14 research outputs found
Trophic Cascades, Nutrients, and Lake Productivity: Whole-Lake Experiments
Responses of zooplankton, pelagic primary producers, planktonic bacteria, and CO2 exchange with the atmosphere were measured in four lakes with contrasting food webs under a range of nutrient enrichments during a seven-year period. Prior to enrichment, food webs were manipulated to create contrasts between piscivore dominance and planktivore dominance. Nutrient enrichments of inorganic nitrogen and phosphorus exhibited ratios of N:P \u3e 17:1, by atoms, to maintain P limitation. An unmanipulated reference lake, Paul Lake, revealed baseline variability but showed no trends that could confound the interpretation of changes in the nearby manipulated lakes. Herbivorous zooplankton of West Long Lake (piscivorous fishes) were large-bodied Daphnia spp., in contrast to the small-bodied grazers that predominated in Peter Lake (planktivorous fishes). At comparable levels of nutrient enrichment, Peter Lake\u27s areal chlorophyll and areal primary production rates exceeded those of West Long Lake by factors of approximately three and six, respectively. Grazers suppressed pelagic primary producers in West Long Lake, relative to Peter Lake, even when nutrient input rates were so high that soluble reactive phosphorus accumulated in the epilimnions of both lakes during summer. Peter Lake also had higher bacterial production (but not biomass) than West Long Lake. Hydrologic changes that accompanied manipulation of East Long Lake caused concentrations of colored dissolved organic carbon to increase, leading to considerable variability in fish and zooplankton populations. Both trophic cascades and water color appeared to inhibit the response of primary producers to nutrients in East Long Lake. Carbon dioxide was discharged to the atmosphere by Paul Lake in all years and by the other lakes prior to nutrient addition. During nutrient addition, only Peter Lake consistently absorbed CO2 from the atmosphere, due to high rates of carbon fixation by primary producers. In contrast, CO2 concentrations of West Long Lake shifted to near-atmospheric levels, and net fluxes were near zero, while East Long Lake continued to discharge CO2 to the atmosphere
Botulinum Neurotoxin Devoid of Receptor Binding Domain Translocates Active Protease
Clostridium botulinum neurotoxin (BoNT) causes flaccid paralysis by disabling synaptic exocytosis. Intoxication requires the tri-modular protein to undergo conformational changes in response to pH and redox gradients across endosomes, leading to the formation of a protein-conducting channel. The ∼50 kDa light chain (LC) protease is translocated into the cytosol by the ∼100 kDa heavy chain (HC), which consists of two modules: the N-terminal translocation domain (TD) and the C-terminal Receptor Binding Domain (RBD). Here we exploited the BoNT modular design to identify the minimal requirements for channel activity and LC translocation in neurons. Using the combined detection of substrate proteolysis and single-channel currents, we showed that a di-modular protein consisting only of LC and TD was sufficient to translocate active protease into the cytosol of target cells. The RBD is dispensable for cell entry, channel activity, or LC translocation; however, it determined a pH threshold for channel formation. These findings indicate that, in addition to its individual functions, each module acts as a chaperone for the others, working in concert to achieve productive intoxication
Predicting Lake Erie Algal Blooms based on Alternate Ecosystem States Theory: Early Warning Signals of an Impending Bloom
Algal blooms have become a yearly occurrence in Lake Erie for some time now. These blooms are not only a nuisance but can also pose a risk to human health. Theoretically, early warning signals will exist prior to a shift in ecosystem state, i.e. an algal bloom. Indicators, such as increasing variance and rising autocorrelation close to 1, have been associated with transitions to alternate ecosystem states. These early warning signals have been observed in some whole-ecosystem experiments using quickest detection (QD) methods. The goal of this study was to determine if these early warning signals were detected in chlorophyll data prior to an algal bloom in a real-life ecosystem, Lake Erie. The QD method for detecting early warning signals associated with shifts in ecosystem states was used. In Lake Erie, the shift from a mixed phytoplankton state to a cyanobacterial dominated state was considered a transition to an alternate ecosystem state. Results showed that increasing variance before an algal bloom was not always detected, and therefore early warning signals of an impending algal bloom were not seen. The research suggests that examining phycocyanin, a pigment specific to blue-green algae, may provide more promising results in the future. If successful, this research could be used to provide warnings of impeding algal blooms to water treatment managers, allowing them to be prepared for the situation
Evidence for net nitrogen gas production in Lake Erie surface waters
Two methods are commonly employed to quantify rates of N2-fixation in surface waters. Acetylene reduction assays (ARA) are an indirect measure of N2-fixation, relying on the conversion of acetylene to ethylene at a known mole ratio with respect to dissolved nitrogen gas. An alternative method, membrane-inlet-mass-spectrometry (MIMS), can directly quantify dissolved N2 gas concentrations in water.Here, surface sample grabs (0.5m depth) were collected at 6 sites in Sandusky Bay on 4 dates (N=24) and bioassays were performed using both techniques. Significant levels of N2-fixation were detected with ARA in 83% of samples, whereas net N2-fixation was not detected in any samples (0%) by analysis via MIMS. In contrasts, significant levels of N2-production were observed in 40% of the samples. N2-production is not possible to detect with ARA and would have been missed had MIMS not been employed. Notably, 36% of the samples yielded significant rates of N2-fixation via ARA and also net N2-production via MIMS. In order for analysis via MIMS to show significant increases in concentrations of dissolved N2, the rates of N2-production must exceed the rates of N2-fixation in those samples. It is well documented that members of the cyanobacterial order Nostocales produce specialized cells (heterocyst) that can fix nitrogen, converting inert gaseous N2 into ammonium ion (NH4+). Thus, detecting N2-fixation in Sandusky Bay is not atypical. However, N2-production is known to occur via two pathways within the microbial nitrogen cycle (i.e., anaerobic ammonium oxidation and denitrification). Currently, neither of these N2-production pathways are known to be associated with oxygenated surface waters, but rather anoxic lake sediments (denitrification) or groundwater (anaerobic ammonium oxidation). Additional research is needed to resolve the net N2-production observed by the methods employed in this study.</p
Autochthonous versus allochthonous carbon sources of bacteria: results from whole-lake Carbon-13 addition experiments
Sources and fates of dissolved organic carbon in lakes as determined by whole-lake carbon isotope additions
Four whole- lake inorganic C-13 addition experiments were conducted in lakes of differing trophic status. Inorganic C-13 addition enriched algal carbon in C-13 and changed the delta C-13- DOC by + 1.5 parts per thousand to + 9.5 parts per thousand, depending on the specific lake. This change in delta C-13- DOC represented a significant input of algal DOC that was not completely consumed by bacteria. We modeled the dynamics in delta C-13- DOC to estimate the fluxes of algal and terrestrial carbon to and from the DOC pool, and determine the composition of the standing stock. Two experiments in lightly stained, oligotrophic lakes indicated that algal production was the source of about 20% of the DOC pool. In the following year, the experiment was repeated in one of these lakes under conditions of nutrient enrichment, and in a third, more humic lake. Algal contributions to the DOC pool were 40% in the nutrient enriched lake and 5% in the more humic lake. Spectroscopic and elemental analyses corroborated the presence of increased algal DOC in the nutrient enriched lake. Natural abundance measurements of the delta C-13 of DOC in 32 lakes also revealed the dual contributions of both terrestrial and algal carbon to DOC. From these results, we suggest an approach for inferring the contribution of algal and terrestrial DOC using easily measurable parameters