Effects of high-dose atorvastatin treatment in plasma proteome after an acute coronary syndrome

Comunicaciones a congreso

Analysis of human erythrocyte membrane and cytosolic sub-proteomes by 2-DE

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Development of a protocol for a rat spinal cord proteome

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Profundizando en la estenósis aórtica valvular: análisis proteómico del plasma

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Identificacion of differentially expressed proteins in acute coronary syndromes

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Identification of protein expressed by aortic stenosis valves in the search for novel biomarkers

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Aproximación a la patogenia de la aterosclerosis.Análisis del secretoma de arterias humanas sanas y con distintos grados de afección

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Inside human aortic stenosis: a proteomic analysis of plasma

This work was supported by grants from the Instituto de Salud Carlos III (FIS PI070537, CP09/00229), Fondo de Investigación Sanitaria de Castilla la Mancha (FISCAM, PI2008/28 and PI2008/08) and Redes Temáticas de Investigación Cooperativa (FONDOS FEDER, RD06/0014/1015), CNIC.Valvular aortic stenosis (AS) produces a slowly progressive obstruction in left ventricular outflow track. For this reason, aortic valve replacement is warranted when the valvular stenosis is hemodinamically significant, becoming the most common worldwide cause of aortic valve surgery. Recent epidemiologic studies have revealed an association between degenerative AS and cardiovascular risk factors for atherosclerosis, althought reducing the exposure to such factors and statin therapies both fail to delay or reverse the pathology. Hence, a deeper understanding of the pathophysiology of this disease is required to identify appropriate preventive measures. A proteomic analysis of plasma will permit to know and identify the changes in protein expression induced by AS in this tissue. Using two-dimensional difference gel electrophoresis (2D-DIGE) followed by mass spectrometry (MS), we compared the crude (not pre-fractioned) and pre-fractioned plasma from AS patients and control subjects. We sought to identify plasma proteins whose expression is modified in AS. In addition we investigated if crude plasma presented some alterations in the more abundant proteins since to date, has never been studied before. We also further investigated the link between this disease and atherosclerosis with a view to identifying new potential markers and therapeutic targets.Depto. de Genética, Fisiología y MicrobiologíaFac. de Ciencias BiológicasTRUEpu