17 research outputs found
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Mechanisms affecting the bioaccumulation of dietary quinoline by rainbow trout (Salmo gairdneri)
Factors that influenced the uptake, storage, and elimination of dietary quinoline by rainbow trout (Salmo gairdneri) were studied to obtain an understanding of the mechanisms affecting the bioaccumulation of dietary contaminants in teleosts. Rainbow trout readily absorbed ¹⁴C-quinoline from pelleted food (1% ration at 138 ug quinoline/g food) and most tissues reached apparent steady-state after 10 days feeding. Maximum whole-body concentrations of quinoline plus metabolites were only 30 ng/g after 7 days depuration. Uptake rate constants ranged from 0.00006/day for muscle to 0.1455/day for gallbladder plus bile. Mean elimination half-life for quinoline-derived radioactivity ranged from 0.4 days in gills to 8.7 days for muscle. Depending on tissue, 58-83% of the stored radioactivity was present as metabolites. About 14% of the radioactivity in the bile was present as glucuronide conjugates. Quinoline was absorbed from the stomach by rainbow trout and peak serum levels occurred 4-8 hr after a single feeding. Pharmacokinetics were described using a two-compartment body model with first-order absorption and disposition; estimated half-lives for the a and B phase were 4.1 and 54.1 hr, respectively. Depending on dose, 71 to 83% of the ingested radioactivity was excreted during the first 24 hr after feeding. Branchial excretion was the primary route of excretion, all other routes (fecal, biliary, urinary, dermal) contributing 99% of quinoline was available for absorption. About 60% of the residual body burden was stored in the gallbladder bile, but bile was retained only in starved fish. There was no evidence for enterohepatic circulation of quinoline or its metabolites following ejection of gallbladder bile. Increased feeding rates enhanced the movement of the food bolus and associated radioactivity through the intestine, but did not affect patterns of tissue disposition
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The Independent Technical Analysis Process
The Bonneville Power Administration (BPA) contracted with the Pacific Northwest National Laboratory (PNNL) to provide technical analytical support for system-wide fish passage information (BPA Project No. 2006-010-00). The goal of this project was to produce rigorous technical analysis products using independent analysts and anonymous peer reviewers. In the past, regional parties have interacted with a single entity, the Fish Passage Center to access the data, analyses, and coordination related to fish passage. This project provided an independent technical source for non-routine fish passage analyses while allowing routine support functions to be performed by other well-qualified entities
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Chromium Toxicity Test for Fall Chinook Salmon (Oncorhynchus tshawytscha) Using Hanford Site Groundwater: Onsite Early Life-Stage Toxicity Evaluation
The objective of this study was to evaluate site-specific effects for early life-stage (eyed eggs to free swimming juveniles) fall chinook salmon that might be exposed to hexavalent chromium from Hanford groundwater sources. Our exposure conditions included hexavalent chromium obtained from Hanford groundwater wells near the Columbia River, Columbia River water as the diluent, and locally adapted populations of fall chinook salmon. This report describes both a 96-hr pretest using rainbow trout eggs and an early life-stage test beginning with chinook salmon eggs
DOE Hydropower Program Biennial Report for FY 2005-2006
SUMMARY The U.S. Department of Energy (DOE) Hydropower Program is part of the Office of Wind and Hydropower Technologies, Office of Energy Efficiency and Renewable Energy. The Program's mission is to conduct research and development (R&D) that will increase the technical, societal, and environmental benefits of hydropower. The Department's Hydropower Program activities are conducted by its national laboratories: Idaho National Laboratory (INL) [formerly Idaho National Engineering and Environmental Laboratory], Oak Ridge National Laboratory (ORNL), Pacific Northwest National Laboratory (PNNL), and National Renewable Energy Laboratory (NREL), and by a number of industry, university, and federal research facilities. Programmatically, DOE Hydropower Program R&D activities are conducted in two areas: Technology Viability and Technology Application. The Technology Viability area has two components: (1) Advanced Hydropower Technology (Large Turbine Field Testing, Water Use Optimization, and Improved Mitigation Practices) and (2) Supporting Research and Testing (Environmental Performance Testing Methods, Computational and Physical Modeling, Instrumentation and Controls, and Environmental Analysis). The Technology Application area also has two components: (1) Systems Integration and Technology Acceptance (Hydro/Wind Integration, National Hydropower Collaborative, and Integration and Communications) and (2) Supporting Engineering and Analysis (Valuation Methods and Assessments and Characterization of Innovative Technology). This report describes the progress of the R&D conducted in FY 2005-2006 under all four program areas. Major accomplishments include the following: Conducted field testing of a Retrofit Aeration System to increase the dissolved oxygen content of water discharged from the turbines of the Osage Project in Missouri. Contributed to the installation and field testing of an advanced, minimum gap runner turbine at the Wanapum Dam project in Washington. Completed a state-of-the-science review of hydropower optimization methods and published reports on alternative operating strategies and opportunities for spill reduction. Carried out feasibility studies of new environmental performance measurements of the new MGR turbine at Wanapum Dam, including measurement of behavioral responses, biomarkers, bioindex testing, and the use of dyes to assess external injuries. Evaluated the benefits of mitigation measures for instream flow releases and the value of surface flow outlets for downstream fish passage. Refined turbulence flow measurement techniques, the computational modeling of unsteady flows, and models of blade strike of fish. Published numerous technical reports, proceedings papers, and peer-reviewed literature, most of which are available on the DOE Hydropower website. Further developed and tested the sensor fish measuring device at hydropower plants in the Columbia River. Data from the sensor fish are coupled with a computational model to yield a more detailed assessment of hydraulic environments in and around dams. Published reports related to the Virtual Hydropower Prospector and the assessment of water energy resources in the U.S. for low head/low power hydroelectric plants. Convened a workshop to consider the environmental and technical issues associated with new hydrokinetic and wave energy technologies. Laboratory and DOE staff participated in numerous workshops, conferences, coordination meetings, planning meetings, implementation meetings, and reviews to transfer the results of DOE-sponsored research to end-users
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Biological Assessment of the Advanced Turbine Design at Wanapum Dam, 2005
This report summarizes the results of studies sponsored by the U.S. Department of Energy and conducted by Pacific Northwest National Laboratory to evaluate the biological performance (likelihood of injury to fish) from an advanced design turbine installed at Unit 8 of Wanapum Dam on the Columbia River in Washington State in 2005. PNNL studies included a novel dye technique to measure injury to juvenile fish in the field, an evaluation of blade-strike using both deterministic and stochastic models, and extended analysis of the response of the Sensor Fish Device to strike, pressure, and turbulence within the turbine system. Fluorescein dye was used to evaluate injuries to live fish passed through the advanced turbine and an existing turbine at two spill discharges (15 and 17 kcfs). Under most treatments the results were not significantly different for the two turbines, however, eye injury occurred in nearly 30% of fish passing through Unit 9 but in less than 10% of those passing through Unit 8 at 15 kcfs. Both deterministic and stochastic blade-strike models were applied for the original and new AHTS turbines. The modeled probabilities were compared to the Sensor Fish results (Carlson et al. 2006) and the biological studies using juvenile fish (Normandeau et al. 2005) under the same operational parameters. The new AHTS turbine had slightly higher modeled injury rates than the original turbine, but no statistical evidence to suggest that there is significant difference in blade-strike injury probabilities between the two turbines, which is consistent with the experiment results using Sensor Fish and juvenile fish. PNNL also conducted Sensor Fish studies at Wanapum Dam in 2005 concurrent with live fish studies. The probablility of severe collision events was similar for both turbine. The advanced turbine had a slightly lower probability of severe shear events but a slightly higher probability of slight shear
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Laboratory Studies on the Effects of Shear on Fish
The overall objective of our studies was to specify an index describing the hydraulic force that fish experience when subjected to a shear environment. Fluid shear is a phenomenon that is important to fish. However, elevated levels of shear may result in strain rates that injure or kill fish. At hydroelectric generating facilities, concerns have been expressed that strain rates associated with passage through turbines, spillways, and fish bypass systems may adversely affect migrating fish. Development of fish friendly hydroelectric turbines requires knowledge of the physical forces (injury mechanisms) that impact entrained fish and the fish's tolerance to these forces. It requires up-front, pre-design specifications for the environmental conditions that occur within the turbine system, in other words, determining or assuming that those conditions known to injure fish will provide the descriptions of conditions that engineers must consider in the design of a turbine system. These biological specifications must be carefully and thoroughly documented throughout the design of a fish friendly turbine. To address the development of biological specifications, we designed and built a test facility where juvenile fish could be subjected to a range of shear environments and quantified their biological response
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Evaluation of Water Quality Conditions Near Proposed Fish Production Sites Associated with the Yakima Fisheries Project, 1991-1993 Final Report.
In 1991, the Pacific Northwest Laboratory (PNL) began studying water quality at several sites in the Yakima River Basin for the Bonneville Power Administration. These sites were being proposed as locations for fish culture facilities as part of the Yakima Fisheries Project (YFP). Surface water quality parameters near the proposed fish culture facilities are currently suitable for fish production. Water quality conditions in the mainstream Yakima River and its tributaries are generally excellent in the upper part of the watershed (i.e., near Cle Elum), but they are only fair to poor for the river downstream of Union Gap (river mile 107). Water quality of the Naches River near Oak Flats is also suitable for fish production. Groundwater supplies near the proposed fish production facilities typically have elevated concentrations of metals and dissolved gases. These conditions can be mitigated using best engineering practices such as precipitation and degasification. Additionally, mixing with surface water may improve these conditions. Depending on the location and depth of the well, groundwater temperatures may be warmer than optimum for acclimating and holding juvenile and adult fish. Water quality parameters measured in the Yakima River and tributaries sometimes exceed the range of values described as acceptable for culture of salmonids and for the protection of other aquatic life. However, constituent concentrations are within ranges that exist in many northwest fish hatcheries. Additionally, site-specific tests conducted by PNL (i.e., live box exposures and egg incubation studies) indicate that fish can be successfully reared in surface and well water near the proposed facility sites. Thus, there appear to be no constraints to artificial production for the YFP
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Optimizing Dam Operations for Power and for Fish: an Overview of the US Department of Energy and US Army Corps of Engineers ADvanced Turbine Development R&D. A Pre-Conference Workshop at HydroVision 2006, Oregon Convention Center, Portland, Oregon July 31, 2006
This booklet contains abstracts of presentations made at a preconference workshop on the US Department of Energy and US Army Corps of Engineers hydroturbine programs. The workshop was held in conjunction with Hydrovision 2006 July 31, 2006 at the Oregon Convention Center in Portland Oregon. The workshop was organized by the Corps of Engineers, PNNL, and the DOE Wind and Hydropower Program. Presenters gave overviews of the Corps' Turbine Survival Program and the history of the DOE Advanced Turbine Development Program. They also spoke on physical hydraulic models, biocriteria for safe fish passage, pressure investigations using the Sensor Fish Device, blade strike models, optimization of power plant operations, bioindex testing of turbine performance, approaches to measuring fish survival, a systems view of turbine performance, and the Turbine Survival Program design approach
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Evidence of Deepwater Spawning of Fall Chinook Salmon (Oncorhynchus Tshawytscha) : Spawning Near Ives and Pierce Island of the Columbia River, 1999.
Fall chinook salmon Oncorhynchus tshawytscha, thought to originate from Bonneville Hatchery, were first noted to be spawning downstream of Bonneville Dam by Washington Department of Fisheries and Wildlife (WDFW) biologists in 1993 (Hymer 1997). Known spawning areas include gravel beds on the Washington side of the river near Hamilton Creek and Ives island. Limited spawning ground surveys were conducted in the area around Ives and Pierce Islands during 1994-1997 and based on these surveys it was believed that fall chinook salmon successfully spawned in this area. The size of this population from 1994 to 1996 was estimated at 1,800 to 5,200 fish (Hymer 1997). Recently, chum salmon were also documented spawning downstream of Bonneville Dam. Chum salmon O. kisutch were listed as threatened under the Endangered Species Act (ESA) in March, 1999. There are several ongoing investigations to define the physical habitat characteristics associated with fall chinook and chum salmon spawning areas downstream of Bonneville Dam. A major concern is to determine what flows (i.e. surface elevations) are necessary to ensure their long-term survival. Our objective was to locate deepwater spawning locations in the main Columbia River channel and to collect additional data on physical habitat parameters at the site. This objective is consistent with the high priority that the Northwest Power Planning Council's Independent Advisory Board and the salmon managers have placed on determining the importance of mainstem habitats to the production of salmon in the Columbia River Basin
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Hatchery Effectiveness Technical Work Group Retreat Proceedings, January 9-11, 1990.
This report summarizes a retreat held for the Hatchery Effectiveness Technical Work Group (HETWG). The objectives were to improve the effectiveness of the Technical Work Group (TWG) through developing procedures for its operation, and to develop an action plan for revision of their current research plan