Histological and morphometric aspects of ridge preservation with a moldable, in situ hardening bone graft substitute

Biphasic calcium phosphates (BCP) are widely used in alveolar ridge regeneration as a porous scaffold for new bone formation. The aim of this case series was to evaluate the regenerative effect of the combination of BCP and polylactide-co-glycolide (PLGA) which can serve as a barrier membrane during bone regeneration. The study included five patients. Four months into the healing period, bone samples were collected for histological and morphometric analyses. The results of morphometric analysis showed that newly formed bone represented 32.2 ± 6.8% of the tissue, 31.9 ± 8.9% was occupied by residual graft and 35.9 ± 13.5% by soft tissue. Active osteogenesis was seen around the particles of the graft. The particles were occupied mostly by immature woven bone and connective tissue. The quality and quantity of newly formed bone, after the use of BCP/PLGA for ridge preservation, can be adequate for successful implant therapy after tooth extraction.Projekat ministarstva br. OI 175021 i br. OI 17300

Microencapsulated biofertilizer formulation: product development and effect on growth of green pepper seedlings

Aim of the study: This study aimed to formulate a novel, commercially applicable biofertilizer, to optimize the microencapsulation procedure of Bacillus subtilis NCIM 2063 and examine the stability and phytostimulatory effects of obtained formulation. Area of the study: Southestern Serbia. Material and methods: Microbial powder formulations were prepared using spray drying with maltodextrin as a carrier. The spray drying conditions were set according to Box-Benkhen experimental desing. The effect of the formulation was tested on green pepper (Capsicum annuum) seeds in controled conditions. Main results: Response surface models were developed. All of the models were statistically significant, adequately fitted and reproducible. The maximum achieved values of viability and yield in a formulation were 1.99·109 CFU/g and 96.8%, respectively, whilst the driest formulation had 1.44% moisture. The following optimum conditions were proposed for the spray drying procedure: an inlet air temperature of 133 °C, maltodextrin concentration of 50 g/L and a feed flow rate of 6.5 mL/min. The obtained microbial formulation had a high survival rate after being stored at room temperature over a 1--year period. Its application on green pepper seeds had beneficial effect on plant height, leaf dry weight and chlorophyll content of the seedlings. Research highlights: B. subtilis was successfully microencapsulated on maltodextrin as a carrier. Interaction effects between the process variables were fully explained and statistically significant models were developed. In addition to biocontrol properties formulation had a phytostimulatory effect, excellent stability and satisfactory physical properties. © 2022 CSIC

Histological and morphometric aspects of ridge preservation with a moldable, in situ hardening bone graft substitute

Biphasic calcium phosphates (BCP) are widely used in alveolar ridge regeneration as a porous scaffold for new bone formation. The aim of this case series was to evaluate the regenerative effect of the combination of BCP and polylactide-co-glycolide (PLGA) which can serve as a barrier membrane during bone regeneration. The study included five patients. Four months into the healing period, bone samples were collected for histological and morphometric analyses. The results of morphometric analysis showed that newly formed bone represented 32.2 ± 6.8% of the tissue, 31.9 ± 8.9% was occupied by residual graft and 35.9 ± 13.5% by soft tissue. Active osteogenesis was seen around the particles of the graft. The particles were occupied mostly by immature woven bone and connective tissue. The quality and quantity of newly formed bone, after the use of BCP/PLGA for ridge preservation, can be adequate for successful implant therapy after tooth extraction.Projekat ministarstva br. OI 175021 i br. OI 17300

Fabrication of silver-decorated zinc oxide microrods by hydrothermal method

We report on hydrothermal synthesis of zinc oxide (ZnO) hexagonal microrods and microtubes. Zinc oxide seeds were deposited onto glass slides by dip coating and used as templates for hydrothermal growth of ZnO microcrystals. The process was carried out in an alkaline reaction medium with acetate and nitrate zinc precursors, using hexamethylene tetramine (HMT) as surfactant. The obtained ZnO microrods were successfully decorated with silver particles via dipping ZnO-containing glass slides in silver nitrate solution and subsequent UV irradiation (5 min). Zinc oxide and Ag/ZnO microparticles were characterized by SEM, EDS and XRD. The presented results are part of the larger concept of developing of anisotropic Ag/ZnO microrods for conversion of chemical to mechanical energy.X Serbian Ceramic Society Conference - Advanced Ceramics and Application : new frontiers in multifunctional material science and processing : program and the book of abstracts; September 26-27, 2022; Belgrad

Non-exponential tunneling ionization probability distribution as a function of different laser beam profiles

In this paper, we discussed the probability distribution of exponential and non-exponential tunneling ionization of atoms, taking into account that the tunneling is not instantaneous, but requires a very short time interval. We also investigated how different laser beam profiles affected the probability distribution. These physical situations were analyzed for the valence electron of a potassium atom exposed to a strong laser field in a wide range of intensities (1012 ¡ 1015 W/cm2). We used the ADK theory formalism to compute probability distributions. The results demonstrate that the probability distribution in the non-exponential mode has a significantly lower value than in the exponential mode, calculated under the same conditions. We showed that various laser beam profiles on these probability distributions produce different tunneling time intervals

Modulation of Aroma and Sensory Properties of Prokupac Wines by a Bacillus-based Preparation Applied to Grapes Prior to Harvest

Modern viticulture requires the replacement of hazardous agrochemicals with eco-friendly, bio-based products such as microbial preparations that  enhance grape and wine quality while protecting the grapevine from pest and disease attacks. This study investigated the effects of a commercially available Bacillusbased preparation on the volatile and sensory properties of wines made from Vitis vinifera, cv. Prokupac grapes. Three different concentrations of preparation based on Bacillus subtilis Ch-13 were applied to grapevines two weeks prior to harvest. The total soluble solids in the grapes was affected by the application of B. subtilis Ch-1 and the alcohol content of the wine made from these grapes was greater. Wines madefrom the B. subtilis Ch-13-treated grapes showed an average increase in total phenolic compounds of about 27%, compared to the wine made from the untreated control grapes. The colour intensity of wines from thetreated grapes, independently of the concentration, was higher by more than 30% than for the wine from the control grape sample. The B. subtilis Ch-13 treatment also affected the content of 3-methyl-1-butanol,ethyl decanoate and ethyl octanoate in the wine, at about 35%, 40% and 20%, respectively. The latter compounds are responsible for floral and fruity aromas. Generally, wines made from the treated grapes showed similar sensorial characteristics but scored better overall than the control. Principal component analysis showed a clear differentiation between wine made from the control and that from the B. subtilis Ch-13-treated grapes. The results suggest that the application of B. subtilis Ch-13 to grapevines two weeks prior to harvest has a positive effect on wine quality

Centrifugal separation of liquid carbon dioxide from natural gas

Natural gas is becoming more and more a commodity in the global energy consumption. New technologies like the conversion from gas to liquid, contribute to this. But more than 16 % of the currently known global gas reserves cannot be produced due to severe CO2 and/or H2S contamination: (CO2 > 10% and H2S> 5%). The traditional technology of amine treatment is not able to economically remove these contaminants. The objective of this article is to investigate the possibilities of centrifugal separation to resolve the problem. After analyzing the existing situation, in the centrifugal separation of natural gas, some innovations in separators design and theory are suggested. The aim of the presented theoretical considerations is that the complex theory of separation to adapt to the needs of engineers engaged on the design, development and operation of these devices. [Projekat Ministarstva nauke Republike Srbije, br. 33001

Histological evaluation of direct pulp capping with novel nanostructural materials based on active silicate cements and Biodentine® on pulp tissue

The aim of this study was to examine the effect of Biodentine® and two new nanostructured materials based on active silicate cements on exposed tooth pulp of Vietnamese pigs. The study comprised 40 teeth in two Vietnamese pigs (24 months old). After class V cavity preparation, the pulp on each tooth was exposed using a small round bur. The following materials were applied on pulp exposures: Biodentine® (10 teeth), ALBO MPCA-I (10 teeth), and ALBO MPCA-II (10 teeth). In the control group, exposed pulp was covered with ProRoot MTA® (10 teeth). After the observation period of 28 days, the animals were sacrificed and the teeth prepared for histological analysis. Light microscope was used for the analysis of dentin bridge formation, tissue reorganization and inflammation, and the presence of bacteria in the pulp. In the group of Biodentine®, a complete dentin bridge was noted in 3 cases, while incomplete dentin bridge in the form of dental islets was detected in 4 cases. Nanostructured material ALBO-MPCA I provided complete dentin bridge formation in 5 teeth, in 3 teeth the formed dentin bridge was incomplete. ALBO MPCA-II showed complete closure of the pulp opening by dentin bridge in 4 samples, while in the same number of teeth it was incomplete. In the control group, 4 teeth showed a complete dentin bridge, whereas in 6 teeth it was incomplete. Histological analysis indicated favourable therapeutic effects of Biodentine® and the two materials ALBO-MPCA I and ALBO-MPCA II after teeth pulp capping in Vietnamese pigs. Pulp reaction was similar to that caused by ProRoot MTA®. [Projekat Ministarstva nauke Republike Srbije, br. ON172026

Histološka procena stanja zubne pulpe nakon direktnog prekrivanja novim nanostrukturnim materijalima na bazi aktivnih silikatnih cemenata i biodentina

The aim of this study was to examine the effect of Biodentine® and two new nanostructured materials based on active silicate cements on exposed tooth pulp of Vietnamese pigs. The study comprised 40 teeth in two Vietnamese pigs (24 months old). After class V cavity preparation, the pulp on each tooth was exposed using a small round bur. The following materials were applied on pulp exposures: Biodentine® (10 teeth), ALBO MPCA-I (10 teeth), and aLbO MPCA-II (10 teeth). In the control group, exposed pulp was covered with ProRoot MTA® (10 teeth). After the observation period of 28 days, the animals were sacrificed and the teeth prepared for histological analysis. Light microscope was used for the analysis of dentin bridge formation, tissue reorganization and inflammation, and the presence of bacteria in the pulp. In the group of Biodentine®, a complete dentin bridge was noted in 3 cases, while incomplete dentin bridge in the form of dental islets was detected in 4 cases. Nanostructured material ALBO-MPCA I provided complete dentin bridge formation in 5 teeth, in 3 teeth the formed dentin bridge was incomplete. ALBO MPCA-II showed complete closure of the pulp opening by dentin bridge in 4 samples, while in the same number of teeth it was incomplete. In the control group, 4 teeth showed a complete dentin bridge, whereas in 6 teeth it was incomplete. Histological analysis indicated favourable therapeutic effects of Biodentine® and the two materials ALBO-MPCA I and ALBO-MPCA II after teeth pulp capping in Vietnamese pigs. Pulp reaction was similar to that caused by ProRoot MTA®.Cilj ovog rada je bio dase ispita efekat Biodentina i dva nova nanostrukturna materijala na bazi aktivnih cilikatnih cemenata na eksponiranu pulpu zuba vijentamskih svinja. Istraživanje je obuhvatilo 40 zuba i dve vijetnamske svinje starosti 24 meseca. Na svima zubima je urađena preparacije kaviteta V klase i malim okruglim svrdlom eksponirana komora pulpe. Na perforaciju pulpe aplikovani su materijali: Biodentin (10 zuba), ALBO-MPCA I (10 zuba), ALBO-MPCA II (10 zuba). U kontrolnoj grupi pulpa je prekrivena MTA-om (10 zuba). Nakon observacionog perioda od 28 dana, životinje su žrtvovane i pripremljene za histološku analizu. Svetlosnom mikroskopijom analizirano je formiranje dentinskog mostića, postojanje reorganizacije tkiva, prisustvo inflamacije i bakterija u pulpi. Kod Biodentina kompletan dentinski mostić je zabeležen u 3 slučaja, a inkompletan dentinski mostić u vidu dentinskih ostrvaca u 4 slučaja. Nanostrukturni materijal ALBO-MPCA I je doveo do stvaranja kompletnog dentinskog mostića kod 5 zuba, a kod 3 zuba dentinski mostić je bio inkompletan. ALBO-MPCA II je pokazao kompletno zatvaranje komore pulpe dentinskim mostićem u 4 uzorka, dok je kod 4 uzorka on bio inkompletan. Kontrolni materijal MTA je kod 4 zuba imao stvoren kompletan dentinski mosti}, a u kod 6 zuba je on bio inkompletan. Histološka analiza je ukazala na povoljne terapijske efekte i Biodentina i dva novosintetisana materijala ALBO-MPCA I i ALBO-MPCA II u direktnom prekrivanju pulpezuba vijentamskih svinja. Reakcija pulpe bilaje slična onima koje je izazvao MTA