC. Frontal white-matter tracts such as the forceps minor exhibit age- and SCD-related alterations and, therefore, might influence the CON-FC decrease in SCD. Here, we aimed to determine whether SCD predicts an impairment in CON-FC and whether neurite density in the forceps minor modulates that effect. To do so, we integrated cross-sectional and longitudinal analyses of multimodal data in a latent growth curve modeling approach. Sixty-nine healthy older adults (13 males; 68.33 ± 7.95 years old) underwent resting-state functional and diffusion-weighted magnetic resonance imaging, and the degree of SCD was assessed at baseline with the memory functioning questionnaire (greater score indicating more SCD). Forty-nine of the participants were further enrolled in two follow-ups, each about 18 months apart. Baseline SCD did not predict CON-FC after three years or its rate of change (p-values > 0.092). Notably, however, the forceps minor neurite density did modulate the relation between SCD and CON-FC (intercept; b = 0.21, 95% confidence interval, CI, [0.03, 0.39], p = 0.021), so that SCD predicted a greater CON-FC decrease in older adults with relatively lower neurite density in the forceps minor. The neurite density of the forceps minor, in turn, negatively correlated with age. These results suggest that CON-FC alterations in SCD are dependent upon the forceps minor neurite density. Accordingly, these results imply modifiable age-related factors that could help delay or mitigate both age and SCD-related effects on brain connectivity

Unstable states in QED of strong magnetic fields

We question the use of stable asymptotic scattering states in QED of strong magnetic fields. To correctly describe excited Landau states and photons above the pair creation threshold the asymptotic fields are chosen as generalized Licht fields. In this way the off-shell behavior of unstable particles is automatically taken into account, and the resonant divergences that occur in scattering cross sections in the presence of a strong external magnetic field are avoided. While in a limiting case the conventional electron propagator with Breit-Wigner form is obtained, in this formalism it is also possible to calculate $S$-matrix elements with external unstable particles.Comment: Revtex, 7 pages. To appear in Phys. Rev. D53(2

New Neighbours: Modelling the Growing Population of Gamma-ray Millisecond Pulsars

The Fermi Large Area Telescope, in collaboration with several groups from the radio community, have had marvellous success at uncovering new gamma-ray millisecond pulsars (MSPs). In fact, MSPs now make up a sizable fraction of the total number of known gamma-ray pulsars. The MSP population is characterized by a variety of pulse profile shapes, peak separations, and radio-to-gamma phase lags, with some members exhibiting nearly phase-aligned radio and gamma-ray light curves (LCs). The MSPs' short spin periods underline the importance of including special relativistic effects in LC calculations, even for emission originating from near the stellar surface. We present results on modelling and classification of MSP LCs using standard pulsar model geometries.Comment: 4 pages, 2 figures, proceedings of the ICREA Workshop on The High-Energy Emission from Pulsars and their Systems (HEEPS), Sant Cugat, Spai