Evaluation of rK39 rapid diagnostic tests for canine visceral leishmaniasis : longitudinal study and meta-analysis

Canine visceral leishmaniasis is a vector-borne disease caused by the intracellular parasite Leishmania infantum. It is an important veterinary disease, and dogs are also the main animal reservoir for human infection. The disease is widespread in the Mediterranean area, and parts of Asia and South and Central America, and is potentially fatal in both dogs and humans unless treated. Diagnosis of canine infections requires serological or molecular tests. Detection of infection in dogs is important prior to treatment, and in epidemiological studies and control programmes, and a sensitive and specific rapid diagnostic test would be very useful. Rapid diagnostic tests (RDTs) have been developed, but their diagnostic performance has been reported to be variable. We evaluated the sensitivity of a RDT based on serological detection of the rK39 antigen in a cohort of naturally infected Brazilian dogs. The sensitivity of the test to detect infection was relatively low, but increased with time since infection and the severity of infection. We then carried out a meta-analysis of published studies of rK39 RDTs, evaluating the sensitivity to detect disease and infection. The results suggest that rK39 RDTs may be useful in a veterinary clinical setting, but the sensitivity to detect infection is too low for operational control programmes

Parallaxes of southern extremely cool objects. I. : Targets, proper motions, and first results

‘In these times, during the rise in the popularity of institutional repositories, the Society does not forbid authors from depositing their work in such repositories. However, the AAS regards the deposit of scholarly work in such repositories to be a decision of the individual scholar, as long as the individual's actions respect the diligence of the journals and their reviewers.’ Original article can be found at: http://iopscience.iop.org/ Copyright American Astronomical SocietyWe present results from the PARallaxes of Southern Extremely Cool objects ( PARSEC) program, an observational program begun in 2007 April to determine parallaxes for 122 L and 28 T southern hemisphere dwarfs using the Wide Field Imager on the ESO 2.2 m telescope. The results presented here include parallaxes of 10 targets from observations over 18 months and a first version proper motion catalog. The proper motions were obtained by combining PARSEC observations astrometrically reduced with respect to the Second US Naval Observatory CCD Astrograph Catalog, and the Two Micron All Sky Survey Point Source Catalog. The resulting median proper motion precision is 5 mas yr(-1) for 195,700 sources. The 140 0.3 deg(2) fields sample the southern hemisphere in an unbiased fashion with the exception of the galactic plane due to the small number of targets in that region. The proper motion distributions are shown to be statistically well behaved. External comparisons are also fully consistent. We will continue to update this catalog until the end of the program, and we plan to improve it including also observations from the GSC2.3 database. We present preliminary parallaxes with a 4.2 mas median precision for 10 brown dwarfs, two of which are within 10 pc. These increase the present number of L dwarfs by 20% with published parallaxes. Of the 10 targets, seven have been previously discussed in the literature: two were thought to be binary, but the PARSEC observations show them to be single; one has been confirmed as a binary companion and another has been found to be part of a binary system, both of which will make good benchmark systems. These results confirm that the foreseen precision of PARSEC can be achieved and that the large field of view will allow us to identify wide binary systems. Observations for the PARSEC program will end in early 2011 providing three to four years of coverage for all targets. The main expected outputs are: more than a 100% increase in the number of L dwarfs with parallaxes, increment in! the num ber of objects per spectral subclass up to L9-in conjunction with published results-to at least 10, and to put sensible limits on the general binary fraction of brown dwarfs. We aim to contribute significantly to the understanding of the faint end of the H-R diagram and of the L/T transition region.Peer reviewe

A Very Low-Complexity 0.3-4.4 GHz 0.004 mm2 All-Digital Ultra-Wide-Band Pulsed Transmitter for Energy Detection Receivers

This paper presents a very low-complexity all-digital IR-UWB transmitter that can generate pulses in the band 0-5 GHz, requiring a silicon area lower than a PAD for signal I/O. The trans- mitter, suited to non-standardized low data rate applications, is prototyped in a 130 nm RFCMOS technology and includes analog control signals for frequency and bandwidth tuning. Center fre- quency is linearly selected with voltage supply, 0.5 V for the range 0-960 MHz and 1.1 V supply for the higher 3.1-5 GHz range. The architecture is based on the same delay cell for both baseband and radio frequency signal generation and pulses fractional bandwidth remains constant when voltage supply and control voltages scale. At 420 MHz center frequency, the transmitter achieves 7 pJ/pulse, and for 4 GHz center frequency pulses, it achieves 32 pJ/pulse ac- tive energy consumption. The OOK/S-OOK transmitter occupies an area of 0.004 mm . For ASK modulation, the system includes a separate on-chip capacitor bank connected to the output of the transmitter for an overall size of 0.024mm2. For pulse rates below 100 kpps, the generated pulses meet the FCC indoor mask with an off-chip DC block capacitor. The paper also presents over-the-air measurements using a planar monopole antenna operating in the 1.5-3.7 GHz frequency rang

Perfluoroalkyl substances and thyroid stimulating hormone levels in a highly exposed population in the Veneto Region

Background: Per- and poly-fluoroalkyl substances (PFAS) are persistent and widespread environmental pollutants. People living in Veneto Region (Italy) have been exposed from the late 1970s to 2013 to elevated concentrations of PFAS through drinking water. The effect of PFAS on thyroid function is still controversial and studies focusing on thyroid stimulating hormone (TSH) have shown inconsistent results. The aim of this study was to evaluate the association between serum PFAS and TSH levels and its dose-response relationship in a large population of highly exposed individuals. Methods: A cross-sectional study was conducted on 21,424 individuals aged 14–39 living in the contaminated area. In the main analysis, participants with prevalent thyroid disease and pregnant women were excluded. Serum levels of perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), perfluorohexanesulfonic acid (PFHxS) and perfluorononanoic acid (PFNA) were measured. Generalized Additive Models were used to evaluate the association between TSH levels and serum PFAS, using thin plate spline smooth terms to model the potential non-linear relationship. Models were stratified by sex and age group and adjusted for potential confounders. A secondary analysis was conducted to evaluate the association between PFAS with prevalent self-reported thyroid disorders. Results: We found no association between TSH and any type of PFAS among adolescents or women. A decrease in TSH concentration was observed in association with an IQR increase in PFHxS and a mild decrease in TSH at low levels of PFOA, PFOS and PFHxS among male adults. Self-reported thyroid disease was more common among women with higher levels of PFNA concentrations, whereas all other PFAS were not associated with thyroid diseases regardless of sex or age. Conclusions: Overall there is no evidence of an association between TSH and PFAS. However, some results are suggestive of a possible inverse association of TSH with PFOA, PFOS and PFHxS among adult males

Centro Per La Ricerca

This report has been submittedforted 17881 outside of ITC and will probably be copyrighted if accepted for publication. It has been issued as a Technical Reportfort 17 dissemination of its contents. In view of the transfert of copy right tot outside publisher, its distribution outside of ITCprior7 publication should be limited to peer communications andspecific1084 4 After outside publication, material will be available onlyin 1 form authorized by the copyright owne