Range Expansion by Moose into Coastal Temperate Rainforests of British Columbia, Canada

Ranges of species are dynamic and respond to long-term climate change and contemporary effects such as habitat modification. We report here that moose (Alces alces) have recently colonized coastal temperate rainforests of British Columbia, Canada. Contrary to recent publications, field observations of moose and their sign, combined with their occurrence in wolf (Canis lupus) faeces, suggest that moose are now widespread on the coastal mainland and occur on least three islands. Traditional ecological knowledge (information accumulated by aboriginal peoples about their environment) suggests that colonization occurred during the mid 1900s, concomitant with logging of major watersheds that bisect the Coast Mountain Range. Range expansion by moose may have ecological consequences such as alteration of predator– prey dynamics and transmission of disease to native deer (Odocoileus hemionus)

Faecal-Centric Approaches to Wildlife Ecology and Conservation; Methods, Data and Ethics

Abundant and commonly encountered in the field, wildlife faeces have long attracted scientists. Recent advances in molecular techniques, however, especially when coupled with creative study designs, can now yield a great variety of high quality data. Herein, we review the opportunities and challenges of faecal-centric approaches to address ecological and conservation questions using wolves of coastal British Columbia, Canada, as a case system. We begin by discussing methodological considerations, which should have broad applicability to any wildlife study system. We then summarize the extensive and unique variety of data that has emerged from our ‘facts from faeces’ approach with wolves, which has ranged from descriptive autecology to process-oriented hypothesis-testing to applied conservation management. We conclude by contrasting this non-invasive approach with radio-collaring in an ethics framework. We contend that when the two methods are equally efficacious in answering required research questions, scatology become the only ethical option, a perspective increasingly codified as policy governing research activities

A Critical Assessment of Protection for Key Wildlife and Salmon Habitats under the Proposed British Columbia Central Coast Land and Resource Management Plan

The Central Coast Land and Resource Management Plan (CCLRMP) table recently declared a consensus1 on proposed protected areas for British Columbia’s Central Coast. This region is recognized for its globally rare and largely intact mainland and island ecosystems and land use decisions should reflect this importance. We evaluated the efficacy of this proposal using a spatial assessment of habitat. We focus on protected areas in the context of the overall CCLRMP. We examined the level of protection provided by the CCLRMP in three key coastal habitats: deer winter range, wolf reproductive habitat, and salmon reproductive and rearing habitat. Assessment of deer winter range was limited to Heiltsuk Territory, which comprises a large proportion of the CCLRMP region

The genetic legacy of extirpation and re-colonization in Vancouver Island wolves

Hybridization between wild and domestic species is of conservation concern because it can result in the loss of adaptations and/or disappearance of a distinct taxon. Wolves from Vancouver Island, British Columbia (Canada), have been subject to several eradication campaigns during the twentieth century and were considered virtually extirpated between 1950 and 1970. In this study, we use control region mitochondrial DNA sequences and 13 autosomal microsatellite loci to characterize Vancouver Island wolves as well as dogs from British Columbia. We observe a turnover in the haplotypes of wolves sampled before and after the 1950-1970 period, when there was no permanent wolf population on the island, supporting the probable local extinction of wolves on Vancouver Island during this time, followed by re-colonization of the island by wolves from mainland British Columbia. In addition, we report the presence of a domestic dog mtDNA haplotype in three individuals eliminated in 1986 that were morphologically identified as wolves. Here we show that Vancouver Island wolves were also identified as wolves based on autosomal microsatellite data. We attribute the hybridization event to the episodically small size of this population during the re-colonization event. Our results demonstrate that at least one female hybrid offspring, resulting from a cross of a male wolf and a female dog or a female hybrid pet with dog mtDNA, successfully introgressed into the wolf population. No dog mtDNA has been previously reported in a population of wild wolves. Genetic data show that Vancouver Island wolves are distinct from dogs and thus should be recognized as a population of wild wolves. We suggest that the introgression took place due to the Allee effect, specifically a lack of mates when population size was low. Our findings exemplify how small populations are at risk of hybridization. © 2009 Springer Science+Business Media B.V.Peer Reviewe

Early Ontogenetic Diet in Gray Wolves, Canis lupus, of Coastal British Columbia

Within populations, different age classes often consume dissimilar resources, and provisioning of juveniles by adults is one mechanism by which this can occur. Although the diet of Gray Wolves (Canis lupus) has been studied extensively, the diet of pups is largely unknown. We examined faeces deposited by altricial pups and adult providers during the first two months following birth at two den sites over two years on the central coast of British Columbia, Canada. Pups and adult wolves consumed similar species, and Black-tailed Deer (Odocoileus hemionus) constituted most of the diet for both age groups. Pup and adult diet, however, diverged. Specifically, adult deer occurred significantly less frequently in the diet of pups than in the diet of adult wolves, which suggests that adults selectively provisioned pups. We speculate that this may relate to adaptive strategies of adult wolves to provide their offspring with food of optimal nutritional value or reduced parasitic burden, and/or logistic factors associated with provisioning such as prey transportability and availability

Early Ontogenetic Diet in Gray Wolves, Canis lupus, of Coastal British Columbia

Within populations, different age classes often consume dissimilar resources, and provisioning of juveniles by adults is one mechanism by which this can occur. Although the diet of Gray Wolves (Canis lupus) has been studied extensively, the diet of pups is largely unknown. We examined faeces deposited by altricial pups and adult providers during the first two months following birth at two den sites over two years on the central coast of British Columbia, Canada. Pups and adult wolves consumed similar species, and Black-tailed Deer (Odocoileus hemionus) constituted most of the diet for both age groups. Pup and adult diet, however, diverged. Specifically, adult deer occurred significantly less frequently in the diet of pups than in the diet of adult wolves, which suggests that adults selectively provisioned pups. We speculate that this may relate to adaptive strategies of adult wolves to provide their offspring with food of optimal nutritional value or reduced parasitic burden, and/or logistic factors associated with provisioning such as prey transportability and availability

Facts from Faeces: Prey Remains in Wolf, Canis lupus, Faeces Revise Occurrence Records for Mammals of British Columbia's Coastal Archipelago

Archipelagos often harbour taxa that are endemic and vulnerable to disturbance. Conservation planning and research for these areas depend fundamentally on accurate and current taxonomic inventories. Although basic ecological information is in its infancy, the temperate rainforest islands of coastal British Columbia are undergoing rapid human-caused modification, particularly logging. We report herein new mammal records for these islands as determined by prey remains in the faeces of Wolves (Canis lupus), the area’s apex mammalian terrestrial predator. Of particular interest is our detection of Marten (Martes americana) on islands previously inventoried and island occupancy by Moose (Alces alces), which have apparently migrated recently to coastal British Columbia. Remains in faeces provided valuable new species occurrence information, but more extensive and focused inventories are required to generate predictions of island occupancy by mammals based on biotic and abiotic landscape features

Maintaining Ethical Standards during Conservation Crises

Many species at risk in Canada and globally are at or approaching a crisis, especially where little or nothing consequential is being done to prevent extirpation. Such is the case of endangered boreal caribou (Rangifer tarandus caribou) in southern Alberta, Canada. Expedient but inadequate emergency ‘fixes’ have been experimentally implemented to arrest their decline and potential extirpation, but use of these measures raises important ethical problems. In their study of the effects of killing wolves (Canis lupus) on the Little Smoky woodland caribou population, Hervieux et al. (2014a) employed lethal methods that included shooting a firearm from a helicopter and the use of strychnine baits. Both of these methods raise critical questions with regard to animal welfare. When it is necessary to kill an animal, reliable humane procedures must be used to avoid pain or distress, and produce rapid loss of consciousness until death occurs. Also relevant are formal approvals by government and institutional animal ethics committees that adhere to Canadian Council on Animal Care (CCAC) guidelines. Shooting a moving animal from a helicopter is prone to error and not conducive to shots that quickly render animals insensitive to pain or produce a consistently quick kill. Strychnine does not meet the CCAC’s criteria for an acceptable killing method, and is specifically prohibited as an injectable option for euthanizing animals. Its use under uncontrolled conditions at bait sites is likely even less suitable. In addition, the risks of non-lethal and painful injuries from this poison and associated deaths to large numbers of non-target animals clearly contravene the CCAC guidelines for wildlife research. This study did not meet the CCAC’s guidelines and did not adhere to the Canadian Journal of Zoology’s requirement that all research must be approved by an institutional animal care committee. More broadly, and regardless of the failure of formal safeguards and implicit justifications offered by authors, we should be concerned when researchers impose suffering on wild animals and advocate for such programs to continue. Based on an apparent lack of compliance with CCAC’s guidelines, we believe that this controversial study should never have taken place and should not have been published by the Canadian Journal of Zoology. Experiments that involve the intentional inhumane killing of animals violate the fundamental principles of ethical science and rightfully endanger the reputation of science and scientists, as well as the journals willing to publish them. We recommend that CCAC guidelines be further developed to clearly address field methods used in wildlife studies, namely the shooting of animals from a helicopter, and the use of strychnine in baits. Also, independent audits should be conducted to investigate individual researchers and their studies, and the journals that publish this work, to ensure that CCAC guidelines are properly followed, even by researchers who collaborate well after the animal-based procedures have been carried out

Predators in Natural Fragments: Foraging Ecology of Wolves in British Columbia’s Central and North Coast Archipelago

Aim Predator–prey dynamics in fragmented areas may be influenced by spatial features of the landscape. Although little is known about these processes, an increasingly fragmented planet underscores the urgency to predict its consequences. Accordingly, our aim was to examine foraging behaviour of an apex mammalian predator, the wolf (Canis lupus), in an archipelago environment. Location Mainland and adjacent archipelago of British Columbia, Canada; a largely pristine and naturally fragmented landscape with islands of variable size and isolation. Methods We sampled 30 mainland watersheds and 29 islands for wolf faeces in summers 2000 and 2001 and identified prey remains. We examined broad geographical patterns and detailed biogeographical variables (area and isolation metrics) as they relate to prey consumed. For island data, we used Akaike Information Criteria to guide generalized linear regression model selection to predict probability of black-tailed deer (main prey; Odocoileus hemionus) in faeces. Results Black-tailed deer was the most common item in occurrence per faeces (63%) and occurrence per item (53%) indices, representing about 63% of mammalian biomass. Wolves consumed more deer on islands near the mainland (65% occurrence per item) than on the mainland (39%) and outer islands (45%), where other ungulates (mainland only) and small mammals replaced deer. On islands, the probability of detecting deer was influenced primarily by island distance to mainland (not by area or inter-landmass distance), suggesting limited recolonization by deer from source populations as a causal mechanism. Main conclusions Although sampling was limited in time, consistent patterns among islands suggest that population dynamics in isolated fragments are less stable and can result in depletion of prey. This may have important implications in understanding predator–prey communities in isolation, debate regarding wolf– deer systems and logging in temperate rain forests, and reserve design