Develop a 3D Neurological Disease Model of Human Cortical Glutamatergic Neurons Using Micropillar-Based Scaffolds

Establishing an effective three-dimensional (3D) in vitro culture system to better model human neurological diseases is desirable, since the human brain is a 3D structure. Here, we demonstrated the development of a polydimethylsiloxane (PDMS) pillar-based 3D scaffold that mimicked the 3D microenvironment of the brain. We utilized this scaffold for the growth of human cortical glutamatergic neurons that were differentiated from human pluripotent stem cells. In comparison with the 2D culture, we demonstrated that the developed 3D culture promoted the maturation of human cortical glutamatergic neurons by showing significantly more MAP2 and less Ki67 expression. Based on this 3D culture system, we further developed an in vitro disease-like model of traumatic brain injury (TBI), which showed a robust increase of glutamate-release from the neurons, in response to mechanical impacts, recapitulating the critical pathology of TBI. The increased glutamate-release from our 3D culture model was attenuated by the treatment of neural protective drugs, memantine or nimodipine. The established 3D in vitro human neural culture system and TBI-like model may be used to facilitate mechanistic studies and drug screening for neurotrauma or other neurological diseases

The Green Manure (<i>Astragalus sinicus</i> L.) Improved Rice Yield and Quality and Changed Soil Microbial Communities of Rice in the Karst Mountains Area

The use of green manure plants for soil restoration is a viable agricultural practice that can mitigate soil degradation and biodiversity loss caused by the long-term application of inorganic fertilizers. However, the effects of green manure on soil microbial communities, rice yield, and quality in the karst mountains are largely unknown. The effects of no chemical fertilizer, chemical fertilizer, chemical fertilizer + different Astragalus sinicus L. (Chinese milk vetch, CMV) treatments on the microbial community, soil enzyme activities, soil nutrient content, and crop yield were investigated through field experiments. A moderate application of chemical fertilizer with green manure can increase chlorophyll content, increase effective rice spikes, positive impact on rice yield, and increase crude protein, etc. Additional application of the moderate amount of CMV can increase alkali-hydrolyzable nitrogen and available phosphorus (a significant increase of 54.87–72.65%), improve microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) (CFMV2 significantly increased by 22.16%), improve soil urease and phosphatase activities, and the urease activity increased by 43.43–69.24% with CMV application compared to CK. Moreover, all bacterial communities were dominated by three major phyla (Proteobacteria, Chloroflexi, and Acidobacteria), where the application of chemical fertilizer with CMV increased the ratio of abundance of Proteobacteria and Acidobacteria in rice soils, and the effect of chemical fertilizer application on the dominant bacteria was regulated to some extent by additional green manure application, which may have a beneficial effect on rice yield. Therefore, we conclude that the rational use of chemical fertilizers with CMV (22,500 kg ha−1) in karst landscapes is one of the effective measures to achieve efficient and sustainable use of rice fields

Improving Ecological Functions and Ornamental Values of Traditional Pear Orchard by Co-Planting of Green Manures of <i>Astragalus sinicus</i> L. and <i>Lathyrus cicera</i> L.

Traditional orchards received little attention in ecology. In order to enhance the ecological function of traditional pear orchard, it is an effective strategy to co-plant the ornamental green manure (GM) under the pear forest. In this study, two kinds of GM, i.e., Astragalus sinicus L. (AS) and Lathyrus cicera L. (LC), were co-planted in pear tree orchard to elevate its landscape benefits of spatiotemporal distribution of flowers, the nutrient benefits and oxygen production. The results showed that the flower height of AS and LC arrange between 20~30 cm, and the flowering period covers the March. LC has a large number of flowers, a small area of single flower, and high yield of fresh grass. AS has a small number of flowers, a large area of single flower, and low yield of a single fresh grass. Among them, 35% AS + 65% LC and 50% AS + 50% LC are more suitable in achieving the well tourism value and potential good production of pear orchard. Nutrient accumulation, total carbon fixation and oxygen production, flower number of 35% AS + 65% LC are larger than other treatments, while the flower period of 50% AS + 50% LC is longest. This study proposed a “win-win” GM planting strategy for sustainable orchard development, by enhancing ecology functions and the landscaped value of the traditional fruit orchard

Engineered Skin Substitute Regenerates the Skin with Hair Follicle Formation

Currently, engineered skin substitutes (ESS) are unable to regenerate cutaneous appendages. Recent studies have shown that skin-derived precursors (SKPs), which are extensively available, have the potential to induce hair follicle neogenesis. Here, we demonstrate that ESS consisting of culture-expanded SKPs and epidermal stem cells (Epi-SCs) reconstitute the skin with hair follicle regeneration after grafting into nude mice. SKPs seeded in a C-GAG matrix proliferated and expressed higher levels of hair induction signature genes—such as Akp2, Sox2, CD133 and Bmp6—compared to dermal fibroblasts. Moreover, when ESS prepared by seeding a mixture of culture-expanded murine SKPs and human adult Epi-SCs into a C-GAG matrix was grafted into full-thickness skin wounds in nude mice, black hairs were generated within 3 weeks. Immunofluorescence analysis showed that the SKPs were localized to the dermal papillae of the newly-formed hair follicle. Our results indicate that SKPs can serve as the hair-inductive cells in ESS to furnish it with hair genesis potentia