Effects of MRI on sex hormones and other fertility parameters in adult male rats

زمینه و هدف: از بهترین تکنیک های دنیای پزشکی در تشخیص بیماری ها استفاده از تصویربرداری رزونانس مغناطیسی (MRI) است. برای تولید تصاویر در MRI از سه نوع میدان الکترومغناطیسی متفاوت استفاده می شود. هدف از این مطالعه بررسی اثرات میدان های MRI بر ترشح هورمون های جنسی و سایر پارامترهای باروری در موش های صحرایی نر بالغ بوده است. مطالعات متعددی در مورد اثرات هر یک از این میدان ها بر سیستم های زیستی وجود دارد. روش بررسی: در این مطالعه تجربی 40 سر موش صحرایی نر بالغ نژاد ویستار به صورت تصادفی به چهار گروه 10 تایی (دو گروه آزمایشی و دو گروه کنترل) تقسیم شدند. گروه های آزمایش به مدت 25 دقیقه در کویل دستگاه MRI با شدت میدان مغناطیسی 35/0 تسلا قرار گرفتند. سپس حیوانات دوره اول آزمایش و کنترل را به سرعت در هولدر قرار داده و دمای پوست اسکرتوم آنها با دما سنج دیجیتالی اندازه گیری شد. این حیوانات به آزمایشگاه منتقل و پس از خونگیری و تهیه نمونه های سرم، کشته شدند. بیضه ها از بدن آنها خارج و با ترازوی دیجیتالی وزن شد. 96 ساعت بعد از MRI همین مراحل برای حیوانات دوره دوم آزمایش و کنترل انجام شد. هورمون های تستوسترون، هورمون تحریک کننده فولیکول (FSH) و هورمون لوتئیزه کننده (LH) به روش رادیوایمنواسی (RIA) اندازه گیری و در گروه های مختلف با هم مقایسه شدند. یافته ها: در مرحله اول که پس از انجام MRI صورت گرفت، افزایش معناداری در هورمون FSH (003/0=P) و کاهش معنی داری در میزان هورمون تستوسترون گروه آزمایش نسبت به کنترل مشاهده شد (001/0=P) اما تغییرات میزان هورمون LH، وزن بیضه ها و دمای پوست اسکرتوم معنی دار نبود. در مورد دوره دوم که 96 ساعت بعد از انجام MRI صورت گرفت فقط افزایش معنی دار هورمون FSH در گروه آزمایش نسبت به کنترل که مشابه نتایج دوره اول بود مشاهده شد (04/0=P). نتیجه گیری: میدان های MRI می توانند باعث اختلال در ترشح برخی از هورمون های جنسی موش صحرایی نر بالغ شوند

Download citationShare Download full-text PDF A comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) assay in assessment of antiproliferation effect of bromelain on 4T1, AGS and PC3 cancer cell lines

Background: All natural anticancer agents are cytotoxic basically and act mainly by the inhibition cell proliferation; but they have different mechanisms. Two assays, thiazolyl blue [3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyl-terazoliumbromide or MTT] and sulforhodamine B (SRB), are used to assess cell growth. This study aimed to compare measurements between MTT and SRB on the cancer cell lines. Methods: Different concentrations of the bromelain were added to cultured cells including mouse breast cancer (4T1), human gastric carcinoma (AGS), and human prostate carcinoma (PC3) cell lines and incubated at 24 and 48 hours. The growth and proliferation rates of the studied cells were investigated using both MTT and SRB assays after treatment with bromelain. The differences between cells were determined using Kruskal-Wallis and Dunns tests. Findings: Bromelain significantly decreased growth and proliferation rate of 4T1, AGS and PC3 cancer cells, in a concentration-dependent manner at different times, in both MTT and SRB assays. Conclusion: Findings showed that both MTT and SRB assays gained similar data regardless of the cell types. A comparison of Thiazolyl blue (MTT) versus Sulforhodamine B (SRB) assay in assessment of antiproliferation effect of bromelain on 4T1, AGS and PC3 cancer cell lines (PDF Download Available). Available from: https://www.researchgate.net/publication/320563601_A_comparison_of_Thiazolyl_blue_MTT_versus_Sulforhodamine_B_SRB_assay_in_assessment_of_antiproliferation_effect_of_bromelain_on_4T1_AGS_and_PC3_cancer_cell_lines [accessed Nov 07 2017]

Synthesis and Application of new Gadolinium-Porphyrins as Poterntial MR Imaging Contrast Agents for Cancer Detection in Nude Mice

ABSTRACT Two new potential magnetic resonance imaging contrast agents, Gd-hematoporphyrin (Gd-H) and Gd-tetra-carboranylmethoxyphenyl-porphyrin (Gd-TCP), were synthesized and applied to nude mice with human melanoma (MM-138) xenografts. These agents showed a high relaxivity because of their greater potential to coordinate water molecules. The reduction of T 1 relaxation times of 16 and 21% was observed in human melanoma tumors grafted in the nude mice 24 h after injection of Gd-TCP andGd-H, respectively. The percent of injected Gd, that localized to the tumor and measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES), was approximately 21% for Gd-TCP and 28% for Gd-H. A higher concentration of Gd was achieved compared with control indicating selective delivery of Gd-porphyrins to the melanoma xenografts. The data indicate that Gd-TCP can be used as a dual probe for diagnosis in MR imaging and for therapy in boron neutron capture therapy (BNCT). Iran. Biomed. J. 5 (2 & 3): 87-95, 200

The radioprotective effect of resveratrol against genotoxicity induced by γ-irradiation in mice blood lymphocytes

In this study, we evaluated whether the protective potential of resveratrol (RSV; 3,5,4'-trihydroxy-trans-stilbene) against γ-radiation caused damages in peripheral blood lymphocyte of mice. Resveratrol as a polyphenolic compound scavenges free radicals. Various doses of RSV were administered intraperitoneally 2 hours to adult male mice before a single dose of whole-body γ-irradiation (2 Gy). To assess the protective ability of RSV, the alkaline comet assay in blood lymphocyte of mice was performed and the total comet score was evaluated. The results of the alkaline comet assay showed that RSV significantly inhibited radiationinduced DNA damage. We observed that RSV protects blood lymphocyte against radiation-induced damage in mice

Functionalized Magnetic Nanoparticles for the Detection And Quantitative Analysis of CellSurfaceAntigen

Cellsurfaceantigensasbiomarkersoffertremendouspotentialforearlydiagnosis,prognosis,andtherapeuticresponseinavariety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic cell separation principle was applied to identify and quantitatively analyze the cell surface antigenexpressioninthecaseofprostatecancercells.Comparingthecapabilityoftheassaywith�owcytometryasagoldstandard methodshowedsimilarresults.eresultsshowedthattheantigen-speci�cmagneticcellseparationwithantibody-coatedmagnetic nanoparticleshashighpotentialforquantitativecellsurfaceantigendetectionandanalysi

Cytotoxicity effect of pineapple extract on breast cancer cells (4T1)

Aqueous extract of pineapple (Bromelain) is used in medicine. Bromelain has anticancer effect on leukemia, lymphoma, sarcoma and melanoma. The purpose of this study was to evaluate the anticancer effect of bromelain on mouse breast cancer (4T1) cells under in-vitro conditions. Methods: Mouse breast cancer (4T1) cells were incubated with different concentrations of the bromelain for 2, 24, 48 and 72 hours. The growth-inhibitory was investigated via 3 (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay. Kruskal-Wallis and Dunn’s tests were used to evaluate the statistical significance of the difference between the experimental and control groups. Findings: Bromelain significantly inhibited proliferation of 4T1 cells in a concentration-dependent manner. In all concentrations, cell viability decreased with respect to the control incubated cells in the absence of extract (P < 0.05). Bromelain exerted highest antiproliferative effects on 4T1 cells for concentrations of 100, 200, 300, 400 and 600 µg/ml (P < 0.05) for 24, 48 and 72 hours, while cell viability increased for times shorter than 2 hours. The half maximal inhibitory concentration (IC50) value of 142.6 g/ml was obtained from treating 4T1 cells. Conclusion: Results showed that bromelain has cytotoxic effects on the growth and proliferation of 4T1 cells for 24, 48 and 72 hours of incubation. This study is ongoing to assess the extract mechanism of action

Evaluation of cumulative radiation dose in neonates in neonatal surgery ward of Alzahra Hospital of Isfahan

Introduction: The purpose of this study, is evaluation of the average of cumulative radiation exposure in admitted neonates in neonatal surgery ward.Materials and methods: Participants were all neonates consecutively admitted to the neonatal surgery unit of the study hospital for surgery and need one type of radiological study during hospitalization. Thermo luminescent dosimeters (TLD GR200) were used for evaluating absorbed dose of radiation on the body. For controlling of confounding variables, 130 admitted neonates who need no radiation were selected as control group.Results: In this study, we evaluated 169 neonates.The most x -ray examination was on ribcage (338 cases). Total amount of bowel and backbone x-ray examinations were 117 and 11 respectively. Total amount of contrast enema, meal and swallow was 8, 9 and 5 respectively. The cumulative absorbed dose in 19 patients was more than 10 mSv. There was a significant differences between control group with other x ray examination groups (p=0.001). The mean of accumulated received dose for patients during admission period was 3.13±5.12 mSv.Conclusion: The average of accumulated received dose for neonates was about 3.13 mSv. Although this dose is less than annual limit dose, but it is inacceptable in comparison with other medical centers

Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker

Objective(s): The central role of molecular imaging modalities in cancer management is an undeniable fact that could help to diagnose cancer tumors in early stages. The main aim of this study is to prepare a novel targeted molecular imaging nanoprobe of CD24-PEGylated Au NPs to improve the ability of Computed tomography scanning (CT scan) outputs for both in vitro and in vivo detection of breast cancer (4T1) cells. Materials and methods: Gold nanoparticles (Au NPs) were synthesized and coated with polyethylene glycol (PEG) chains in order to improve the stability of the Au NPs and to provide bio modification points for antibody immobilization. The synthesized nanoprobe was used for both in vitro and in vivo targeted CT imaging breast cancer cells (4T1) and the xenograft tumor model. Results: Findings showed that the attenuation coefficient of 4T1 cells that were targeted by CD24-PEGylated Au NPs is calculated to be over two times higher than the untargeted 4T1 cells (15 HU vs 39 HU, respectively). Indeed, the results clearly reveal that the developed CD24-PEGylated Au NPs showed the tumor CT enhancement was higher than that of Omnipaqe which used as control. Also, the CT number values of the tumor area at different time points gradually increased after 60 min post injection and was significantly higher than before injection. Conclusions: Results showed the introduced CT imaging nanoprobe (Au NPs-PEGylated) could be useful for CT imaging of breast tumors under in vivo condition. Overall, it is concluded that Au NPs-PEGylated contrast media is able to detect breast cancer (4T1) cells and is more effective compared with other casual methods

The Rationality to Requesting in-ward Magnetic Resonance Imaging Investigation

Background: Magnetic Resonance imaging (MRI) is a valuable diagnostic tool by its non-invasive/non-ionizing nature.Objective: This study aims to determine justification of MRI in hospitalized patients at a tertiary provincial referent medical center in a one-year period.Material and Methods: In the present retrospective and descriptive cross-sectional study, 438 admitted patients referred for MRI during 2017 were selected using systematic random sampling. The age, gender, investigated organ, the specialty of requesting physician, MRI with and without contrast, MRI diagnostic outcome were collected using checklists. Descriptive statistics and chi-square test were used for data analysis.Results: The mean age of the patients was 42±26 years-old and female represented 53% of enrolled patients. The most and less prevalent investigated organs were the cerebrum and the orbit. After excluding cancer diagnosis, cancer staging, and therapeutic follow-up exams, MRI request was oriented in 64.3% and 77.2% of positive results was concordant with aforementioned diagnostic orientation (P<0.001). Oriented diagnostic MRI requesting is influenced by age, medical specialists and, investigated organ (P<0.001). The positive MRI is influenced significantly by oriented MRI request, gender, medical specialists and investigated organ (P<0.001). The diagnosis concordance of MRI is influenced significantly by oriented MRI request, medical specialists and investigated organ (P<0.001). Conclusion: Appropriate implementation of medical imaging requires boosting employed rationality by the concerned physicians. The current suboptimal results to requesting MRI rationality should mandate supplementary educational programs as to incite the medical corpus more closely implementing the published medical practice guidelines

Anti-CD24 bio Modified PEGylated Gold Nanoparticles as Targeted Computed Tomography Contrast Agent

Purpose: Molecular imaging is one of the import methods for recognition of cancer at the early stage in order to enhance the capacity of remedy. This study was aimed to introduce a new contrast agent that was targeted with CD24 so as to improve the CT scan detection of cancer cells with higher CD24 expression. Methods: The surface modifications of gold nanoparticles (Au-NPs) were done with long PEG (HS-PEG-CH3O) and short PEG (HS-PEG-COOH) chains to enhance their stability and capacity for immobilization of different antibodies. MTT assay was carried out to assess the biocompatibility of the NPs. The obtained contrast agent was implemented in the targeted CT imaging based on in vitro and in vivo studies of breast cancer. Results: The results revealed that the attached CD24 to the cell surface of PEGylated Au-NPs could enhance significantly the cells CT number (40.45 HU in 4T1, while it was 16.61 HU in CT26) It was shown that the attenuation coefficient of the molecularly targeted cells was more than 2 times excessive than the control groups. Further, the tumor region in model of xenograft tumor has higher density compare to the omnipaque groups, 60 min after injection (45 Hu vs.81 Hu). These results showed that the nanoparticles stayed in tumor region for longer time. Conclusion: It is predicted that the synthesized nanoparticle can be used as computed tomography contrast agent. Also, it can be used to identify the tumor cells with higher expression of CD24 at the early stages more efficiently compare to the other routine methods