Reverse Zoonosis of Pandemic A(H1N1)pdm09 Influenza Viruses at the Swine/Human Interface

The 2009 pandemic influenza A(H1N1)pdm09 virus emerged from the swine population. Despite frequent zoonotic events, swine influenza viruses had not become established in humans previously and little is known about host-barriers which prevent swine influenza viruses from efficiently infecting humans. Thus, the emergence of the H1N1pdm09 viruses in humans and the subsequent reverse zoonoses back to swine offered an extremely valuable opportunity to expand current knowledge. We used our active swine farm surveillance platform in combination with viruses from the USDA surveillance program to look for evidence of interspecies transmission of H1N1pdm09 viruses in the US. We found phylogenetic evidence for multiple human to swine transmission events, all of which were transient suggesting that the human adapted viruses of swine origin had lost some fitness for swine. Based on our phylogenetic analysis we selected representative H1N1pdm09 viruses from the tips of swine and human sub-lineages for further study. Intriguingly, we found that after being re-introduced into the swine population, the human H1N1pdm09 viruses rapidly lost replicative fitness in human cells. Together these data provide support for a model where transmission of viruses from human to swine leads to rapid adaptation for the swine host which comes at the expense of optimal fitness for human

Can recidivism be predicted among rapists and pedophiles during their first year of parole in the state of California?

A multi-regression analysis was performed utilizing the variables, age, ethnicity, criminal background, and facets of treatment, but differentiated between the rapist and pedophile, examining the variance of recidivism

Economic Impacts of CRP on Communities

The Conservation Reserve Program (CRP) established under the Food and Security Act of 1985 had these objectives: 1) Reducing supplies of surplus agricultural commodities. 2) Providing farmer income support. 3) Preventing threats to environmental quality

Supply chain analysis of Gabilan Manufacturing Inc.

MBA Professional ReportThe purpose of this MBA Project was to investigate and provide alternative supply chain management strategies to assist Gabilan Manufacturing Inc. in reducing supply chain costs. This project was conducted with the sponsorship and assistance of Gabilan Manufacturing Inc. There were two primary goals of this project. The first was to identify and document the impact of forecasting errors in an environment where customer forecasts are available to the vendor. The second was to investigate the costs associated with relocating cutting operations as well as the procurement impact of a new cutting machine. Both of these goals relate directly to the overall effort to reduce supply chain costs without a loss of service level to Gabilan's customer.http://archive.org/details/supplychainnalys109459821Lieutenant Commander, United States NavyLieutenant, United States NavyApproved for public release; distribution is unlimited

1993 Iowa Agricultural Disaster Preliminary Estimates

On July 27, 1993 a multidisciplinary committee was formed at Iowa State University to provide preliminary estimates for the economic effects of the 1993 floods on Iowa\u27s agricultural enterprises. This report summarizes the committee\u27s initial estimates of the losses by broad economic category; detailed income information was not available at the time the report was written. The report does not provide estimates of lost income due to agribusiness work stoppages nor direct losses of livestock, buildings, fences, and machinery

Life Stage, Gender and Movement of Blue Crabs (Callinectis sapidus) in Lake Mattamuskeet and Connecting Canals

In their ranges on east and south coasts of the Americas as well as their established invasions in the Adriatic and Baltic, blue crabs, Callinectis sapidus, inhabit estuaries, sounds and coastal oceans and are commercially and ecologically important. How crabs move in response to physical variables is important to management. We monitored life stages at canal control structures, assessed gender ratios with recreational crabbing, learned from crabbers, and studied movements of tagged crabs in a canal connecting Lake Mattamuskeet to the Pamlico sound.  Juveniles enter the lake  through two of 4 canals connecting to the sounds.  Females migrate out through one canal. The lake standing population is about 70% male.  Movements of 240 crabs in August 2012 and 102 crabs in October 2014 were quantified using RFID tags with co-located meteorological and oceanographic devices. Non-spawning females and males are nomadic.  Crabs released in the canal move in response to changes in water depth and go with the flow, toward the Pamlico Sound (summer 76% and fall 78%). What crabbers describe as a fall migration  appears to be concentration of crabs in warmer deeper canals and then southern movement with flow generated by strong north winds. To be effective, management strategies like migratory corridors require understanding of crab movements

Late-Winter Abundance and Substrate Associations of Benthos in Pool 13, Upper Mississippi River

In Pool 13 on the Upper Mississippi River substantial differences were observed in macroinvertebrate abundance and substrate composition among six habitat types: tailwater, main channel, main channel border, side channel, slough, and lake. Densities were highest in slough, main channel border, and lake habitats, but the number of taxa was greatest in tailwater and main channel habitats. The abundance of 38 of the 42 identified taxa was significantly correlated with either the percentage of at least one particle size fraction or the percentage of organic matter in the substrate. Variation in substrate composition among the six habitat types appeared to influence macroinvertebrate abundance

Pandemic (H1N1) 2009 in Captive Cheetah

We describe virus isolation, full genome sequence analysis, and clinical pathology in ferrets experimentally inoculated with pandemic (H1N1) 2009 virus recovered from a clinically ill captive cheetah that had minimal human contact. Evidence of reverse zoonotic transmission by fomites underscores the substantial animal and human health implications of this virus

Profiling allele-specific gene expression in brains from individuals with autism spectrum disorder reveals preferential minor allele usage.

One fundamental but understudied mechanism of gene regulation in disease is allele-specific expression (ASE), the preferential expression of one allele. We leveraged RNA-sequencing data from human brain to assess ASE in autism spectrum disorder (ASD). When ASE is observed in ASD, the allele with lower population frequency (minor allele) is preferentially more highly expressed than the major allele, opposite to the canonical pattern. Importantly, genes showing ASE in ASD are enriched in those downregulated in ASD postmortem brains and in genes harboring de novo mutations in ASD. Two regions, 14q32 and 15q11, containing all known orphan C/D box small nucleolar RNAs (snoRNAs), are particularly enriched in shifts to higher minor allele expression. We demonstrate that this allele shifting enhances snoRNA-targeted splicing changes in ASD-related target genes in idiopathic ASD and 15q11-q13 duplication syndrome. Together, these results implicate allelic imbalance and dysregulation of orphan C/D box snoRNAs in ASD pathogenesis